Synthesis, characterization and applications of maghemite beads functionalized with rabbit antibodies
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1088/1361-6528/aacc21 http://hdl.handle.net/11449/171219 |
Resumo: | Magnetic nanoparticles (NPs) have attracted great attention owing to their applications in the biomedical field. In the present work, maghemite (γFe2O3) NPs of 6.5 nm were prepared using a sonochemical method and used to prepare magnetic beads through silanization with 3-aminopropyltrimethoxysilane (APTS). Subsequently, amino groups in the resulting APTS-γFe2O3 beads were converted to carboxylic acid (CARB-γFe2O3) through the succinic anhydride reaction, as confirmed by transmission electron microscopy (TEM), Fourier transform infrared spectroscopy and dynamic light scattering (DLS) measurements. The size of these beads was measured as 12 nm and their hydrodynamic diameter as 490 nm, using TEM analysis and DLS, respectively. The CARB-γFe2O3 beads were further functionalized by immobilizing rabbit antibodies on their surfaces; the immobilization was confirmed by flow cytometry and ionic strength. The samples were further characterized by Mössbauer spectroscopy and DC magnetization measurements. Studies on magnetic relaxivities showed that magnetic beads present great potential for application in MR imaging. |
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Synthesis, characterization and applications of maghemite beads functionalized with rabbit antibodiesflow cytometrymagnetic nanobeadsmossbauer spectroscopyMRIrabbit antibodyXRDMagnetic nanoparticles (NPs) have attracted great attention owing to their applications in the biomedical field. In the present work, maghemite (γFe2O3) NPs of 6.5 nm were prepared using a sonochemical method and used to prepare magnetic beads through silanization with 3-aminopropyltrimethoxysilane (APTS). Subsequently, amino groups in the resulting APTS-γFe2O3 beads were converted to carboxylic acid (CARB-γFe2O3) through the succinic anhydride reaction, as confirmed by transmission electron microscopy (TEM), Fourier transform infrared spectroscopy and dynamic light scattering (DLS) measurements. The size of these beads was measured as 12 nm and their hydrodynamic diameter as 490 nm, using TEM analysis and DLS, respectively. The CARB-γFe2O3 beads were further functionalized by immobilizing rabbit antibodies on their surfaces; the immobilization was confirmed by flow cytometry and ionic strength. The samples were further characterized by Mössbauer spectroscopy and DC magnetization measurements. Studies on magnetic relaxivities showed that magnetic beads present great potential for application in MR imaging.Federal University of Acre Laboratory of NanobiotechnologyFederal University of Rio Grande Do Norte Department of Theoretical and Experimental PhysicsDivision of Functional Materials and Nano-Devices Ningbo Institute of Materials Technology and Engineering Chinese Academy of SciencesInstituto Leonidas e Maria Deane Fundacao Oswaldo CruzSao Paulo State University - UNESP Laboratory of Magnetic Materials and Colloids Institute of Chemistry, Caixa postal 355Sao Paulo State University - UNESP Laboratory of Magnetic Materials and Colloids Institute of Chemistry, Caixa postal 355Laboratory of NanobiotechnologyFederal University of Rio Grande Do NorteChinese Academy of SciencesFundacao Oswaldo CruzUniversidade Estadual Paulista (Unesp)Rodriguez, A. F.R.Rocha, C. O. [UNESP]Piazza, R. D. [UNESP]Dos Santos, C. C. [UNESP]Morales, M. A.Faria, F. S.E.D.V.Iqbal, M ZubairBarbosa, L.Chaves, Y. O.Mariuba, L. A.Jafelicci, M. [UNESP]Marques, R. F.C. [UNESP]2018-12-11T16:54:27Z2018-12-11T16:54:27Z2018-06-28info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1088/1361-6528/aacc21Nanotechnology, v. 29, n. 36, 2018.1361-65280957-4484http://hdl.handle.net/11449/17121910.1088/1361-6528/aacc212-s2.0-850499787552-s2.0-85049978755.pdf21159426216941740000-0003-0195-3885Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengNanotechnology1,0791,079info:eu-repo/semantics/openAccess2023-10-12T06:07:19Zoai:repositorio.unesp.br:11449/171219Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:41:26.950725Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Synthesis, characterization and applications of maghemite beads functionalized with rabbit antibodies |
