Endophytic fungi producing of esterases: evaluation in vitro of the enzymatic activity using pH indicator
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1517-83822013005000067 http://hdl.handle.net/11449/110040 |
Resumo: | A sensitive and efficient colorimetric method was optimized for detection of esterase enzymes produced by endophytic fungi for development of High-Throughput Screening (HTS). The fungi were isolated and obtained previously from plant species of Cerrado and Atlantic Forest located in areas of environmental preservation in the State of Sao Paulo / Brazil, as part of the project Chemical and biological prospecting endophytic fungi associated to plant species of Cerrado and Atlantic Forest. The compounds ethyl butyrate, ethyl acetate and methyl propionate were used as standards esters which were hydrolyzed by extracellular enzyme from endophytic fungi (EC. 3.1.1.1 -carboxylesterases) for production of carboxylic acids. Thus, the reduction of the pH increases the protonated indicator concentration (bromothymol blue), changing the color of the reaction medium (from blue to yellow), that can be observed and measured by spectrophotometry at 616 nm. The methodology with acid-base indicator was performed on 13 microorganisms, aiming Periconia atropurpurea asapotential source of esterase for biotransformation of short chain esters. The results also evidenced that this methodology showed to be efficient, fast, cheap, having low consumption of reagents and easy development, and can be applied to screen carboxylic-ester hydrolases in a large number of microorganisms. |
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Repositório Institucional da UNESP |
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Endophytic fungi producing of esterases: evaluation in vitro of the enzymatic activity using pH indicatoresterasepH indicatorenzyme activityendophytic fungiA sensitive and efficient colorimetric method was optimized for detection of esterase enzymes produced by endophytic fungi for development of High-Throughput Screening (HTS). The fungi were isolated and obtained previously from plant species of Cerrado and Atlantic Forest located in areas of environmental preservation in the State of Sao Paulo / Brazil, as part of the project Chemical and biological prospecting endophytic fungi associated to plant species of Cerrado and Atlantic Forest. The compounds ethyl butyrate, ethyl acetate and methyl propionate were used as standards esters which were hydrolyzed by extracellular enzyme from endophytic fungi (EC. 3.1.1.1 -carboxylesterases) for production of carboxylic acids. Thus, the reduction of the pH increases the protonated indicator concentration (bromothymol blue), changing the color of the reaction medium (from blue to yellow), that can be observed and measured by spectrophotometry at 616 nm. The methodology with acid-base indicator was performed on 13 microorganisms, aiming Periconia atropurpurea asapotential source of esterase for biotransformation of short chain esters. The results also evidenced that this methodology showed to be efficient, fast, cheap, having low consumption of reagents and easy development, and can be applied to screen carboxylic-ester hydrolases in a large number of microorganisms.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidade Estadual Paulista Julio de Mesquita Filho Instituto de QuímicaUniversidade Federal de Mato Grosso Instituto de Ciências Exatas e NaturaisUniversidade Estadual Paulista Julio de Mesquita Filho Instituto de QuímicaSociedade Brasileira de MicrobiologiaUniversidade Estadual Paulista (Unesp)Universidade Federal de Mato Grosso Instituto de Ciências Exatas e NaturaisLisboa, Helen Cristina Fávero [UNESP]Biasetto, Carolina Rabal [UNESP]Medeiros, João Batista De [UNESP]Araújo, Angela Regina [UNESP]Silva, Dulce Helena Siqueira [UNESP]Teles, Helder LopesTrevisan, Henrique Celso [UNESP]2014-10-01T13:08:46Z2014-10-01T13:08:46Z2013-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article923-926application/pdfhttp://dx.doi.org/10.1590/S1517-83822013005000067Brazilian Journal of Microbiology. Sociedade Brasileira de Microbiologia, v. 44, n. 3, p. 923-926, 2013.1517-8382http://hdl.handle.net/11449/11004010.1590/S1517-83822013005000067S1517-83822013000300042WOS:000332062100042S1517-83822013000300042.pdf003757905408316047020049042312480000-0001-7616-96520000-0002-1516-7765SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal of Microbiology1.8100,630info:eu-repo/semantics/openAccess2024-01-01T06:22:07Zoai:repositorio.unesp.br:11449/110040Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:52:29.042946Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Endophytic fungi producing of esterases: evaluation in vitro of the enzymatic activity using pH indicator |
