First Report of a New Isolate of Metarhizium rileyi from Maize Fields of Quivicán, Cuba

Detalhes bibliográficos
Autor(a) principal: Álvarez, Sandra Pérez
Data de Publicação: 2018
Outros Autores: Guerrero, Amaury Méndez, Duarte, Bernardo Nayar Débora, Tapia, Marco Antonio Magallanes, Medina, Jesús Alicia Chávez, Domínguez Rodríguez, Yoannis [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/s12088-018-0721-5
http://hdl.handle.net/11449/176057
Resumo: Metarhizium rileyi (Farlow) Samson is an important entomopathogenic fungus of more than 30 species of Lepidoptera larvae. The aim of this research was to characterize isolate of M. rileyi from Quivicán, Cuba on the basis of morphological and molecular approaches. The fungus was isolated from samples of S. frugiperda larvae collected from maize fields of Quivicán municipality, Mayabeque province, Cuba, and it was cultured on PDA + Ampicillin solid media for morphological characterization. The DNA was isolated using CTAB method and internal transcribed spacer (ITS1, ITS4) were used as the primers for the amplification. The amplified products of 1335 bp were purified and sequenced at CINVESTAV-IPN in both the directions using the above primers. A consensus sequence was obtained by alignment of the forward and reverse sequences for this region and deposited in GenBank (MG637450). The fungus produced slightly cottony colony of pale green color and dispersed conidia and septal mycelium were observed under the optical microscope. A BLAST search of the sequence in GenBank revealed a 99% of identity with several strains of N. rileyi (e.g., AF368501.1, AB268359.1 and EU553337.1) and M. rileyi (e.g., KY436756.1). This is the first report of M. rileyi isolate from maize fields of Quivicán in Cuba and this is important for biodiversity studies and is another possibility for Integrated Pest Management.
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spelling First Report of a New Isolate of Metarhizium rileyi from Maize Fields of Quivicán, CubaBiological controlEntomopathogen fungiLepidopteraMetarhizium rileyiMetarhizium rileyi (Farlow) Samson is an important entomopathogenic fungus of more than 30 species of Lepidoptera larvae. The aim of this research was to characterize isolate of M. rileyi from Quivicán, Cuba on the basis of morphological and molecular approaches. The fungus was isolated from samples of S. frugiperda larvae collected from maize fields of Quivicán municipality, Mayabeque province, Cuba, and it was cultured on PDA + Ampicillin solid media for morphological characterization. The DNA was isolated using CTAB method and internal transcribed spacer (ITS1, ITS4) were used as the primers for the amplification. The amplified products of 1335 bp were purified and sequenced at CINVESTAV-IPN in both the directions using the above primers. A consensus sequence was obtained by alignment of the forward and reverse sequences for this region and deposited in GenBank (MG637450). The fungus produced slightly cottony colony of pale green color and dispersed conidia and septal mycelium were observed under the optical microscope. A BLAST search of the sequence in GenBank revealed a 99% of identity with several strains of N. rileyi (e.g., AF368501.1, AB268359.1 and EU553337.1) and M. rileyi (e.g., KY436756.1). This is the first report of M. rileyi isolate from maize fields of Quivicán in Cuba and this is important for biodiversity studies and is another possibility for Integrated Pest Management.Depto. de Biotecnología Agrícola Instituto Politécnico Nacional CIIDIR Unidad Sinaloa, Blvd. Juan de Dios Bátiz Paredes 250Empresa Bioagricultura Aplicada AMG Calle Santos Degollado entre Guillermo Nelson y Cuauhtemoc, Colonia CentroInstituto de Biociências Universidade Estadual Paulista (UNESP), Câmpus São Vicente, Praça Infante Dom Henrique s/n, Parque BitaruInstituto de Biociências Universidade Estadual Paulista (UNESP), Câmpus São Vicente, Praça Infante Dom Henrique s/n, Parque BitaruCIIDIR Unidad SinaloaCalle Santos Degollado entre Guillermo Nelson y CuauhtemocUniversidade Estadual Paulista (Unesp)Álvarez, Sandra PérezGuerrero, Amaury MéndezDuarte, Bernardo Nayar DéboraTapia, Marco Antonio MagallanesMedina, Jesús Alicia ChávezDomínguez Rodríguez, Yoannis [UNESP]2018-12-11T17:18:44Z2018-12-11T17:18:44Z2018-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article222-226application/pdfhttp://dx.doi.org/10.1007/s12088-018-0721-5Indian Journal of Microbiology, v. 58, n. 2, p. 222-226, 2018.0046-8991http://hdl.handle.net/11449/17605710.1007/s12088-018-0721-52-s2.0-850443256082-s2.0-85044325608.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengIndian Journal of Microbiology0,473info:eu-repo/semantics/openAccess2023-11-01T06:10:51Zoai:repositorio.unesp.br:11449/176057Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:36:42.772607Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv First Report of a New Isolate of Metarhizium rileyi from Maize Fields of Quivicán, Cuba