title |
Synthesis, characterization and applications of maghemite beads functionalized with rabbit antibodies |
spellingShingle |
Synthesis, characterization and applications of maghemite beads functionalized with rabbit antibodies Rodriguez, A. F.R. flow cytometry magnetic nanobeads mossbauer spectroscopy MRI rabbit antibody XRD |
title_short |
Synthesis, characterization and applications of maghemite beads functionalized with rabbit antibodies |
title_full |
Synthesis, characterization and applications of maghemite beads functionalized with rabbit antibodies |
title_fullStr |
Synthesis, characterization and applications of maghemite beads functionalized with rabbit antibodies |
title_full_unstemmed |
Synthesis, characterization and applications of maghemite beads functionalized with rabbit antibodies |
title_sort |
Synthesis, characterization and applications of maghemite beads functionalized with rabbit antibodies |
author |
Rodriguez, A. F.R. |
author_facet |
Rodriguez, A. F.R. Rocha, C. O. [UNESP] Piazza, R. D. [UNESP] Dos Santos, C. C. [UNESP] Morales, M. A. Faria, F. S.E.D.V. Iqbal, M Zubair Barbosa, L. Chaves, Y. O. Mariuba, L. A. Jafelicci, M. [UNESP] Marques, R. F.C. [UNESP] |
author_role |
author |
author2 |
Rocha, C. O. [UNESP] Piazza, R. D. [UNESP] Dos Santos, C. C. [UNESP] Morales, M. A. Faria, F. S.E.D.V. Iqbal, M Zubair Barbosa, L. Chaves, Y. O. Mariuba, L. A. Jafelicci, M. [UNESP] Marques, R. F.C. [UNESP] |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Laboratory of Nanobiotechnology Federal University of Rio Grande Do Norte Chinese Academy of Sciences Fundacao Oswaldo Cruz Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Rodriguez, A. F.R. Rocha, C. O. [UNESP] Piazza, R. D. [UNESP] Dos Santos, C. C. [UNESP] Morales, M. A. Faria, F. S.E.D.V. Iqbal, M Zubair Barbosa, L. Chaves, Y. O. Mariuba, L. A. Jafelicci, M. [UNESP] Marques, R. F.C. [UNESP] |
dc.subject.por.fl_str_mv |
flow cytometry magnetic nanobeads mossbauer spectroscopy MRI rabbit antibody XRD |
topic |
flow cytometry magnetic nanobeads mossbauer spectroscopy MRI rabbit antibody XRD |
description |
Magnetic nanoparticles (NPs) have attracted great attention owing to their applications in the biomedical field. In the present work, maghemite (γFe2O3) NPs of 6.5 nm were prepared using a sonochemical method and used to prepare magnetic beads through silanization with 3-aminopropyltrimethoxysilane (APTS). Subsequently, amino groups in the resulting APTS-γFe2O3 beads were converted to carboxylic acid (CARB-γFe2O3) through the succinic anhydride reaction, as confirmed by transmission electron microscopy (TEM), Fourier transform infrared spectroscopy and dynamic light scattering (DLS) measurements. The size of these beads was measured as 12 nm and their hydrodynamic diameter as 490 nm, using TEM analysis and DLS, respectively. The CARB-γFe2O3 beads were further functionalized by immobilizing rabbit antibodies on their surfaces; the immobilization was confirmed by flow cytometry and ionic strength. The samples were further characterized by Mössbauer spectroscopy and DC magnetization measurements. Studies on magnetic relaxivities showed that magnetic beads present great potential for application in MR imaging. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T16:54:27Z 2018-12-11T16:54:27Z 2018-06-28 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1088/1361-6528/aacc21 Nanotechnology, v. 29, n. 36, 2018. 1361-6528 0957-4484 http://hdl.handle.net/11449/171219 10.1088/1361-6528/aacc21 2-s2.0-85049978755 2-s2.0-85049978755.pdf 2115942621694174 0000-0003-0195-3885 |
url |
http://dx.doi.org/10.1088/1361-6528/aacc21 http://hdl.handle.net/11449/171219 |
identifier_str_mv |
Nanotechnology, v. 29, n. 36, 2018. 1361-6528 0957-4484 10.1088/1361-6528/aacc21 2-s2.0-85049978755 2-s2.0-85049978755.pdf 2115942621694174 0000-0003-0195-3885 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Nanotechnology 1,079 1,079 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128403007602688 |