Synthesis, characterization and applications of maghemite beads functionalized with rabbit antibodies

Detalhes bibliográficos
Autor(a) principal: Rodriguez, A. F.R.
Data de Publicação: 2018
Outros Autores: Rocha, C. O. [UNESP], Piazza, R. D. [UNESP], Dos Santos, C. C. [UNESP], Morales, M. A., Faria, F. S.E.D.V., Iqbal, M Zubair, Barbosa, L., Chaves, Y. O., Mariuba, L. A., Jafelicci, M. [UNESP], Marques, R. F.C. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1088/1361-6528/aacc21
http://hdl.handle.net/11449/171219
Resumo: Magnetic nanoparticles (NPs) have attracted great attention owing to their applications in the biomedical field. In the present work, maghemite (γFe2O3) NPs of 6.5 nm were prepared using a sonochemical method and used to prepare magnetic beads through silanization with 3-aminopropyltrimethoxysilane (APTS). Subsequently, amino groups in the resulting APTS-γFe2O3 beads were converted to carboxylic acid (CARB-γFe2O3) through the succinic anhydride reaction, as confirmed by transmission electron microscopy (TEM), Fourier transform infrared spectroscopy and dynamic light scattering (DLS) measurements. The size of these beads was measured as 12 nm and their hydrodynamic diameter as 490 nm, using TEM analysis and DLS, respectively. The CARB-γFe2O3 beads were further functionalized by immobilizing rabbit antibodies on their surfaces; the immobilization was confirmed by flow cytometry and ionic strength. The samples were further characterized by Mössbauer spectroscopy and DC magnetization measurements. Studies on magnetic relaxivities showed that magnetic beads present great potential for application in MR imaging.
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spelling Synthesis, characterization and applications of maghemite beads functionalized with rabbit antibodiesflow cytometrymagnetic nanobeadsmossbauer spectroscopyMRIrabbit antibodyXRDMagnetic nanoparticles (NPs) have attracted great attention owing to their applications in the biomedical field. In the present work, maghemite (γFe2O3) NPs of 6.5 nm were prepared using a sonochemical method and used to prepare magnetic beads through silanization with 3-aminopropyltrimethoxysilane (APTS). Subsequently, amino groups in the resulting APTS-γFe2O3 beads were converted to carboxylic acid (CARB-γFe2O3) through the succinic anhydride reaction, as confirmed by transmission electron microscopy (TEM), Fourier transform infrared spectroscopy and dynamic light scattering (DLS) measurements. The size of these beads was measured as 12 nm and their hydrodynamic diameter as 490 nm, using TEM analysis and DLS, respectively. The CARB-γFe2O3 beads were further functionalized by immobilizing rabbit antibodies on their surfaces; the immobilization was confirmed by flow cytometry and ionic strength. The samples were further characterized by Mössbauer spectroscopy and DC magnetization measurements. Studies on magnetic relaxivities showed that magnetic beads present great potential for application in MR imaging.Federal University of Acre Laboratory of NanobiotechnologyFederal University of Rio Grande Do Norte Department of Theoretical and Experimental PhysicsDivision of Functional Materials and Nano-Devices Ningbo Institute of Materials Technology and Engineering Chinese Academy of SciencesInstituto Leonidas e Maria Deane Fundacao Oswaldo CruzSao Paulo State University - UNESP Laboratory of Magnetic Materials and Colloids Institute of Chemistry, Caixa postal 355Sao Paulo State University - UNESP Laboratory of Magnetic Materials and Colloids Institute of Chemistry, Caixa postal 355Laboratory of NanobiotechnologyFederal University of Rio Grande Do NorteChinese Academy of SciencesFundacao Oswaldo CruzUniversidade Estadual Paulista (Unesp)Rodriguez, A. F.R.Rocha, C. O. [UNESP]Piazza, R. D. [UNESP]Dos Santos, C. C. [UNESP]Morales, M. A.Faria, F. S.E.D.V.Iqbal, M ZubairBarbosa, L.Chaves, Y. O.Mariuba, L. A.Jafelicci, M. [UNESP]Marques, R. F.C. [UNESP]2018-12-11T16:54:27Z2018-12-11T16:54:27Z2018-06-28info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1088/1361-6528/aacc21Nanotechnology, v. 29, n. 36, 2018.1361-65280957-4484http://hdl.handle.net/11449/17121910.1088/1361-6528/aacc212-s2.0-850499787552-s2.0-85049978755.pdf21159426216941740000-0003-0195-3885Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengNanotechnology1,0791,079info:eu-repo/semantics/openAccess2023-10-12T06:07:19Zoai:repositorio.unesp.br:11449/171219Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:41:26.950725Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Synthesis, characterization and applications of maghemite beads functionalized with rabbit antibodies
title Synthesis, characterization and applications of maghemite beads functionalized with rabbit antibodies
spellingShingle Synthesis, characterization and applications of maghemite beads functionalized with rabbit antibodies
Rodriguez, A. F.R.
