Pseudomonas putida Stimulates Primordia on Agaricus bitorquis
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s00284-015-0982-8 http://hdl.handle.net/11449/158727 |
Resumo: | Casing layer is one step of Agaricus bisporus cultivation where there is a competitive environment with a high number of microorganisms and diversity interacting with mycelia. It is suggested that a minimal community of these microorganisms would be necessary to stimulate fructification. However, A. bisporus is not able to produce primordia in sterile casing layers or Petri dishes. Thus, the objective of this study was to characterize bacterial microbiota of casing layers from A. bisporus cultivation, isolate, identify and characterize the bacteria responsible for the stimulation of primordium and their action mechanism using Agaricus bitorquis as a primordium stimulation model. Bacterial and Pseudomonas spp. communities of different casing layers of A. bisporus cultivation were collected and quantified. It was concluded that Pseudomonas spp. corresponds to 75-85 % of bacterial population of the casing layers in A. bisporus cultivation and among those 12 % are Pseudomonas putida. Four biochemical assays were used to identify P. putida. In vitro primordium stimulation of living P. putida and non-living bacterial suspensions, after chemical or physical treatments, was tested using A. bitorquis as a primordium stimulation model. Primordium stimulation assay was registered by photographs, and micrographs of vertical cut of primordium were registered by scanning electron microscope. Interaction of living P. putida with A. bitorquis mycelia is capable of stimulating primordial instead of non-living bacterial suspensions. Stimulation of A. bitorquis primordia does not imply or is related to mycelial growth inhibition, but a hierarchical relation of primordium succession and development is suggested. |
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Pseudomonas putida Stimulates Primordia on Agaricus bitorquisCasing layer is one step of Agaricus bisporus cultivation where there is a competitive environment with a high number of microorganisms and diversity interacting with mycelia. It is suggested that a minimal community of these microorganisms would be necessary to stimulate fructification. However, A. bisporus is not able to produce primordia in sterile casing layers or Petri dishes. Thus, the objective of this study was to characterize bacterial microbiota of casing layers from A. bisporus cultivation, isolate, identify and characterize the bacteria responsible for the stimulation of primordium and their action mechanism using Agaricus bitorquis as a primordium stimulation model. Bacterial and Pseudomonas spp. communities of different casing layers of A. bisporus cultivation were collected and quantified. It was concluded that Pseudomonas spp. corresponds to 75-85 % of bacterial population of the casing layers in A. bisporus cultivation and among those 12 % are Pseudomonas putida. Four biochemical assays were used to identify P. putida. In vitro primordium stimulation of living P. putida and non-living bacterial suspensions, after chemical or physical treatments, was tested using A. bitorquis as a primordium stimulation model. Primordium stimulation assay was registered by photographs, and micrographs of vertical cut of primordium were registered by scanning electron microscope. Interaction of living P. putida with A. bitorquis mycelia is capable of stimulating primordial instead of non-living bacterial suspensions. Stimulation of A. bitorquis primordia does not imply or is related to mycelial growth inhibition, but a hierarchical relation of primordium succession and development is suggested.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Paulista State University (UNESP)Horticulture Research InternationalUniv Paranaense, Postgrad Program Biotechnol Appl Agr, Umuarama, PR, BrazilHort Res Int, Wellesbourne CV35 9EF, Warwick, EnglandUniv Estadual Paulista, Mushroom Prod, Botucatu, SP, BrazilUniv Estadual Paulista, Mushroom Prod, Botucatu, SP, BrazilSpringerUniv ParanaenseHort Res IntUniversidade Estadual Paulista (Unesp)Colauto, Nelson B.Fermor, Terry R.Eira, Augusto F. [UNESP]Linde, Giani A.2018-11-26T15:28:47Z2018-11-26T15:28:47Z2016-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article482-488application/pdfhttp://dx.doi.org/10.1007/s00284-015-0982-8Current Microbiology. New York: Springer, v. 72, n. 4, p. 482-488, 2016.0343-8651http://hdl.handle.net/11449/15872710.1007/s00284-015-0982-8WOS:000371250500017WOS:000371250500017.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCurrent Microbiology0,562info:eu-repo/semantics/openAccess2024-01-25T06:30:06Zoai:repositorio.unesp.br:11449/158727Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:54:39.642912Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Pseudomonas putida Stimulates Primordia on Agaricus bitorquis |
title |
Pseudomonas putida Stimulates Primordia on Agaricus bitorquis |
spellingShingle |
Pseudomonas putida Stimulates Primordia on Agaricus bitorquis Colauto, Nelson B. |
title_short |
Pseudomonas putida Stimulates Primordia on Agaricus bitorquis |
title_full |
Pseudomonas putida Stimulates Primordia on Agaricus bitorquis |
title_fullStr |
Pseudomonas putida Stimulates Primordia on Agaricus bitorquis |
title_full_unstemmed |
Pseudomonas putida Stimulates Primordia on Agaricus bitorquis |
title_sort |
Pseudomonas putida Stimulates Primordia on Agaricus bitorquis |
author |
Colauto, Nelson B. |
author_facet |
Colauto, Nelson B. Fermor, Terry R. Eira, Augusto F. [UNESP] Linde, Giani A. |
author_role |
author |
author2 |
Fermor, Terry R. Eira, Augusto F. [UNESP] Linde, Giani A. |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Univ Paranaense Hort Res Int Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Colauto, Nelson B. Fermor, Terry R. Eira, Augusto F. [UNESP] Linde, Giani A. |
description |
Casing layer is one step of Agaricus bisporus cultivation where there is a competitive environment with a high number of microorganisms and diversity interacting with mycelia. It is suggested that a minimal community of these microorganisms would be necessary to stimulate fructification. However, A. bisporus is not able to produce primordia in sterile casing layers or Petri dishes. Thus, the objective of this study was to characterize bacterial microbiota of casing layers from A. bisporus cultivation, isolate, identify and characterize the bacteria responsible for the stimulation of primordium and their action mechanism using Agaricus bitorquis as a primordium stimulation model. Bacterial and Pseudomonas spp. communities of different casing layers of A. bisporus cultivation were collected and quantified. It was concluded that Pseudomonas spp. corresponds to 75-85 % of bacterial population of the casing layers in A. bisporus cultivation and among those 12 % are Pseudomonas putida. Four biochemical assays were used to identify P. putida. In vitro primordium stimulation of living P. putida and non-living bacterial suspensions, after chemical or physical treatments, was tested using A. bitorquis as a primordium stimulation model. Primordium stimulation assay was registered by photographs, and micrographs of vertical cut of primordium were registered by scanning electron microscope. Interaction of living P. putida with A. bitorquis mycelia is capable of stimulating primordial instead of non-living bacterial suspensions. Stimulation of A. bitorquis primordia does not imply or is related to mycelial growth inhibition, but a hierarchical relation of primordium succession and development is suggested. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-04-01 2018-11-26T15:28:47Z 2018-11-26T15:28:47Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s00284-015-0982-8 Current Microbiology. New York: Springer, v. 72, n. 4, p. 482-488, 2016. 0343-8651 http://hdl.handle.net/11449/158727 10.1007/s00284-015-0982-8 WOS:000371250500017 WOS:000371250500017.pdf |
url |
http://dx.doi.org/10.1007/s00284-015-0982-8 http://hdl.handle.net/11449/158727 |
identifier_str_mv |
Current Microbiology. New York: Springer, v. 72, n. 4, p. 482-488, 2016. 0343-8651 10.1007/s00284-015-0982-8 WOS:000371250500017 WOS:000371250500017.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Current Microbiology 0,562 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
482-488 application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129563654356992 |