Use of a synthetic lethal screen to identify genes related to TIF51A in Saccharomyces cerevisiae
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://www.geneticsmr.com//year2007/vol6-1/pdf/gmr0282.pdf http://hdl.handle.net/11449/69668 |
Resumo: | The putative eukaryotic translation initiation factor 5A (eIF5A) is an essential protein for cell viability and the only cellular protein known to contain the unusual amino acid residue hypusine. eIF5A has been implicated in translation initiation, cell proliferation, nucleocytoplasmic transport, mRNA decay, and actin polarization, but the precise biological function of this protein is not clear. However, eIF5A was recently shown to be directly involved with the translational machinery. A screen for synthetic lethal mutations was carried out with one of the temperature-sensitive alleles of TIF51A (tif51A-3) to identify factors that functionally interact with eIF5A and revealed the essential gene YPT1. This gene encodes a small GTPase, a member of the rab family involved with secretion, acting in the vesicular trafficking between endoplasmatic reticulum and the Golgi. Thus, the synthetic lethality between TIF51A and YPT1 may reveal the connection between translation and the polarized distribution of membrane components, suggesting that these proteins work together in the cell to guarantee proper protein synthesis and secretion necessary for correct bud formation during G1/ S transition. Future studies will investigate the functional interaction between eIF5A and Ypt1 in order to clarify this involvement of eIF5A with vesicular trafficking. ©FUNPEC-RP. |
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Use of a synthetic lethal screen to identify genes related to TIF51A in Saccharomyces cerevisiaeeIF5AGenetic interactionSynthetic lethalityVesicular traffickingYpt1guanosine triphosphataseinitiation factor 5ARab proteinsynthetic DNAallelebuddingcell proliferationgene identificationgene mutationgenetic screeninglethal genenonhumannucleocytoplasmic transportprotein secretionprotein synthesisSaccharomyces cerevisiaetranslation initiationG1 PhaseGenes, LethalMutationPeptide Initiation Factorsrab GTP-Binding ProteinsRNA-Binding ProteinsS PhaseSaccharomyces cerevisiae ProteinsTransport VesiclesEukaryotaThe putative eukaryotic translation initiation factor 5A (eIF5A) is an essential protein for cell viability and the only cellular protein known to contain the unusual amino acid residue hypusine. eIF5A has been implicated in translation initiation, cell proliferation, nucleocytoplasmic transport, mRNA decay, and actin polarization, but the precise biological function of this protein is not clear. However, eIF5A was recently shown to be directly involved with the translational machinery. A screen for synthetic lethal mutations was carried out with one of the temperature-sensitive alleles of TIF51A (tif51A-3) to identify factors that functionally interact with eIF5A and revealed the essential gene YPT1. This gene encodes a small GTPase, a member of the rab family involved with secretion, acting in the vesicular trafficking between endoplasmatic reticulum and the Golgi. Thus, the synthetic lethality between TIF51A and YPT1 may reveal the connection between translation and the polarized distribution of membrane components, suggesting that these proteins work together in the cell to guarantee proper protein synthesis and secretion necessary for correct bud formation during G1/ S transition. Future studies will investigate the functional interaction between eIF5A and Ypt1 in order to clarify this involvement of eIF5A with vesicular trafficking. ©FUNPEC-RP.Departamento de Ciências Biológicas Faculdade de Ciências Farmacêuticas UNESP, Araraquara, SPDepartamento de Ciências Biológicas Faculdade de Ciências Farmacêuticas UNESP, Araraquara, SPUniversidade Estadual Paulista (Unesp)Frigieri, M. C. [UNESP]Thompson, G. M. [UNESP]Pandolfi, J. R. [UNESP]Zanelli, Cleslei Fernando [UNESP]Valentini, Sandro Roberto [UNESP]2014-05-27T11:22:28Z2014-05-27T11:22:28Z2007-05-18info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article152-165application/pdfhttp://www.geneticsmr.com//year2007/vol6-1/pdf/gmr0282.pdfGenetics and Molecular Research, v. 6, n. 1, p. 152-165, 2007.1676-5680http://hdl.handle.net/11449/696682-s2.0-342483632732-s2.0-34248363273.pdf533325035504981415256654089001950000-0001-7831-1149Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenetics and Molecular Research0,439info:eu-repo/semantics/openAccess2023-12-04T06:12:18Zoai:repositorio.unesp.br:11449/69668Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-04T06:12:18Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Use of a synthetic lethal screen to identify genes related to TIF51A in Saccharomyces cerevisiae |
title |
Use of a synthetic lethal screen to identify genes related to TIF51A in Saccharomyces cerevisiae |
spellingShingle |
