Promising effects of silver tungstate microcrystals on fibroblast human cells and three dimensional collagen matrix models: A novel non-cytotoxic material to fight oral disease

Detalhes bibliográficos
Autor(a) principal: Haro Chávez, Natali Lisette [UNESP]
Data de Publicação: 2018
Outros Autores: de Avila, Erica Dorigatti [UNESP], Barbugli, Paula Aboud [UNESP], de Oliveira, Regiane Cristina, de Foggi, Camila Cristina [UNESP], Longo, Elson, Vergani, Carlos Eduardo [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.colsurfb.2018.06.023
http://hdl.handle.net/11449/176505
Resumo: Silver tungstate (α-Ag2WO4) microcrystals have shown encouraging results regarding their antimicrobial activity. However, in addition to the promising outcomes in fighting oral disease, cytotoxic tests are mandatory for screening new materials for biological applications. Here, we developed a better understanding of the effects of microcrystals on the behavior of both human gingival fibroblast (HGF) cells and three-dimensional (3D) collagen matrices. To perform these experiments, the lowest concentration of α-Ag2WO4 capable of preventing the visible growth of Candida albicans (C. albicans) planktonic cells was defined as the test concentration, and it ranged from 0.781 (C1) to 7.81 (C2) to 78.1 (C3) μg/mL. Complete medium and lysis buffer (LB) served as negative (C−) and positive (C+) controls, respectively. The effect of the microcrystal concentration on the morphology, remodeling and proliferation of HGF cells was evaluated by different approaches. Quantitative and qualitative assessments demonstrated that α-Ag2WO4 did not affect the mitochondrial enzymatic activity of HGF cells cultured in a monolayer or the cell viability within 3D collagen matrices. These experiments showed that α-Ag2WO4 at the C2 concentration did not damage the genomic DNA. The development of new materials is attractive for the possible treatment of diseases and for avoiding indiscriminate prescribing of antibiotics. These findings provide information on the effect of α-Ag2WO4 on cell behavior and reveal that these microcrystals are non-cytotoxic against human gingival cells over a sufficient period to measure the hazard potential.
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spelling Promising effects of silver tungstate microcrystals on fibroblast human cells and three dimensional collagen matrix models: A novel non-cytotoxic material to fight oral diseaseCytotoxicityFibroblastMicrocrystalsSilverSilver tungstate (α-Ag2WO4) microcrystals have shown encouraging results regarding their antimicrobial activity. However, in addition to the promising outcomes in fighting oral disease, cytotoxic tests are mandatory for screening new materials for biological applications. Here, we developed a better understanding of the effects of microcrystals on the behavior of both human gingival fibroblast (HGF) cells and three-dimensional (3D) collagen matrices. To perform these experiments, the lowest concentration of α-Ag2WO4 capable of preventing the visible growth of Candida albicans (C. albicans) planktonic cells was defined as the test concentration, and it ranged from 0.781 (C1) to 7.81 (C2) to 78.1 (C3) μg/mL. Complete medium and lysis buffer (LB) served as negative (C−) and positive (C+) controls, respectively. The effect of the microcrystal concentration on the morphology, remodeling and proliferation of HGF cells was evaluated by different approaches. Quantitative and qualitative assessments demonstrated that α-Ag2WO4 did not affect the mitochondrial enzymatic activity of HGF cells cultured in a monolayer or the cell viability within 3D collagen matrices. These experiments showed that α-Ag2WO4 at the C2 concentration did not damage the genomic DNA. The development of new materials is attractive for the possible treatment of diseases and for avoiding indiscriminate prescribing of antibiotics. These findings provide information on the effect of α-Ag2WO4 on cell behavior and reveal that these microcrystals are non-cytotoxic against human gingival cells over a sufficient period to measure the hazard potential.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Dental Materials and Prosthodontics School of Dentistry at Araraquara São Paulo State University (UNESP), Rua Humaita, 1680CDMF-UFSCar-Universidade Federal de São Carlos, Washington Luis km 235, P.O. Box 676Department of Dental Materials and Prosthodontics School of Dentistry at Araraquara São Paulo State University (UNESP), Rua Humaita, 1680CNPq: #163196/2015-0FAPESP: #2015/03567-7FAPESP: #2015/03654-7FAPESP: #2015/25124-0Universidade Estadual Paulista (Unesp)Universidade Federal de São Carlos (UFSCar)Haro Chávez, Natali Lisette [UNESP]de Avila, Erica Dorigatti [UNESP]Barbugli, Paula Aboud [UNESP]de Oliveira, Regiane Cristinade Foggi, Camila Cristina [UNESP]Longo, ElsonVergani, Carlos Eduardo [UNESP]2018-12-11T17:21:03Z2018-12-11T17:21:03Z2018-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article505-513application/pdfhttp://dx.doi.org/10.1016/j.colsurfb.2018.06.023Colloids and Surfaces B: Biointerfaces, v. 170, p. 505-513.1873-43670927-7765http://hdl.handle.net/11449/17650510.1016/j.colsurfb.2018.06.0232-s2.0-850490939092-s2.0-85049093909.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengColloids and Surfaces B: Biointerfaces1,071info:eu-repo/semantics/openAccess2024-09-27T14:56:22Zoai:repositorio.unesp.br:11449/176505Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-27T14:56:22Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Promising effects of silver tungstate microcrystals on fibroblast human cells and three dimensional collagen matrix models: A novel non-cytotoxic material to fight oral disease
