Quantification of bovine cytokine gene expression using real-time RT-PCR methodology

Detalhes bibliográficos
Autor(a) principal: Zaros, Lilian Giotto
Data de Publicação: 2007
Outros Autores: Bricarello, Patrízia Ana [UNESP], Amarante, Alessandro Francisco Talamini do [UNESP], Coutinho, Luiz Lehmann
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S1415-47572007000400012
http://hdl.handle.net/11449/18890
Resumo: T cells produce cytokines that affect host response to infection. This paper reports real-time RT-PCR conditions and validation steps for accurate quantification of Bos indicus cytokines, interleukin (IL)-2, IL-4, IL-8, IL12p-35, IL-13, tumoral necrosis factor (TNF)-alpha, interferon (IFN)-gamma, monocyte chemoattractant proteins (MCP)-1 and MCP-2, and the glycoprotein mucin (MUC)-1 in two groups of Nelore cattle, one resistant and the other susceptible to gastrointestinal nematode infections. RPL-19 was shown to be an ideal internal control gene, since its expression was constant across treatments and presented lower variation when compared to the GAPDH gene. The optimized conditions established in the present study can be used to determine the immune response of cattle under different experimental conditions, such as viral, bacterial and parasite infections.
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spelling Quantification of bovine cytokine gene expression using real-time RT-PCR methodologycytokine gene expressionreal-time RT-PCRcattlegastrointestinal nematodesbovine cytokinesT cells produce cytokines that affect host response to infection. This paper reports real-time RT-PCR conditions and validation steps for accurate quantification of Bos indicus cytokines, interleukin (IL)-2, IL-4, IL-8, IL12p-35, IL-13, tumoral necrosis factor (TNF)-alpha, interferon (IFN)-gamma, monocyte chemoattractant proteins (MCP)-1 and MCP-2, and the glycoprotein mucin (MUC)-1 in two groups of Nelore cattle, one resistant and the other susceptible to gastrointestinal nematode infections. RPL-19 was shown to be an ideal internal control gene, since its expression was constant across treatments and presented lower variation when compared to the GAPDH gene. The optimized conditions established in the present study can be used to determine the immune response of cattle under different experimental conditions, such as viral, bacterial and parasite infections.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidade de São Paulo Escola Superior de Agricultura Luiz de Queiroz Departamento de ZootecniaUniversidade Estadual Paulista Departamento de ParasitologiaUniversidade Estadual Paulista Departamento de ParasitologiaSociedade Brasileira de GenéticaUniversidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Zaros, Lilian GiottoBricarello, Patrízia Ana [UNESP]Amarante, Alessandro Francisco Talamini do [UNESP]Coutinho, Luiz Lehmann2014-05-20T13:52:56Z2014-05-20T13:52:56Z2007-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article575-579application/pdfhttp://dx.doi.org/10.1590/S1415-47572007000400012Genetics and Molecular Biology. Sociedade Brasileira de Genética, v. 30, n. 3, p. 575-579, 2007.1415-4757http://hdl.handle.net/11449/1889010.1590/S1415-47572007000400012S1415-47572007000400012S1415-47572007000400012.pdf2677231663329706SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenetics and Molecular Biology1.4930,638info:eu-repo/semantics/openAccess2024-01-14T06:17:53Zoai:repositorio.unesp.br:11449/18890Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:55:04.395082Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Quantification of bovine cytokine gene expression using real-time RT-PCR methodology
title Quantification of bovine cytokine gene expression using real-time RT-PCR methodology
spellingShingle Quantification of bovine cytokine gene expression using real-time RT-PCR methodology
Zaros, Lilian Giotto
cytokine gene expression
real-time RT-PCR
cattle
gastrointestinal nematodes
bovine cytokines
title_short Quantification of bovine cytokine gene expression using real-time RT-PCR methodology
title_full Quantification of bovine cytokine gene expression using real-time RT-PCR methodology
title_fullStr Quantification of bovine cytokine gene expression using real-time RT-PCR methodology
title_full_unstemmed Quantification of bovine cytokine gene expression using real-time RT-PCR methodology
title_sort Quantification of bovine cytokine gene expression using real-time RT-PCR methodology
author Zaros, Lilian Giotto
author_facet Zaros, Lilian Giotto
Bricarello, Patrízia Ana [UNESP]
Amarante, Alessandro Francisco Talamini do [UNESP]
Coutinho, Luiz Lehmann
author_role author
author2 Bricarello, Patrízia Ana [UNESP]
Amarante, Alessandro Francisco Talamini do [UNESP]
Coutinho, Luiz Lehmann
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Zaros, Lilian Giotto
Bricarello, Patrízia Ana [UNESP]
Amarante, Alessandro Francisco Talamini do [UNESP]
Coutinho, Luiz Lehmann
dc.subject.por.fl_str_mv cytokine gene expression
real-time RT-PCR
cattle
gastrointestinal nematodes
bovine cytokines
topic cytokine gene expression
real-time RT-PCR
cattle
gastrointestinal nematodes
bovine cytokines
description T cells produce cytokines that affect host response to infection. This paper reports real-time RT-PCR conditions and validation steps for accurate quantification of Bos indicus cytokines, interleukin (IL)-2, IL-4, IL-8, IL12p-35, IL-13, tumoral necrosis factor (TNF)-alpha, interferon (IFN)-gamma, monocyte chemoattractant proteins (MCP)-1 and MCP-2, and the glycoprotein mucin (MUC)-1 in two groups of Nelore cattle, one resistant and the other susceptible to gastrointestinal nematode infections. RPL-19 was shown to be an ideal internal control gene, since its expression was constant across treatments and presented lower variation when compared to the GAPDH gene. The optimized conditions established in the present study can be used to determine the immune response of cattle under different experimental conditions, such as viral, bacterial and parasite infections.
publishDate 2007
dc.date.none.fl_str_mv 2007-01-01
2014-05-20T13:52:56Z
2014-05-20T13:52:56Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1415-47572007000400012
Genetics and Molecular Biology. Sociedade Brasileira de Genética, v. 30, n. 3, p. 575-579, 2007.
1415-4757
http://hdl.handle.net/11449/18890
10.1590/S1415-47572007000400012
S1415-47572007000400012
S1415-47572007000400012.pdf
2677231663329706
url http://dx.doi.org/10.1590/S1415-47572007000400012
http://hdl.handle.net/11449/18890
identifier_str_mv Genetics and Molecular Biology. Sociedade Brasileira de Genética, v. 30, n. 3, p. 575-579, 2007.
1415-4757
10.1590/S1415-47572007000400012
S1415-47572007000400012
S1415-47572007000400012.pdf
2677231663329706
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Genetics and Molecular Biology
1.493
0,638
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 575-579
application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129473334214656

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