Molecular and serological detection of Babesia Bovis and Babesia Bigemina in cattle in the Rio de Janeiro, Brazil

Detalhes bibliográficos
Autor(a) principal: Silva, Jenevaldo Barbosa da [UNESP]
Data de Publicação: 2014
Outros Autores: Santos, Priscilla Nunes dos, Fonseca, Adivaldo Henrique
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://www.uel.br/revistas/uel/index.php/semagrarias/article/view/15945
http://hdl.handle.net/11449/129634
Resumo: Bovine babesiosis is an important disease of cattle where Rhipicephalus microplus acts as a vector for the two causal organisms Babesia bovis and Babesia bigemina. A total of 22 calves were randomly monitored during three years by semi-nested PCR assay and ELISA test to determine prevalence of B. bovis and B. bigemina. The overall prevalence of B. bovis and B. bigemina was 30% and 35% by nested PCR (nPCR), and 70% and 75% by ELISA, respectively. Statistical analysis of the characteristics of animals showed that age and tick infestations (p<0.05) might play an important role in the spread of babesiosis as animal less than 6 months old. A high correlation (Kappa index of 0.70 for B. bovis and 0.65 for B. bigemina, respectively) between serological and molecular tests suggests that the combination of the utilized techniques in the present study is suitable for babesiosis diagnosis in an endemic unstable area.
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spelling Molecular and serological detection of Babesia Bovis and Babesia Bigemina in cattle in the Rio de Janeiro, BrazilDetecção molecular e sorológica de Babesia Bovis e Babesia Bigemina em bovinos no Rio de Janeiro, BrasilBabesiosisBrazildiagnosisELISAPCRBovine babesiosis is an important disease of cattle where Rhipicephalus microplus acts as a vector for the two causal organisms Babesia bovis and Babesia bigemina. A total of 22 calves were randomly monitored during three years by semi-nested PCR assay and ELISA test to determine prevalence of B. bovis and B. bigemina. The overall prevalence of B. bovis and B. bigemina was 30% and 35% by nested PCR (nPCR), and 70% and 75% by ELISA, respectively. Statistical analysis of the characteristics of animals showed that age and tick infestations (p<0.05) might play an important role in the spread of babesiosis as animal less than 6 months old. A high correlation (Kappa index of 0.70 for B. bovis and 0.65 for B. bigemina, respectively) between serological and molecular tests suggests that the combination of the utilized techniques in the present study is suitable for babesiosis diagnosis in an endemic unstable area.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Universidade Federal Rural Rio de Janeiro, UFRRJ, Seropedica, RJ, BrazilUFRRJ, Seropédica, RJ, BrazilUniversidade Estadual Paulista, FCAV UNESP, Fac Ciencias Agr &Vet, Jaboticabal, SP, BrazilUniv Estadual LondrinaUniversidade Estadual Paulista (Unesp)Universidade Federal Rural Rio de Janeiro (UFRRJ)Silva, Jenevaldo Barbosa da [UNESP]Santos, Priscilla Nunes dosFonseca, Adivaldo Henrique2015-10-22T06:20:23Z2015-10-22T06:20:23Z2014info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article3139-3145application/pdfhttp://www.uel.br/revistas/uel/index.php/semagrarias/article/view/15945Semina-ciencias Agrarias. Londrina: Univ Estadual Londrina, v. 35, n. 6, p. 3139-3145, 2014.1676-546Xhttp://hdl.handle.net/11449/12963410.5433/1679-0359.2014v35n6p3139WOS:000352022600022WOS000352022600022.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengSemina-ciencias Agrárias0.349info:eu-repo/semantics/openAccess2023-10-17T06:04:08Zoai:repositorio.unesp.br:11449/129634Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-17T06:04:08Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Molecular and serological detection of Babesia Bovis and Babesia Bigemina in cattle in the Rio de Janeiro, Brazil
