Mercury Exposure: Protein Biomarkers of Mercury Exposure in Jaraqui Fish from the Amazon Region

Detalhes bibliográficos
Autor(a) principal: Vieira, José Cavalcante Souza [UNESP]
Data de Publicação: 2018
Outros Autores: Braga, Camila Pereira [UNESP], de Oliveira, Grasieli [UNESP], Padilha, Cilenedo Carmo Federici [UNESP], de Moraes, Paula Martin [UNESP], Zara, Luiz Fabricio, Leite, Alinede Lima, Buzalaf, Marília Afonso Rabelo, Padilha, Pedrode Magalhães [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/s12011-017-1129-5
http://hdl.handle.net/11449/170053
Resumo: This study presents data on the extraction and characterization of proteins associated with mercury in the muscle and liver tissues of jaraqui (Semaprochilodus spp.) from the Madeira River in the Brazilian Amazon. Protein fractionation was carried out by two-dimensional electrophoresis (2D-PAGE). Mercury determination in tissues, pellets, and protein spots was performed by graphite furnace atomic absorption spectrometry (GFAAS). Proteins in the spots that showed mercury were characterized by electrospray ionization tandem mass spectrometry (ESI-MS/MS). The highest mercury concentrations were found in liver tissues and pellets (426 ± 6 and 277 ± 4 μg kg−1), followed by muscle tissues and pellets (132 ± 4 and 86 ± 1 μg kg−1, respectively). Mercury quantification in the protein spots allowed us to propose stoichiometric ratios in the range of 1–4 mercury atoms per molecule of protein in the protein spots. The proteins characterized in the analysis by ESI-MS/MS were keratin, type II cytoskeletal 8, parvalbumin beta, parvalbumin-2, ubiquitin-40S ribosomal S27a, 39S ribosomal protein L36 mitochondrial, hemoglobin subunit beta, and hemoglobin subunit beta-A/B. The results suggest that proteins such as ubiquitin-40S ribosomal protein S27a, which have specific domains, possibly zinc finger, can be used as biomarkers of mercury, whereas mercury and zinc present characteristics of soft acids.
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spelling Mercury Exposure: Protein Biomarkers of Mercury Exposure in Jaraqui Fish from the Amazon Region2D-PAGEBiomarkers of mercury exposureESI-MS/MSMercury in fishMetallomics studyThis study presents data on the extraction and characterization of proteins associated with mercury in the muscle and liver tissues of jaraqui (Semaprochilodus spp.) from the Madeira River in the Brazilian Amazon. Protein fractionation was carried out by two-dimensional electrophoresis (2D-PAGE). Mercury determination in tissues, pellets, and protein spots was performed by graphite furnace atomic absorption spectrometry (GFAAS). Proteins in the spots that showed mercury were characterized by electrospray ionization tandem mass spectrometry (ESI-MS/MS). The highest mercury concentrations were found in liver tissues and pellets (426 ± 6 and 277 ± 4 μg kg−1), followed by muscle tissues and pellets (132 ± 4 and 86 ± 1 μg kg−1, respectively). Mercury quantification in the protein spots allowed us to propose stoichiometric ratios in the range of 1–4 mercury atoms per molecule of protein in the protein spots. The proteins characterized in the analysis by ESI-MS/MS were keratin, type II cytoskeletal 8, parvalbumin beta, parvalbumin-2, ubiquitin-40S ribosomal S27a, 39S ribosomal protein L36 mitochondrial, hemoglobin subunit beta, and hemoglobin subunit beta-A/B. The results suggest that proteins such as ubiquitin-40S ribosomal protein S27a, which have specific domains, possibly zinc finger, can be used as biomarkers of mercury, whereas mercury and zinc present characteristics of soft acids.Institute of Bioscience of Botucatu São Paulo State University (UNESP), Rubião JúniorPontifical Catholic University of Goiás (PUC)Bauru Dental School University of São Paulo-USPInstitute of Bioscience of Botucatu São Paulo State University (UNESP), Rubião JúniorUniversidade Estadual Paulista (Unesp)Pontifical Catholic University of Goiás (PUC)Universidade de São Paulo (USP)Vieira, José Cavalcante Souza [UNESP]Braga, Camila Pereira [UNESP]de Oliveira, Grasieli [UNESP]Padilha, Cilenedo Carmo Federici [UNESP]de Moraes, Paula Martin [UNESP]Zara, Luiz FabricioLeite, Alinede LimaBuzalaf, Marília Afonso RabeloPadilha, Pedrode Magalhães [UNESP]2018-12-11T16:48:54Z2018-12-11T16:48:54Z2018-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article164-171application/pdfhttp://dx.doi.org/10.1007/s12011-017-1129-5Biological Trace Element Research, v. 183, n. 1, p. 164-171, 2018.1559-07200163-4984http://hdl.handle.net/11449/17005310.1007/s12011-017-1129-52-s2.0-850279915302-s2.0-85027991530.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiological Trace Element Research0,719info:eu-repo/semantics/openAccess2023-12-17T06:24:12Zoai:repositorio.unesp.br:11449/170053Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:36:38.251205Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Mercury Exposure: Protein Biomarkers of Mercury Exposure in Jaraqui Fish from the Amazon Region
