Metabolomic study of the chemical composition of bamboos from the genus Guadua (Poaceae) and exploration of their biological potential

Detalhes bibliográficos
Autor(a) principal: Chitiva, Luis Carlos Chitiva [UNESP]
Data de Publicação: 2024
Tipo de documento: Tese
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: https://hdl.handle.net/11449/257034
http://lattes.cnpq.br/8935150467809395
https://orcid.org/0000-0003-1422-5545
Resumo: Bamboos belonging to the Guadua genus are recognized worldwide for their various applications in the ecological, social, economic, cultural, and medicinal fields. However, to date information on its chemical composition and biological activity is still unknown. In this context, the present work aimed to study the chemical composition and biological activity of bamboos of the Guadua genus through a metabolomic approach, with four main objectives: (1) evaluate the variations in the chemical composition under the influence of an altitudinal gradient and map chemical diversity using GNPS; (2) correlate chemical composition and biological activities for the identification of bioactive compounds; (3) evaluate metabolic alterations in HCT-116 cells after exposure to G. incana extract; and (4) isolate some bioactive compounds from the active extract of G. incana, guided by the metabolomic approach. The results obtained highlight that the first objective shows a significant increase in flavonoid composition at high altitudes. A great richness in the chemical diversity of these bamboos was also demonstrated. In the second objective, the presence of 49 compounds was identified in Guadua species, including flavonoids and cinnamic acid derivatives that showed a strong correlation with anti-inflammatory and antioxidant activities. Particularly, it was found that flavone glycosides presented a positive correlation with cytotoxic potential. In the third objective, an alteration of 99 metabolites was found in HCT-116 cells when treated with the active extract of G. incana, mainly associated with glutathione metabolism. Finally, in the fourth objective, a flavone glycoside corresponding to isoschaftoside was isolated that showed potent cytotoxic activity and a mixture of three flavones derived from apigenin. In conclusion, this study contributed important and robust scientific data to the knowledge of the chemical composition and the antioxidant, anti-inflammatory and cytotoxic potential of several species of the genus Guadua that had not yet been studied.
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spelling Metabolomic study of the chemical composition of bamboos from the genus Guadua (Poaceae) and exploration of their biological potentialEstudo metabolômico da composição química de bambus do gênero Guadua (Poaceae) e exploração do seu potencial biológicoEstudio metabolómico de la composición química de bambúes del género Guadua (Poaceae) y exploración de su potencial biológicoBambooGuaduaMetabolomicsMetabolitesBioactive compoundsBambuMetabolômicaMetabólitosCompostos bioativosBamboos belonging to the Guadua genus are recognized worldwide for their various applications in the ecological, social, economic, cultural, and medicinal fields. However, to date information on its chemical composition and biological activity is still unknown. In this context, the present work aimed to study the chemical composition and biological activity of bamboos of the Guadua genus through a metabolomic approach, with four main objectives: (1) evaluate the variations in the chemical composition under the influence of an altitudinal gradient and map chemical diversity using GNPS; (2) correlate chemical composition and biological activities for the identification of bioactive compounds; (3) evaluate metabolic alterations in HCT-116 cells after exposure to G. incana extract; and (4) isolate some bioactive compounds from the active extract of G. incana, guided by the metabolomic approach. The results obtained highlight that the first objective shows a significant increase in flavonoid composition at high altitudes. A great richness in the chemical diversity of these bamboos was also demonstrated. In the second objective, the presence of 49 compounds was identified in Guadua species, including flavonoids and cinnamic acid derivatives that showed a strong correlation with anti-inflammatory and antioxidant activities. Particularly, it was found that flavone glycosides presented a positive correlation with cytotoxic potential. In the third objective, an alteration of 99 metabolites was found in HCT-116 cells when treated with the active extract of G. incana, mainly associated with glutathione metabolism. Finally, in the fourth objective, a flavone glycoside corresponding to isoschaftoside was isolated that showed potent cytotoxic activity and a mixture of three flavones derived from apigenin. In conclusion, this study contributed important and robust scientific data to the knowledge of the chemical composition and