Development of an oral mucosa equivalent using a porcine dermal matrix
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.bjoms.2016.09.019 http://hdl.handle.net/11449/173665 |
Resumo: | We evaluated the suitability of a porcine acellular dermal matrix for the development of a 3-dimensional oral mucosal equivalent using an ex vivo-produced oral mucosal equivalent (EVPOME). Oral keratinocytes were seeded in a submerged model, and then in an air-liquid interphase model, using Transwell® inserts. EVPOME showed good cell viability and increased glucose consumption over time. Histological evaluation showed that stratified differentiated epithelium had formed in all matrices. |
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Repositório Institucional da UNESP |
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2946 |
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Development of an oral mucosa equivalent using a porcine dermal matrixOral keratinocytesoral mucosaorganotypic cell cultureWe evaluated the suitability of a porcine acellular dermal matrix for the development of a 3-dimensional oral mucosal equivalent using an ex vivo-produced oral mucosal equivalent (EVPOME). Oral keratinocytes were seeded in a submerged model, and then in an air-liquid interphase model, using Transwell® inserts. EVPOME showed good cell viability and increased glucose consumption over time. Histological evaluation showed that stratified differentiated epithelium had formed in all matrices.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Physiology and Pathology Araraquara Dental School UNESPDepartment of Pediatric Dentistry Araraquara Dental School UNESPDepartment of Oral and Maxillofacial Surgery University of Michigan Health SystemDepartment of Physiology and Pathology Araraquara Dental School UNESPDepartment of Pediatric Dentistry Araraquara Dental School UNESPFAPESP: 2013/05879-0FAPESP: BEPE/PD: 2014/06057-7FAPESP: PD: 2012/17947-8Universidade Estadual Paulista (Unesp)University of Michigan Health SystemBasso, F. G. [UNESP]Hebling, J. [UNESP]Marcelo, C. L.de Souza Costa, C. A. [UNESP]Feinberg, S. E.2018-12-11T17:07:09Z2018-12-11T17:07:09Z2017-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article308-311application/pdfhttp://dx.doi.org/10.1016/j.bjoms.2016.09.019British Journal of Oral and Maxillofacial Surgery, v. 55, n. 3, p. 308-311, 2017.1532-19400266-4356http://hdl.handle.net/11449/17366510.1016/j.bjoms.2016.09.0192-s2.0-849921688432-s2.0-84992168843.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBritish Journal of Oral and Maxillofacial Surgery0,687info:eu-repo/semantics/openAccess2024-09-27T14:05:25Zoai:repositorio.unesp.br:11449/173665Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-27T14:05:25Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Development of an oral mucosa equivalent using a porcine dermal matrix |
title |
Development of an oral mucosa equivalent using a porcine dermal matrix |
spellingShingle |
Development of an oral mucosa equivalent using a porcine dermal matrix Basso, F. G. [UNESP] Oral keratinocytes oral mucosa organotypic cell culture |
title_short |
Development of an oral mucosa equivalent using a porcine dermal matrix |
title_full |
Development of an oral mucosa equivalent using a porcine dermal matrix |
title_fullStr |
Development of an oral mucosa equivalent using a porcine dermal matrix |
title_full_unstemmed |
Development of an oral mucosa equivalent using a porcine dermal matrix |
title_sort |
Development of an oral mucosa equivalent using a porcine dermal matrix |
author |
Basso, F. G. [UNESP] |
author_facet |
Basso, F. G. [UNESP] Hebling, J. [UNESP] Marcelo, C. L. de Souza Costa, C. A. [UNESP] Feinberg, S. E. |
author_role |
author |
author2 |
Hebling, J. [UNESP] Marcelo, C. L. de Souza Costa, C. A. [UNESP] Feinberg, S. E. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) University of Michigan Health System |
dc.contributor.author.fl_str_mv |
Basso, F. G. [UNESP] Hebling, J. [UNESP] Marcelo, C. L. de Souza Costa, C. A. [UNESP] Feinberg, S. E. |
dc.subject.por.fl_str_mv |
Oral keratinocytes oral mucosa organotypic cell culture |
topic |
Oral keratinocytes oral mucosa organotypic cell culture |
description |
We evaluated the suitability of a porcine acellular dermal matrix for the development of a 3-dimensional oral mucosal equivalent using an ex vivo-produced oral mucosal equivalent (EVPOME). Oral keratinocytes were seeded in a submerged model, and then in an air-liquid interphase model, using Transwell® inserts. EVPOME showed good cell viability and increased glucose consumption over time. Histological evaluation showed that stratified differentiated epithelium had formed in all matrices. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-04-01 2018-12-11T17:07:09Z 2018-12-11T17:07:09Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.bjoms.2016.09.019 British Journal of Oral and Maxillofacial Surgery, v. 55, n. 3, p. 308-311, 2017. 1532-1940 0266-4356 http://hdl.handle.net/11449/173665 10.1016/j.bjoms.2016.09.019 2-s2.0-84992168843 2-s2.0-84992168843.pdf |
url |
http://dx.doi.org/10.1016/j.bjoms.2016.09.019 http://hdl.handle.net/11449/173665 |
identifier_str_mv |
British Journal of Oral and Maxillofacial Surgery, v. 55, n. 3, p. 308-311, 2017. 1532-1940 0266-4356 10.1016/j.bjoms.2016.09.019 2-s2.0-84992168843 2-s2.0-84992168843.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
British Journal of Oral and Maxillofacial Surgery 0,687 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
308-311 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1813546463613943808 |