Fractal dimension analysis: A new tool for analyzing colony-forming units
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.mex.2021.101228 http://hdl.handle.net/11449/207144 |
Resumo: | The gold standard for quantifying bacteria both in routine diagnostics and in research is plating followed by count of colony-forming units (CFU). But, manual CFU counting on plates is time-consuming and subjective. We evaluated fractal dimension as a new methodology for evaluating CFU. Twenty fragments of expanded polytetrafluoroethylene (ePTFE) synthetic vascular prosthesis and 20 silicone prostheses were embedded in bacterial suspensions and incubated. The prostheses were then sown in solid culture medium and incubated for 48 h. Petri dishes were photographed and analyzed by fractal dimension. There was correlation between the number of CFU in manual counting and the fractal dimension analysis (p = 0.0001). We demonstrated that fractal dimension is a useful method for microbiological analyses in researches. It makes CFU analysis easier and faster and can be used regardless of the culture medium. • Petri dishes with different bacterial colonies were photographed with a digital camera under natural light. • The images were binarized and analyzed with ImageJⓇ's “fractal dimension” tool. • Fractal dimension analysis showed to be a good tool for evaluating the amount of colony-forming unit. |
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Fractal dimension analysis: A new tool for analyzing colony-forming unitsColony-forming units counting (CFU)Computer-assistedImage processingMicrobial colony countProstheses and implantsThe gold standard for quantifying bacteria both in routine diagnostics and in research is plating followed by count of colony-forming units (CFU). But, manual CFU counting on plates is time-consuming and subjective. We evaluated fractal dimension as a new methodology for evaluating CFU. Twenty fragments of expanded polytetrafluoroethylene (ePTFE) synthetic vascular prosthesis and 20 silicone prostheses were embedded in bacterial suspensions and incubated. The prostheses were then sown in solid culture medium and incubated for 48 h. Petri dishes were photographed and analyzed by fractal dimension. There was correlation between the number of CFU in manual counting and the fractal dimension analysis (p = 0.0001). We demonstrated that fractal dimension is a useful method for microbiological analyses in researches. It makes CFU analysis easier and faster and can be used regardless of the culture medium. • Petri dishes with different bacterial colonies were photographed with a digital camera under natural light. • The images were binarized and analyzed with ImageJⓇ's “fractal dimension” tool. • Fractal dimension analysis showed to be a good tool for evaluating the amount of colony-forming unit.Department of Pathology Universidade do Oeste Paulista (UNOESTE), Rua José Bongiovani, 700Department of Surgery Universidade do Oeste Paulista (UNOESTE), Rua José Bongiovani, 700Laboratory of Clinical Analysis Universidade do Oeste Paulista (UNOESTE), Rua José Bongiovani, 700Medical College Universidade do Oeste Paulista (UNOESTE), Rua José Bongiovani, 700Department of Microbiology Graduate Program in Health Sciences Universidade do Oeste Paulista (UNOESTE), Rua José Bongiovani, 700Department of Pathology Botucatu Medical School São Paulo State University (UNESP), Rubião Junior, s/n, 18618-970Department of Pathology Botucatu Medical School São Paulo State University (UNESP), Rubião Junior, s/n, 18618-970Universidade do Oeste Paulista (UNOESTE)Universidade Estadual Paulista (Unesp)Nai, Gisele AlborghettiMartelli, Cesar Alberto TALAveraMedina, Denis Aloísio LopesOliveira, Mayla Silva Cayres deCaldeira, Isadora DelfinoHenriques, Bruno CarvalhoPortelinha, Maria Júlia SchadeckEller, Lizziane Kretli WinkelstroterMarques, Mariângela Esther Alencar [UNESP]2021-06-25T10:49:37Z2021-06-25T10:49:37Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.mex.2021.101228MethodsX, v. 8.2215-0161http://hdl.handle.net/11449/20714410.1016/j.mex.2021.1012282-s2.0-85099395121Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMethodsXinfo:eu-repo/semantics/openAccess2021-10-23T16:16:15Zoai:repositorio.unesp.br:11449/207144Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T16:16:15Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Fractal dimension analysis: A new tool for analyzing colony-forming units |
