Promising effects of silver tungstate microcrystals on fibroblast human cells and three dimensional collagen matrix models: A novel non-cytotoxic material to fight oral disease
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.colsurfb.2018.06.023 http://hdl.handle.net/11449/176505 |
Resumo: | Silver tungstate (α-Ag2WO4) microcrystals have shown encouraging results regarding their antimicrobial activity. However, in addition to the promising outcomes in fighting oral disease, cytotoxic tests are mandatory for screening new materials for biological applications. Here, we developed a better understanding of the effects of microcrystals on the behavior of both human gingival fibroblast (HGF) cells and three-dimensional (3D) collagen matrices. To perform these experiments, the lowest concentration of α-Ag2WO4 capable of preventing the visible growth of Candida albicans (C. albicans) planktonic cells was defined as the test concentration, and it ranged from 0.781 (C1) to 7.81 (C2) to 78.1 (C3) μg/mL. Complete medium and lysis buffer (LB) served as negative (C−) and positive (C+) controls, respectively. The effect of the microcrystal concentration on the morphology, remodeling and proliferation of HGF cells was evaluated by different approaches. Quantitative and qualitative assessments demonstrated that α-Ag2WO4 did not affect the mitochondrial enzymatic activity of HGF cells cultured in a monolayer or the cell viability within 3D collagen matrices. These experiments showed that α-Ag2WO4 at the C2 concentration did not damage the genomic DNA. The development of new materials is attractive for the possible treatment of diseases and for avoiding indiscriminate prescribing of antibiotics. These findings provide information on the effect of α-Ag2WO4 on cell behavior and reveal that these microcrystals are non-cytotoxic against human gingival cells over a sufficient period to measure the hazard potential. |
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Promising effects of silver tungstate microcrystals on fibroblast human cells and three dimensional collagen matrix models: A novel non-cytotoxic material to fight oral diseaseCytotoxicityFibroblastMicrocrystalsSilverSilver tungstate (α-Ag2WO4) microcrystals have shown encouraging results regarding their antimicrobial activity. However, in addition to the promising outcomes in fighting oral disease, cytotoxic tests are mandatory for screening new materials for biological applications. Here, we developed a better understanding of the effects of microcrystals on the behavior of both human gingival fibroblast (HGF) cells and three-dimensional (3D) collagen matrices. To perform these experiments, the lowest concentration of α-Ag2WO4 capable of preventing the visible growth of Candida albicans (C. albicans) planktonic cells was defined as the test concentration, and it ranged from 0.781 (C1) to 7.81 (C2) to 78.1 (C3) μg/mL. Complete medium and lysis buffer (LB) served as negative (C−) and positive (C+) controls, respectively. The effect of the microcrystal concentration on the morphology, remodeling and proliferation of HGF cells was evaluated by different approaches. Quantitative and qualitative assessments demonstrated that α-Ag2WO4 did not affect the mitochondrial enzymatic activity of HGF cells cultured in a monolayer or the cell viability within 3D collagen matrices. These experiments showed that α-Ag2WO4 at the C2 concentration did not damage the genomic DNA. The development of new materials is attractive for the possible treatment of diseases and for avoiding indiscriminate prescribing of antibiotics. These findings provide information on the effect of α-Ag2WO4 on cell behavior and reveal that these microcrystals are non-cytotoxic against human gingival cells over a sufficient period to measure the hazard potential.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Dental Materials and Prosthodontics School of Dentistry at Araraquara São Paulo State University (UNESP), Rua Humaita, 1680CDMF-UFSCar-Universidade Federal de São Carlos, Washington Luis km 235, P.O. Box 676Department of Dental Materials and Prosthodontics School of Dentistry at Araraquara São Paulo State University (UNESP), Rua Humaita, 1680CNPq: #163196/2015-0FAPESP: #2015/03567-7FAPESP: #2015/03654-7FAPESP: #2015/25124-0Universidade Estadual Paulista (Unesp)Universidade Federal de São Carlos (UFSCar)Haro Chávez, Natali Lisette [UNESP]de Avila, Erica Dorigatti [UNESP]Barbugli, Paula Aboud [UNESP]de Oliveira, Regiane Cristinade Foggi, Camila Cristina [UNESP]Longo, ElsonVergani, Carlos Eduardo [UNESP]2018-12-11T17:21:03Z2018-12-11T17:21:03Z2018-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article505-513application/pdfhttp://dx.doi.org/10.1016/j.colsurfb.2018.06.023Colloids and Surfaces B: Biointerfaces, v. 170, p. 505-513.1873-43670927-7765http://hdl.handle.net/11449/17650510.1016/j.colsurfb.2018.06.0232-s2.0-850490939092-s2.0-85049093909.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengColloids and Surfaces B: Biointerfaces1,071info:eu-repo/semantics/openAccess2024-09-27T14:56:22Zoai:repositorio.unesp.br:11449/176505Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-27T14:56:22Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Promising effects of silver tungstate microcrystals on fibroblast human cells and three dimensional collagen matrix models: A novel non-cytotoxic material to fight oral disease |