Use of a synthetic lethal screen to identify genes related to TIF51A in Saccharomyces cerevisiae Frigieri, M. C. [UNESP] eIF5A Genetic interaction Synthetic lethality Vesicular trafficking Ypt1 guanosine triphosphatase initiation factor 5A Rab protein synthetic DNA allele budding cell proliferation gene identification gene mutation genetic screening lethal gene nonhuman nucleocytoplasmic transport protein secretion protein synthesis Saccharomyces cerevisiae translation initiation G1 Phase Genes, Lethal Mutation Peptide Initiation Factors rab GTP-Binding Proteins RNA-Binding Proteins S Phase Saccharomyces cerevisiae Proteins Transport Vesicles Eukaryota |
title_short |
Use of a synthetic lethal screen to identify genes related to TIF51A in Saccharomyces cerevisiae |
title_full |
Use of a synthetic lethal screen to identify genes related to TIF51A in Saccharomyces cerevisiae |
title_fullStr |
Use of a synthetic lethal screen to identify genes related to TIF51A in Saccharomyces cerevisiae |
title_full_unstemmed |
Use of a synthetic lethal screen to identify genes related to TIF51A in Saccharomyces cerevisiae |
title_sort |
Use of a synthetic lethal screen to identify genes related to TIF51A in Saccharomyces cerevisiae |
author |
Frigieri, M. C. [UNESP] |
author_facet |
Frigieri, M. C. [UNESP] Thompson, G. M. [UNESP] Pandolfi, J. R. [UNESP] Zanelli, Cleslei Fernando [UNESP] Valentini, Sandro Roberto [UNESP] |
author_role |
author |
author2 |
Thompson, G. M. [UNESP] Pandolfi, J. R. [UNESP] Zanelli, Cleslei Fernando [UNESP] Valentini, Sandro Roberto [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Frigieri, M. C. [UNESP] Thompson, G. M. [UNESP] Pandolfi, J. R. [UNESP] Zanelli, Cleslei Fernando [UNESP] Valentini, Sandro Roberto [UNESP] |
dc.subject.por.fl_str_mv |
eIF5A Genetic interaction Synthetic lethality Vesicular trafficking Ypt1 guanosine triphosphatase initiation factor 5A Rab protein synthetic DNA allele budding cell proliferation gene identification gene mutation genetic screening lethal gene nonhuman nucleocytoplasmic transport protein secretion protein synthesis Saccharomyces cerevisiae translation initiation G1 Phase Genes, Lethal Mutation Peptide Initiation Factors rab GTP-Binding Proteins RNA-Binding Proteins S Phase Saccharomyces cerevisiae Proteins Transport Vesicles Eukaryota |
topic |
eIF5A Genetic interaction Synthetic lethality Vesicular trafficking Ypt1 guanosine triphosphatase initiation factor 5A Rab protein synthetic DNA allele budding cell proliferation gene identification gene mutation genetic screening lethal gene nonhuman nucleocytoplasmic transport protein secretion protein synthesis Saccharomyces cerevisiae translation initiation G1 Phase Genes, Lethal Mutation Peptide Initiation Factors rab GTP-Binding Proteins RNA-Binding Proteins S Phase Saccharomyces cerevisiae Proteins Transport Vesicles Eukaryota |
description |
The putative eukaryotic translation initiation factor 5A (eIF5A) is an essential protein for cell viability and the only cellular protein known to contain the unusual amino acid residue hypusine. eIF5A has been implicated in translation initiation, cell proliferation, nucleocytoplasmic transport, mRNA decay, and actin polarization, but the precise biological function of this protein is not clear. However, eIF5A was recently shown to be directly involved with the translational machinery. A screen for synthetic lethal mutations was carried out with one of the temperature-sensitive alleles of TIF51A (tif51A-3) to identify factors that functionally interact with eIF5A and revealed the essential gene YPT1. This gene encodes a small GTPase, a member of the rab family involved with secretion, acting in the vesicular trafficking between endoplasmatic reticulum and the Golgi. Thus, the synthetic lethality between TIF51A and YPT1 may reveal the connection between translation and the polarized distribution of membrane components, suggesting that these proteins work together in the cell to guarantee proper protein synthesis and secretion necessary for correct bud formation during G1/ S transition. Future studies will investigate the functional interaction between eIF5A and Ypt1 in order to clarify this involvement of eIF5A with vesicular trafficking. ©FUNPEC-RP. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-05-18 2014-05-27T11:22:28Z 2014-05-27T11:22:28Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.geneticsmr.com//year2007/vol6-1/pdf/gmr0282.pdf Genetics and Molecular Research, v. 6, n. 1, p. 152-165, 2007. 1676-5680 http://hdl.handle.net/11449/69668 2-s2.0-34248363273 2-s2.0-34248363273.pdf 5333250355049814 1525665408900195 0000-0001-7831-1149 |
url |
http://www.geneticsmr.com//year2007/vol6-1/pdf/gmr0282.pdf http://hdl.handle.net/11449/69668 |
identifier_str_mv |
Genetics and Molecular Research, v. 6, n. 1, p. 152-165, 2007. 1676-5680 2-s2.0-34248363273 2-s2.0-34248363273.pdf 5333250355049814 1525665408900195 0000-0001-7831-1149 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Genetics and Molecular Research 0,439 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
152-165 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1799965166064893952 |