Mercury Exposure: Protein Biomarkers of Mercury Exposure in Jaraqui Fish from the Amazon Region
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s12011-017-1129-5 http://hdl.handle.net/11449/170053 |
Resumo: | This study presents data on the extraction and characterization of proteins associated with mercury in the muscle and liver tissues of jaraqui (Semaprochilodus spp.) from the Madeira River in the Brazilian Amazon. Protein fractionation was carried out by two-dimensional electrophoresis (2D-PAGE). Mercury determination in tissues, pellets, and protein spots was performed by graphite furnace atomic absorption spectrometry (GFAAS). Proteins in the spots that showed mercury were characterized by electrospray ionization tandem mass spectrometry (ESI-MS/MS). The highest mercury concentrations were found in liver tissues and pellets (426 ± 6 and 277 ± 4 μg kg−1), followed by muscle tissues and pellets (132 ± 4 and 86 ± 1 μg kg−1, respectively). Mercury quantification in the protein spots allowed us to propose stoichiometric ratios in the range of 1–4 mercury atoms per molecule of protein in the protein spots. The proteins characterized in the analysis by ESI-MS/MS were keratin, type II cytoskeletal 8, parvalbumin beta, parvalbumin-2, ubiquitin-40S ribosomal S27a, 39S ribosomal protein L36 mitochondrial, hemoglobin subunit beta, and hemoglobin subunit beta-A/B. The results suggest that proteins such as ubiquitin-40S ribosomal protein S27a, which have specific domains, possibly zinc finger, can be used as biomarkers of mercury, whereas mercury and zinc present characteristics of soft acids. |
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Mercury Exposure: Protein Biomarkers of Mercury Exposure in Jaraqui Fish from the Amazon Region2D-PAGEBiomarkers of mercury exposureESI-MS/MSMercury in fishMetallomics studyThis study presents data on the extraction and characterization of proteins associated with mercury in the muscle and liver tissues of jaraqui (Semaprochilodus spp.) from the Madeira River in the Brazilian Amazon. Protein fractionation was carried out by two-dimensional electrophoresis (2D-PAGE). Mercury determination in tissues, pellets, and protein spots was performed by graphite furnace atomic absorption spectrometry (GFAAS). Proteins in the spots that showed mercury were characterized by electrospray ionization tandem mass spectrometry (ESI-MS/MS). The highest mercury concentrations were found in liver tissues and pellets (426 ± 6 and 277 ± 4 μg kg−1), followed by muscle tissues and pellets (132 ± 4 and 86 ± 1 μg kg−1, respectively). Mercury quantification in the protein spots allowed us to propose stoichiometric ratios in the range of 1–4 mercury atoms per molecule of protein in the protein spots. The proteins characterized in the analysis by ESI-MS/MS were keratin, type II cytoskeletal 8, parvalbumin beta, parvalbumin-2, ubiquitin-40S ribosomal S27a, 39S ribosomal protein L36 mitochondrial, hemoglobin subunit beta, and hemoglobin subunit beta-A/B. The results suggest that proteins such as ubiquitin-40S ribosomal protein S27a, which have specific domains, possibly zinc finger, can be used as biomarkers of mercury, whereas mercury and zinc present characteristics of soft acids.Institute of Bioscience of Botucatu São Paulo State University (UNESP), Rubião JúniorPontifical Catholic University of Goiás (PUC)Bauru Dental School University of São Paulo-USPInstitute of Bioscience of Botucatu São Paulo State University (UNESP), Rubião JúniorUniversidade Estadual Paulista (Unesp)Pontifical Catholic University of Goiás (PUC)Universidade de São Paulo (USP)Vieira, José Cavalcante Souza [UNESP]Braga, Camila Pereira [UNESP]de Oliveira, Grasieli [UNESP]Padilha, Cilenedo Carmo Federici [UNESP]de Moraes, Paula Martin [UNESP]Zara, Luiz FabricioLeite, Alinede LimaBuzalaf, Marília Afonso RabeloPadilha, Pedrode Magalhães [UNESP]2018-12-11T16:48:54Z2018-12-11T16:48:54Z2018-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article164-171application/pdfhttp://dx.doi.org/10.1007/s12011-017-1129-5Biological Trace Element Research, v. 183, n. 1, p. 164-171, 2018.1559-07200163-4984http://hdl.handle.net/11449/17005310.1007/s12011-017-1129-52-s2.0-850279915302-s2.0-85027991530.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiological Trace Element Research0,719info:eu-repo/semantics/openAccess2023-12-17T06:24:12Zoai:repositorio.unesp.br:11449/170053Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:36:38.251205Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Mercury Exposure: Protein Biomarkers of Mercury Exposure in Jaraqui Fish from the Amazon Region |