Detecção molecular e sorológica de Babesia Bovis e Babesia Bigemina em bovinos no Rio de Janeiro, Brasil
title Molecular and serological detection of Babesia Bovis and Babesia Bigemina in cattle in the Rio de Janeiro, Brazil
spellingShingle Molecular and serological detection of Babesia Bovis and Babesia Bigemina in cattle in the Rio de Janeiro, Brazil
Silva, Jenevaldo Barbosa da [UNESP]
Babesiosis
Brazil
diagnosis
ELISA
PCR
title_short Molecular and serological detection of Babesia Bovis and Babesia Bigemina in cattle in the Rio de Janeiro, Brazil
title_full Molecular and serological detection of Babesia Bovis and Babesia Bigemina in cattle in the Rio de Janeiro, Brazil
title_fullStr Molecular and serological detection of Babesia Bovis and Babesia Bigemina in cattle in the Rio de Janeiro, Brazil
title_full_unstemmed Molecular and serological detection of Babesia Bovis and Babesia Bigemina in cattle in the Rio de Janeiro, Brazil
title_sort Molecular and serological detection of Babesia Bovis and Babesia Bigemina in cattle in the Rio de Janeiro, Brazil
author Silva, Jenevaldo Barbosa da [UNESP]
author_facet Silva, Jenevaldo Barbosa da [UNESP]
Santos, Priscilla Nunes dos
Fonseca, Adivaldo Henrique
author_role author
author2 Santos, Priscilla Nunes dos
Fonseca, Adivaldo Henrique
author2_role author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade Federal Rural Rio de Janeiro (UFRRJ)
dc.contributor.author.fl_str_mv Silva, Jenevaldo Barbosa da [UNESP]
Santos, Priscilla Nunes dos
Fonseca, Adivaldo Henrique
dc.subject.por.fl_str_mv Babesiosis
Brazil
diagnosis
ELISA
PCR
topic Babesiosis
Brazil
diagnosis
ELISA
PCR
description Bovine babesiosis is an important disease of cattle where Rhipicephalus microplus acts as a vector for the two causal organisms Babesia bovis and Babesia bigemina. A total of 22 calves were randomly monitored during three years by semi-nested PCR assay and ELISA test to determine prevalence of B. bovis and B. bigemina. The overall prevalence of B. bovis and B. bigemina was 30% and 35% by nested PCR (nPCR), and 70% and 75% by ELISA, respectively. Statistical analysis of the characteristics of animals showed that age and tick infestations (p<0.05) might play an important role in the spread of babesiosis as animal less than 6 months old. A high correlation (Kappa index of 0.70 for B. bovis and 0.65 for B. bigemina, respectively) between serological and molecular tests suggests that the combination of the utilized techniques in the present study is suitable for babesiosis diagnosis in an endemic unstable area.
publishDate 2014
dc.date.none.fl_str_mv 2014
2015-10-22T06:20:23Z
2015-10-22T06:20:23Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.uel.br/revistas/uel/index.php/semagrarias/article/view/15945
Semina-ciencias Agrarias. Londrina: Univ Estadual Londrina, v. 35, n. 6, p. 3139-3145, 2014.
1676-546X
http://hdl.handle.net/11449/129634
10.5433/1679-0359.2014v35n6p3139
WOS:000352022600022
WOS000352022600022.pdf
url http://www.uel.br/revistas/uel/index.php/semagrarias/article/view/15945
http://hdl.handle.net/11449/129634
identifier_str_mv Semina-ciencias Agrarias. Londrina: Univ Estadual Londrina, v. 35, n. 6, p. 3139-3145, 2014.
1676-546X
10.5433/1679-0359.2014v35n6p3139
WOS:000352022600022
WOS000352022600022.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Semina-ciencias Agrárias
0.349
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 3139-3145
application/pdf
dc.publisher.none.fl_str_mv Univ Estadual Londrina
publisher.none.fl_str_mv Univ Estadual Londrina
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1799964591968485376

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