Effects of excessive energy intake and supplementation with chromium propionate on insulin resistance parameters, milk production, and reproductive outcomes of lactating dairy cows
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.livsci.2015.08.007 http://hdl.handle.net/11449/168021 |
Resumo: | This experiment compared insulin resistance parameters, milk production, and reproductive outcomes in: (1) lactating dairy cows consuming adequate or excessive energy, and (2) lactating dairy cows consuming excessive energy and receiving or not Cr-propionate supplementation. Seventeen primiparous and multiparous, non-pregnant, lactating Holstein cows (initial days in milk=76±2) were assigned on d 0 to: (1) concentrate intake to meet their requirements of net energy for lactation (NE<inf>L</inf>) without Cr supplementation (MAN; n=5), (2) concentrate intake to exceed their NE<inf>L</inf> requirements without Cr supplementation (HIGH; n=6), and (3) HIGH with 2.5g/d of Cr propionate (HIGHCR; n=6). Throughout the experiment (d 0 to 210), cows were offered corn silage for ad libitum consumption, and individually received a corn-based concentrate twice daily. Concentrate intake was formulated to allow diets to provide 100% of daily NE<inf>L</inf> requirements of MAN, and 160% of daily NE<inf>L</inf> requirements of HIGH and HIGHCR cows. Cow BW, BCS, and milk production were recorded weekly. Blood samples were collected weekly, prior to and at 2 and 4h after the morning concentrate feeding. Six glucose tolerance tests (GTT) were performed, every 42d, by infusing cows with 0.5g of glucose/kg of BW. Follicle aspiration for in vitro embryo production was performed via transvaginal ovum pick-up 2d after each GTT. Increase in BCS from d 0 to 210 was greater (P≤0.04) in HIGH and HIGHCR vs. MAN. Milk production was similar (P=0.92) between treatments. Within weekly samples, serum non-esterified fatty acids concentration were greater (P≤0.05) for MAN vs. HIGH and HIGHCR. Serum insulin concentrations and insulin:glucose ratio were often greater (P≤0.05) for HIGH, intermediate for HIGHCR, and lesser for MAN (treatment×day interaction, P<0.01). During the GTT, serum insulin concentrations and insulin:glucose ratio were greater (P≤0.05) for HIGH compared with HIGHCR and MAN from 10 to 60min relative to the time of glucose infusion. Proportion of embryo produced per oocyte collected was greater (P≤0.02) for MAN vs. HIGH and HIGHCR, and similar (P=0.59) between HIGH and HIGHCR. In conclusion, lactating cows consuming excessive concentrate and NE<inf>L</inf> experienced increased insulin resistance and reduced proportion of embryo produced per oocyte collected compared with cows consuming adequate amounts of energy, whereas Cr-propionate supplementation was effective in alleviating insulin resistance caused by excessive NE<inf>L</inf> intake. |
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Effects of excessive energy intake and supplementation with chromium propionate on insulin resistance parameters, milk production, and reproductive outcomes of lactating dairy cowsChromiumDairy cowsEnergy intakeInsulin resistanceThis experiment compared insulin resistance parameters, milk production, and reproductive outcomes in: (1) lactating dairy cows consuming adequate or excessive energy, and (2) lactating dairy cows consuming excessive energy and receiving or not Cr-propionate supplementation. Seventeen primiparous and multiparous, non-pregnant, lactating Holstein cows (initial days in milk=76±2) were assigned on d 0 to: (1) concentrate intake to meet their requirements of net energy for lactation (NE<inf>L</inf>) without Cr supplementation (MAN; n=5), (2) concentrate intake to exceed their NE<inf>L</inf> requirements without Cr supplementation (HIGH; n=6), and (3) HIGH with 2.5g/d of Cr propionate (HIGHCR; n=6). Throughout the experiment (d 0 to 210), cows were offered corn silage for ad libitum consumption, and individually received a corn-based concentrate twice daily. Concentrate intake was formulated to allow diets to provide 100% of daily NE<inf>L</inf> requirements of MAN, and 160% of daily NE<inf>L</inf> requirements of HIGH and HIGHCR cows. Cow BW, BCS, and