Electroacupuncture increases immunoexpression of CB1 and CB2 receptors in experimental model of inflammatory bone loss
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.bone.2019.05.015 http://hdl.handle.net/11449/189195 |
Resumo: | This study evaluated the participation of CB1 and CB2 receptors in the antiresorptive effect of electroacupuncture (EA) on an experimental model of inflammatory bone loss in rats. 30 rats were divided into five groups: C (control); EP (experimental periodontitis); EA (C+ EA); EP-EA (EP+ EA in the acupoints LI4, LG11, ST36, ST44); EP - EA-sham (EP+ EA in sham acupoints). For the EP groups, a ligature was placed around the right mandibular first molars at day 1. Sessions of EA or EA-sham were assigned every other day. Animals were euthanized at day 11. Histometric analysis was performed to evaluate the percentage of bone area in the furcation area. Immunolabeling patterns in the periodontal tissues and immunofluorescent staining in the trigeminal ganglia and in the trigeminal spinal tract for CB1 and CB2 receptors were performed. It was observed increased bone loss in the furcation in the EP and EP-EA-sham groups, in comparison to the other groups (p < 0.05). Enhanced CB2 immunolabeling was observed in the periodontal tissues in the EP-EA group, when compared to the EP and EP-EA-sham groups (p < 0.05). Increased CB1 immunofluorescent staining was observed in the neural tissues in the EA treated group in comparison with the other groups (p < 0.05), while no expression of CB2 was observed in those regions. Our study showed that in the presence of inflammatory bone disease, EA treatment reduced bone erosion and increased the immunoexpression of CB1 in the neural tissues and CB2 in the periodontal tissues. |
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Electroacupuncture increases immunoexpression of CB1 and CB2 receptors in experimental model of inflammatory bone lossAlveolar bone lossCB1 receptorCB2 receptorChronic periodontitisElectroacupunctureThis study evaluated the participation of CB1 and CB2 receptors in the antiresorptive effect of electroacupuncture (EA) on an experimental model of inflammatory bone loss in rats. 30 rats were divided into five groups: C (control); EP (experimental periodontitis); EA (C+ EA); EP-EA (EP+ EA in the acupoints LI4, LG11, ST36, ST44); EP - EA-sham (EP+ EA in sham acupoints). For the EP groups, a ligature was placed around the right mandibular first molars at day 1. Sessions of EA or EA-sham were assigned every other day. Animals were euthanized at day 11. Histometric analysis was performed to evaluate the percentage of bone area in the furcation area. Immunolabeling patterns in the periodontal tissues and immunofluorescent staining in the trigeminal ganglia and in the trigeminal spinal tract for CB1 and CB2 receptors were performed. It was observed increased bone loss in the furcation in the EP and EP-EA-sham groups, in comparison to the other groups (p < 0.05). Enhanced CB2 immunolabeling was observed in the periodontal tissues in the EP-EA group, when compared to the EP and EP-EA-sham groups (p < 0.05). Increased CB1 immunofluorescent staining was observed in the neural tissues in the EA treated group in comparison with the other groups (p < 0.05), while no expression of CB2 was observed in those regions. Our study showed that in the presence of inflammatory bone disease, EA treatment reduced bone erosion and increased the immunoexpression of CB1 in the neural tissues and CB2 in the periodontal tissues.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação Cearense de Apoio ao Desenvolvimento Científico e TecnológicoDepartment of Clinical Dentistry Graduate Program in Dentistry Faculty of Pharmacy Dentistry and Nursing Federal University of Ceará - UFCDepartment of Morphology Faculty of Medicine Federal University of CearáDepartment of Nursing Faculty of |Pharmacy Dentistry and Nursing Federal University of CearáDivision of Histology Department of Basic Sciences Dental School of Araçatuba São Paulo State University – UNESPDepartment of Oral & Maxillofacial Surgery and Periodontology School of Dentistry of Ribeirao Preto University of Sao Paulo – USPDepartment of Physiology and Pharmacology Faculty of Medicine Federal University of CearáDivision of Histology Department of Basic Sciences Dental School of Araçatuba São Paulo State University – UNESPCNPq: 425181/2016-2Federal University of Ceará - UFCFederal University of CearáUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Alves, Luiza Clertiani VieiraLisboa, Mario Roberto Pontesda Silveira, Helson Freitasde Sousa, Luane MacêdoGusmão, Jonas Nogueira Ferreira MacielDias, Diego Bernarde SouzaErvolino, Edilson [UNESP]Furlaneto, Flávia Aparecida ChavesVale, Mariana LimaGondim, Delane Viana2019-10-06T16:33:03Z2019-10-06T16:33:03Z2019-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article59-66http://dx.doi.org/10.1016/j.bone.2019.05.015Bone, v. 127, p. 59-66.8756-3282http://hdl.handle.net/11449/18919510.1016/j.bone.2019.05.0152-s2.0-8506647665844080955173468460000-0003-4859-0583Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBoneinfo:eu-repo/semantics/openAccess2021-10-22T22:23:36Zoai:repositorio.unesp.br:11449/189195Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:40:51.529772Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Electroacupuncture increases immunoexpression of CB1 and CB2 receptors in experimental model of inflammatory bone loss |
