Avaliação in vitro da obliteração de túbulos dentinários utilizando dentifrícios contendo trimetafosfato de sódio

• Autor(a) principal: Toledo, Priscila Toninatto Alves de [UNESP]
• Data de Publicação: 2017
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/156749; http://www.athena.biblioteca.unesp.br/exlibris/bd/capelo/2018-06-12/000901973.pdf
• topic: Dentina; Dentifrícios; Fosfatos; Nanopartículas; Dentin

The objective of this study was to evaluate in vitro the effect of TMP microparticles (TMPm) or nanoparticles (TMPn) associated with fluoride (F) in toothpaste formulations on the obliteration of dentinal tubules (DT). Bovine root dentin blocks (4×4×2 mm) were ground flat and the DT were unobstructed by phosphoric acid 37%. Blocks (n=20/group) were submitted to mechanical brushing (2x/day) during 7 days with toothpastes: placebo (without F and TMP), 1100 ppm F (1100), 1100 ppm F associated with 3% TMPm (1100 TMPm), 1100 ppm F associated with 3% TMPn (1100 TMPn). After that, half of the blocks were immersed in citric acid (pH 3.2) for 1 minute. The number, diameter and area of unobstructed DT were quantified using scanning electron microscopy (SEM) and atomic percentage (%) of chemical elements (Ca, P, C, N and O) from precipitates in DT were determined by energy-dispersive X-ray. Data were submitted to 2-way ANOVA, followed by Student-Newman-Keuls test (p<0.05). Toohtpastes containing TMPm and TMPn produced greater obliteration of DT (p<0,05). Higher Ca/P ratios were observed in the groups treated with TMP, whereas the acid challenge reduced the Ca/P ratio for all groups. The atomic % of C and N was significantly lower for TMP toothpastes, increasing this percentage after the acid challenge. It was concluded that the addition of TMP to toothpastes containing 1100 ppm F produced significantly greater obliteration of the dentinal tubules when compared to 1100 ppm F toothpaste and the size of particles of TMP (micro or nano) had no effect on the obliteration of dentinal tubules