Astrocyte intermediate filaments are important markers in olfactory bulb of bovine herpesvirus type 5 natural infections
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://www.abpv.vet.br/upload/documentos/V.3,-N.1,-04-20881_2010_4_29_34_20.pdf http://hdl.handle.net/11449/71681 |
Resumo: | Astroglial cells are the most abundant cells in the mammalian central nervous system, yet our knowledge about their function in bovine Herpesvirus type 5 (BoHV-5) has been limited. The aim of this study was to detect by immunohistochemistry assay the reactive astrocytes for glial fibrilary acidic protein (GFAP) and vimentin (VIM), considered intermediate filaments of the cytoskeleton, localized in olfactory bulb from natural acute cases of BoHV-5 infection. All samples were submitted to virus isolation, real-time polymerase chain reaction (RT-PCR) and in situ hybridization (ISH) technique to confirm the virus transcription and respective genome. Samples were classified into four groups according to the severity of histological lesions. Groups III and IV, which histological lesions were classified as alacia, gliosis, satellitosis, neuronophagia and neuronal necrosis, 35% (± 1.8-2.1) of the inflammatory mononuclear cells, corresponded to CD3 positive lymphocytes. In the same group, 35% (± 1.8) of astrocytes were described as reactive to GFAP and VIM proteins. An agreement of r = 1.0 (P<0.0001) was found between histological lesions, intermediate filaments expression, viral DNA and transcription and CD3 lymphocytes. However, samples with mild histological lesions, 10.8 to 14.2% of astrocytes were classified as reactive to GFAP and VIM filaments. Our findings suggest that GFAP and VIM reactive astrocytes, in primary site of virus replication, seems to play an important role in neurovirulence, in spite of many questions concerning the virus immunopathology remains unclear. |
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Astrocyte intermediate filaments are important markers in olfactory bulb of bovine herpesvirus type 5 natural infectionsBoHV-5Bovine hespesvirusGFAPNeuropathologyVimAlaciaBovinaeBovine herpesvirus 5HerpesviridaeMammaliaAstroglial cells are the most abundant cells in the mammalian central nervous system, yet our knowledge about their function in bovine Herpesvirus type 5 (BoHV-5) has been limited. The aim of this study was to detect by immunohistochemistry assay the reactive astrocytes for glial fibrilary acidic protein (GFAP) and vimentin (VIM), considered intermediate filaments of the cytoskeleton, localized in olfactory bulb from natural acute cases of BoHV-5 infection. All samples were submitted to virus isolation, real-time polymerase chain reaction (RT-PCR) and in situ hybridization (ISH) technique to confirm the virus transcription and respective genome. Samples were classified into four groups according to the severity of histological lesions. Groups III and IV, which histological lesions were classified as alacia, gliosis, satellitosis, neuronophagia and neuronal necrosis, 35% (± 1.8-2.1) of the inflammatory mononuclear cells, corresponded to CD3 positive lymphocytes. In the same group, 35% (± 1.8) of astrocytes were described as reactive to GFAP and VIM proteins. An agreement of r = 1.0 (P<0.0001) was found between histological lesions, intermediate filaments expression, viral DNA and transcription and CD3 lymphocytes. However, samples with mild histological lesions, 10.8 to 14.2% of astrocytes were classified as reactive to GFAP and VIM filaments. Our findings suggest that GFAP and VIM reactive astrocytes, in primary site of virus replication, seems to play an important role in neurovirulence, in spite of many questions concerning the virus immunopathology remains unclear.Laboratório de Virologia Animal Unesp- Universidade Estadual Paulista, Araçatuba, SPLaboratório de Patologia Animal UNESP, Araçatuba, SPInstituto Biológico, São PauloLaboratório de Virologia Animal Unesp- Universidade Estadual Paulista, Araçatuba, SPLaboratório de Patologia Animal UNESP, Araçatuba, SPUniversidade Estadual Paulista (Unesp)Instituto BiológicoGomes, Deriane E. [UNESP]Ferrari, Heitor F. [UNESP]Roncatti, Flávio T.L.B. [UNESP]Paes, Fernanda [UNESP]Cardoso, Lívia S. [UNESP]Perri, Silvia Helena Venturoli [UNESP]Nogueira, Adriana H. C.Luvizotto, Maria Cecília R. [UNESP]Cardoso, Tereza C. [UNESP]2014-05-27T11:24:41Z2014-05-27T11:24:41Z2010-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article17-23http://www.abpv.vet.br/upload/documentos/V.3,-N.1,-04-20881_2010_4_29_34_20.pdfBrazilian Journal of Veterinary Pathology, v. 3, n. 1, p. 17-23, 2010.1983-0246http://hdl.handle.net/11449/716812-s2.0-799524327874584674909952477Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal of Veterinary Pathologyinfo:eu-repo/semantics/openAccess2021-10-22T18:20:40Zoai:repositorio.unesp.br:11449/71681Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:55:11.063406Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Astrocyte intermediate filaments are important markers in olfactory bulb of bovine herpesvirus type 5 natural infections |
