Effect of complexed trace minerals on cumulus-oocyte complex recovery and in vitro embryo production in beef cattle
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1093/jas/skz005 http://hdl.handle.net/11449/187542 |
Resumo: | The objective of this experiment was to evaluate the impact of complexed trace mineral supplementation on ovum pick-up (OPU) and in vitro embryo production in lactating beef cows. Thirty days prior to fixed-time artificial insemination (FTAI; day â '30), 68 postpartum cows were stratified by BW, BCS, and parity before being randomly assigned to 10 pens of either a treatment (TRT; n = 5) or a control (CNT; n = 5) group. Each group received a weekly mineral supplement allotment of 1.16 kg × week â '1 × cow-calf pair â '1 for 14 wk. Cows assigned to the TRT group received a mineral supplement that contained amino acid complexes of zinc, copper, and manganese, as well as cobalt glucoheptonate (Availa Plus; Zinpro Corp., Eden Prairie, MN, USA), while cows assigned to the CNT group received a mineral supplement that was formulated to contain similar concentrations of these trace minerals from inorganic sources. All cows were submitted to a 7 d CO-Synch + CIDR protocol on day â '10 and bred using FTAI on day 0. Pregnancy diagnosis was performed on day 28 and nonpregnant cows were removed. All pregnant cows were subjected to ovum pick-up (OPU) on day 52 and 67 of gestation. Cumulus-oocyte complexes (COCs) were evaluated and graded prior to in vitro fertilization (IVF). Analysis of variance was conducted to determine effects of treatment on response variables, and pen was considered the experimental unit. Supplement consumption did not differ (P = 0.48) between treatments (1.16 ± 0.12 vs. 1.07 ± 0.15 kg of DM × week â '1 × cow-calf pair â '1 for TRT and CNT, respectively). Total COC recovery was greater (P = 0.03) from TRT when compared with CNT cows (22.4 ± 2.0 vs. 16.4 ± 1.4 COCs × pen â '1, respectively) and the number of COCs meeting maturation criteria was increased in TRT cows (P = 0.05) when compared with CNT cows (15.9 ± 1.6 vs. 11.8 ± 1.0 COCs × pen â '1, respectively). Production of transferable embryos tended to be greater (P = 0.06) for TRT than CNT cows (4.7 ± 0.6 vs. 2.7 ± 0.7 embryos × pen â '1, respectively). Furthermore, when expressed as a ratio, the number of recovered COCs meeting maturation criteria that were required to produce a transferable embryo tended to be lower for TRT than CNT cows (3.10 ± 0.93 vs. 7.02 ± 1.60; P = 0.06). In summary, complete replacement with complexed trace mineral improved COC recovery and in vitro embryo production when compared with inorganic forms of these trace minerals in beef cows. |
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Effect of complexed trace minerals on cumulus-oocyte complex recovery and in vitro embryo production in beef cattlebeef cattlecomplexedembryo transferin vitro fertilizationreproductiontrace mineralThe objective of this experiment was to evaluate the impact of complexed trace mineral supplementation on ovum pick-up (OPU) and in vitro embryo production in lactating beef cows. Thirty days prior to fixed-time artificial insemination (FTAI; day â '30), 68 postpartum cows were stratified by BW, BCS, and parity before being randomly assigned to 10 pens of either a treatment (TRT; n = 5) or a control (CNT; n = 5) group. Each group received a weekly mineral supplement allotment of 1.16 kg × week â '1 × cow-calf pair â '1 for 14 wk. Cows assigned to the TRT group received a mineral supplement that contained amino acid complexes of zinc, copper, and manganese, as well as cobalt glucoheptonate (Availa Plus; Zinpro Corp., Eden Prairie, MN, USA), while cows assigned to the CNT group received a mineral supplement that was formulated to contain similar concentrations of these trace minerals from inorganic sources. All cows were submitted to a 7 d CO-Synch + CIDR protocol on day â '10 and bred using FTAI on day 0. Pregnancy diagnosis was