Trypanocidal action of eupomatenoid-5 is related to mitochondrion dysfunction and oxidative damage in Trypanosoma cruzi
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.micinf.2011.05.011 http://hdl.handle.net/11449/8789 |
Resumo: | Because of its severe side effects and variable efficacy, the current treatment for Chagas disease is unsatisfactory. Natural compounds are good alternative chemotherapeutic agents for the treatment of this infection. Recently, our group reported the antiproliferative activity and morphological alterations in epimastigotes and intracellular amastigotes of Trypanosoma cruzi treated with eupomatenoid-5, a neolignan isolated from leaves of Piper regnellii var. pallescens. Here, we demonstrate that eupomatenoid-5 exhibited activity against trypomastigotes, the infective form of T cruzi (EC(50) 40.5 mu W), leading to ultrastructural alteration and lipoperoxidation in the cell membrane. Additionally, eupomatenoid-5 induced depolarization of the mitochondrial membrane, lipoperoxidation and increased G6PD activity in epimastigotes of T cruzi. These findings support the possibility that different mechanisms may be targeted, according to the form of the parasite, and that the plasma membrane and mitochondria are the structures that are most affected in trypomastigotes and epimastigotes, respectively. Thus, the trypanocidal action of eupomatenoid-5 may be associated with mitochondrial dysfunction and oxidative damage, which can trigger destructive effects on biological molecules of T cruzi, leading to parasite death. (C) 2011 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved. |
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Trypanocidal action of eupomatenoid-5 is related to mitochondrion dysfunction and oxidative damage in Trypanosoma cruziTrypanosoma cruziEupomatenoid-5Antioxidant systemBecause of its severe side effects and variable efficacy, the current treatment for Chagas disease is unsatisfactory. Natural compounds are good alternative chemotherapeutic agents for the treatment of this infection. Recently, our group reported the antiproliferative activity and morphological alterations in epimastigotes and intracellular amastigotes of Trypanosoma cruzi treated with eupomatenoid-5, a neolignan isolated from leaves of Piper regnellii var. pallescens. Here, we demonstrate that eupomatenoid-5 exhibited activity against trypomastigotes, the infective form of T cruzi (EC(50) 40.5 mu W), leading to ultrastructural alteration and lipoperoxidation in the cell membrane. Additionally, eupomatenoid-5 induced depolarization of the mitochondrial membrane, lipoperoxidation and increased G6PD activity in epimastigotes of T cruzi. These findings support the possibility that different mechanisms may be targeted, according to the form of the parasite, and that the plasma membrane and mitochondria are the structures that are most affected in trypomastigotes and epimastigotes, respectively. Thus, the trypanocidal action of eupomatenoid-5 may be associated with mitochondrial dysfunction and oxidative damage, which can trigger destructive effects on biological molecules of T cruzi, leading to parasite death. (C) 2011 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Financiadora de Estudos e Projetos (FINEP)Fundação Araucária de Apoio ao Desenvolvimento Científico e Tecnológico do Paraná (FAADCT/PR)Pos-graduacao em Microbiologia da Universidade Estadual de Londrina (UEL)Universidade Estadual de Londrina (UEL), Programa Posgrad Microbiol, Londrina, Pr, BrazilUniversidade Estadual de Maringá (UEM), Dept Ciencias Basicas Saude, Maringa, Pr, BrazilUnesp Univ Estadual Paulista, Fac Ciencias, Dept Quim, Bauru, SP, BrazilUniversidade Estadual de Maringá (UEM), Lab Inovacao Tecnol Desenvolvimento Farmacos & Co, Programa Posgrad Ciencias Farmaceut, Maringa, Pr, BrazilUnesp Univ Estadual Paulista, Fac Ciencias, Dept Quim, Bauru, SP, BrazilElsevier B.V.Universidade Estadual de Londrina (UEL)Universidade Estadual de Maringá (UEM)Universidade Estadual Paulista (Unesp)Pelizzaro-Rocha, Karin JulianeVeiga-Santos, PhercylesLazarin-Bidoia, DanielleUeda-Nakamura, TaniaDias Filho, Benedito PradoXimenes, Valdecir Farias [UNESP]Silva, Sueli OliveiraNakamura, Celso Vataru2014-05-20T13:27:00Z2014-05-20T13:27:00Z2011-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1018-1024application/pdfhttp://dx.doi.org/10.1016/j.micinf.2011.05.011Microbes and Infection. Amsterdam: Elsevier B.V., v. 13, n. 12-13, p. 1018-1024, 2011.1286-4579http://hdl.handle.net/11449/878910.1016/j.micinf.2011.05.011WOS:000296216000008WOS000296216000008.pdf4066413997908572Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMicrobes and Infection2.9241,205info:eu-repo/semantics/openAccess2024-04-29T18:17:21Zoai:repositorio.unesp.br:11449/8789Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:34:31.860490Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Trypanocidal action of eupomatenoid-5 is related to mitochondrion dysfunction and oxidative damage in Trypanosoma cruzi |