the antioxidant, anti-inflammatory and cytotoxic potential of several species of the genus Guadua that had not yet been studied.Os bambus pertencentes ao gênero Guadua são reconhecidos mundialmente por suas diversas aplicações nas áreas ecológica, social, econômica, cultural e medicinal. Entretanto, até o momento, informações sobre sua composição química e atividade biológica ainda são desconhecidas. Neste contexto o presente trabalho teve como objetivo estudar a composição química e a atividade biológica de bambus do gênero Guadua através de uma abordagem metabolômica tendo quatro objetivos principais: (1) avaliar as variações na composição química sob a influência de um gradiente altitudinal e mapear a diversidade química usando GNPS; (2) correlacionar composição química e atividades biológicas para identificação de compostos bioativos; (3) avaliar alterações metabólicas em células HCT-116 após exposição ao extrato de G. incana; e (4) isolar alguns compostos bioativos do extrato ativo de G. incana, guiado pela abordagem metabolômica. Os resultados obtidos destacam que o primeiro objetivo destaca um aumento significativo na composição de flavonóides em grandes altitudes. Também foi demonstrada uma grande riqueza na diversidade química desses bambus. No segundo objetivo, foi identificada a presença de 49 compostos nas espécies de Guadua, incluindo flavonóides e derivados do ácido cinâmico que apresentaram forte correlação com atividades antiinflamatórias e antioxidantes. Particularmente, constatou-se que as flavonas glicosiladas apresentaram correlação positiva com o potencial citotóxico. No terceiro objetivo, foi encontrada alteração de 99 metabólitos em células HCT-116 quando tratadas com o extrato ativo de G. incana, principalmente associado ao metabolismo da glutationa. Por fim, no quarto objetivo, foram isoladas uma flavona glicosilada correspondente ao isoschaftosídeo que apresentou potente atividade citotóxica e uma mistura de três flavonas derivadas da apigenina. Concluindo, este estudo contribuiu com dados científicos importantes e robustos para o conhecimento da composição química e do potencial antioxidante, antiinflamatório e citotóxico de diversas espécies do gênero Guadua que ainda não haviam sido estudadas.Los bambúes pertenecientes al género Guadua son reconocidos a nivel mundial por sus diversas aplicaciones en los ámbitos ecológico, social, económico, cultural y medicinal. Sin embargo, hasta la fecha aún se desconoce información sobre su composición química y actividad biológica. En este contexto, el presente trabajo tuvo como objetivo estudiar la composición química y actividad biológica de bambúes del género Guadua mediante un enfoque metabolómico, con cuatro objetivos principales: (1) evaluar las variaciones en la composición química bajo la influencia de un gradiente altitudinal y mapear la diversidad química mediante GNPS; (2) correlacionar la composición química y actividades biológicas para la identificación de compuestos bioactivos; (3) evaluar las alteraciones metabólicas en las células HCT-116 tras la exposición al extracto de G. incana; y (4) aislar algunos compuestos bioactivos a partir del extracto activo de G. incana, guiado por el enfoque metabolómico. Los resultados obtenidos resaltan que en el primer objetivo se destaca un aumento significativo en la composición de flavonoides a altitudes elevadas. También se demostró una gran riqueza en la diversidad química de estos bambúes. En el segundo objetivo, se identificó la presencia de 49 compuestos en las especies de Guadua, incluyendo flavonoides y derivados de ácidos cinámicos que mostraron una fuerte correlación con las actividades antiinflamatoria y antioxidante. Particularmente, se encontró que flavonas glucosiladas presentaron una correlación positiva con el potencial citotóxico. En el tercer objetivo, se encontraron una alteración de 99 metabolitos en las células HCT-116 cuando fueron tratadas con el extracto activo de G. incana, asociados principalmente con el metabolismo del glutatión. Finalmente, en el cuarto objetivo se aisló una flavona glucosilada correspondiente a isoschaftosido que mostró una potente actividad citotóxica y una mezcla de tres flavonas derivadas de apigenina. En conclusión, este estudio aportó importantes y robustos datos científicos al conocimiento de la composición química y del potencial antioxidante, antiinflamatorio y citotóxico de diversas especies del género Guadua que aún no habían sido estudiadas.OutraCNPq: 23038.000776/2017-54Universidade Estadual Paulista (Unesp)Gamboa, Ian Castro [UNESP]Costa, Geison ModestiPontificia Universidad Javeriana (Puj)Prieto-Rodríguez, Juliet AngélicaChitiva, Luis Carlos Chitiva [UNESP]2024-08-14T11:03:19Z2024-08-14T11:03:19Z2024-07-19info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdfhttps://hdl.handle.net/11449/25703433004030072P8http://lattes.cnpq.br/8935150467809395https://orcid.org/0000-0003-1422-5545enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESP2024-08-15T06:52:45Zoai:repositorio.unesp.br:11449/257034Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-15T06:52:45Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Metabolomic study of the chemical composition of bamboos from the genus Guadua (Poaceae) and exploration of their biological potential