flow cytometry
magnetic nanobeads
mossbauer spectroscopy
MRI
rabbit antibody
XRD
title_short Synthesis, characterization and applications of maghemite beads functionalized with rabbit antibodies
title_full Synthesis, characterization and applications of maghemite beads functionalized with rabbit antibodies
title_fullStr Synthesis, characterization and applications of maghemite beads functionalized with rabbit antibodies
title_full_unstemmed Synthesis, characterization and applications of maghemite beads functionalized with rabbit antibodies
title_sort Synthesis, characterization and applications of maghemite beads functionalized with rabbit antibodies
author Rodriguez, A. F.R.
author_facet Rodriguez, A. F.R.
Rocha, C. O. [UNESP]
Piazza, R. D. [UNESP]
Dos Santos, C. C. [UNESP]
Morales, M. A.
Faria, F. S.E.D.V.
Iqbal, M Zubair
Barbosa, L.
Chaves, Y. O.
Mariuba, L. A.
Jafelicci, M. [UNESP]
Marques, R. F.C. [UNESP]
author_role author
author2 Rocha, C. O. [UNESP]
Piazza, R. D. [UNESP]
Dos Santos, C. C. [UNESP]
Morales, M. A.
Faria, F. S.E.D.V.
Iqbal, M Zubair
Barbosa, L.
Chaves, Y. O.
Mariuba, L. A.
Jafelicci, M. [UNESP]
Marques, R. F.C. [UNESP]
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Laboratory of Nanobiotechnology
Federal University of Rio Grande Do Norte
Chinese Academy of Sciences
Fundacao Oswaldo Cruz
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Rodriguez, A. F.R.
Rocha, C. O. [UNESP]
Piazza, R. D. [UNESP]
Dos Santos, C. C. [UNESP]
Morales, M. A.
Faria, F. S.E.D.V.
Iqbal, M Zubair
Barbosa, L.
Chaves, Y. O.
Mariuba, L. A.
Jafelicci, M. [UNESP]
Marques, R. F.C. [UNESP]
dc.subject.por.fl_str_mv flow cytometry
magnetic nanobeads
mossbauer spectroscopy
MRI
rabbit antibody
XRD
topic flow cytometry
magnetic nanobeads
mossbauer spectroscopy
MRI
rabbit antibody
XRD
description Magnetic nanoparticles (NPs) have attracted great attention owing to their applications in the biomedical field. In the present work, maghemite (γFe2O3) NPs of 6.5 nm were prepared using a sonochemical method and used to prepare magnetic beads through silanization with 3-aminopropyltrimethoxysilane (APTS). Subsequently, amino groups in the resulting APTS-γFe2O3 beads were converted to carboxylic acid (CARB-γFe2O3) through the succinic anhydride reaction, as confirmed by transmission electron microscopy (TEM), Fourier transform infrared spectroscopy and dynamic light scattering (DLS) measurements. The size of these beads was measured as 12 nm and their hydrodynamic diameter as 490 nm, using TEM analysis and DLS, respectively. The CARB-γFe2O3 beads were further functionalized by immobilizing rabbit antibodies on their surfaces; the immobilization was confirmed by flow cytometry and ionic strength. The samples were further characterized by Mössbauer spectroscopy and DC magnetization measurements. Studies on magnetic relaxivities showed that magnetic beads present great potential for application in MR imaging.
publishDate 2018
dc.date.none.fl_str_mv 2018-12-11T16:54:27Z
2018-12-11T16:54:27Z
2018-06-28
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1088/1361-6528/aacc21
Nanotechnology, v. 29, n. 36, 2018.
1361-6528
0957-4484
http://hdl.handle.net/11449/171219
10.1088/1361-6528/aacc21
2-s2.0-85049978755
2-s2.0-85049978755.pdf
2115942621694174
0000-0003-0195-3885
url http://dx.doi.org/10.1088/1361-6528/aacc21
http://hdl.handle.net/11449/171219
identifier_str_mv Nanotechnology, v. 29, n. 36, 2018.
1361-6528
0957-4484
10.1088/1361-6528/aacc21
2-s2.0-85049978755
2-s2.0-85049978755.pdf
2115942621694174
0000-0003-0195-3885
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Nanotechnology
1,079
1,079
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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