First Report of a New Isolate of Metarhizium rileyi from Maize Fields of Quivicán, Cuba
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s12088-018-0721-5 http://hdl.handle.net/11449/176057 |
Resumo: | Metarhizium rileyi (Farlow) Samson is an important entomopathogenic fungus of more than 30 species of Lepidoptera larvae. The aim of this research was to characterize isolate of M. rileyi from Quivicán, Cuba on the basis of morphological and molecular approaches. The fungus was isolated from samples of S. frugiperda larvae collected from maize fields of Quivicán municipality, Mayabeque province, Cuba, and it was cultured on PDA + Ampicillin solid media for morphological characterization. The DNA was isolated using CTAB method and internal transcribed spacer (ITS1, ITS4) were used as the primers for the amplification. The amplified products of 1335 bp were purified and sequenced at CINVESTAV-IPN in both the directions using the above primers. A consensus sequence was obtained by alignment of the forward and reverse sequences for this region and deposited in GenBank (MG637450). The fungus produced slightly cottony colony of pale green color and dispersed conidia and septal mycelium were observed under the optical microscope. A BLAST search of the sequence in GenBank revealed a 99% of identity with several strains of N. rileyi (e.g., AF368501.1, AB268359.1 and EU553337.1) and M. rileyi (e.g., KY436756.1). This is the first report of M. rileyi isolate from maize fields of Quivicán in Cuba and this is important for biodiversity studies and is another possibility for Integrated Pest Management. |
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First Report of a New Isolate of Metarhizium rileyi from Maize Fields of Quivicán, CubaBiological controlEntomopathogen fungiLepidopteraMetarhizium rileyiMetarhizium rileyi (Farlow) Samson is an important entomopathogenic fungus of more than 30 species of Lepidoptera larvae. The aim of this research was to characterize isolate of M. rileyi from Quivicán, Cuba on the basis of morphological and molecular approaches. The fungus was isolated from samples of S. frugiperda larvae collected from maize fields of Quivicán municipality, Mayabeque province, Cuba, and it was cultured on PDA + Ampicillin solid media for morphological characterization. The DNA was isolated using CTAB method and internal transcribed spacer (ITS1, ITS4) were used as the primers for the amplification. The amplified products of 1335 bp were purified and sequenced at CINVESTAV-IPN in both the directions using the above primers. A consensus sequence was obtained by alignment of the forward and reverse sequences for this region and deposited in GenBank (MG637450). The fungus produced slightly cottony colony of pale green color and dispersed conidia and septal mycelium were observed under the optical microscope. A BLAST search of the sequence in GenBank revealed a 99% of identity with several strains of N. rileyi (e.g., AF368501.1, AB268359.1 and EU553337.1) and M. rileyi (e.g., KY436756.1). This is the first report of M. rileyi isolate from maize fields of Quivicán in Cuba and this is important for biodiversity studies and is another possibility for Integrated Pest Management.Depto. de Biotecnología Agrícola Instituto Politécnico Nacional CIIDIR Unidad Sinaloa, Blvd. Juan de Dios Bátiz Paredes 250Empresa Bioagricultura Aplicada AMG Calle Santos Degollado entre Guillermo Nelson y Cuauhtemoc, Colonia CentroInstituto de Biociências Universidade Estadual Paulista (UNESP), Câmpus São Vicente, Praça Infante Dom Henrique s/n, Parque BitaruInstituto de Biociências Universidade Estadual Paulista (UNESP), Câmpus São Vicente, Praça Infante Dom Henrique s/n, Parque BitaruCIIDIR Unidad SinaloaCalle Santos Degollado entre Guillermo Nelson y CuauhtemocUniversidade Estadual Paulista (Unesp)Álvarez, Sandra PérezGuerrero, Amaury MéndezDuarte, Bernardo Nayar DéboraTapia, Marco Antonio MagallanesMedina, Jesús Alicia ChávezDomínguez Rodríguez, Yoannis [UNESP]2018-12-11T17:18:44Z2018-12-11T17:18:44Z2018-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article222-226application/pdfhttp://dx.doi.org/10.1007/s12088-018-0721-5Indian Journal of Microbiology, v. 58, n. 2, p. 222-226, 2018.0046-8991http://hdl.handle.net/11449/17605710.1007/s12088-018-0721-52-s2.0-850443256082-s2.0-85044325608.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengIndian Journal of Microbiology0,473info:eu-repo/semantics/openAccess2023-11-01T06:10:51Zoai:repositorio.unesp.br:11449/176057Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:36:42.772607Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