title |
Electroacupuncture increases immunoexpression of CB1 and CB2 receptors in experimental model of inflammatory bone loss |
spellingShingle |
Electroacupuncture increases immunoexpression of CB1 and CB2 receptors in experimental model of inflammatory bone loss Alves, Luiza Clertiani Vieira Alveolar bone loss CB1 receptor CB2 receptor Chronic periodontitis Electroacupuncture |
title_short |
Electroacupuncture increases immunoexpression of CB1 and CB2 receptors in experimental model of inflammatory bone loss |
title_full |
Electroacupuncture increases immunoexpression of CB1 and CB2 receptors in experimental model of inflammatory bone loss |
title_fullStr |
Electroacupuncture increases immunoexpression of CB1 and CB2 receptors in experimental model of inflammatory bone loss |
title_full_unstemmed |
Electroacupuncture increases immunoexpression of CB1 and CB2 receptors in experimental model of inflammatory bone loss |
title_sort |
Electroacupuncture increases immunoexpression of CB1 and CB2 receptors in experimental model of inflammatory bone loss |
author |
Alves, Luiza Clertiani Vieira |
author_facet |
Alves, Luiza Clertiani Vieira Lisboa, Mario Roberto Pontes da Silveira, Helson Freitas de Sousa, Luane Macêdo Gusmão, Jonas Nogueira Ferreira Maciel Dias, Diego Bernarde Souza Ervolino, Edilson [UNESP] Furlaneto, Flávia Aparecida Chaves Vale, Mariana Lima Gondim, Delane Viana |
author_role |
author |
author2 |
Lisboa, Mario Roberto Pontes da Silveira, Helson Freitas de Sousa, Luane Macêdo Gusmão, Jonas Nogueira Ferreira Maciel Dias, Diego Bernarde Souza Ervolino, Edilson [UNESP] Furlaneto, Flávia Aparecida Chaves Vale, Mariana Lima Gondim, Delane Viana |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Federal University of Ceará - UFC Federal University of Ceará Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Alves, Luiza Clertiani Vieira Lisboa, Mario Roberto Pontes da Silveira, Helson Freitas de Sousa, Luane Macêdo Gusmão, Jonas Nogueira Ferreira Maciel Dias, Diego Bernarde Souza Ervolino, Edilson [UNESP] Furlaneto, Flávia Aparecida Chaves Vale, Mariana Lima Gondim, Delane Viana |
dc.subject.por.fl_str_mv |
Alveolar bone loss CB1 receptor CB2 receptor Chronic periodontitis Electroacupuncture |
topic |
Alveolar bone loss CB1 receptor CB2 receptor Chronic periodontitis Electroacupuncture |
description |
This study evaluated the participation of CB1 and CB2 receptors in the antiresorptive effect of electroacupuncture (EA) on an experimental model of inflammatory bone loss in rats. 30 rats were divided into five groups: C (control); EP (experimental periodontitis); EA (C+ EA); EP-EA (EP+ EA in the acupoints LI4, LG11, ST36, ST44); EP - EA-sham (EP+ EA in sham acupoints). For the EP groups, a ligature was placed around the right mandibular first molars at day 1. Sessions of EA or EA-sham were assigned every other day. Animals were euthanized at day 11. Histometric analysis was performed to evaluate the percentage of bone area in the furcation area. Immunolabeling patterns in the periodontal tissues and immunofluorescent staining in the trigeminal ganglia and in the trigeminal spinal tract for CB1 and CB2 receptors were performed. It was observed increased bone loss in the furcation in the EP and EP-EA-sham groups, in comparison to the other groups (p < 0.05). Enhanced CB2 immunolabeling was observed in the periodontal tissues in the EP-EA group, when compared to the EP and EP-EA-sham groups (p < 0.05). Increased CB1 immunofluorescent staining was observed in the neural tissues in the EA treated group in comparison with the other groups (p < 0.05), while no expression of CB2 was observed in those regions. Our study showed that in the presence of inflammatory bone disease, EA treatment reduced bone erosion and increased the immunoexpression of CB1 in the neural tissues and CB2 in the periodontal tissues. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T16:33:03Z 2019-10-06T16:33:03Z 2019-10-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.bone.2019.05.015 Bone, v. 127, p. 59-66. 8756-3282 http://hdl.handle.net/11449/189195 10.1016/j.bone.2019.05.015 2-s2.0-85066476658 4408095517346846 0000-0003-4859-0583 |
url |
http://dx.doi.org/10.1016/j.bone.2019.05.015 http://hdl.handle.net/11449/189195 |
identifier_str_mv |
Bone, v. 127, p. 59-66. 8756-3282 10.1016/j.bone.2019.05.015 2-s2.0-85066476658 4408095517346846 0000-0003-4859-0583 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Bone |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
59-66 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128549778882560 |