Targeting riboswitches with synthetic small RNAs for metabolic engineering
Autor(a) principal: | |
---|---|
Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.ymben.2021.09.003 http://hdl.handle.net/11449/222399 |
Resumo: | Our growing knowledge of the diversity of non-coding RNAs in natural systems and our deepening knowledge of RNA folding and function have fomented the rational design of RNA regulators. Based on that knowledge, we designed and implemented a small RNA tool to target bacterial riboswitches and activate gene expression (rtRNA). The synthetic rtRNA is suitable for regulation of gene expression both in cell-free and in cellular systems. It targets riboswitches to promote the antitermination folding regardless the cognate metabolite concentration. Therefore, it prevents transcription termination increasing gene expression up to 103-fold. We successfully used small RNA arrays for multiplex targeting of riboswitches. Finally, we used the synthetic rtRNAs to engineer an improved riboflavin producer strain. The easiness to design and construct, and the fact that the rtRNA works as a single genome copy, make it an attractive tool for engineering industrial metabolite-producing strains. |
id |
UNSP_55ea38d002290f18c16ab851bebea4ba |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/222399 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Targeting riboswitches with synthetic small RNAs for metabolic engineeringBacillus subtilisNon-coding RNARiboflavinRiboswitchSmall RNASynthetic biologyOur growing knowledge of the diversity of non-coding RNAs in natural systems and our deepening knowledge of RNA folding and function have fomented the rational design of RNA regulators. Based on that knowledge, we designed and implemented a small RNA tool to target bacterial riboswitches and activate gene expression (rtRNA). The synthetic rtRNA is suitable for regulation of gene expression both in cell-free and in cellular systems. It targets riboswitches to promote the antitermination folding regardless the cognate metabolite concentration. Therefore, it prevents transcription termination increasing gene expression up to 103-fold. We successfully used small RNA arrays for multiplex targeting of riboswitches. Finally, we used the synthetic rtRNAs to engineer an improved riboflavin producer strain. The easiness to design and construct, and the fact that the rtRNA works as a single genome copy, make it an attractive tool for engineering industrial metabolite-producing strains.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidade Estadual Paulista (UNESP) School of Pharmaceutical Sciences Department of Bioprocess Engineering and Biotechnology, Rodovia Araraquara-Jau Km1Mannheim University of Applied Sciences Institute for Technical Microbiology, Paul-Wittsack-Str. 10Universidade Estadual Paulista (UNESP) School of Pharmaceutical Sciences Department of Bioprocess Engineering and Biotechnology, Rodovia Araraquara-Jau Km1FAPESP: 2014/17564-7FAPESP: 2020/08699-7CNPq: 290110/2017-3Universidade Estadual Paulista (UNESP)Institute for Technical MicrobiologyLins, Milca Rachel da Costa Ribeiro [UNESP]Amorim, Laura Araujo da Silva [UNESP]Corrêa, Graciely Gomes [UNESP]Picão, Bruno Willian [UNESP]Mack, MatthiasCerri, Marcel Otávio [UNESP]Pedrolli, Danielle Biscaro [UNESP]2022-04-28T19:44:22Z2022-04-28T19:44:22Z2021-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article59-67http://dx.doi.org/10.1016/j.ymben.2021.09.003Metabolic Engineering, v. 68, p. 59-67.1096-71841096-7176http://hdl.handle.net/11449/22239910.1016/j.ymben.2021.09.0032-s2.0-85114819948Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMetabolic Engineeringinfo:eu-repo/semantics/openAccess2022-04-28T19:44:23Zoai:repositorio.unesp.br:11449/222399Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:59:44.228159Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Targeting riboswitches with synthetic small RNAs for metabolic engineering |
title |
Targeting riboswitches with synthetic small RNAs for metabolic engineering |
spellingShingle |
Targeting riboswitches with synthetic small RNAs for metabolic engineering Lins, Milca Rachel da Costa Ribeiro [UNESP] Bacillus subtilis Non-coding RNA Riboflavin Riboswitch Small RNA Synthetic biology |
title_short |
Targeting riboswitches with synthetic small RNAs for metabolic engineering |
title_full |
Targeting riboswitches with synthetic small RNAs for metabolic engineering |
title_fullStr |
Targeting riboswitches with synthetic small RNAs for metabolic engineering |
title_full_unstemmed |
Targeting riboswitches with synthetic small RNAs for metabolic engineering |
title_sort |
Targeting riboswitches with synthetic small RNAs for metabolic engineering |
author |
Lins, Milca Rachel da Costa Ribeiro [UNESP] |
author_facet |
Lins, Milca Rachel da Costa Ribeiro [UNESP] Amorim, Laura Araujo da Silva [UNESP] Corrêa, Graciely Gomes [UNESP] Picão, Bruno Willian [UNESP] Mack, Matthias Cerri, Marcel Otávio [UNESP] Pedrolli, Danielle Biscaro [UNESP] |
author_role |
author |
author2 |
Amorim, Laura Araujo da Silva [UNESP] Corrêa, Graciely Gomes [UNESP] Picão, Bruno Willian [UNESP] Mack, Matthias Cerri, Marcel Otávio [UNESP] Pedrolli, Danielle Biscaro [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Institute for Technical Microbiology |
dc.contributor.author.fl_str_mv |
Lins, Milca Rachel da Costa Ribeiro [UNESP] Amorim, Laura Araujo da Silva [UNESP] Corrêa, Graciely Gomes [UNESP] Picão, Bruno Willian [UNESP] Mack, Matthias Cerri, Marcel Otávio [UNESP] Pedrolli, Danielle Biscaro [UNESP] |
dc.subject.por.fl_str_mv |
Bacillus subtilis Non-coding RNA Riboflavin Riboswitch Small RNA Synthetic biology |
topic |
Bacillus subtilis Non-coding RNA Riboflavin Riboswitch Small RNA Synthetic biology |
description |
Our growing knowledge of the diversity of non-coding RNAs in natural systems and our deepening knowledge of RNA folding and function have fomented the rational design of RNA regulators. Based on that knowledge, we designed and implemented a small RNA tool to target bacterial riboswitches and activate gene expression (rtRNA). The synthetic rtRNA is suitable for regulation of gene expression both in cell-free and in cellular systems. It targets riboswitches to promote the antitermination folding regardless the cognate metabolite concentration. Therefore, it prevents transcription termination increasing gene expression up to 103-fold. We successfully used small RNA arrays for multiplex targeting of riboswitches. Finally, we used the synthetic rtRNAs to engineer an improved riboflavin producer strain. The easiness to design and construct, and the fact that the rtRNA works as a single genome copy, make it an attractive tool for engineering industrial metabolite-producing strains. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-11-01 2022-04-28T19:44:22Z 2022-04-28T19:44:22Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.ymben.2021.09.003 Metabolic Engineering, v. 68, p. 59-67. 1096-7184 1096-7176 http://hdl.handle.net/11449/222399 10.1016/j.ymben.2021.09.003 2-s2.0-85114819948 |
url |
http://dx.doi.org/10.1016/j.ymben.2021.09.003 http://hdl.handle.net/11449/222399 |
identifier_str_mv |
Metabolic Engineering, v. 68, p. 59-67. 1096-7184 1096-7176 10.1016/j.ymben.2021.09.003 2-s2.0-85114819948 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Metabolic Engineering |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
59-67 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128732701917184 |