PI3K/AKT signaling drives titanium-induced angiogenic stimulus
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s10856-020-06473-8 http://hdl.handle.net/11449/205769 |
Resumo: | Although osseointegration and clinical success of titanium (Ti)-implanted materials depend on neovascularization in the reactional peri-implant tissue, very little has been achieved considering the Ti-molecules release on the behavior of endothelial cells. To address this issue, we challenged endothelial cells (HUVECs) with Ti-enriched medium obtained from two types of commercial titanium surfaces [presenting or not dual-acid etching (DAE)] up to 72 h to allow molecular machinery analysis. Our data show that the Ti-enriched medium provokes significant stimulus of angiogenesis-related machinery in endothelial cells by upexpressing VEGFR1, VEGFR2, VEGF, eNOS, and iNOS genes, while the PI3K/Akt signaling pathway was also significantly enhanced. As PI3K/AKT signaling was related to angiogenesis in response to vascular endothelial growth factor (VEGF), we addressed the importance of PI3K/Akt upon Ti-enriched medium responses by concomitantly treating the cells with wortmannin, a well-known PI3K inhibitor. Wortmannin suppressed the angiogenic factors, because VEGF, VEGFR1, and eNOS genes were downregulated in those cells, highlighting the importance of PI3K/AKT signaling on driving angiogenic phenotype and angiogenesis performance within the peri-implant tissue reaction. In conjunction, these data reinforce that titanium-implantable devices modify the metabolism of surrounding cells, such as endothelial cells, probably coupling osteogenesis and angiogenesis processes in peri-implant tissue and then contributing to successfully osseointegration of biomedical titanium-based devices. [Figure not available: see fulltext.]. |
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PI3K/AKT signaling drives titanium-induced angiogenic stimulusAlthough osseointegration and clinical success of titanium (Ti)-implanted materials depend on neovascularization in the reactional peri-implant tissue, very little has been achieved considering the Ti-molecules release on the behavior of endothelial cells. To address this issue, we challenged endothelial cells (HUVECs) with Ti-enriched medium obtained from two types of commercial titanium surfaces [presenting or not dual-acid etching (DAE)] up to 72 h to allow molecular machinery analysis. Our data show that the Ti-enriched medium provokes significant stimulus of angiogenesis-related machinery in endothelial cells by upexpressing VEGFR1, VEGFR2, VEGF, eNOS, and iNOS genes, while the PI3K/Akt signaling pathway was also significantly enhanced. As PI3K/AKT signaling was related to angiogenesis in response to vascular endothelial growth factor (VEGF), we addressed the importance of PI3K/Akt upon Ti-enriched medium responses by concomitantly treating the cells with wortmannin, a well-known PI3K inhibitor. Wortmannin suppressed the angiogenic factors, because VEGF, VEGFR1, and eNOS genes were downregulated in those cells, highlighting the importance of PI3K/AKT signaling on driving angiogenic phenotype and angiogenesis performance within the peri-implant tissue reaction. In conjunction, these data reinforce that titanium-implantable devices modify the metabolism of surrounding cells, such as endothelial cells, probably coupling osteogenesis and angiogenesis processes in peri-implant tissue and then contributing to successfully osseointegration of biomedical titanium-based devices. [Figure not available: see fulltext.].Institute of Biosciences of Botucatu Department of Chemical and Biological Sciences UNESP – São Paulo State UniversityInstitute of Biosciences of Botucatu Department of Chemical and Biological Sciences UNESP – São Paulo State UniversityUniversidade Estadual Paulista (Unesp)Martins, Bruna Rodrigues [UNESP]Pinto, Thais Silva [UNESP]da Costa Fernandes, Célio Junior [UNESP]Bezerra, Fábio [UNESP]Zambuzzi, Willian Fernando [UNESP]2021-06-25T10:20:57Z2021-06-25T10:20:57Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1007/s10856-020-06473-8Journal of Materials Science: Materials in Medicine, v. 32, n. 1, 2021.1573-48380957-4530http://hdl.handle.net/11449/20576910.1007/s10856-020-06473-82-s2.0-85099749703Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Materials Science: Materials in Medicineinfo:eu-repo/semantics/openAccess2021-10-22T17:11:43Zoai:repositorio.unesp.br:11449/205769Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-22T17:11:43Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
