Pesticide dichorvos induces early solid Ehrlich tumoral development associated with a non-protective pro-inflammatory response
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s11356-017-1104-x http://hdl.handle.net/11449/175697 |
Resumo: | Prolonged exposure to dichlorvos (DDVP), a common pesticide used for food crops, has been related to the development of infections and malignancies. Macrophages are used as bioindicators of immunotoxicity; thus, evaluation of their activity in solid Ehrlich tumor-bearing mice (TBM) may be useful to evaluate the influence of pesticides on human health. To investigate the effects of low DDVP doses, Swiss mice were divided into the following groups: the DDVP group, composed of mice fed diets containing 10 mg/kg of DDVP; the TBM group, consisting of mice subcutaneously inoculated with 107 tumor cells/100 μl and fed a basal diet; the DDVP-TBM group, consisting of mice previously fed DDVP-containing diets for 28 days and then subcutaneously inoculated with tumor cells; and the control (CTRL) group, composed of mice fed a basal diet. After 7 and 21 days of tumor inoculation, the mice were euthanized; and after necroscopic examination, the neoplastic mass, organs, and intraperitoneal fluid were collected. Adherent peritoneal cells were cultivated to determine the production of H2O2 and TNF. Altogether, our results indicate that even at low doses, the intake of DDVP caused weight loss and increased tumor mass, which were associated with H2O2 production and high levels of TNF, a pro-inflammatory cytokine. These data are important as the exposure to pesticides, even at low doses, could potentially hinder the immune response against tumors and, consequently, create favorable conditions for their development. |
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Pesticide dichorvos induces early solid Ehrlich tumoral development associated with a non-protective pro-inflammatory responseCytokinesDichorvosEhrlich tumorImmunotoxicityMacrophage activityOrganophosphorusProlonged exposure to dichlorvos (DDVP), a common pesticide used for food crops, has been related to the development of infections and malignancies. Macrophages are used as bioindicators of immunotoxicity; thus, evaluation of their activity in solid Ehrlich tumor-bearing mice (TBM) may be useful to evaluate the influence of pesticides on human health. To investigate the effects of low DDVP doses, Swiss mice were divided into the following groups: the DDVP group, composed of mice fed diets containing 10 mg/kg of DDVP; the TBM group, consisting of mice subcutaneously inoculated with 107 tumor cells/100 μl and fed a basal diet; the DDVP-TBM group, consisting of mice previously fed DDVP-containing diets for 28 days and then subcutaneously inoculated with tumor cells; and the control (CTRL) group, composed of mice fed a basal diet. After 7 and 21 days of tumor inoculation, the mice were euthanized; and after necroscopic examination, the neoplastic mass, organs, and intraperitoneal fluid were collected. Adherent peritoneal cells were cultivated to determine the production of H2O2 and TNF. Altogether, our results indicate that even at low doses, the intake of DDVP caused weight loss and increased tumor mass, which were associated with H2O2 production and high levels of TNF, a pro-inflammatory cytokine. These data are important as the exposure to pesticides, even at low doses, could potentially hinder the immune response against tumors and, consequently, create favorable conditions for their development.Experimental Immunopathology Laboratory Department of Biological Sciences College of Sciences Universidade Estadual Paulista Julio de Mesquita Filho – UNESPDepartment of Pathology Botucatu Medical School Universidade Estadual Paulista Julio de Mesquita Filho – UNESPExperimental Immunopathology Laboratory Department of Biological Sciences College of Sciences Universidade Estadual Paulista Julio de Mesquita Filho – UNESPDepartment of Pathology Botucatu Medical School Universidade Estadual Paulista Julio de Mesquita Filho – UNESPUniversidade Estadual Paulista (Unesp)de Camargo, Marcela Rodrigues [UNESP]Akamine, Pedro Vinicius Teruya [UNESP]Venturini, James [UNESP]de Arruda, Maria Sueli P. [UNESP]da Silva Franchi, Carla Adriene [UNESP]2018-12-11T17:17:07Z2018-12-11T17:17:07Z2018-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article7681-7687application/pdfhttp://dx.doi.org/10.1007/s11356-017-1104-xEnvironmental Science and Pollution Research, v. 25, n. 8, p. 7681-7687, 2018.1614-74990944-1344http://hdl.handle.net/11449/17569710.1007/s11356-017-1104-x2-s2.0-850395529852-s2.0-85039552985.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEnvironmental Science and Pollution Research0,858info:eu-repo/semantics/openAccess2024-09-03T13:15:29Zoai:repositorio.unesp.br:11449/175697Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-03T13:15:29Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Pesticide dichorvos induces early solid Ehrlich tumoral development associated with a non-protective pro-inflammatory response |
