Análise espermática de perdizes (Rhynchotus rufescens) criadas em cativeiro e suplementadas com selênio
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://www.revistas.usp.br/bjvras/article/view/34402 http://hdl.handle.net/11449/73013 |
Resumo: | Due to the commercial importance of the red-winged tinamou (Rhynchotus rufescens), for the past few years, the employment of reproductive biotechnologies has been attempted. Thirty animals were randomly assigned into two groups: control group (no selenium) and treatment group (supplemented with 0,2 a 0,8 mg selenium/ 1000 kg ration). Animals were allocated at the FCAV - UNESP/Jaboticabal (2007-2008). Semen collections were performed by digital manipulation and divided in pools of at least 150 μL. After the immediate evaluation of motility, vigour, concentration and morphology, an aliquot of 20 μL was diluted in 300 μL of physiologic solution in order to test acrosome and membrane integrities, which were performed by counting 200 cells for each test. Cells were evaluated as follows: 1) Intact acrosome: lilac acrosome; Non-intact acrosome: pink acrosome; 2) Live cells: non stained; Dead: stained. Data was statistically analysed using the SAS System for Windows. No differences were found between treatment and control groups for volume, motility, vigour, mean number of spermatozoa per animal, concentration, Intact acrosome, Intact membrane. The difference found on midpiece sperm defect (Se = 1,33 ± 0,53 and control = 3,78 ± 0,69, p = 0.0107) may be due to the damages caused by the selenium deficiency to the architecture of the midpiece, which compromises sperm mobility and fertilization capacity. |
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Análise espermática de perdizes (Rhynchotus rufescens) criadas em cativeiro e suplementadas com selênioSemen evaluation of captive male red-winged tinamous (Rhynchotus rufescens) supplemented with seleniumBird semen collectionRed-winged tinamouSeleniumAnimaliaAvesRhynchotus rufescensDue to the commercial importance of the red-winged tinamou (Rhynchotus rufescens), for the past few years, the employment of reproductive biotechnologies has been attempted. Thirty animals were randomly assigned into two groups: control group (no selenium) and treatment group (supplemented with 0,2 a 0,8 mg selenium/ 1000 kg ration). Animals were allocated at the FCAV - UNESP/Jaboticabal (2007-2008). Semen collections were performed by digital manipulation and divided in pools of at least 150 μL. After the immediate evaluation of motility, vigour, concentration and morphology, an aliquot of 20 μL was diluted in 300 μL of physiologic solution in order to test acrosome and membrane integrities, which were performed by counting 200 cells for each test. Cells were evaluated as follows: 1) Intact acrosome: lilac acrosome; Non-intact acrosome: pink acrosome; 2) Live cells: non stained; Dead: stained. Data was statistically analysed using the SAS System for Windows. No differences were found between treatment and control groups for volume, motility, vigour, mean number of spermatozoa per animal, concentration, Intact acrosome, Intact membrane. The difference found on midpiece sperm defect (Se = 1,33 ± 0,53 and control = 3,78 ± 0,69, p = 0.0107) may be due to the damages caused by the selenium deficiency to the architecture of the midpiece, which compromises sperm mobility and fertilization capacity.Departamento de Reprodução Animal Faculdade de Medicina Veterinária Zootecnia da Universidade de São Paulo, São Paulo- SPDepartamento de Reprodução Universidade Metropolitana de Salvador, Salvador-BahiaDepartamento de Zootecnia Faculdade de Ciências Agrárias e Veterinárias da Universidade Estadual Paulista, Jaboticabal - SPDepartamento de Zootecnia Faculdade de Ciências Agrárias e Veterinárias da Universidade Estadual Paulista, Jaboticabal - SPUniversidade de São Paulo (USP)Universidade Metropolitana de SalvadorUniversidade Estadual Paulista (Unesp)Góes, Paola Almeida de AraújoCavalcante, Ana Karina da SilvaTavian, Aline Frasseto [UNESP]Felipe, LeticiaSantos, Erika Coelho [UNESP]Nichi, Marcíliode Queiroz, Sandra Aidar [UNESP]Barnabe, Renato CampanarutBarnabe, Valquíria Hyppolito2014-05-27T11:26:18Z2014-05-27T11:26:18Z2011-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article370-377application/pdfhttp://www.revistas.usp.br/bjvras/article/view/34402Brazilian Journal of Veterinary Research and Animal Science, v. 48, n. 5, p. 370-377, 2011.1413-95961678-4456http://hdl.handle.net/11449/730132-s2.0-848569199672-s2.0-84856919967.pdf9096087557977610Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengporBrazilian Journal of Veterinary Research and Animal Science0,2250,225info:eu-repo/semantics/openAccess2024-06-07T18:41:30Zoai:repositorio.unesp.br:11449/73013Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:57:29.569631Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Análise espermática de perdizes (Rhynchotus rufescens) criadas em cativeiro e suplementadas com selênio Semen evaluation of captive male red-winged tinamous (Rhynchotus rufescens) supplemented with selenium |