performed on day 28 and nonpregnant cows were removed. All pregnant cows were subjected to ovum pick-up (OPU) on day 52 and 67 of gestation. Cumulus-oocyte complexes (COCs) were evaluated and graded prior to in vitro fertilization (IVF). Analysis of variance was conducted to determine effects of treatment on response variables, and pen was considered the experimental unit. Supplement consumption did not differ (P = 0.48) between treatments (1.16 ± 0.12 vs. 1.07 ± 0.15 kg of DM × week â '1 × cow-calf pair â '1 for TRT and CNT, respectively). Total COC recovery was greater (P = 0.03) from TRT when compared with CNT cows (22.4 ± 2.0 vs. 16.4 ± 1.4 COCs × pen â '1, respectively) and the number of COCs meeting maturation criteria was increased in TRT cows (P = 0.05) when compared with CNT cows (15.9 ± 1.6 vs. 11.8 ± 1.0 COCs × pen â '1, respectively). Production of transferable embryos tended to be greater (P = 0.06) for TRT than CNT cows (4.7 ± 0.6 vs. 2.7 ± 0.7 embryos × pen â '1, respectively). Furthermore, when expressed as a ratio, the number of recovered COCs meeting maturation criteria that were required to produce a transferable embryo tended to be lower for TRT than CNT cows (3.10 ± 0.93 vs. 7.02 ± 1.60; P = 0.06). In summary, complete replacement with complexed trace mineral improved COC recovery and in vitro embryo production when compared with inorganic forms of these trace minerals in beef cows.Department of Animal Science University of TennesseeDepartamento de Producaõ Animal Universidade Estadual Paulista-UNESPZinpro CorporationDepartment of Animal Science Texas AandM UniversityDepartamento de Producaõ Animal Universidade Estadual Paulista-UNESPUniversity of TennesseeUniversidade Estadual Paulista (Unesp)Zinpro CorporationTexas AandM UniversityDantas, Felipe G.Reese, Sydney T.Filho, Ramiro V.O.Carvalho, Rafael S. [UNESP]Franco, G. A.Abbott, Chelsea R.Payton, Rebecca R.Edwards, J. LannettRussell, Jason R.Smith, Jason K.Pohler, Ky G.2019-10-06T15:39:34Z2019-10-06T15:39:34Z2019-04-03info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1478-1490http://dx.doi.org/10.1093/jas/skz005Journal of Animal Science, v. 97, n. 4, p. 1478-1490, 2019.1525-31630021-8812http://hdl.handle.net/11449/18754210.1093/jas/skz0052-s2.0-85064107997Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Animal Scienceinfo:eu-repo/semantics/openAccess2021-10-23T20:19:26Zoai:repositorio.unesp.br:11449/187542Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:38:59.424151Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effect of complexed trace minerals on cumulus-oocyte complex recovery and in vitro embryo production in beef cattle |
title |
Effect of complexed trace minerals on cumulus-oocyte complex recovery and in vitro embryo production in beef cattle |
spellingShingle |
Effect of complexed trace minerals on cumulus-oocyte complex recovery and in vitro embryo production in beef cattle Dantas, Felipe G. beef cattle complexed embryo transfer in vitro fertilization reproduction trace mineral |
title_short |
Effect of complexed trace minerals on cumulus-oocyte complex recovery and in vitro embryo production in beef cattle |
title_full |
Effect of complexed trace minerals on cumulus-oocyte complex recovery and in vitro embryo production in beef cattle |
title_fullStr |
Effect of complexed trace minerals on cumulus-oocyte complex recovery and in vitro embryo production in beef cattle |
title_full_unstemmed |
Effect of complexed trace minerals on cumulus-oocyte complex recovery and in vitro embryo production in beef cattle |
title_sort |
Effect of complexed trace minerals on cumulus-oocyte complex recovery and in vitro embryo production in beef cattle |
author |
Dantas, Felipe G. |
author_facet |
Dantas, Felipe G. Reese, Sydney T. Filho, Ramiro V.O. Carvalho, Rafael S. [UNESP] Franco, G. A. Abbott, Chelsea R. Payton, Rebecca R. Edwards, J. Lannett Russell, Jason R. Smith, Jason K. Pohler, Ky G. |
author_role |
author |
author2 |