title |
Endophytic fungi producing of esterases: evaluation in vitro of the enzymatic activity using pH indicator |
spellingShingle |
Endophytic fungi producing of esterases: evaluation in vitro of the enzymatic activity using pH indicator Lisboa, Helen Cristina Fávero [UNESP] esterase pH indicator enzyme activity endophytic fungi |
title_short |
Endophytic fungi producing of esterases: evaluation in vitro of the enzymatic activity using pH indicator |
title_full |
Endophytic fungi producing of esterases: evaluation in vitro of the enzymatic activity using pH indicator |
title_fullStr |
Endophytic fungi producing of esterases: evaluation in vitro of the enzymatic activity using pH indicator |
title_full_unstemmed |
Endophytic fungi producing of esterases: evaluation in vitro of the enzymatic activity using pH indicator |
title_sort |
Endophytic fungi producing of esterases: evaluation in vitro of the enzymatic activity using pH indicator |
author |
Lisboa, Helen Cristina Fávero [UNESP] |
author_facet |
Lisboa, Helen Cristina Fávero [UNESP] Biasetto, Carolina Rabal [UNESP] Medeiros, João Batista De [UNESP] Araújo, Angela Regina [UNESP] Silva, Dulce Helena Siqueira [UNESP] Teles, Helder Lopes Trevisan, Henrique Celso [UNESP] |
author_role |
author |
author2 |
Biasetto, Carolina Rabal [UNESP] Medeiros, João Batista De [UNESP] Araújo, Angela Regina [UNESP] Silva, Dulce Helena Siqueira [UNESP] Teles, Helder Lopes Trevisan, Henrique Celso [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Federal de Mato Grosso Instituto de Ciências Exatas e Naturais |
dc.contributor.author.fl_str_mv |
Lisboa, Helen Cristina Fávero [UNESP] Biasetto, Carolina Rabal [UNESP] Medeiros, João Batista De [UNESP] Araújo, Angela Regina [UNESP] Silva, Dulce Helena Siqueira [UNESP] Teles, Helder Lopes Trevisan, Henrique Celso [UNESP] |
dc.subject.por.fl_str_mv |
esterase pH indicator enzyme activity endophytic fungi |
topic |
esterase pH indicator enzyme activity endophytic fungi |
description |
A sensitive and efficient colorimetric method was optimized for detection of esterase enzymes produced by endophytic fungi for development of High-Throughput Screening (HTS). The fungi were isolated and obtained previously from plant species of Cerrado and Atlantic Forest located in areas of environmental preservation in the State of Sao Paulo / Brazil, as part of the project Chemical and biological prospecting endophytic fungi associated to plant species of Cerrado and Atlantic Forest. The compounds ethyl butyrate, ethyl acetate and methyl propionate were used as standards esters which were hydrolyzed by extracellular enzyme from endophytic fungi (EC. 3.1.1.1 -carboxylesterases) for production of carboxylic acids. Thus, the reduction of the pH increases the protonated indicator concentration (bromothymol blue), changing the color of the reaction medium (from blue to yellow), that can be observed and measured by spectrophotometry at 616 nm. The methodology with acid-base indicator was performed on 13 microorganisms, aiming Periconia atropurpurea asapotential source of esterase for biotransformation of short chain esters. The results also evidenced that this methodology showed to be efficient, fast, cheap, having low consumption of reagents and easy development, and can be applied to screen carboxylic-ester hydrolases in a large number of microorganisms. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-09-01 2014-10-01T13:08:46Z 2014-10-01T13:08:46Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1517-83822013005000067 Brazilian Journal of Microbiology. Sociedade Brasileira de Microbiologia, v. 44, n. 3, p. 923-926, 2013. 1517-8382 http://hdl.handle.net/11449/110040 10.1590/S1517-83822013005000067 S1517-83822013000300042 WOS:000332062100042 S1517-83822013000300042.pdf 0037579054083160 4702004904231248 0000-0001-7616-9652 0000-0002-1516-7765 |
url |
http://dx.doi.org/10.1590/S1517-83822013005000067 http://hdl.handle.net/11449/110040 |
identifier_str_mv |
Brazilian Journal of Microbiology. Sociedade Brasileira de Microbiologia, v. 44, n. 3, p. 923-926, 2013. 1517-8382 10.1590/S1517-83822013005000067 S1517-83822013000300042 WOS:000332062100042 S1517-83822013000300042.pdf 0037579054083160 4702004904231248 0000-0001-7616-9652 0000-0002-1516-7765 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Journal of Microbiology 1.810 0,630 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
923-926 application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129368816353280 |