First Report of a New Isolate of Metarhizium rileyi from Maize Fields of Quivicán, Cuba |
title |
First Report of a New Isolate of Metarhizium rileyi from Maize Fields of Quivicán, Cuba |
spellingShingle |
First Report of a New Isolate of Metarhizium rileyi from Maize Fields of Quivicán, Cuba Álvarez, Sandra Pérez Biological control Entomopathogen fungi Lepidoptera Metarhizium rileyi |
title_short |
First Report of a New Isolate of Metarhizium rileyi from Maize Fields of Quivicán, Cuba |
title_full |
First Report of a New Isolate of Metarhizium rileyi from Maize Fields of Quivicán, Cuba |
title_fullStr |
First Report of a New Isolate of Metarhizium rileyi from Maize Fields of Quivicán, Cuba |
title_full_unstemmed |
First Report of a New Isolate of Metarhizium rileyi from Maize Fields of Quivicán, Cuba |
title_sort |
First Report of a New Isolate of Metarhizium rileyi from Maize Fields of Quivicán, Cuba |
author |
Álvarez, Sandra Pérez |
author_facet |
Álvarez, Sandra Pérez Guerrero, Amaury Méndez Duarte, Bernardo Nayar Débora Tapia, Marco Antonio Magallanes Medina, Jesús Alicia Chávez Domínguez Rodríguez, Yoannis [UNESP] |
author_role |
author |
author2 |
Guerrero, Amaury Méndez Duarte, Bernardo Nayar Débora Tapia, Marco Antonio Magallanes Medina, Jesús Alicia Chávez Domínguez Rodríguez, Yoannis [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
CIIDIR Unidad Sinaloa Calle Santos Degollado entre Guillermo Nelson y Cuauhtemoc Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Álvarez, Sandra Pérez Guerrero, Amaury Méndez Duarte, Bernardo Nayar Débora Tapia, Marco Antonio Magallanes Medina, Jesús Alicia Chávez Domínguez Rodríguez, Yoannis [UNESP] |
dc.subject.por.fl_str_mv |
Biological control Entomopathogen fungi Lepidoptera Metarhizium rileyi |
topic |
Biological control Entomopathogen fungi Lepidoptera Metarhizium rileyi |
description |
Metarhizium rileyi (Farlow) Samson is an important entomopathogenic fungus of more than 30 species of Lepidoptera larvae. The aim of this research was to characterize isolate of M. rileyi from Quivicán, Cuba on the basis of morphological and molecular approaches. The fungus was isolated from samples of S. frugiperda larvae collected from maize fields of Quivicán municipality, Mayabeque province, Cuba, and it was cultured on PDA + Ampicillin solid media for morphological characterization. The DNA was isolated using CTAB method and internal transcribed spacer (ITS1, ITS4) were used as the primers for the amplification. The amplified products of 1335 bp were purified and sequenced at CINVESTAV-IPN in both the directions using the above primers. A consensus sequence was obtained by alignment of the forward and reverse sequences for this region and deposited in GenBank (MG637450). The fungus produced slightly cottony colony of pale green color and dispersed conidia and septal mycelium were observed under the optical microscope. A BLAST search of the sequence in GenBank revealed a 99% of identity with several strains of N. rileyi (e.g., AF368501.1, AB268359.1 and EU553337.1) and M. rileyi (e.g., KY436756.1). This is the first report of M. rileyi isolate from maize fields of Quivicán in Cuba and this is important for biodiversity studies and is another possibility for Integrated Pest Management. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T17:18:44Z 2018-12-11T17:18:44Z 2018-06-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s12088-018-0721-5 Indian Journal of Microbiology, v. 58, n. 2, p. 222-226, 2018. 0046-8991 http://hdl.handle.net/11449/176057 10.1007/s12088-018-0721-5 2-s2.0-85044325608 2-s2.0-85044325608.pdf |
url |
http://dx.doi.org/10.1007/s12088-018-0721-5 http://hdl.handle.net/11449/176057 |
identifier_str_mv |
Indian Journal of Microbiology, v. 58, n. 2, p. 222-226, 2018. 0046-8991 10.1007/s12088-018-0721-5 2-s2.0-85044325608 2-s2.0-85044325608.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Indian Journal of Microbiology 0,473 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
222-226 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128678482149376 |