title |
Trypanocidal action of eupomatenoid-5 is related to mitochondrion dysfunction and oxidative damage in Trypanosoma cruzi |
spellingShingle |
Trypanocidal action of eupomatenoid-5 is related to mitochondrion dysfunction and oxidative damage in Trypanosoma cruzi Pelizzaro-Rocha, Karin Juliane Trypanosoma cruzi Eupomatenoid-5 Antioxidant system |
title_short |
Trypanocidal action of eupomatenoid-5 is related to mitochondrion dysfunction and oxidative damage in Trypanosoma cruzi |
title_full |
Trypanocidal action of eupomatenoid-5 is related to mitochondrion dysfunction and oxidative damage in Trypanosoma cruzi |
title_fullStr |
Trypanocidal action of eupomatenoid-5 is related to mitochondrion dysfunction and oxidative damage in Trypanosoma cruzi |
title_full_unstemmed |
Trypanocidal action of eupomatenoid-5 is related to mitochondrion dysfunction and oxidative damage in Trypanosoma cruzi |
title_sort |
Trypanocidal action of eupomatenoid-5 is related to mitochondrion dysfunction and oxidative damage in Trypanosoma cruzi |
author |
Pelizzaro-Rocha, Karin Juliane |
author_facet |
Pelizzaro-Rocha, Karin Juliane Veiga-Santos, Phercyles Lazarin-Bidoia, Danielle Ueda-Nakamura, Tania Dias Filho, Benedito Prado Ximenes, Valdecir Farias [UNESP] Silva, Sueli Oliveira Nakamura, Celso Vataru |
author_role |
author |
author2 |
Veiga-Santos, Phercyles Lazarin-Bidoia, Danielle Ueda-Nakamura, Tania Dias Filho, Benedito Prado Ximenes, Valdecir Farias [UNESP] Silva, Sueli Oliveira Nakamura, Celso Vataru |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Londrina (UEL) Universidade Estadual de Maringá (UEM) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Pelizzaro-Rocha, Karin Juliane Veiga-Santos, Phercyles Lazarin-Bidoia, Danielle Ueda-Nakamura, Tania Dias Filho, Benedito Prado Ximenes, Valdecir Farias [UNESP] Silva, Sueli Oliveira Nakamura, Celso Vataru |
dc.subject.por.fl_str_mv |
Trypanosoma cruzi Eupomatenoid-5 Antioxidant system |
topic |
Trypanosoma cruzi Eupomatenoid-5 Antioxidant system |
description |
Because of its severe side effects and variable efficacy, the current treatment for Chagas disease is unsatisfactory. Natural compounds are good alternative chemotherapeutic agents for the treatment of this infection. Recently, our group reported the antiproliferative activity and morphological alterations in epimastigotes and intracellular amastigotes of Trypanosoma cruzi treated with eupomatenoid-5, a neolignan isolated from leaves of Piper regnellii var. pallescens. Here, we demonstrate that eupomatenoid-5 exhibited activity against trypomastigotes, the infective form of T cruzi (EC(50) 40.5 mu W), leading to ultrastructural alteration and lipoperoxidation in the cell membrane. Additionally, eupomatenoid-5 induced depolarization of the mitochondrial membrane, lipoperoxidation and increased G6PD activity in epimastigotes of T cruzi. These findings support the possibility that different mechanisms may be targeted, according to the form of the parasite, and that the plasma membrane and mitochondria are the structures that are most affected in trypomastigotes and epimastigotes, respectively. Thus, the trypanocidal action of eupomatenoid-5 may be associated with mitochondrial dysfunction and oxidative damage, which can trigger destructive effects on biological molecules of T cruzi, leading to parasite death. (C) 2011 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-11-01 2014-05-20T13:27:00Z 2014-05-20T13:27:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.micinf.2011.05.011 Microbes and Infection. Amsterdam: Elsevier B.V., v. 13, n. 12-13, p. 1018-1024, 2011. 1286-4579 http://hdl.handle.net/11449/8789 10.1016/j.micinf.2011.05.011 WOS:000296216000008 WOS000296216000008.pdf 4066413997908572 |
url |
http://dx.doi.org/10.1016/j.micinf.2011.05.011 http://hdl.handle.net/11449/8789 |
identifier_str_mv |
Microbes and Infection. Amsterdam: Elsevier B.V., v. 13, n. 12-13, p. 1018-1024, 2011. 1286-4579 10.1016/j.micinf.2011.05.011 WOS:000296216000008 WOS000296216000008.pdf 4066413997908572 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Microbes and Infection 2.924 1,205 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1018-1024 application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129439622496256 |