Diagnóstico da raiva: estudo comparativo entre a efetividade dos testes de cultivo celular e inoculação viral em camundongos

• Autor(a) principal: Tamura, Haruê Carolina Freire [UNESP]
• Data de Publicação: 2017
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/156712; http://www.athena.biblioteca.unesp.br/exlibris/bd/capelo/2018-07-04/000901749.pdf
• topic: Zoonoses; Técnicas de laboratório clínico; Saúde pública; Hidrofobia; Lyssavirus

Rabies is the most frightening disease for humanity, since ancient times. It is an anthropozoonosis with a percentage of lethality of almost 100%. It is characterized by causing encephalitis in mammals - the only species susceptible to the virus. It has a wide distribution around the world, presenting itself endemic in Brazil. Farm animals are the most affected, fact that brings great economic losses to the country. Laboratory diagnosis is essential for the disease confirmation in suspect cases and for the differential diagnosis for the others encephalitis, besides the control and prevention. The gold-standard diagnosis stipulated by the World Health Organization is fluorescent antibody test and confirmatory diagnosis is done by viral isolation in mice or in cell culture. This systematic review has compared the sensitivity of isolation in cell culture to the isolation in mice in the post-mortem diagnosis of rabies of different species, in order to verify the hypothesis that cell culture is a reliable alternative to replace the use of animals. Therefore, 12 scientific articles extracted from data sets of the PubMed, Biblioteca Virtual em Saúde (BVS), Scholar Google and Science Direct were analyzed. Inclusion criterias were: scientific articles published between the years of 2004 and 2016, English or Portuguese languages. Exclusion criterias were studies that did not contemplate methods of fluorescent antibody test, intracerebral inoculation in mice or in cell culture. Drawn conclusion that it is necessary to standardize the technique of isolation in cell culture for all species.