Inflammatory response and macrophage polarization using different physicochemical biomaterials for oral and maxillofacial reconstruction
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.msec.2019.110229 http://hdl.handle.net/11449/198112 |
Resumo: | Knowledge about the action of immune system in the recognition of biomaterials has been extremely helpful when it comes about understanding host response and biomaterials' fate in human body. This study aimed to investigate inflammatory response and macrophage polarization during bone healing process of rat's calvaria critical defects using different bone materials in order to evaluate their influence on bone repair and on the quality of the newly formed bone tissue. Eighty male albinus Wistar rats underwent surgical procedure for the confectioning of a 5-mm diameter bone defect in their right parietal bone, and divided in four groups (n = 20 each), according the biomaterial: AG – Control, particulate intramembranous autogenous bone graft, HA/TCP – particulate biphasic calcium phosphate with HA/TCP (60/40), DBB – particulate deproteinized bovine bone, VC – particulate bioactive vitroceramic. After 3, 7, 21, and 45 days, the specimens were removed and prepared for microcomputed tomography (microCT), light and polarized microscopy, immunohistochemical analysis, and histomorphometry. No significant differences were detected considering percentage of leukocytes among the groups and periods, as well as in relation to immunolabeling for inflammatory (M1) and reparative (M2) macrophages. However, immunolabeling for bone marker indicated a delayed osteoblast differentiation in VC group, resulting in a decrease in mineralized bone matrix parameters in this group, revealed by microCT. In addition, AG and HA/TCP presented a satisfactory bone collagenous content. Despite the distinct origins and physicochemical properties of the tested biomaterials, they presented similar immune-inflammatory responses in the present experimental model, influencing bone-related proteins and bone quality, which must be considered according to their use. |
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Inflammatory response and macrophage polarization using different physicochemical biomaterials for oral and maxillofacial reconstructionBiomaterialsBone substitutesMacrophagesOsteoimmunologyRatsKnowledge about the action of immune system in the recognition of biomaterials has been extremely helpful when it comes about understanding host response and biomaterials' fate in human body. This study aimed to investigate inflammatory response and macrophage polarization during bone healing process of rat's calvaria critical defects using different bone materials in order to evaluate their influence on bone repair and on the quality of the newly formed bone tissue. Eighty male albinus Wistar rats underwent surgical procedure for the confectioning of a 5-mm diameter bone defect in their right parietal bone, and divided in four groups (n = 20 each), according the biomaterial: AG – Control, particulate intramembranous autogenous bone graft, HA/TCP – particulate biphasic calcium phosphate with HA/TCP (60/40), DBB – particulate deproteinized bovine bone, VC – particulate bioactive vitroceramic. After 3, 7, 21, and 45 days, the specimens were removed and prepared for microcomputed tomography (microCT), light and polarized microscopy, immunohistochemical analysis, and histomorphometry. No significant differences were detected considering percentage of leukocytes among the groups and periods, as well as in relation to immunolabeling for inflammatory (M1) and reparative (M2) macrophages. However, immunolabeling for bone marker indicated a delayed osteoblast differentiation in VC group, resulting in a decrease in mineralized bone matrix parameters in this group, revealed by microCT. In addition, AG and HA/TCP presented a satisfactory bone collagenous content. Despite the distinct origins and physicochemical properties of the tested biomaterials, they presented similar immune-inflammatory responses in the present experimental model, influencing bone-related proteins and bone quality, which must be considered according to their use.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Health Sciences Sagrado Coração University – USC, Rua Irmã Arminda 10-50Department of Basic Sciences São Paulo State University (Unesp) School of Dentistry, Rua José Bonifácio 1193Department of Dentistry Endodontics and Dental Materials Bauru School of Dentistry University of São Paulo – FOB/USP, Al. Octávio Pinheiro Brisola, 9-75Research and Education Center for Phototherapy in Health Science (Nupen), Rua Pedro Fernandes Alonso, 766, Jardim AlvoradaDepartment of Basic Sciences São Paulo State University (Unesp) School of Dentistry, Rua José Bonifácio 1193FAPESP: 03762-7FAPESP: 2016Sagrado Coração University – USCUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Research and Education Center for Phototherapy in Health Science (Nupen)Munerato, Marcelo SallesBiguetti, Claudia Cristina [UNESP]Parra da Silva, Raquel Barroso [UNESP]Rodrigues da Silva, Ana Claudia [UNESP]Zucon Bacelar, Ana Carolina [UNESP]Lima da Silva, JordanRondina Couto, Maira CristinaHúngaro Duarte, Marco AntônioSantiago-Junior, Joel FerreiraBossini, Paulo SérgioMatsumoto, Mariza Akemi [UNESP]2020-12-12T00:59:29Z2020-12-12T00:59:29Z2020-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.msec.2019.110229Materials Science and Engineering C, v. 107.1873-01910928-4931http://hdl.handle.net/11449/19811210.1016/j.msec.2019.1102292-s2.0-85074763939Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMaterials Science and Engineering Cinfo:eu-repo/semantics/openAccess2021-10-23T08:53:36Zoai:repositorio.unesp.br:11449/198112Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T08:53:36Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Inflammatory response and macrophage polarization using different physicochemical biomaterials for oral and maxillofacial reconstruction |