title |
Mercury Exposure: Protein Biomarkers of Mercury Exposure in Jaraqui Fish from the Amazon Region |
spellingShingle |
Mercury Exposure: Protein Biomarkers of Mercury Exposure in Jaraqui Fish from the Amazon Region Vieira, José Cavalcante Souza [UNESP] 2D-PAGE Biomarkers of mercury exposure ESI-MS/MS Mercury in fish Metallomics study |
title_short |
Mercury Exposure: Protein Biomarkers of Mercury Exposure in Jaraqui Fish from the Amazon Region |
title_full |
Mercury Exposure: Protein Biomarkers of Mercury Exposure in Jaraqui Fish from the Amazon Region |
title_fullStr |
Mercury Exposure: Protein Biomarkers of Mercury Exposure in Jaraqui Fish from the Amazon Region |
title_full_unstemmed |
Mercury Exposure: Protein Biomarkers of Mercury Exposure in Jaraqui Fish from the Amazon Region |
title_sort |
Mercury Exposure: Protein Biomarkers of Mercury Exposure in Jaraqui Fish from the Amazon Region |
author |
Vieira, José Cavalcante Souza [UNESP] |
author_facet |
Vieira, José Cavalcante Souza [UNESP] Braga, Camila Pereira [UNESP] de Oliveira, Grasieli [UNESP] Padilha, Cilenedo Carmo Federici [UNESP] de Moraes, Paula Martin [UNESP] Zara, Luiz Fabricio Leite, Alinede Lima Buzalaf, Marília Afonso Rabelo Padilha, Pedrode Magalhães [UNESP] |
author_role |
author |
author2 |
Braga, Camila Pereira [UNESP] de Oliveira, Grasieli [UNESP] Padilha, Cilenedo Carmo Federici [UNESP] de Moraes, Paula Martin [UNESP] Zara, Luiz Fabricio Leite, Alinede Lima Buzalaf, Marília Afonso Rabelo Padilha, Pedrode Magalhães [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Pontifical Catholic University of Goiás (PUC) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Vieira, José Cavalcante Souza [UNESP] Braga, Camila Pereira [UNESP] de Oliveira, Grasieli [UNESP] Padilha, Cilenedo Carmo Federici [UNESP] de Moraes, Paula Martin [UNESP] Zara, Luiz Fabricio Leite, Alinede Lima Buzalaf, Marília Afonso Rabelo Padilha, Pedrode Magalhães [UNESP] |
dc.subject.por.fl_str_mv |
2D-PAGE Biomarkers of mercury exposure ESI-MS/MS Mercury in fish Metallomics study |
topic |
2D-PAGE Biomarkers of mercury exposure ESI-MS/MS Mercury in fish Metallomics study |
description |
This study presents data on the extraction and characterization of proteins associated with mercury in the muscle and liver tissues of jaraqui (Semaprochilodus spp.) from the Madeira River in the Brazilian Amazon. Protein fractionation was carried out by two-dimensional electrophoresis (2D-PAGE). Mercury determination in tissues, pellets, and protein spots was performed by graphite furnace atomic absorption spectrometry (GFAAS). Proteins in the spots that showed mercury were characterized by electrospray ionization tandem mass spectrometry (ESI-MS/MS). The highest mercury concentrations were found in liver tissues and pellets (426 ± 6 and 277 ± 4 μg kg−1), followed by muscle tissues and pellets (132 ± 4 and 86 ± 1 μg kg−1, respectively). Mercury quantification in the protein spots allowed us to propose stoichiometric ratios in the range of 1–4 mercury atoms per molecule of protein in the protein spots. The proteins characterized in the analysis by ESI-MS/MS were keratin, type II cytoskeletal 8, parvalbumin beta, parvalbumin-2, ubiquitin-40S ribosomal S27a, 39S ribosomal protein L36 mitochondrial, hemoglobin subunit beta, and hemoglobin subunit beta-A/B. The results suggest that proteins such as ubiquitin-40S ribosomal protein S27a, which have specific domains, possibly zinc finger, can be used as biomarkers of mercury, whereas mercury and zinc present characteristics of soft acids. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T16:48:54Z 2018-12-11T16:48:54Z 2018-05-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s12011-017-1129-5 Biological Trace Element Research, v. 183, n. 1, p. 164-171, 2018. 1559-0720 0163-4984 http://hdl.handle.net/11449/170053 10.1007/s12011-017-1129-5 2-s2.0-85027991530 2-s2.0-85027991530.pdf |
url |
http://dx.doi.org/10.1007/s12011-017-1129-5 http://hdl.handle.net/11449/170053 |
identifier_str_mv |
Biological Trace Element Research, v. 183, n. 1, p. 164-171, 2018. 1559-0720 0163-4984 10.1007/s12011-017-1129-5 2-s2.0-85027991530 2-s2.0-85027991530.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Biological Trace Element Research 0,719 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
164-171 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129226045390848 |