PI3K/AKT signaling drives titanium-induced angiogenic stimulus |
title |
PI3K/AKT signaling drives titanium-induced angiogenic stimulus |
spellingShingle |
PI3K/AKT signaling drives titanium-induced angiogenic stimulus Martins, Bruna Rodrigues [UNESP] |
title_short |
PI3K/AKT signaling drives titanium-induced angiogenic stimulus |
title_full |
PI3K/AKT signaling drives titanium-induced angiogenic stimulus |
title_fullStr |
PI3K/AKT signaling drives titanium-induced angiogenic stimulus |
title_full_unstemmed |
PI3K/AKT signaling drives titanium-induced angiogenic stimulus |
title_sort |
PI3K/AKT signaling drives titanium-induced angiogenic stimulus |
author |
Martins, Bruna Rodrigues [UNESP] |
author_facet |
Martins, Bruna Rodrigues [UNESP] Pinto, Thais Silva [UNESP] da Costa Fernandes, Célio Junior [UNESP] Bezerra, Fábio [UNESP] Zambuzzi, Willian Fernando [UNESP] |
author_role |
author |
author2 |
Pinto, Thais Silva [UNESP] da Costa Fernandes, Célio Junior [UNESP] Bezerra, Fábio [UNESP] Zambuzzi, Willian Fernando [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Martins, Bruna Rodrigues [UNESP] Pinto, Thais Silva [UNESP] da Costa Fernandes, Célio Junior [UNESP] Bezerra, Fábio [UNESP] Zambuzzi, Willian Fernando [UNESP] |
description |
Although osseointegration and clinical success of titanium (Ti)-implanted materials depend on neovascularization in the reactional peri-implant tissue, very little has been achieved considering the Ti-molecules release on the behavior of endothelial cells. To address this issue, we challenged endothelial cells (HUVECs) with Ti-enriched medium obtained from two types of commercial titanium surfaces [presenting or not dual-acid etching (DAE)] up to 72 h to allow molecular machinery analysis. Our data show that the Ti-enriched medium provokes significant stimulus of angiogenesis-related machinery in endothelial cells by upexpressing VEGFR1, VEGFR2, VEGF, eNOS, and iNOS genes, while the PI3K/Akt signaling pathway was also significantly enhanced. As PI3K/AKT signaling was related to angiogenesis in response to vascular endothelial growth factor (VEGF), we addressed the importance of PI3K/Akt upon Ti-enriched medium responses by concomitantly treating the cells with wortmannin, a well-known PI3K inhibitor. Wortmannin suppressed the angiogenic factors, because VEGF, VEGFR1, and eNOS genes were downregulated in those cells, highlighting the importance of PI3K/AKT signaling on driving angiogenic phenotype and angiogenesis performance within the peri-implant tissue reaction. In conjunction, these data reinforce that titanium-implantable devices modify the metabolism of surrounding cells, such as endothelial cells, probably coupling osteogenesis and angiogenesis processes in peri-implant tissue and then contributing to successfully osseointegration of biomedical titanium-based devices. [Figure not available: see fulltext.]. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T10:20:57Z 2021-06-25T10:20:57Z 2021-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s10856-020-06473-8 Journal of Materials Science: Materials in Medicine, v. 32, n. 1, 2021. 1573-4838 0957-4530 http://hdl.handle.net/11449/205769 10.1007/s10856-020-06473-8 2-s2.0-85099749703 |
url |
http://dx.doi.org/10.1007/s10856-020-06473-8 http://hdl.handle.net/11449/205769 |
identifier_str_mv |
Journal of Materials Science: Materials in Medicine, v. 32, n. 1, 2021. 1573-4838 0957-4530 10.1007/s10856-020-06473-8 2-s2.0-85099749703 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Materials Science: Materials in Medicine |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965367873830912 |