Optimization and validation of analytical methodology for determination of 1-hydroxypyrene in urine of sugar cane workers
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.26850/1678-4618EQJ.V35.4.2010.P113-119 http://hdl.handle.net/11449/229681 |
Resumo: | This study aimed to optimize and validate an analytical methodology for determination of 1- hydroxypyrene in urine of workers involved in harvesting sugar cane. The method used for determining 1- hydroxypyrene in human urine is the enzymatic hydrolysis, extraction and clean-up by solid phase extraction (SPE) and quantified by high performance liquid chromatography with fluorescence detection (HPLC / Flu). Four types of cartridges were tested to verify the percentage of recovery. Urine of sugar-cane workers (nonsmokers and of both sexes) were collected during the harvest (n = 39) and non-harvest season (n = 34) of cane sugar. The best recovery results were attributed to the C18 cartridges. They presented recovery between 79% and 108%, with a coefficient of variation between 5% and 10%. The limit of quantification was 74 ng of 1- hydroxypyrene per liter of urine. The optimized and validated methodology was used for determination of real samples. The results found in the urine of workers at harvest period ranged from 0.026 to 2.3 mol of 1- hydroxypyrene per mol creatinine. During the non-harvesting season the results ranged from 0.0023 to 0.38 mol of 1-hydroxypyrene per mol creatinine. The validated methodology proved suitable for determination of 1-hydroxypyrene in human urine. The data indicate that there is strong correlation between excretion of 1- hydroxypyrene and the periods during and between harvests of sugar cane. |
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Optimization and validation of analytical methodology for determination of 1-hydroxypyrene in urine of sugar cane workersOtimizaÇÃo e validaÇÃo de uma metodologia analítica para determinaÇÃo de 1- hidroxipireno em urina de cortadores de cana-de-aÇÚcar1-hydroxypyreneOccupational healthSugar-cane workersUrineValidation of analytical methodologyThis study aimed to optimize and validate an analytical methodology for determination of 1- hydroxypyrene in urine of workers involved in harvesting sugar cane. The method used for determining 1- hydroxypyrene in human urine is the enzymatic hydrolysis, extraction and clean-up by solid phase extraction (SPE) and quantified by high performance liquid chromatography with fluorescence detection (HPLC / Flu). Four types of cartridges were tested to verify the percentage of recovery. Urine of sugar-cane workers (nonsmokers and of both sexes) were collected during the harvest (n = 39) and non-harvest season (n = 34) of cane sugar. The best recovery results were attributed to the C18 cartridges. They presented recovery between 79% and 108%, with a coefficient of variation between 5% and 10%. The limit of quantification was 74 ng of 1- hydroxypyrene per liter of urine. The optimized and validated methodology was used for determination of real samples. The results found in the urine of workers at harvest period ranged from 0.026 to 2.3 mol of 1- hydroxypyrene per mol creatinine. During the non-harvesting season the results ranged from 0.0023 to 0.38 mol of 1-hydroxypyrene per mol creatinine. The validated methodology proved suitable for determination of 1-hydroxypyrene in human urine. The data indicate that there is strong correlation between excretion of 1- hydroxypyrene and the periods during and between harvests of sugar cane.Departamento de Química Analítica Instituto de Química Universidade Estadual Paulista (UNESP), Caixa Postal 355 (SP)Departamento de Química Orgânica Instituto de Química Universidade Estadual Paulista (UNESP), Caixa Postal 355 (SP)Departamento de Química Analítica Instituto de Química Universidade Estadual Paulista (UNESP), Caixa Postal 355 (SP)Departamento de Química Orgânica Instituto de Química Universidade Estadual Paulista (UNESP), Caixa Postal 355 (SP)Universidade Estadual Paulista (UNESP)Sakiara, K. A. [UNESP]De Andrade, S. J. [UNESP]Marchi, M. R.R. [UNESP]Vilegas, W. [UNESP]Bosso, R. M.V.Conforti-Froes, N. D.T.2022-04-29T08:35:05Z2022-04-29T08:35:05Z2010-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article113-119http://dx.doi.org/10.26850/1678-4618EQJ.V35.4.2010.P113-119Ecletica Quimica, v. 35, n. 4, p. 113-119, 2010.1678-46180100-4670http://hdl.handle.net/11449/22968110.26850/1678-4618EQJ.V35.4.2010.P113-1192-s2.0-85116830704Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporEcletica Quimicainfo:eu-repo/semantics/openAccess2022-04-29T08:35:05Zoai:repositorio.unesp.br:11449/229681Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:45:11.562917Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Optimization and validation of analytical methodology for determination of 1-hydroxypyrene in urine of sugar cane workers OtimizaÇÃo e validaÇÃo de uma metodologia analítica para determinaÇÃo de 1- hidroxipireno em urina de cortadores de cana-de-aÇÚcar |