Reese, Sydney T. Filho, Ramiro V.O. Carvalho, Rafael S. [UNESP] Franco, G. A. Abbott, Chelsea R. Payton, Rebecca R. Edwards, J. Lannett Russell, Jason R. Smith, Jason K. Pohler, Ky G. |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
University of Tennessee Universidade Estadual Paulista (Unesp) Zinpro Corporation Texas AandM University |
dc.contributor.author.fl_str_mv |
Dantas, Felipe G. Reese, Sydney T. Filho, Ramiro V.O. Carvalho, Rafael S. [UNESP] Franco, G. A. Abbott, Chelsea R. Payton, Rebecca R. Edwards, J. Lannett Russell, Jason R. Smith, Jason K. Pohler, Ky G. |
dc.subject.por.fl_str_mv |
beef cattle complexed embryo transfer in vitro fertilization reproduction trace mineral |
topic |
beef cattle complexed embryo transfer in vitro fertilization reproduction trace mineral |
description |
The objective of this experiment was to evaluate the impact of complexed trace mineral supplementation on ovum pick-up (OPU) and in vitro embryo production in lactating beef cows. Thirty days prior to fixed-time artificial insemination (FTAI; day â '30), 68 postpartum cows were stratified by BW, BCS, and parity before being randomly assigned to 10 pens of either a treatment (TRT; n = 5) or a control (CNT; n = 5) group. Each group received a weekly mineral supplement allotment of 1.16 kg × week â '1 × cow-calf pair â '1 for 14 wk. Cows assigned to the TRT group received a mineral supplement that contained amino acid complexes of zinc, copper, and manganese, as well as cobalt glucoheptonate (Availa Plus; Zinpro Corp., Eden Prairie, MN, USA), while cows assigned to the CNT group received a mineral supplement that was formulated to contain similar concentrations of these trace minerals from inorganic sources. All cows were submitted to a 7 d CO-Synch + CIDR protocol on day â '10 and bred using FTAI on day 0. Pregnancy diagnosis was performed on day 28 and nonpregnant cows were removed. All pregnant cows were subjected to ovum pick-up (OPU) on day 52 and 67 of gestation. Cumulus-oocyte complexes (COCs) were evaluated and graded prior to in vitro fertilization (IVF). Analysis of variance was conducted to determine effects of treatment on response variables, and pen was considered the experimental unit. Supplement consumption did not differ (P = 0.48) between treatments (1.16 ± 0.12 vs. 1.07 ± 0.15 kg of DM × week â '1 × cow-calf pair â '1 for TRT and CNT, respectively). Total COC recovery was greater (P = 0.03) from TRT when compared with CNT cows (22.4 ± 2.0 vs. 16.4 ± 1.4 COCs × pen â '1, respectively) and the number of COCs meeting maturation criteria was increased in TRT cows (P = 0.05) when compared with CNT cows (15.9 ± 1.6 vs. 11.8 ± 1.0 COCs × pen â '1, respectively). Production of transferable embryos tended to be greater (P = 0.06) for TRT than CNT cows (4.7 ± 0.6 vs. 2.7 ± 0.7 embryos × pen â '1, respectively). Furthermore, when expressed as a ratio, the number of recovered COCs meeting maturation criteria that were required to produce a transferable embryo tended to be lower for TRT than CNT cows (3.10 ± 0.93 vs. 7.02 ± 1.60; P = 0.06). In summary, complete replacement with complexed trace mineral improved COC recovery and in vitro embryo production when compared with inorganic forms of these trace minerals in beef cows. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T15:39:34Z 2019-10-06T15:39:34Z 2019-04-03 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1093/jas/skz005 Journal of Animal Science, v. 97, n. 4, p. 1478-1490, 2019. 1525-3163 0021-8812 http://hdl.handle.net/11449/187542 10.1093/jas/skz005 2-s2.0-85064107997 |
url |
http://dx.doi.org/10.1093/jas/skz005 http://hdl.handle.net/11449/187542 |
identifier_str_mv |
Journal of Animal Science, v. 97, n. 4, p. 1478-1490, 2019. 1525-3163 0021-8812 10.1093/jas/skz005 2-s2.0-85064107997 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Animal Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1478-1490 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129231838773248 |