title |
Astrocyte intermediate filaments are important markers in olfactory bulb of bovine herpesvirus type 5 natural infections |
spellingShingle |
Astrocyte intermediate filaments are important markers in olfactory bulb of bovine herpesvirus type 5 natural infections Gomes, Deriane E. [UNESP] BoHV-5 Bovine hespesvirus GFAP Neuropathology Vim Alacia Bovinae Bovine herpesvirus 5 Herpesviridae Mammalia |
title_short |
Astrocyte intermediate filaments are important markers in olfactory bulb of bovine herpesvirus type 5 natural infections |
title_full |
Astrocyte intermediate filaments are important markers in olfactory bulb of bovine herpesvirus type 5 natural infections |
title_fullStr |
Astrocyte intermediate filaments are important markers in olfactory bulb of bovine herpesvirus type 5 natural infections |
title_full_unstemmed |
Astrocyte intermediate filaments are important markers in olfactory bulb of bovine herpesvirus type 5 natural infections |
title_sort |
Astrocyte intermediate filaments are important markers in olfactory bulb of bovine herpesvirus type 5 natural infections |
author |
Gomes, Deriane E. [UNESP] |
author_facet |
Gomes, Deriane E. [UNESP] Ferrari, Heitor F. [UNESP] Roncatti, Flávio T.L.B. [UNESP] Paes, Fernanda [UNESP] Cardoso, Lívia S. [UNESP] Perri, Silvia Helena Venturoli [UNESP] Nogueira, Adriana H. C. Luvizotto, Maria Cecília R. [UNESP] Cardoso, Tereza C. [UNESP] |
author_role |
author |
author2 |
Ferrari, Heitor F. [UNESP] Roncatti, Flávio T.L.B. [UNESP] Paes, Fernanda [UNESP] Cardoso, Lívia S. [UNESP] Perri, Silvia Helena Venturoli [UNESP] Nogueira, Adriana H. C. Luvizotto, Maria Cecília R. [UNESP] Cardoso, Tereza C. [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Instituto Biológico |
dc.contributor.author.fl_str_mv |
Gomes, Deriane E. [UNESP] Ferrari, Heitor F. [UNESP] Roncatti, Flávio T.L.B. [UNESP] Paes, Fernanda [UNESP] Cardoso, Lívia S. [UNESP] Perri, Silvia Helena Venturoli [UNESP] Nogueira, Adriana H. C. Luvizotto, Maria Cecília R. [UNESP] Cardoso, Tereza C. [UNESP] |
dc.subject.por.fl_str_mv |
BoHV-5 Bovine hespesvirus GFAP Neuropathology Vim Alacia Bovinae Bovine herpesvirus 5 Herpesviridae Mammalia |
topic |
BoHV-5 Bovine hespesvirus GFAP Neuropathology Vim Alacia Bovinae Bovine herpesvirus 5 Herpesviridae Mammalia |
description |
Astroglial cells are the most abundant cells in the mammalian central nervous system, yet our knowledge about their function in bovine Herpesvirus type 5 (BoHV-5) has been limited. The aim of this study was to detect by immunohistochemistry assay the reactive astrocytes for glial fibrilary acidic protein (GFAP) and vimentin (VIM), considered intermediate filaments of the cytoskeleton, localized in olfactory bulb from natural acute cases of BoHV-5 infection. All samples were submitted to virus isolation, real-time polymerase chain reaction (RT-PCR) and in situ hybridization (ISH) technique to confirm the virus transcription and respective genome. Samples were classified into four groups according to the severity of histological lesions. Groups III and IV, which histological lesions were classified as alacia, gliosis, satellitosis, neuronophagia and neuronal necrosis, 35% (± 1.8-2.1) of the inflammatory mononuclear cells, corresponded to CD3 positive lymphocytes. In the same group, 35% (± 1.8) of astrocytes were described as reactive to GFAP and VIM proteins. An agreement of r = 1.0 (P<0.0001) was found between histological lesions, intermediate filaments expression, viral DNA and transcription and CD3 lymphocytes. However, samples with mild histological lesions, 10.8 to 14.2% of astrocytes were classified as reactive to GFAP and VIM filaments. Our findings suggest that GFAP and VIM reactive astrocytes, in primary site of virus replication, seems to play an important role in neurovirulence, in spite of many questions concerning the virus immunopathology remains unclear. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-05-01 2014-05-27T11:24:41Z 2014-05-27T11:24:41Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.abpv.vet.br/upload/documentos/V.3,-N.1,-04-20881_2010_4_29_34_20.pdf Brazilian Journal of Veterinary Pathology, v. 3, n. 1, p. 17-23, 2010. 1983-0246 http://hdl.handle.net/11449/71681 2-s2.0-79952432787 4584674909952477 |
url |
http://www.abpv.vet.br/upload/documentos/V.3,-N.1,-04-20881_2010_4_29_34_20.pdf http://hdl.handle.net/11449/71681 |
identifier_str_mv |
Brazilian Journal of Veterinary Pathology, v. 3, n. 1, p. 17-23, 2010. 1983-0246 2-s2.0-79952432787 4584674909952477 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Journal of Veterinary Pathology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
17-23 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128874982146048 |