title |
Inflammatory response and macrophage polarization using different physicochemical biomaterials for oral and maxillofacial reconstruction |
spellingShingle |
Inflammatory response and macrophage polarization using different physicochemical biomaterials for oral and maxillofacial reconstruction Munerato, Marcelo Salles Biomaterials Bone substitutes Macrophages Osteoimmunology Rats |
title_short |
Inflammatory response and macrophage polarization using different physicochemical biomaterials for oral and maxillofacial reconstruction |
title_full |
Inflammatory response and macrophage polarization using different physicochemical biomaterials for oral and maxillofacial reconstruction |
title_fullStr |
Inflammatory response and macrophage polarization using different physicochemical biomaterials for oral and maxillofacial reconstruction |
title_full_unstemmed |
Inflammatory response and macrophage polarization using different physicochemical biomaterials for oral and maxillofacial reconstruction |
title_sort |
Inflammatory response and macrophage polarization using different physicochemical biomaterials for oral and maxillofacial reconstruction |
author |
Munerato, Marcelo Salles |
author_facet |
Munerato, Marcelo Salles Biguetti, Claudia Cristina [UNESP] Parra da Silva, Raquel Barroso [UNESP] Rodrigues da Silva, Ana Claudia [UNESP] Zucon Bacelar, Ana Carolina [UNESP] Lima da Silva, Jordan Rondina Couto, Maira Cristina Húngaro Duarte, Marco Antônio Santiago-Junior, Joel Ferreira Bossini, Paulo Sérgio Matsumoto, Mariza Akemi [UNESP] |
author_role |
author |
author2 |
Biguetti, Claudia Cristina [UNESP] Parra da Silva, Raquel Barroso [UNESP] Rodrigues da Silva, Ana Claudia [UNESP] Zucon Bacelar, Ana Carolina [UNESP] Lima da Silva, Jordan Rondina Couto, Maira Cristina Húngaro Duarte, Marco Antônio Santiago-Junior, Joel Ferreira Bossini, Paulo Sérgio Matsumoto, Mariza Akemi [UNESP] |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Sagrado Coração University – USC Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) Research and Education Center for Phototherapy in Health Science (Nupen) |
dc.contributor.author.fl_str_mv |
Munerato, Marcelo Salles Biguetti, Claudia Cristina [UNESP] Parra da Silva, Raquel Barroso [UNESP] Rodrigues da Silva, Ana Claudia [UNESP] Zucon Bacelar, Ana Carolina [UNESP] Lima da Silva, Jordan Rondina Couto, Maira Cristina Húngaro Duarte, Marco Antônio Santiago-Junior, Joel Ferreira Bossini, Paulo Sérgio Matsumoto, Mariza Akemi [UNESP] |
dc.subject.por.fl_str_mv |
Biomaterials Bone substitutes Macrophages Osteoimmunology Rats |
topic |
Biomaterials Bone substitutes Macrophages Osteoimmunology Rats |
description |
Knowledge about the action of immune system in the recognition of biomaterials has been extremely helpful when it comes about understanding host response and biomaterials' fate in human body. This study aimed to investigate inflammatory response and macrophage polarization during bone healing process of rat's calvaria critical defects using different bone materials in order to evaluate their influence on bone repair and on the quality of the newly formed bone tissue. Eighty male albinus Wistar rats underwent surgical procedure for the confectioning of a 5-mm diameter bone defect in their right parietal bone, and divided in four groups (n = 20 each), according the biomaterial: AG – Control, particulate intramembranous autogenous bone graft, HA/TCP – particulate biphasic calcium phosphate with HA/TCP (60/40), DBB – particulate deproteinized bovine bone, VC – particulate bioactive vitroceramic. After 3, 7, 21, and 45 days, the specimens were removed and prepared for microcomputed tomography (microCT), light and polarized microscopy, immunohistochemical analysis, and histomorphometry. No significant differences were detected considering percentage of leukocytes among the groups and periods, as well as in relation to immunolabeling for inflammatory (M1) and reparative (M2) macrophages. However, immunolabeling for bone marker indicated a delayed osteoblast differentiation in VC group, resulting in a decrease in mineralized bone matrix parameters in this group, revealed by microCT. In addition, AG and HA/TCP presented a satisfactory bone collagenous content. Despite the distinct origins and physicochemical properties of the tested biomaterials, they presented similar immune-inflammatory responses in the present experimental model, influencing bone-related proteins and bone quality, which must be considered according to their use. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T00:59:29Z 2020-12-12T00:59:29Z 2020-02-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.msec.2019.110229 Materials Science and Engineering C, v. 107. 1873-0191 0928-4931 http://hdl.handle.net/11449/198112 10.1016/j.msec.2019.110229 2-s2.0-85074763939 |
url |
http://dx.doi.org/10.1016/j.msec.2019.110229 http://hdl.handle.net/11449/198112 |
identifier_str_mv |
Materials Science and Engineering C, v. 107. 1873-0191 0928-4931 10.1016/j.msec.2019.110229 2-s2.0-85074763939 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Materials Science and Engineering C |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965605601738752 |