title |
Promising effects of silver tungstate microcrystals on fibroblast human cells and three dimensional collagen matrix models: A novel non-cytotoxic material to fight oral disease |
spellingShingle |
Promising effects of silver tungstate microcrystals on fibroblast human cells and three dimensional collagen matrix models: A novel non-cytotoxic material to fight oral disease Haro Chávez, Natali Lisette [UNESP] Cytotoxicity Fibroblast Microcrystals Silver |
title_short |
Promising effects of silver tungstate microcrystals on fibroblast human cells and three dimensional collagen matrix models: A novel non-cytotoxic material to fight oral disease |
title_full |
Promising effects of silver tungstate microcrystals on fibroblast human cells and three dimensional collagen matrix models: A novel non-cytotoxic material to fight oral disease |
title_fullStr |
Promising effects of silver tungstate microcrystals on fibroblast human cells and three dimensional collagen matrix models: A novel non-cytotoxic material to fight oral disease |
title_full_unstemmed |
Promising effects of silver tungstate microcrystals on fibroblast human cells and three dimensional collagen matrix models: A novel non-cytotoxic material to fight oral disease |
title_sort |
Promising effects of silver tungstate microcrystals on fibroblast human cells and three dimensional collagen matrix models: A novel non-cytotoxic material to fight oral disease |
author |
Haro Chávez, Natali Lisette [UNESP] |
author_facet |
Haro Chávez, Natali Lisette [UNESP] de Avila, Erica Dorigatti [UNESP] Barbugli, Paula Aboud [UNESP] de Oliveira, Regiane Cristina de Foggi, Camila Cristina [UNESP] Longo, Elson Vergani, Carlos Eduardo [UNESP] |
author_role |
author |
author2 |
de Avila, Erica Dorigatti [UNESP] Barbugli, Paula Aboud [UNESP] de Oliveira, Regiane Cristina de Foggi, Camila Cristina [UNESP] Longo, Elson Vergani, Carlos Eduardo [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Federal de São Carlos (UFSCar) |
dc.contributor.author.fl_str_mv |
Haro Chávez, Natali Lisette [UNESP] de Avila, Erica Dorigatti [UNESP] Barbugli, Paula Aboud [UNESP] de Oliveira, Regiane Cristina de Foggi, Camila Cristina [UNESP] Longo, Elson Vergani, Carlos Eduardo [UNESP] |
dc.subject.por.fl_str_mv |
Cytotoxicity Fibroblast Microcrystals Silver |
topic |
Cytotoxicity Fibroblast Microcrystals Silver |
description |
Silver tungstate (α-Ag2WO4) microcrystals have shown encouraging results regarding their antimicrobial activity. However, in addition to the promising outcomes in fighting oral disease, cytotoxic tests are mandatory for screening new materials for biological applications. Here, we developed a better understanding of the effects of microcrystals on the behavior of both human gingival fibroblast (HGF) cells and three-dimensional (3D) collagen matrices. To perform these experiments, the lowest concentration of α-Ag2WO4 capable of preventing the visible growth of Candida albicans (C. albicans) planktonic cells was defined as the test concentration, and it ranged from 0.781 (C1) to 7.81 (C2) to 78.1 (C3) μg/mL. Complete medium and lysis buffer (LB) served as negative (C−) and positive (C+) controls, respectively. The effect of the microcrystal concentration on the morphology, remodeling and proliferation of HGF cells was evaluated by different approaches. Quantitative and qualitative assessments demonstrated that α-Ag2WO4 did not affect the mitochondrial enzymatic activity of HGF cells cultured in a monolayer or the cell viability within 3D collagen matrices. These experiments showed that α-Ag2WO4 at the C2 concentration did not damage the genomic DNA. The development of new materials is attractive for the possible treatment of diseases and for avoiding indiscriminate prescribing of antibiotics. These findings provide information on the effect of α-Ag2WO4 on cell behavior and reveal that these microcrystals are non-cytotoxic against human gingival cells over a sufficient period to measure the hazard potential. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T17:21:03Z 2018-12-11T17:21:03Z 2018-10-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.colsurfb.2018.06.023 Colloids and Surfaces B: Biointerfaces, v. 170, p. 505-513. 1873-4367 0927-7765 http://hdl.handle.net/11449/176505 10.1016/j.colsurfb.2018.06.023 2-s2.0-85049093909 2-s2.0-85049093909.pdf |
url |
http://dx.doi.org/10.1016/j.colsurfb.2018.06.023 http://hdl.handle.net/11449/176505 |
identifier_str_mv |
Colloids and Surfaces B: Biointerfaces, v. 170, p. 505-513. 1873-4367 0927-7765 10.1016/j.colsurfb.2018.06.023 2-s2.0-85049093909 2-s2.0-85049093909.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Colloids and Surfaces B: Biointerfaces 1,071 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
505-513 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
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1813546383677849600 |