Pesticide dichorvos induces early solid Ehrlich tumoral development associated with a non-protective pro-inflammatory response

Detalhes bibliográficos
Autor(a) principal: de Camargo, Marcela Rodrigues [UNESP]
Data de Publicação: 2018
Outros Autores: Akamine, Pedro Vinicius Teruya [UNESP], Venturini, James [UNESP], de Arruda, Maria Sueli P. [UNESP], da Silva Franchi, Carla Adriene [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/s11356-017-1104-x
http://hdl.handle.net/11449/175697
Resumo: Prolonged exposure to dichlorvos (DDVP), a common pesticide used for food crops, has been related to the development of infections and malignancies. Macrophages are used as bioindicators of immunotoxicity; thus, evaluation of their activity in solid Ehrlich tumor-bearing mice (TBM) may be useful to evaluate the influence of pesticides on human health. To investigate the effects of low DDVP doses, Swiss mice were divided into the following groups: the DDVP group, composed of mice fed diets containing 10 mg/kg of DDVP; the TBM group, consisting of mice subcutaneously inoculated with 107 tumor cells/100 μl and fed a basal diet; the DDVP-TBM group, consisting of mice previously fed DDVP-containing diets for 28 days and then subcutaneously inoculated with tumor cells; and the control (CTRL) group, composed of mice fed a basal diet. After 7 and 21 days of tumor inoculation, the mice were euthanized; and after necroscopic examination, the neoplastic mass, organs, and intraperitoneal fluid were collected. Adherent peritoneal cells were cultivated to determine the production of H2O2 and TNF. Altogether, our results indicate that even at low doses, the intake of DDVP caused weight loss and increased tumor mass, which were associated with H2O2 production and high levels of TNF, a pro-inflammatory cytokine. These data are important as the exposure to pesticides, even at low doses, could potentially hinder the immune response against tumors and, consequently, create favorable conditions for their development.
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spelling Pesticide dichorvos induces early solid Ehrlich tumoral development associated with a non-protective pro-inflammatory responseCytokinesDichorvosEhrlich tumorImmunotoxicityMacrophage activityOrganophosphorusProlonged exposure to dichlorvos (DDVP), a common pesticide used for food crops, has been related to the development of infections and malignancies. Macrophages are used as bioindicators of immunotoxicity; thus, evaluation of their activity in solid Ehrlich tumor-bearing mice (TBM) may be useful to evaluate the influence of pesticides on human health. To investigate the effects of low DDVP doses, Swiss mice were divided into the following groups: the DDVP group, composed of mice fed diets containing 10 mg/kg of DDVP; the TBM group, consisting of mice subcutaneously inoculated with 107 tumor cells/100 μl and fed a basal diet; the DDVP-TBM group, consisting of mice previously fed DDVP-containing diets for 28 days and then subcutaneously inoculated with tumor cells; and the control (CTRL) group, composed of mice fed a basal diet. After 7 and 21 days of tumor inoculation, the mice were euthanized; and after necroscopic examination, the neoplastic mass, organs, and intraperitoneal fluid were collected. Adherent peritoneal cells were cultivated to determine the production of H2O2 and TNF. Altogether, our results indicate that even at low doses, the intake of DDVP caused weight loss and increased tumor mass, which were associated with H2O2 production and high levels of TNF, a pro-inflammatory cytokine. These data are important as the exposure to pesticides, even at low doses, could potentially hinder the immune response against tumors and, consequently, create favorable conditions for their development.Experimental Immunopathology Laboratory Department of Biological Sciences College of Sciences Universidade Estadual Paulista Julio de Mesquita Filho – UNESPDepartment of Pathology Botucatu Medical School Universidade Estadual Paulista Julio de Mesquita Filho – UNESPExperimental Immunopathology Laboratory Department of Biological Sciences College of Sciences Universidade Estadual Paulista Julio de Mesquita Filho – UNESPDepartment of Pathology Botucatu Medical School Universidade Estadual Paulista Julio de Mesquita Filho – UNESPUniversidade Estadual Paulista (Unesp)de Camargo, Marcela Rodrigues [UNESP]Akamine, Pedro Vinicius Teruya [UNESP]Venturini, James [UNESP]de Arruda, Maria Sueli P. [UNESP]da Silva Franchi, Carla Adriene [UNESP]2018-12-11T17:17:07Z2018-12-11T17:17:07Z2018-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article7681-7687application/pdfhttp://dx.doi.org/10.1007/s11356-017-1104-xEnvironmental Science and Pollution Research, v. 25, n. 8, p. 7681-7687, 2018.1614-74990944-1344http://hdl.handle.net/11449/17569710.1007/s11356-017-1104-x2-s2.0-850395529852-s2.0-85039552985.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEnvironmental Science and Pollution Research0,858info:eu-repo/semantics/openAccess2024-09-03T13:15:29Zoai:repositorio.unesp.br:11449/175697Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-03T13:15:29Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Pesticide dichorvos induces early solid Ehrlich tumoral development associated with a non-protective pro-inflammatory response