title |
Fractal dimension analysis: A new tool for analyzing colony-forming units |
spellingShingle |
Fractal dimension analysis: A new tool for analyzing colony-forming units Nai, Gisele Alborghetti Colony-forming units counting (CFU) Computer-assisted Image processing Microbial colony count Prostheses and implants |
title_short |
Fractal dimension analysis: A new tool for analyzing colony-forming units |
title_full |
Fractal dimension analysis: A new tool for analyzing colony-forming units |
title_fullStr |
Fractal dimension analysis: A new tool for analyzing colony-forming units |
title_full_unstemmed |
Fractal dimension analysis: A new tool for analyzing colony-forming units |
title_sort |
Fractal dimension analysis: A new tool for analyzing colony-forming units |
author |
Nai, Gisele Alborghetti |
author_facet |
Nai, Gisele Alborghetti Martelli, Cesar Alberto TALAvera Medina, Denis Aloísio Lopes Oliveira, Mayla Silva Cayres de Caldeira, Isadora Delfino Henriques, Bruno Carvalho Portelinha, Maria Júlia Schadeck Eller, Lizziane Kretli Winkelstroter Marques, Mariângela Esther Alencar [UNESP] |
author_role |
author |
author2 |
Martelli, Cesar Alberto TALAvera Medina, Denis Aloísio Lopes Oliveira, Mayla Silva Cayres de Caldeira, Isadora Delfino Henriques, Bruno Carvalho Portelinha, Maria Júlia Schadeck Eller, Lizziane Kretli Winkelstroter Marques, Mariângela Esther Alencar [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Oeste Paulista (UNOESTE) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Nai, Gisele Alborghetti Martelli, Cesar Alberto TALAvera Medina, Denis Aloísio Lopes Oliveira, Mayla Silva Cayres de Caldeira, Isadora Delfino Henriques, Bruno Carvalho Portelinha, Maria Júlia Schadeck Eller, Lizziane Kretli Winkelstroter Marques, Mariângela Esther Alencar [UNESP] |
dc.subject.por.fl_str_mv |
Colony-forming units counting (CFU) Computer-assisted Image processing Microbial colony count Prostheses and implants |
topic |
Colony-forming units counting (CFU) Computer-assisted Image processing Microbial colony count Prostheses and implants |
description |
The gold standard for quantifying bacteria both in routine diagnostics and in research is plating followed by count of colony-forming units (CFU). But, manual CFU counting on plates is time-consuming and subjective. We evaluated fractal dimension as a new methodology for evaluating CFU. Twenty fragments of expanded polytetrafluoroethylene (ePTFE) synthetic vascular prosthesis and 20 silicone prostheses were embedded in bacterial suspensions and incubated. The prostheses were then sown in solid culture medium and incubated for 48 h. Petri dishes were photographed and analyzed by fractal dimension. There was correlation between the number of CFU in manual counting and the fractal dimension analysis (p = 0.0001). We demonstrated that fractal dimension is a useful method for microbiological analyses in researches. It makes CFU analysis easier and faster and can be used regardless of the culture medium. • Petri dishes with different bacterial colonies were photographed with a digital camera under natural light. • The images were binarized and analyzed with ImageJⓇ's “fractal dimension” tool. • Fractal dimension analysis showed to be a good tool for evaluating the amount of colony-forming unit. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T10:49:37Z 2021-06-25T10:49:37Z 2021-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.mex.2021.101228 MethodsX, v. 8. 2215-0161 http://hdl.handle.net/11449/207144 10.1016/j.mex.2021.101228 2-s2.0-85099395121 |
url |
http://dx.doi.org/10.1016/j.mex.2021.101228 http://hdl.handle.net/11449/207144 |
identifier_str_mv |
MethodsX, v. 8. 2215-0161 10.1016/j.mex.2021.101228 2-s2.0-85099395121 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
MethodsX |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965523016941568 |