title |
Optimization and validation of analytical methodology for determination of 1-hydroxypyrene in urine of sugar cane workers |
spellingShingle |
Optimization and validation of analytical methodology for determination of 1-hydroxypyrene in urine of sugar cane workers Sakiara, K. A. [UNESP] 1-hydroxypyrene Occupational health Sugar-cane workers Urine Validation of analytical methodology |
title_short |
Optimization and validation of analytical methodology for determination of 1-hydroxypyrene in urine of sugar cane workers |
title_full |
Optimization and validation of analytical methodology for determination of 1-hydroxypyrene in urine of sugar cane workers |
title_fullStr |
Optimization and validation of analytical methodology for determination of 1-hydroxypyrene in urine of sugar cane workers |
title_full_unstemmed |
Optimization and validation of analytical methodology for determination of 1-hydroxypyrene in urine of sugar cane workers |
title_sort |
Optimization and validation of analytical methodology for determination of 1-hydroxypyrene in urine of sugar cane workers |
author |
Sakiara, K. A. [UNESP] |
author_facet |
Sakiara, K. A. [UNESP] De Andrade, S. J. [UNESP] Marchi, M. R.R. [UNESP] Vilegas, W. [UNESP] Bosso, R. M.V. Conforti-Froes, N. D.T. |
author_role |
author |
author2 |
De Andrade, S. J. [UNESP] Marchi, M. R.R. [UNESP] Vilegas, W. [UNESP] Bosso, R. M.V. Conforti-Froes, N. D.T. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Sakiara, K. A. [UNESP] De Andrade, S. J. [UNESP] Marchi, M. R.R. [UNESP] Vilegas, W. [UNESP] Bosso, R. M.V. Conforti-Froes, N. D.T. |
dc.subject.por.fl_str_mv |
1-hydroxypyrene Occupational health Sugar-cane workers Urine Validation of analytical methodology |
topic |
1-hydroxypyrene Occupational health Sugar-cane workers Urine Validation of analytical methodology |
description |
This study aimed to optimize and validate an analytical methodology for determination of 1- hydroxypyrene in urine of workers involved in harvesting sugar cane. The method used for determining 1- hydroxypyrene in human urine is the enzymatic hydrolysis, extraction and clean-up by solid phase extraction (SPE) and quantified by high performance liquid chromatography with fluorescence detection (HPLC / Flu). Four types of cartridges were tested to verify the percentage of recovery. Urine of sugar-cane workers (nonsmokers and of both sexes) were collected during the harvest (n = 39) and non-harvest season (n = 34) of cane sugar. The best recovery results were attributed to the C18 cartridges. They presented recovery between 79% and 108%, with a coefficient of variation between 5% and 10%. The limit of quantification was 74 ng of 1- hydroxypyrene per liter of urine. The optimized and validated methodology was used for determination of real samples. The results found in the urine of workers at harvest period ranged from 0.026 to 2.3 mol of 1- hydroxypyrene per mol creatinine. During the non-harvesting season the results ranged from 0.0023 to 0.38 mol of 1-hydroxypyrene per mol creatinine. The validated methodology proved suitable for determination of 1-hydroxypyrene in human urine. The data indicate that there is strong correlation between excretion of 1- hydroxypyrene and the periods during and between harvests of sugar cane. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-01-01 2022-04-29T08:35:05Z 2022-04-29T08:35:05Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.26850/1678-4618EQJ.V35.4.2010.P113-119 Ecletica Quimica, v. 35, n. 4, p. 113-119, 2010. 1678-4618 0100-4670 http://hdl.handle.net/11449/229681 10.26850/1678-4618EQJ.V35.4.2010.P113-119 2-s2.0-85116830704 |
url |
http://dx.doi.org/10.26850/1678-4618EQJ.V35.4.2010.P113-119 http://hdl.handle.net/11449/229681 |
identifier_str_mv |
Ecletica Quimica, v. 35, n. 4, p. 113-119, 2010. 1678-4618 0100-4670 10.26850/1678-4618EQJ.V35.4.2010.P113-119 2-s2.0-85116830704 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
Ecletica Quimica |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
113-119 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808129548748849152 |