milk production were recorded weekly. Blood samples were collected weekly, prior to and at 2 and 4h after the morning concentrate feeding. Six glucose tolerance tests (GTT) were performed, every 42d, by infusing cows with 0.5g of glucose/kg of BW. Follicle aspiration for in vitro embryo production was performed via transvaginal ovum pick-up 2d after each GTT. Increase in BCS from d 0 to 210 was greater (P≤0.04) in HIGH and HIGHCR vs. MAN. Milk production was similar (P=0.92) between treatments. Within weekly samples, serum non-esterified fatty acids concentration were greater (P≤0.05) for MAN vs. HIGH and HIGHCR. Serum insulin concentrations and insulin:glucose ratio were often greater (P≤0.05) for HIGH, intermediate for HIGHCR, and lesser for MAN (treatment×day interaction, P<0.01). During the GTT, serum insulin concentrations and insulin:glucose ratio were greater (P≤0.05) for HIGH compared with HIGHCR and MAN from 10 to 60min relative to the time of glucose infusion. Proportion of embryo produced per oocyte collected was greater (P≤0.02) for MAN vs. HIGH and HIGHCR, and similar (P=0.59) between HIGH and HIGHCR. In conclusion, lactating cows consuming excessive concentrate and NE<inf>L</inf> experienced increased insulin resistance and reduced proportion of embryo produced per oocyte collected compared with cows consuming adequate amounts of energy, whereas Cr-propionate supplementation was effective in alleviating insulin resistance caused by excessive NE<inf>L</inf> intake.São Paulo State University, Department of Animal ProductionOregon State University, Eastern Oregon Agricultural Research CenterFaculdade de Medicina Veterinária e Zootecnia, UNESP - Univ. Estadual PaulistaSão Paulo State University, Department of Animal ProductionFaculdade de Medicina Veterinária e Zootecnia, UNESP - Univ. Estadual PaulistaUniversidade Estadual Paulista (Unesp)Oregon State University, Eastern Oregon Agricultural Research CenterLeiva, T. [UNESP]Cooke, R. F. [UNESP]Brandão, A. P. [UNESP]Aboin, A. C. [UNESP]Ranches, J. [UNESP]Vasconcelos, J. L.M. [UNESP]2018-12-11T16:39:16Z2018-12-11T16:39:16Z2015-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article121-128application/pdfhttp://dx.doi.org/10.1016/j.livsci.2015.08.007Livestock Science, v. 180, p. 121-128.1871-1413http://hdl.handle.net/11449/16802110.1016/j.livsci.2015.08.0072-s2.0-849419355212-s2.0-84941935521.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengLivestock Science0,730info:eu-repo/semantics/openAccess2023-10-21T06:08:40Zoai:repositorio.unesp.br:11449/168021Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:32:04.020354Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effects of excessive energy intake and supplementation with chromium propionate on insulin resistance parameters, milk production, and reproductive outcomes of lactating dairy cows |
title |
Effects of excessive energy intake and supplementation with chromium propionate on insulin resistance parameters, milk production, and reproductive outcomes of lactating dairy cows |
spellingShingle |
Effects of excessive energy intake and supplementation with chromium propionate on insulin resistance parameters, milk production, and reproductive outcomes of lactating dairy cows Leiva, T. [UNESP] Chromium Dairy cows Energy intake Insulin resistance |
title_short |
Effects of excessive energy intake and supplementation with chromium propionate on insulin resistance parameters, milk production, and reproductive outcomes of lactating dairy cows |
title_full |
Effects of excessive energy intake and supplementation with chromium propionate on insulin resistance parameters, milk production, and reproductive outcomes of lactating dairy cows |
title_fullStr |
Effects of excessive energy intake and supplementation with chromium propionate on insulin resistance parameters, milk production, and reproductive outcomes of lactating dairy cows |
title_full_unstemmed |
Effects of excessive energy intake and supplementation with chromium propionate on insulin resistance parameters, milk production, and reproductive outcomes of lactating dairy cows |
title_sort |
