Análise espermática de perdizes (Rhynchotus rufescens) criadas em cativeiro e suplementadas com selênio

Detalhes bibliográficos
Autor(a) principal: Góes, Paola Almeida de Araújo
Data de Publicação: 2011
Outros Autores: Cavalcante, Ana Karina da Silva, Tavian, Aline Frasseto [UNESP], Felipe, Leticia, Santos, Erika Coelho [UNESP], Nichi, Marcílio, de Queiroz, Sandra Aidar [UNESP], Barnabe, Renato Campanarut, Barnabe, Valquíria Hyppolito
Tipo de documento: Artigo
Idioma: eng
por
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://www.revistas.usp.br/bjvras/article/view/34402
http://hdl.handle.net/11449/73013
Resumo: Due to the commercial importance of the red-winged tinamou (Rhynchotus rufescens), for the past few years, the employment of reproductive biotechnologies has been attempted. Thirty animals were randomly assigned into two groups: control group (no selenium) and treatment group (supplemented with 0,2 a 0,8 mg selenium/ 1000 kg ration). Animals were allocated at the FCAV - UNESP/Jaboticabal (2007-2008). Semen collections were performed by digital manipulation and divided in pools of at least 150 μL. After the immediate evaluation of motility, vigour, concentration and morphology, an aliquot of 20 μL was diluted in 300 μL of physiologic solution in order to test acrosome and membrane integrities, which were performed by counting 200 cells for each test. Cells were evaluated as follows: 1) Intact acrosome: lilac acrosome; Non-intact acrosome: pink acrosome; 2) Live cells: non stained; Dead: stained. Data was statistically analysed using the SAS System for Windows. No differences were found between treatment and control groups for volume, motility, vigour, mean number of spermatozoa per animal, concentration, Intact acrosome, Intact membrane. The difference found on midpiece sperm defect (Se = 1,33 ± 0,53 and control = 3,78 ± 0,69, p = 0.0107) may be due to the damages caused by the selenium deficiency to the architecture of the midpiece, which compromises sperm mobility and fertilization capacity.
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spelling Análise espermática de perdizes (Rhynchotus rufescens) criadas em cativeiro e suplementadas com selênioSemen evaluation of captive male red-winged tinamous (Rhynchotus rufescens) supplemented with seleniumBird semen collectionRed-winged tinamouSeleniumAnimaliaAvesRhynchotus rufescensDue to the commercial importance of the red-winged tinamou (Rhynchotus rufescens), for the past few years, the employment of reproductive biotechnologies has been attempted. Thirty animals were randomly assigned into two groups: control group (no selenium) and treatment group (supplemented with 0,2 a 0,8 mg selenium/ 1000 kg ration). Animals were allocated at the FCAV - UNESP/Jaboticabal (2007-2008). Semen collections were performed by digital manipulation and divided in pools of at least 150 μL. After the immediate evaluation of motility, vigour, concentration and morphology, an aliquot of 20 μL was diluted in 300 μL of physiologic solution in order to test acrosome and membrane integrities, which were performed by counting 200 cells for each test. Cells were evaluated as follows: 1) Intact acrosome: lilac acrosome; Non-intact acrosome: pink acrosome; 2) Live cells: non stained; Dead: stained. Data was statistically analysed using the SAS System for Windows. No differences were found between treatment and control groups for volume, motility, vigour, mean number of spermatozoa per animal, concentration, Intact acrosome, Intact membrane. The difference found on midpiece sperm defect (Se = 1,33 ± 0,53 and control = 3,78 ± 0,69, p = 0.0107) may be due to the damages caused by the selenium deficiency to the architecture of the midpiece, which compromises sperm mobility and fertilization capacity.Departamento de Reprodução Animal Faculdade de Medicina Veterinária Zootecnia da Universidade de São Paulo, São Paulo- SPDepartamento de Reprodução Universidade Metropolitana de Salvador, Salvador-BahiaDepartamento de Zootecnia Faculdade de Ciências Agrárias e Veterinárias da Universidade Estadual Paulista, Jaboticabal - SPDepartamento de Zootecnia Faculdade de Ciências Agrárias e Veterinárias da Universidade Estadual Paulista, Jaboticabal - SPUniversidade de São Paulo (USP)Universidade Metropolitana de SalvadorUniversidade Estadual Paulista (Unesp)Góes, Paola Almeida de AraújoCavalcante, Ana Karina da SilvaTavian, Aline Frasseto [UNESP]Felipe, LeticiaSantos, Erika Coelho [UNESP]Nichi, Marcíliode Queiroz, Sandra Aidar [UNESP]Barnabe, Renato CampanarutBarnabe, Valquíria Hyppolito2014-05-27T11:26:18Z2014-05-27T11:26:18Z2011-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article370-377application/pdfhttp://www.revistas.usp.br/bjvras/article/view/34402Brazilian Journal of Veterinary Research and Animal Science, v. 48, n. 5, p. 370-377, 2011.1413-95961678-4456http://hdl.handle.net/11449/730132-s2.0-848569199672-s2.0-84856919967.pdf9096087557977610Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengporBrazilian Journal of Veterinary Research and Animal Science0,2250,225info:eu-repo/semantics/openAccess2024-06-07T18:41:30Zoai:repositorio.unesp.br:11449/73013Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:57:29.569631Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Análise espermática de perdizes (Rhynchotus rufescens) criadas em cativeiro e suplementadas com selênio