Estudo metabolômico da composição química de bambus do gênero Guadua (Poaceae) e exploração do seu potencial biológico
Estudio metabolómico de la composición química de bambúes del género Guadua (Poaceae) y exploración de su potencial biológico
title Metabolomic study of the chemical composition of bamboos from the genus Guadua (Poaceae) and exploration of their biological potential
spellingShingle Metabolomic study of the chemical composition of bamboos from the genus Guadua (Poaceae) and exploration of their biological potential
Chitiva, Luis Carlos Chitiva [UNESP]
Bamboo
Guadua
Metabolomics
Metabolites
Bioactive compounds
Bambu
Metabolômica
Metabólitos
Compostos bioativos
title_short Metabolomic study of the chemical composition of bamboos from the genus Guadua (Poaceae) and exploration of their biological potential
title_full Metabolomic study of the chemical composition of bamboos from the genus Guadua (Poaceae) and exploration of their biological potential
title_fullStr Metabolomic study of the chemical composition of bamboos from the genus Guadua (Poaceae) and exploration of their biological potential
title_full_unstemmed Metabolomic study of the chemical composition of bamboos from the genus Guadua (Poaceae) and exploration of their biological potential
title_sort Metabolomic study of the chemical composition of bamboos from the genus Guadua (Poaceae) and exploration of their biological potential
author Chitiva, Luis Carlos Chitiva [UNESP]
author_facet Chitiva, Luis Carlos Chitiva [UNESP]
author_role author
dc.contributor.none.fl_str_mv Gamboa, Ian Castro [UNESP]
Costa, Geison Modesti
Pontificia Universidad Javeriana (Puj)
Prieto-Rodríguez, Juliet Angélica
dc.contributor.author.fl_str_mv Chitiva, Luis Carlos Chitiva [UNESP]
dc.subject.por.fl_str_mv Bamboo
Guadua
Metabolomics
Metabolites
Bioactive compounds
Bambu
Metabolômica
Metabólitos
Compostos bioativos
topic Bamboo
Guadua
Metabolomics
Metabolites
Bioactive compounds
Bambu
Metabolômica
Metabólitos
Compostos bioativos
description Bamboos belonging to the Guadua genus are recognized worldwide for their various applications in the ecological, social, economic, cultural, and medicinal fields. However, to date information on its chemical composition and biological activity is still unknown. In this context, the present work aimed to study the chemical composition and biological activity of bamboos of the Guadua genus through a metabolomic approach, with four main objectives: (1) evaluate the variations in the chemical composition under the influence of an altitudinal gradient and map chemical diversity using GNPS; (2) correlate chemical composition and biological activities for the identification of bioactive compounds; (3) evaluate metabolic alterations in HCT-116 cells after exposure to G. incana extract; and (4) isolate some bioactive compounds from the active extract of G. incana, guided by the metabolomic approach. The results obtained highlight that the first objective shows a significant increase in flavonoid composition at high altitudes. A great richness in the chemical diversity of these bamboos was also demonstrated. In the second objective, the presence of 49 compounds was identified in Guadua species, including flavonoids and cinnamic acid derivatives that showed a strong correlation with anti-inflammatory and antioxidant activities. Particularly, it was found that flavone glycosides presented a positive correlation with cytotoxic potential. In the third objective, an alteration of 99 metabolites was found in HCT-116 cells when treated with the active extract of G. incana, mainly associated with glutathione metabolism. Finally, in the fourth objective, a flavone glycoside corresponding to isoschaftoside was isolated that showed potent cytotoxic activity and a mixture of three flavones derived from apigenin. In conclusion, this study contributed important and robust scientific data to the knowledge of the chemical composition and the antioxidant, anti-inflammatory and cytotoxic potential of several species of the genus Guadua that had not yet been studied.
publishDate 2024
dc.date.none.fl_str_mv 2024-08-14T11:03:19Z
2024-08-14T11:03:19Z
2024-07-19
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/11449/257034
33004030072P8
http://lattes.cnpq.br/8935150467809395
https://orcid.org/0000-0003-1422-5545
url https://hdl.handle.net/11449/257034
http://lattes.cnpq.br/8935150467809395
https://orcid.org/0000-0003-1422-5545
identifier_str_mv 33004030072P8
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual Paulista (Unesp)
publisher.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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