Development of an oral mucosa equivalent using a porcine dermal matrix

Detalhes bibliográficos
Autor(a) principal: Basso, F. G. [UNESP]
Data de Publicação: 2017
Outros Autores: Hebling, J. [UNESP], Marcelo, C. L., de Souza Costa, C. A. [UNESP], Feinberg, S. E.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.bjoms.2016.09.019
http://hdl.handle.net/11449/173665
Resumo: We evaluated the suitability of a porcine acellular dermal matrix for the development of a 3-dimensional oral mucosal equivalent using an ex vivo-produced oral mucosal equivalent (EVPOME). Oral keratinocytes were seeded in a submerged model, and then in an air-liquid interphase model, using Transwell® inserts. EVPOME showed good cell viability and increased glucose consumption over time. Histological evaluation showed that stratified differentiated epithelium had formed in all matrices.
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spelling Development of an oral mucosa equivalent using a porcine dermal matrixOral keratinocytesoral mucosaorganotypic cell cultureWe evaluated the suitability of a porcine acellular dermal matrix for the development of a 3-dimensional oral mucosal equivalent using an ex vivo-produced oral mucosal equivalent (EVPOME). Oral keratinocytes were seeded in a submerged model, and then in an air-liquid interphase model, using Transwell® inserts. EVPOME showed good cell viability and increased glucose consumption over time. Histological evaluation showed that stratified differentiated epithelium had formed in all matrices.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Physiology and Pathology Araraquara Dental School UNESPDepartment of Pediatric Dentistry Araraquara Dental School UNESPDepartment of Oral and Maxillofacial Surgery University of Michigan Health SystemDepartment of Physiology and Pathology Araraquara Dental School UNESPDepartment of Pediatric Dentistry Araraquara Dental School UNESPFAPESP: 2013/05879-0FAPESP: BEPE/PD: 2014/06057-7FAPESP: PD: 2012/17947-8Universidade Estadual Paulista (Unesp)University of Michigan Health SystemBasso, F. G. [UNESP]Hebling, J. [UNESP]Marcelo, C. L.de Souza Costa, C. A. [UNESP]Feinberg, S. E.2018-12-11T17:07:09Z2018-12-11T17:07:09Z2017-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article308-311application/pdfhttp://dx.doi.org/10.1016/j.bjoms.2016.09.019British Journal of Oral and Maxillofacial Surgery, v. 55, n. 3, p. 308-311, 2017.1532-19400266-4356http://hdl.handle.net/11449/17366510.1016/j.bjoms.2016.09.0192-s2.0-849921688432-s2.0-84992168843.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBritish Journal of Oral and Maxillofacial Surgery0,687info:eu-repo/semantics/openAccess2024-09-27T14:05:25Zoai:repositorio.unesp.br:11449/173665Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-27T14:05:25Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Development of an oral mucosa equivalent using a porcine dermal matrix
title Development of an oral mucosa equivalent using a porcine dermal matrix
spellingShingle Development of an oral mucosa equivalent using a porcine dermal matrix
Basso, F. G. [UNESP]
Oral keratinocytes
oral mucosa
organotypic cell culture
title_short Development of an oral mucosa equivalent using a porcine dermal matrix
title_full Development of an oral mucosa equivalent using a porcine dermal matrix
title_fullStr Development of an oral mucosa equivalent using a porcine dermal matrix
title_full_unstemmed Development of an oral mucosa equivalent using a porcine dermal matrix
title_sort Development of an oral mucosa equivalent using a porcine dermal matrix
author Basso, F. G. [UNESP]
author_facet Basso, F. G. [UNESP]
Hebling, J. [UNESP]
Marcelo, C. L.
de Souza Costa, C. A. [UNESP]
Feinberg, S. E.
author_role author
author2 Hebling, J. [UNESP]
Marcelo, C. L.
de Souza Costa, C. A. [UNESP]
Feinberg, S. E.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
University of Michigan Health System
dc.contributor.author.fl_str_mv Basso, F. G. [UNESP]
Hebling, J. [UNESP]
Marcelo, C. L.
de Souza Costa, C. A. [UNESP]
Feinberg, S. E.
dc.subject.por.fl_str_mv Oral keratinocytes
oral mucosa
organotypic cell culture
topic Oral keratinocytes
oral mucosa
organotypic cell culture
description We evaluated the suitability of a porcine acellular dermal matrix for the development of a 3-dimensional oral mucosal equivalent using an ex vivo-produced oral mucosal equivalent (EVPOME). Oral keratinocytes were seeded in a submerged model, and then in an air-liquid interphase model, using Transwell® inserts. EVPOME showed good cell viability and increased glucose consumption over time. Histological evaluation showed that stratified differentiated epithelium had formed in all matrices.
publishDate 2017
dc.date.none.fl_str_mv 2017-04-01
2018-12-11T17:07:09Z
2018-12-11T17:07:09Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.bjoms.2016.09.019
British Journal of Oral and Maxillofacial Surgery, v. 55, n. 3, p. 308-311, 2017.
1532-1940
0266-4356
http://hdl.handle.net/11449/173665
10.1016/j.bjoms.2016.09.019
2-s2.0-84992168843
2-s2.0-84992168843.pdf
url http://dx.doi.org/10.1016/j.bjoms.2016.09.019
http://hdl.handle.net/11449/173665
identifier_str_mv British Journal of Oral and Maxillofacial Surgery, v. 55, n. 3, p. 308-311, 2017.
1532-1940
0266-4356
10.1016/j.bjoms.2016.09.019
2-s2.0-84992168843
2-s2.0-84992168843.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv British Journal of Oral and Maxillofacial Surgery
0,687
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 308-311
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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