title First Report of a New Isolate of Metarhizium rileyi from Maize Fields of Quivicán, Cuba
spellingShingle First Report of a New Isolate of Metarhizium rileyi from Maize Fields of Quivicán, Cuba
Álvarez, Sandra Pérez
Biological control
Entomopathogen fungi
Lepidoptera
Metarhizium rileyi
title_short First Report of a New Isolate of Metarhizium rileyi from Maize Fields of Quivicán, Cuba
title_full First Report of a New Isolate of Metarhizium rileyi from Maize Fields of Quivicán, Cuba
title_fullStr First Report of a New Isolate of Metarhizium rileyi from Maize Fields of Quivicán, Cuba
title_full_unstemmed First Report of a New Isolate of Metarhizium rileyi from Maize Fields of Quivicán, Cuba
title_sort First Report of a New Isolate of Metarhizium rileyi from Maize Fields of Quivicán, Cuba
author Álvarez, Sandra Pérez
author_facet Álvarez, Sandra Pérez
Guerrero, Amaury Méndez
Duarte, Bernardo Nayar Débora
Tapia, Marco Antonio Magallanes
Medina, Jesús Alicia Chávez
Domínguez Rodríguez, Yoannis [UNESP]
author_role author
author2 Guerrero, Amaury Méndez
Duarte, Bernardo Nayar Débora
Tapia, Marco Antonio Magallanes
Medina, Jesús Alicia Chávez
Domínguez Rodríguez, Yoannis [UNESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv CIIDIR Unidad Sinaloa
Calle Santos Degollado entre Guillermo Nelson y Cuauhtemoc
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Álvarez, Sandra Pérez
Guerrero, Amaury Méndez
Duarte, Bernardo Nayar Débora
Tapia, Marco Antonio Magallanes
Medina, Jesús Alicia Chávez
Domínguez Rodríguez, Yoannis [UNESP]
dc.subject.por.fl_str_mv Biological control
Entomopathogen fungi
Lepidoptera
Metarhizium rileyi
topic Biological control
Entomopathogen fungi
Lepidoptera
Metarhizium rileyi
description Metarhizium rileyi (Farlow) Samson is an important entomopathogenic fungus of more than 30 species of Lepidoptera larvae. The aim of this research was to characterize isolate of M. rileyi from Quivicán, Cuba on the basis of morphological and molecular approaches. The fungus was isolated from samples of S. frugiperda larvae collected from maize fields of Quivicán municipality, Mayabeque province, Cuba, and it was cultured on PDA + Ampicillin solid media for morphological characterization. The DNA was isolated using CTAB method and internal transcribed spacer (ITS1, ITS4) were used as the primers for the amplification. The amplified products of 1335 bp were purified and sequenced at CINVESTAV-IPN in both the directions using the above primers. A consensus sequence was obtained by alignment of the forward and reverse sequences for this region and deposited in GenBank (MG637450). The fungus produced slightly cottony colony of pale green color and dispersed conidia and septal mycelium were observed under the optical microscope. A BLAST search of the sequence in GenBank revealed a 99% of identity with several strains of N. rileyi (e.g., AF368501.1, AB268359.1 and EU553337.1) and M. rileyi (e.g., KY436756.1). This is the first report of M. rileyi isolate from maize fields of Quivicán in Cuba and this is important for biodiversity studies and is another possibility for Integrated Pest Management.
publishDate 2018
dc.date.none.fl_str_mv 2018-12-11T17:18:44Z
2018-12-11T17:18:44Z
2018-06-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s12088-018-0721-5
Indian Journal of Microbiology, v. 58, n. 2, p. 222-226, 2018.
0046-8991
http://hdl.handle.net/11449/176057
10.1007/s12088-018-0721-5
2-s2.0-85044325608
2-s2.0-85044325608.pdf
url http://dx.doi.org/10.1007/s12088-018-0721-5
http://hdl.handle.net/11449/176057
identifier_str_mv Indian Journal of Microbiology, v. 58, n. 2, p. 222-226, 2018.
0046-8991
10.1007/s12088-018-0721-5
2-s2.0-85044325608
2-s2.0-85044325608.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Indian Journal of Microbiology
0,473
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 222-226
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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