title Pesticide dichorvos induces early solid Ehrlich tumoral development associated with a non-protective pro-inflammatory response
spellingShingle Pesticide dichorvos induces early solid Ehrlich tumoral development associated with a non-protective pro-inflammatory response
de Camargo, Marcela Rodrigues [UNESP]
Cytokines
Dichorvos
Ehrlich tumor
Immunotoxicity
Macrophage activity
Organophosphorus
title_short Pesticide dichorvos induces early solid Ehrlich tumoral development associated with a non-protective pro-inflammatory response
title_full Pesticide dichorvos induces early solid Ehrlich tumoral development associated with a non-protective pro-inflammatory response
title_fullStr Pesticide dichorvos induces early solid Ehrlich tumoral development associated with a non-protective pro-inflammatory response
title_full_unstemmed Pesticide dichorvos induces early solid Ehrlich tumoral development associated with a non-protective pro-inflammatory response
title_sort Pesticide dichorvos induces early solid Ehrlich tumoral development associated with a non-protective pro-inflammatory response
author de Camargo, Marcela Rodrigues [UNESP]
author_facet de Camargo, Marcela Rodrigues [UNESP]
Akamine, Pedro Vinicius Teruya [UNESP]
Venturini, James [UNESP]
de Arruda, Maria Sueli P. [UNESP]
da Silva Franchi, Carla Adriene [UNESP]
author_role author
author2 Akamine, Pedro Vinicius Teruya [UNESP]
Venturini, James [UNESP]
de Arruda, Maria Sueli P. [UNESP]
da Silva Franchi, Carla Adriene [UNESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv de Camargo, Marcela Rodrigues [UNESP]
Akamine, Pedro Vinicius Teruya [UNESP]
Venturini, James [UNESP]
de Arruda, Maria Sueli P. [UNESP]
da Silva Franchi, Carla Adriene [UNESP]
dc.subject.por.fl_str_mv Cytokines
Dichorvos
Ehrlich tumor
Immunotoxicity
Macrophage activity
Organophosphorus
topic Cytokines
Dichorvos
Ehrlich tumor
Immunotoxicity
Macrophage activity
Organophosphorus
description Prolonged exposure to dichlorvos (DDVP), a common pesticide used for food crops, has been related to the development of infections and malignancies. Macrophages are used as bioindicators of immunotoxicity; thus, evaluation of their activity in solid Ehrlich tumor-bearing mice (TBM) may be useful to evaluate the influence of pesticides on human health. To investigate the effects of low DDVP doses, Swiss mice were divided into the following groups: the DDVP group, composed of mice fed diets containing 10 mg/kg of DDVP; the TBM group, consisting of mice subcutaneously inoculated with 107 tumor cells/100 μl and fed a basal diet; the DDVP-TBM group, consisting of mice previously fed DDVP-containing diets for 28 days and then subcutaneously inoculated with tumor cells; and the control (CTRL) group, composed of mice fed a basal diet. After 7 and 21 days of tumor inoculation, the mice were euthanized; and after necroscopic examination, the neoplastic mass, organs, and intraperitoneal fluid were collected. Adherent peritoneal cells were cultivated to determine the production of H2O2 and TNF. Altogether, our results indicate that even at low doses, the intake of DDVP caused weight loss and increased tumor mass, which were associated with H2O2 production and high levels of TNF, a pro-inflammatory cytokine. These data are important as the exposure to pesticides, even at low doses, could potentially hinder the immune response against tumors and, consequently, create favorable conditions for their development.
publishDate 2018
dc.date.none.fl_str_mv 2018-12-11T17:17:07Z
2018-12-11T17:17:07Z
2018-03-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s11356-017-1104-x
Environmental Science and Pollution Research, v. 25, n. 8, p. 7681-7687, 2018.
1614-7499
0944-1344
http://hdl.handle.net/11449/175697
10.1007/s11356-017-1104-x
2-s2.0-85039552985
2-s2.0-85039552985.pdf
url http://dx.doi.org/10.1007/s11356-017-1104-x
http://hdl.handle.net/11449/175697
identifier_str_mv Environmental Science and Pollution Research, v. 25, n. 8, p. 7681-7687, 2018.
1614-7499
0944-1344
10.1007/s11356-017-1104-x
2-s2.0-85039552985
2-s2.0-85039552985.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Environmental Science and Pollution Research
0,858
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 7681-7687
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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