title Promising effects of silver tungstate microcrystals on fibroblast human cells and three dimensional collagen matrix models: A novel non-cytotoxic material to fight oral disease
spellingShingle Promising effects of silver tungstate microcrystals on fibroblast human cells and three dimensional collagen matrix models: A novel non-cytotoxic material to fight oral disease
Haro Chávez, Natali Lisette [UNESP]
Cytotoxicity
Fibroblast
Microcrystals
Silver
title_short Promising effects of silver tungstate microcrystals on fibroblast human cells and three dimensional collagen matrix models: A novel non-cytotoxic material to fight oral disease
title_full Promising effects of silver tungstate microcrystals on fibroblast human cells and three dimensional collagen matrix models: A novel non-cytotoxic material to fight oral disease
title_fullStr Promising effects of silver tungstate microcrystals on fibroblast human cells and three dimensional collagen matrix models: A novel non-cytotoxic material to fight oral disease
title_full_unstemmed Promising effects of silver tungstate microcrystals on fibroblast human cells and three dimensional collagen matrix models: A novel non-cytotoxic material to fight oral disease
title_sort Promising effects of silver tungstate microcrystals on fibroblast human cells and three dimensional collagen matrix models: A novel non-cytotoxic material to fight oral disease
author Haro Chávez, Natali Lisette [UNESP]
author_facet Haro Chávez, Natali Lisette [UNESP]
de Avila, Erica Dorigatti [UNESP]
Barbugli, Paula Aboud [UNESP]
de Oliveira, Regiane Cristina
de Foggi, Camila Cristina [UNESP]
Longo, Elson
Vergani, Carlos Eduardo [UNESP]
author_role author
author2 de Avila, Erica Dorigatti [UNESP]
Barbugli, Paula Aboud [UNESP]
de Oliveira, Regiane Cristina
de Foggi, Camila Cristina [UNESP]
Longo, Elson
Vergani, Carlos Eduardo [UNESP]
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade Federal de São Carlos (UFSCar)
dc.contributor.author.fl_str_mv Haro Chávez, Natali Lisette [UNESP]
de Avila, Erica Dorigatti [UNESP]
Barbugli, Paula Aboud [UNESP]
de Oliveira, Regiane Cristina
de Foggi, Camila Cristina [UNESP]
Longo, Elson
Vergani, Carlos Eduardo [UNESP]
dc.subject.por.fl_str_mv Cytotoxicity
Fibroblast
Microcrystals
Silver
topic Cytotoxicity
Fibroblast
Microcrystals
Silver
description Silver tungstate (α-Ag2WO4) microcrystals have shown encouraging results regarding their antimicrobial activity. However, in addition to the promising outcomes in fighting oral disease, cytotoxic tests are mandatory for screening new materials for biological applications. Here, we developed a better understanding of the effects of microcrystals on the behavior of both human gingival fibroblast (HGF) cells and three-dimensional (3D) collagen matrices. To perform these experiments, the lowest concentration of α-Ag2WO4 capable of preventing the visible growth of Candida albicans (C. albicans) planktonic cells was defined as the test concentration, and it ranged from 0.781 (C1) to 7.81 (C2) to 78.1 (C3) μg/mL. Complete medium and lysis buffer (LB) served as negative (C−) and positive (C+) controls, respectively. The effect of the microcrystal concentration on the morphology, remodeling and proliferation of HGF cells was evaluated by different approaches. Quantitative and qualitative assessments demonstrated that α-Ag2WO4 did not affect the mitochondrial enzymatic activity of HGF cells cultured in a monolayer or the cell viability within 3D collagen matrices. These experiments showed that α-Ag2WO4 at the C2 concentration did not damage the genomic DNA. The development of new materials is attractive for the possible treatment of diseases and for avoiding indiscriminate prescribing of antibiotics. These findings provide information on the effect of α-Ag2WO4 on cell behavior and reveal that these microcrystals are non-cytotoxic against human gingival cells over a sufficient period to measure the hazard potential.
publishDate 2018
dc.date.none.fl_str_mv 2018-12-11T17:21:03Z
2018-12-11T17:21:03Z
2018-10-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.colsurfb.2018.06.023
Colloids and Surfaces B: Biointerfaces, v. 170, p. 505-513.
1873-4367
0927-7765
http://hdl.handle.net/11449/176505
10.1016/j.colsurfb.2018.06.023
2-s2.0-85049093909
2-s2.0-85049093909.pdf
url http://dx.doi.org/10.1016/j.colsurfb.2018.06.023
http://hdl.handle.net/11449/176505
identifier_str_mv Colloids and Surfaces B: Biointerfaces, v. 170, p. 505-513.
1873-4367
0927-7765
10.1016/j.colsurfb.2018.06.023
2-s2.0-85049093909
2-s2.0-85049093909.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Colloids and Surfaces B: Biointerfaces
1,071
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 505-513
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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