Effects of excessive energy intake and supplementation with chromium propionate on insulin resistance parameters, milk production, and reproductive outcomes of lactating dairy cows |
author |
Leiva, T. [UNESP] |
author_facet |
Leiva, T. [UNESP] Cooke, R. F. [UNESP] Brandão, A. P. [UNESP] Aboin, A. C. [UNESP] Ranches, J. [UNESP] Vasconcelos, J. L.M. [UNESP] |
author_role |
author |
author2 |
Cooke, R. F. [UNESP] Brandão, A. P. [UNESP] Aboin, A. C. [UNESP] Ranches, J. [UNESP] Vasconcelos, J. L.M. [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Oregon State University, Eastern Oregon Agricultural Research Center |
dc.contributor.author.fl_str_mv |
Leiva, T. [UNESP] Cooke, R. F. [UNESP] Brandão, A. P. [UNESP] Aboin, A. C. [UNESP] Ranches, J. [UNESP] Vasconcelos, J. L.M. [UNESP] |
dc.subject.por.fl_str_mv |
Chromium Dairy cows Energy intake Insulin resistance |
topic |
Chromium Dairy cows Energy intake Insulin resistance |
description |
This experiment compared insulin resistance parameters, milk production, and reproductive outcomes in: (1) lactating dairy cows consuming adequate or excessive energy, and (2) lactating dairy cows consuming excessive energy and receiving or not Cr-propionate supplementation. Seventeen primiparous and multiparous, non-pregnant, lactating Holstein cows (initial days in milk=76±2) were assigned on d 0 to: (1) concentrate intake to meet their requirements of net energy for lactation (NE<inf>L</inf>) without Cr supplementation (MAN; n=5), (2) concentrate intake to exceed their NE<inf>L</inf> requirements without Cr supplementation (HIGH; n=6), and (3) HIGH with 2.5g/d of Cr propionate (HIGHCR; n=6). Throughout the experiment (d 0 to 210), cows were offered corn silage for ad libitum consumption, and individually received a corn-based concentrate twice daily. Concentrate intake was formulated to allow diets to provide 100% of daily NE<inf>L</inf> requirements of MAN, and 160% of daily NE<inf>L</inf> requirements of HIGH and HIGHCR cows. Cow BW, BCS, and milk production were recorded weekly. Blood samples were collected weekly, prior to and at 2 and 4h after the morning concentrate feeding. Six glucose tolerance tests (GTT) were performed, every 42d, by infusing cows with 0.5g of glucose/kg of BW. Follicle aspiration for in vitro embryo production was performed via transvaginal ovum pick-up 2d after each GTT. Increase in BCS from d 0 to 210 was greater (P≤0.04) in HIGH and HIGHCR vs. MAN. Milk production was similar (P=0.92) between treatments. Within weekly samples, serum non-esterified fatty acids concentration were greater (P≤0.05) for MAN vs. HIGH and HIGHCR. Serum insulin concentrations and insulin:glucose ratio were often greater (P≤0.05) for HIGH, intermediate for HIGHCR, and lesser for MAN (treatment×day interaction, P<0.01). During the GTT, serum insulin concentrations and insulin:glucose ratio were greater (P≤0.05) for HIGH compared with HIGHCR and MAN from 10 to 60min relative to the time of glucose infusion. Proportion of embryo produced per oocyte collected was greater (P≤0.02) for MAN vs. HIGH and HIGHCR, and similar (P=0.59) between HIGH and HIGHCR. In conclusion, lactating cows consuming excessive concentrate and NE<inf>L</inf> experienced increased insulin resistance and reduced proportion of embryo produced per oocyte collected compared with cows consuming adequate amounts of energy, whereas Cr-propionate supplementation was effective in alleviating insulin resistance caused by excessive NE<inf>L</inf> intake. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-01-01 2018-12-11T16:39:16Z 2018-12-11T16:39:16Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.livsci.2015.08.007 Livestock Science, v. 180, p. 121-128. 1871-1413 http://hdl.handle.net/11449/168021 10.1016/j.livsci.2015.08.007 2-s2.0-84941935521 2-s2.0-84941935521.pdf |
url |
http://dx.doi.org/10.1016/j.livsci.2015.08.007 http://hdl.handle.net/11449/168021 |
identifier_str_mv |
Livestock Science, v. 180, p. 121-128. 1871-1413 10.1016/j.livsci.2015.08.007 2-s2.0-84941935521 2-s2.0-84941935521.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Livestock Science 0,730 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
121-128 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128528716136448 |