Plasma rico em plaquetas: Estudo comparativo entre a dupla centrifugação e o filtro E-PET (Equine Platelet Enhancement Therapy)
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S0100-736X2017000300003 http://hdl.handle.net/11449/174779 |
Resumo: | The platelet-rich plasma (PRP) consists of a high concentration of platelets in a small volume of plasma, four times greater (average) than the serum concentration. The use of PRP is justified by the high concentration of growth factors present in granules in the platelets, which have several functions such as cell proliferation, chemotaxis, angiogenesis and differenciation, which extend the power of tissue repair. There are several protocols to obtain PRP in horses described in the literature, among which are highlighted the double centrifugation, automated and filters. There are substantial differences in the PRP content depending on its method of production, especially when it concerns the amount of leukocytes, platelets and concentration of growth factors. Thus, the aim of this study was to compare the use of platelet concentrates obtained by double centrifugation protocol and obtained by the filter E-PET (Equine Platelet Enhancement Therapy) taking into account the platelet and leukocyte final concentration, the quantification of growth factors (TGFß and PDGF-BB) and the facility to do those methods. Nine horses were used to obtain PRP by double centrifugation and through the E-PET filter, with no statistical difference (p > 0.05) between the methods relative to the platelet and leukocyte concentration; however, there was statistical difference (p = 0.002 and p = 0.004 respectively) compared with the concentration of TGFß and PDGF-BB. It was concluded that the E-PET filter proved to be a more effective method possible to use in the field and to provide a higher concentration of growth factors (TGFß and PDGF-BB). |
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Plasma rico em plaquetas: Estudo comparativo entre a dupla centrifugação e o filtro E-PET (Equine Platelet Enhancement Therapy)Comparative study of double spin and use of E-PET filter (Equine Platelet Enhancement Therapy) to obtain platelet rich plasma in horsesThe platelet-rich plasma (PRP) consists of a high concentration of platelets in a small volume of plasma, four times greater (average) than the serum concentration. The use of PRP is justified by the high concentration of growth factors present in granules in the platelets, which have several functions such as cell proliferation, chemotaxis, angiogenesis and differenciation, which extend the power of tissue repair. There are several protocols to obtain PRP in horses described in the literature, among which are highlighted the double centrifugation, automated and filters. There are substantial differences in the PRP content depending on its method of production, especially when it concerns the amount of leukocytes, platelets and concentration of growth factors. Thus, the aim of this study was to compare the use of platelet concentrates obtained by double centrifugation protocol and obtained by the filter E-PET (Equine Platelet Enhancement Therapy) taking into account the platelet and leukocyte final concentration, the quantification of growth factors (TGFß and PDGF-BB) and the facility to do those methods. Nine horses were used to obtain PRP by double centrifugation and through the E-PET filter, with no statistical difference (p > 0.05) between the methods relative to the platelet and leukocyte concentration; however, there was statistical difference (p = 0.002 and p = 0.004 respectively) compared with the concentration of TGFß and PDGF-BB. It was concluded that the E-PET filter proved to be a more effective method possible to use in the field and to provide a higher concentration of growth factors (TGFß and PDGF-BB).Departamento de Cirurgia e Anestesiologia Veterinária Faculdade de Medicina Veterinária e Zootecnia Campus de Botucatu Universidade Estadual Paulista 'Júlio de Mesquita Filho' (Unesp), Distrito de Rubião Júnior s/nDepartamento de Cirurgia e Anestesiologia Veterinária Faculdade de Medicina Veterinária e Zootecnia Campus de Botucatu Universidade Estadual Paulista 'Júlio de Mesquita Filho' (Unesp), Distrito de Rubião Júnior s/nUniversidade Estadual Paulista (Unesp)Conceição, Mariana L. [UNESP]Alvarenga, Marina L. [UNESP]Souza, Jaqueline B. [UNESP]Mattos, Luiz Henrique L. [UNESP]Rodrigues, Celso A. [UNESP]Watanabe, Marcos J. [UNESP]Hussni, Carlos Alberto [UNESP]Alves, Ana Liz G. [UNESP]2018-12-11T17:12:49Z2018-12-11T17:12:49Z2017-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article215-220application/pdfhttp://dx.doi.org/10.1590/S0100-736X2017000300003Pesquisa Veterinaria Brasileira, v. 37, n. 3, p. 215-220, 2017.1678-51500100-736Xhttp://hdl.handle.net/11449/17477910.1590/S0100-736X2017000300003S0100-736X20170003002152-s2.0-85021046254S0100-736X2017000300215.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengporPesquisa Veterinaria Brasileirainfo:eu-repo/semantics/openAccess2024-01-13T06:36:06Zoai:repositorio.unesp.br:11449/174779Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-13T06:36:06Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Plasma rico em plaquetas: Estudo comparativo entre a dupla centrifugação e o filtro E-PET (Equine Platelet Enhancement Therapy) Comparative study of double spin and use of E-PET filter (Equine Platelet Enhancement Therapy) to obtain platelet rich plasma in horses |