title |
Análise espermática de perdizes (Rhynchotus rufescens) criadas em cativeiro e suplementadas com selênio |
spellingShingle |
Análise espermática de perdizes (Rhynchotus rufescens) criadas em cativeiro e suplementadas com selênio Góes, Paola Almeida de Araújo Bird semen collection Red-winged tinamou Selenium Animalia Aves Rhynchotus rufescens |
title_short |
Análise espermática de perdizes (Rhynchotus rufescens) criadas em cativeiro e suplementadas com selênio |
title_full |
Análise espermática de perdizes (Rhynchotus rufescens) criadas em cativeiro e suplementadas com selênio |
title_fullStr |
Análise espermática de perdizes (Rhynchotus rufescens) criadas em cativeiro e suplementadas com selênio |
title_full_unstemmed |
Análise espermática de perdizes (Rhynchotus rufescens) criadas em cativeiro e suplementadas com selênio |
title_sort |
Análise espermática de perdizes (Rhynchotus rufescens) criadas em cativeiro e suplementadas com selênio |
author |
Góes, Paola Almeida de Araújo |
author_facet |
Góes, Paola Almeida de Araújo Cavalcante, Ana Karina da Silva Tavian, Aline Frasseto [UNESP] Felipe, Leticia Santos, Erika Coelho [UNESP] Nichi, Marcílio de Queiroz, Sandra Aidar [UNESP] Barnabe, Renato Campanarut Barnabe, Valquíria Hyppolito |
author_role |
author |
author2 |
Cavalcante, Ana Karina da Silva Tavian, Aline Frasseto [UNESP] Felipe, Leticia Santos, Erika Coelho [UNESP] Nichi, Marcílio de Queiroz, Sandra Aidar [UNESP] Barnabe, Renato Campanarut Barnabe, Valquíria Hyppolito |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Metropolitana de Salvador Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Góes, Paola Almeida de Araújo Cavalcante, Ana Karina da Silva Tavian, Aline Frasseto [UNESP] Felipe, Leticia Santos, Erika Coelho [UNESP] Nichi, Marcílio de Queiroz, Sandra Aidar [UNESP] Barnabe, Renato Campanarut Barnabe, Valquíria Hyppolito |
dc.subject.por.fl_str_mv |
Bird semen collection Red-winged tinamou Selenium Animalia Aves Rhynchotus rufescens |
topic |
Bird semen collection Red-winged tinamou Selenium Animalia Aves Rhynchotus rufescens |
description |
Due to the commercial importance of the red-winged tinamou (Rhynchotus rufescens), for the past few years, the employment of reproductive biotechnologies has been attempted. Thirty animals were randomly assigned into two groups: control group (no selenium) and treatment group (supplemented with 0,2 a 0,8 mg selenium/ 1000 kg ration). Animals were allocated at the FCAV - UNESP/Jaboticabal (2007-2008). Semen collections were performed by digital manipulation and divided in pools of at least 150 μL. After the immediate evaluation of motility, vigour, concentration and morphology, an aliquot of 20 μL was diluted in 300 μL of physiologic solution in order to test acrosome and membrane integrities, which were performed by counting 200 cells for each test. Cells were evaluated as follows: 1) Intact acrosome: lilac acrosome; Non-intact acrosome: pink acrosome; 2) Live cells: non stained; Dead: stained. Data was statistically analysed using the SAS System for Windows. No differences were found between treatment and control groups for volume, motility, vigour, mean number of spermatozoa per animal, concentration, Intact acrosome, Intact membrane. The difference found on midpiece sperm defect (Se = 1,33 ± 0,53 and control = 3,78 ± 0,69, p = 0.0107) may be due to the damages caused by the selenium deficiency to the architecture of the midpiece, which compromises sperm mobility and fertilization capacity. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-12-01 2014-05-27T11:26:18Z 2014-05-27T11:26:18Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.revistas.usp.br/bjvras/article/view/34402 Brazilian Journal of Veterinary Research and Animal Science, v. 48, n. 5, p. 370-377, 2011. 1413-9596 1678-4456 http://hdl.handle.net/11449/73013 2-s2.0-84856919967 2-s2.0-84856919967.pdf 9096087557977610 |
url |
http://www.revistas.usp.br/bjvras/article/view/34402 http://hdl.handle.net/11449/73013 |
identifier_str_mv |
Brazilian Journal of Veterinary Research and Animal Science, v. 48, n. 5, p. 370-377, 2011. 1413-9596 1678-4456 2-s2.0-84856919967 2-s2.0-84856919967.pdf 9096087557977610 |
dc.language.iso.fl_str_mv |
eng por |
language |
eng por |
dc.relation.none.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science 0,225 0,225 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
370-377 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128878944714752 |