title |
Pesticide dichorvos induces early solid Ehrlich tumoral development associated with a non-protective pro-inflammatory response |
spellingShingle |
Pesticide dichorvos induces early solid Ehrlich tumoral development associated with a non-protective pro-inflammatory response de Camargo, Marcela Rodrigues [UNESP] Cytokines Dichorvos Ehrlich tumor Immunotoxicity Macrophage activity Organophosphorus |
title_short |
Pesticide dichorvos induces early solid Ehrlich tumoral development associated with a non-protective pro-inflammatory response |
title_full |
Pesticide dichorvos induces early solid Ehrlich tumoral development associated with a non-protective pro-inflammatory response |
title_fullStr |
Pesticide dichorvos induces early solid Ehrlich tumoral development associated with a non-protective pro-inflammatory response |
title_full_unstemmed |
Pesticide dichorvos induces early solid Ehrlich tumoral development associated with a non-protective pro-inflammatory response |
title_sort |
Pesticide dichorvos induces early solid Ehrlich tumoral development associated with a non-protective pro-inflammatory response |
author |
de Camargo, Marcela Rodrigues [UNESP] |
author_facet |
de Camargo, Marcela Rodrigues [UNESP] Akamine, Pedro Vinicius Teruya [UNESP] Venturini, James [UNESP] de Arruda, Maria Sueli P. [UNESP] da Silva Franchi, Carla Adriene [UNESP] |
author_role |
author |
author2 |
Akamine, Pedro Vinicius Teruya [UNESP] Venturini, James [UNESP] de Arruda, Maria Sueli P. [UNESP] da Silva Franchi, Carla Adriene [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
de Camargo, Marcela Rodrigues [UNESP] Akamine, Pedro Vinicius Teruya [UNESP] Venturini, James [UNESP] de Arruda, Maria Sueli P. [UNESP] da Silva Franchi, Carla Adriene [UNESP] |
dc.subject.por.fl_str_mv |
Cytokines Dichorvos Ehrlich tumor Immunotoxicity Macrophage activity Organophosphorus |
topic |
Cytokines Dichorvos Ehrlich tumor Immunotoxicity Macrophage activity Organophosphorus |
description |
Prolonged exposure to dichlorvos (DDVP), a common pesticide used for food crops, has been related to the development of infections and malignancies. Macrophages are used as bioindicators of immunotoxicity; thus, evaluation of their activity in solid Ehrlich tumor-bearing mice (TBM) may be useful to evaluate the influence of pesticides on human health. To investigate the effects of low DDVP doses, Swiss mice were divided into the following groups: the DDVP group, composed of mice fed diets containing 10 mg/kg of DDVP; the TBM group, consisting of mice subcutaneously inoculated with 107 tumor cells/100 μl and fed a basal diet; the DDVP-TBM group, consisting of mice previously fed DDVP-containing diets for 28 days and then subcutaneously inoculated with tumor cells; and the control (CTRL) group, composed of mice fed a basal diet. After 7 and 21 days of tumor inoculation, the mice were euthanized; and after necroscopic examination, the neoplastic mass, organs, and intraperitoneal fluid were collected. Adherent peritoneal cells were cultivated to determine the production of H2O2 and TNF. Altogether, our results indicate that even at low doses, the intake of DDVP caused weight loss and increased tumor mass, which were associated with H2O2 production and high levels of TNF, a pro-inflammatory cytokine. These data are important as the exposure to pesticides, even at low doses, could potentially hinder the immune response against tumors and, consequently, create favorable conditions for their development. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T17:17:07Z 2018-12-11T17:17:07Z 2018-03-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s11356-017-1104-x Environmental Science and Pollution Research, v. 25, n. 8, p. 7681-7687, 2018. 1614-7499 0944-1344 http://hdl.handle.net/11449/175697 10.1007/s11356-017-1104-x 2-s2.0-85039552985 2-s2.0-85039552985.pdf |
url |
http://dx.doi.org/10.1007/s11356-017-1104-x http://hdl.handle.net/11449/175697 |
identifier_str_mv |
Environmental Science and Pollution Research, v. 25, n. 8, p. 7681-7687, 2018. 1614-7499 0944-1344 10.1007/s11356-017-1104-x 2-s2.0-85039552985 2-s2.0-85039552985.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Environmental Science and Pollution Research 0,858 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
7681-7687 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
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1810021395299565568 |