title |
Plasma rico em plaquetas: Estudo comparativo entre a dupla centrifugação e o filtro E-PET (Equine Platelet Enhancement Therapy) |
spellingShingle |
Plasma rico em plaquetas: Estudo comparativo entre a dupla centrifugação e o filtro E-PET (Equine Platelet Enhancement Therapy) Conceição, Mariana L. [UNESP] |
title_short |
Plasma rico em plaquetas: Estudo comparativo entre a dupla centrifugação e o filtro E-PET (Equine Platelet Enhancement Therapy) |
title_full |
Plasma rico em plaquetas: Estudo comparativo entre a dupla centrifugação e o filtro E-PET (Equine Platelet Enhancement Therapy) |
title_fullStr |
Plasma rico em plaquetas: Estudo comparativo entre a dupla centrifugação e o filtro E-PET (Equine Platelet Enhancement Therapy) |
title_full_unstemmed |
Plasma rico em plaquetas: Estudo comparativo entre a dupla centrifugação e o filtro E-PET (Equine Platelet Enhancement Therapy) |
title_sort |
Plasma rico em plaquetas: Estudo comparativo entre a dupla centrifugação e o filtro E-PET (Equine Platelet Enhancement Therapy) |
author |
Conceição, Mariana L. [UNESP] |
author_facet |
Conceição, Mariana L. [UNESP] Alvarenga, Marina L. [UNESP] Souza, Jaqueline B. [UNESP] Mattos, Luiz Henrique L. [UNESP] Rodrigues, Celso A. [UNESP] Watanabe, Marcos J. [UNESP] Hussni, Carlos Alberto [UNESP] Alves, Ana Liz G. [UNESP] |
author_role |
author |
author2 |
Alvarenga, Marina L. [UNESP] Souza, Jaqueline B. [UNESP] Mattos, Luiz Henrique L. [UNESP] Rodrigues, Celso A. [UNESP] Watanabe, Marcos J. [UNESP] Hussni, Carlos Alberto [UNESP] Alves, Ana Liz G. [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Conceição, Mariana L. [UNESP] Alvarenga, Marina L. [UNESP] Souza, Jaqueline B. [UNESP] Mattos, Luiz Henrique L. [UNESP] Rodrigues, Celso A. [UNESP] Watanabe, Marcos J. [UNESP] Hussni, Carlos Alberto [UNESP] Alves, Ana Liz G. [UNESP] |
description |
The platelet-rich plasma (PRP) consists of a high concentration of platelets in a small volume of plasma, four times greater (average) than the serum concentration. The use of PRP is justified by the high concentration of growth factors present in granules in the platelets, which have several functions such as cell proliferation, chemotaxis, angiogenesis and differenciation, which extend the power of tissue repair. There are several protocols to obtain PRP in horses described in the literature, among which are highlighted the double centrifugation, automated and filters. There are substantial differences in the PRP content depending on its method of production, especially when it concerns the amount of leukocytes, platelets and concentration of growth factors. Thus, the aim of this study was to compare the use of platelet concentrates obtained by double centrifugation protocol and obtained by the filter E-PET (Equine Platelet Enhancement Therapy) taking into account the platelet and leukocyte final concentration, the quantification of growth factors (TGFß and PDGF-BB) and the facility to do those methods. Nine horses were used to obtain PRP by double centrifugation and through the E-PET filter, with no statistical difference (p > 0.05) between the methods relative to the platelet and leukocyte concentration; however, there was statistical difference (p = 0.002 and p = 0.004 respectively) compared with the concentration of TGFß and PDGF-BB. It was concluded that the E-PET filter proved to be a more effective method possible to use in the field and to provide a higher concentration of growth factors (TGFß and PDGF-BB). |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-03-01 2018-12-11T17:12:49Z 2018-12-11T17:12:49Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0100-736X2017000300003 Pesquisa Veterinaria Brasileira, v. 37, n. 3, p. 215-220, 2017. 1678-5150 0100-736X http://hdl.handle.net/11449/174779 10.1590/S0100-736X2017000300003 S0100-736X2017000300215 2-s2.0-85021046254 S0100-736X2017000300215.pdf |
url |
http://dx.doi.org/10.1590/S0100-736X2017000300003 http://hdl.handle.net/11449/174779 |
identifier_str_mv |
Pesquisa Veterinaria Brasileira, v. 37, n. 3, p. 215-220, 2017. 1678-5150 0100-736X 10.1590/S0100-736X2017000300003 S0100-736X2017000300215 2-s2.0-85021046254 S0100-736X2017000300215.pdf |
dc.language.iso.fl_str_mv |
eng por |
language |
eng por |
dc.relation.none.fl_str_mv |
Pesquisa Veterinaria Brasileira |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
215-220 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803046686647386112 |