Semen evaluation of captive male red-winged tinamous (Rhynchotus rufescens) supplemented with selenium
title Análise espermática de perdizes (Rhynchotus rufescens) criadas em cativeiro e suplementadas com selênio
spellingShingle Análise espermática de perdizes (Rhynchotus rufescens) criadas em cativeiro e suplementadas com selênio
Góes, Paola Almeida de Araújo
Bird semen collection
Red-winged tinamou
Selenium
Animalia
Aves
Rhynchotus rufescens
title_short Análise espermática de perdizes (Rhynchotus rufescens) criadas em cativeiro e suplementadas com selênio
title_full Análise espermática de perdizes (Rhynchotus rufescens) criadas em cativeiro e suplementadas com selênio
title_fullStr Análise espermática de perdizes (Rhynchotus rufescens) criadas em cativeiro e suplementadas com selênio
title_full_unstemmed Análise espermática de perdizes (Rhynchotus rufescens) criadas em cativeiro e suplementadas com selênio
title_sort Análise espermática de perdizes (Rhynchotus rufescens) criadas em cativeiro e suplementadas com selênio
author Góes, Paola Almeida de Araújo
author_facet Góes, Paola Almeida de Araújo
Cavalcante, Ana Karina da Silva
Tavian, Aline Frasseto [UNESP]
Felipe, Leticia
Santos, Erika Coelho [UNESP]
Nichi, Marcílio
de Queiroz, Sandra Aidar [UNESP]
Barnabe, Renato Campanarut
Barnabe, Valquíria Hyppolito
author_role author
author2 Cavalcante, Ana Karina da Silva
Tavian, Aline Frasseto [UNESP]
Felipe, Leticia
Santos, Erika Coelho [UNESP]
Nichi, Marcílio
de Queiroz, Sandra Aidar [UNESP]
Barnabe, Renato Campanarut
Barnabe, Valquíria Hyppolito
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
Universidade Metropolitana de Salvador
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Góes, Paola Almeida de Araújo
Cavalcante, Ana Karina da Silva
Tavian, Aline Frasseto [UNESP]
Felipe, Leticia
Santos, Erika Coelho [UNESP]
Nichi, Marcílio
de Queiroz, Sandra Aidar [UNESP]
Barnabe, Renato Campanarut
Barnabe, Valquíria Hyppolito
dc.subject.por.fl_str_mv Bird semen collection
Red-winged tinamou
Selenium
Animalia
Aves
Rhynchotus rufescens
topic Bird semen collection
Red-winged tinamou
Selenium
Animalia
Aves
Rhynchotus rufescens
description Due to the commercial importance of the red-winged tinamou (Rhynchotus rufescens), for the past few years, the employment of reproductive biotechnologies has been attempted. Thirty animals were randomly assigned into two groups: control group (no selenium) and treatment group (supplemented with 0,2 a 0,8 mg selenium/ 1000 kg ration). Animals were allocated at the FCAV - UNESP/Jaboticabal (2007-2008). Semen collections were performed by digital manipulation and divided in pools of at least 150 μL. After the immediate evaluation of motility, vigour, concentration and morphology, an aliquot of 20 μL was diluted in 300 μL of physiologic solution in order to test acrosome and membrane integrities, which were performed by counting 200 cells for each test. Cells were evaluated as follows: 1) Intact acrosome: lilac acrosome; Non-intact acrosome: pink acrosome; 2) Live cells: non stained; Dead: stained. Data was statistically analysed using the SAS System for Windows. No differences were found between treatment and control groups for volume, motility, vigour, mean number of spermatozoa per animal, concentration, Intact acrosome, Intact membrane. The difference found on midpiece sperm defect (Se = 1,33 ± 0,53 and control = 3,78 ± 0,69, p = 0.0107) may be due to the damages caused by the selenium deficiency to the architecture of the midpiece, which compromises sperm mobility and fertilization capacity.
publishDate 2011
dc.date.none.fl_str_mv 2011-12-01
2014-05-27T11:26:18Z
2014-05-27T11:26:18Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.revistas.usp.br/bjvras/article/view/34402
Brazilian Journal of Veterinary Research and Animal Science, v. 48, n. 5, p. 370-377, 2011.
1413-9596
1678-4456
http://hdl.handle.net/11449/73013
2-s2.0-84856919967
2-s2.0-84856919967.pdf
9096087557977610
url http://www.revistas.usp.br/bjvras/article/view/34402
http://hdl.handle.net/11449/73013
identifier_str_mv Brazilian Journal of Veterinary Research and Animal Science, v. 48, n. 5, p. 370-377, 2011.
1413-9596
1678-4456
2-s2.0-84856919967
2-s2.0-84856919967.pdf
9096087557977610
dc.language.iso.fl_str_mv eng
por
language eng
por
dc.relation.none.fl_str_mv Brazilian Journal of Veterinary Research and Animal Science
0,225
0,225
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 370-377
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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