title Mercury Exposure: Protein Biomarkers of Mercury Exposure in Jaraqui Fish from the Amazon Region
spellingShingle Mercury Exposure: Protein Biomarkers of Mercury Exposure in Jaraqui Fish from the Amazon Region
Vieira, José Cavalcante Souza [UNESP]
2D-PAGE
Biomarkers of mercury exposure
ESI-MS/MS
Mercury in fish
Metallomics study
title_short Mercury Exposure: Protein Biomarkers of Mercury Exposure in Jaraqui Fish from the Amazon Region
title_full Mercury Exposure: Protein Biomarkers of Mercury Exposure in Jaraqui Fish from the Amazon Region
title_fullStr Mercury Exposure: Protein Biomarkers of Mercury Exposure in Jaraqui Fish from the Amazon Region
title_full_unstemmed Mercury Exposure: Protein Biomarkers of Mercury Exposure in Jaraqui Fish from the Amazon Region
title_sort Mercury Exposure: Protein Biomarkers of Mercury Exposure in Jaraqui Fish from the Amazon Region
author Vieira, José Cavalcante Souza [UNESP]
author_facet Vieira, José Cavalcante Souza [UNESP]
Braga, Camila Pereira [UNESP]
de Oliveira, Grasieli [UNESP]
Padilha, Cilenedo Carmo Federici [UNESP]
de Moraes, Paula Martin [UNESP]
Zara, Luiz Fabricio
Leite, Alinede Lima
Buzalaf, Marília Afonso Rabelo
Padilha, Pedrode Magalhães [UNESP]
author_role author
author2 Braga, Camila Pereira [UNESP]
de Oliveira, Grasieli [UNESP]
Padilha, Cilenedo Carmo Federici [UNESP]
de Moraes, Paula Martin [UNESP]
Zara, Luiz Fabricio
Leite, Alinede Lima
Buzalaf, Marília Afonso Rabelo
Padilha, Pedrode Magalhães [UNESP]
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Pontifical Catholic University of Goiás (PUC)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Vieira, José Cavalcante Souza [UNESP]
Braga, Camila Pereira [UNESP]
de Oliveira, Grasieli [UNESP]
Padilha, Cilenedo Carmo Federici [UNESP]
de Moraes, Paula Martin [UNESP]
Zara, Luiz Fabricio
Leite, Alinede Lima
Buzalaf, Marília Afonso Rabelo
Padilha, Pedrode Magalhães [UNESP]
dc.subject.por.fl_str_mv 2D-PAGE
Biomarkers of mercury exposure
ESI-MS/MS
Mercury in fish
Metallomics study
topic 2D-PAGE
Biomarkers of mercury exposure
ESI-MS/MS
Mercury in fish
Metallomics study
description This study presents data on the extraction and characterization of proteins associated with mercury in the muscle and liver tissues of jaraqui (Semaprochilodus spp.) from the Madeira River in the Brazilian Amazon. Protein fractionation was carried out by two-dimensional electrophoresis (2D-PAGE). Mercury determination in tissues, pellets, and protein spots was performed by graphite furnace atomic absorption spectrometry (GFAAS). Proteins in the spots that showed mercury were characterized by electrospray ionization tandem mass spectrometry (ESI-MS/MS). The highest mercury concentrations were found in liver tissues and pellets (426 ± 6 and 277 ± 4 μg kg−1), followed by muscle tissues and pellets (132 ± 4 and 86 ± 1 μg kg−1, respectively). Mercury quantification in the protein spots allowed us to propose stoichiometric ratios in the range of 1–4 mercury atoms per molecule of protein in the protein spots. The proteins characterized in the analysis by ESI-MS/MS were keratin, type II cytoskeletal 8, parvalbumin beta, parvalbumin-2, ubiquitin-40S ribosomal S27a, 39S ribosomal protein L36 mitochondrial, hemoglobin subunit beta, and hemoglobin subunit beta-A/B. The results suggest that proteins such as ubiquitin-40S ribosomal protein S27a, which have specific domains, possibly zinc finger, can be used as biomarkers of mercury, whereas mercury and zinc present characteristics of soft acids.
publishDate 2018
dc.date.none.fl_str_mv 2018-12-11T16:48:54Z
2018-12-11T16:48:54Z
2018-05-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s12011-017-1129-5
Biological Trace Element Research, v. 183, n. 1, p. 164-171, 2018.
1559-0720
0163-4984
http://hdl.handle.net/11449/170053
10.1007/s12011-017-1129-5
2-s2.0-85027991530
2-s2.0-85027991530.pdf
url http://dx.doi.org/10.1007/s12011-017-1129-5
http://hdl.handle.net/11449/170053
identifier_str_mv Biological Trace Element Research, v. 183, n. 1, p. 164-171, 2018.
1559-0720
0163-4984
10.1007/s12011-017-1129-5
2-s2.0-85027991530
2-s2.0-85027991530.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Biological Trace Element Research
0,719
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 164-171
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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