Successive seminal collections from Leiarius marmoratus during the reproductive period
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.anireprosci.2021.106852 http://hdl.handle.net/11449/222488 |
Resumo: | This study aim was to verify whether milt quality of male Leiarius marmoratus is maintained among successive samples collected during the same reproductive period. Ten reproductively mature males were used to evaluate four successive sperm samples collected at 10-day intervals. For these collections, seven males were injected with carp pituitary homogenate (CPH) at a dosage of 3.0 mg/kg body weight, in two applications (30% and 70%), at an interval of 10 h. The other three males were administered only saline (control). Injection with CPH or saline occurred prior to each of the four collections. Only one male from the control group released a small volume of milt (0.33 mL), and only during the first collection period. Of the seven males treated with CPH, five released milt during all four collections. Milt volume of the first sample collected (0.63 mL) did not differ from that of other samples (0.59–1.38 mL; P > 0.05). Sperm concentration was greater in the first samples collected (1.98 × 109 spermatozoa/mL) compared to the other samples (0.35 × 109 at 0.92 × 109 spermatozoa/mL; P < 0.05). Sperm motility, curvilinear velocity, straightness, and morphological normality did not differ among the consecutive samples (P > 0.05). Average path velocity, straight-line velocity, oscillation, linearity, progression, and membrane integrity decreased slightly in the samples collected subsequent to the first sample (P > 0.05). In conclusion, milt quality decreased among successive collections; however, quality of all samples from all collections was sufficient for use for fertilization of oocytes. |
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Repositório Institucional da UNESP |
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spelling |
Successive seminal collections from Leiarius marmoratus during the reproductive periodCarp pituitary homogenateComputer-assisted sperm analysisFish reproductionRheophilic fishSuccessive seminal collectionsThis study aim was to verify whether milt quality of male Leiarius marmoratus is maintained among successive samples collected during the same reproductive period. Ten reproductively mature males were used to evaluate four successive sperm samples collected at 10-day intervals. For these collections, seven males were injected with carp pituitary homogenate (CPH) at a dosage of 3.0 mg/kg body weight, in two applications (30% and 70%), at an interval of 10 h. The other three males were administered only saline (control). Injection with CPH or saline occurred prior to each of the four collections. Only one male from the control group released a small volume of milt (0.33 mL), and only during the first collection period. Of the seven males treated with CPH, five released milt during all four collections. Milt volume of the first sample collected (0.63 mL) did not differ from that of other samples (0.59–1.38 mL; P > 0.05). Sperm concentration was greater in the first samples collected (1.98 × 109 spermatozoa/mL) compared to the other samples (0.35 × 109 at 0.92 × 109 spermatozoa/mL; P < 0.05). Sperm motility, curvilinear velocity, straightness, and morphological normality did not differ among the consecutive samples (P > 0.05). Average path velocity, straight-line velocity, oscillation, linearity, progression, and membrane integrity decreased slightly in the samples collected subsequent to the first sample (P > 0.05). In conclusion, milt quality decreased among successive collections; however, quality of all samples from all collections was sufficient for use for fertilization of oocytes.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Animal Science Graduate Program Faculty of Veterinary Medicine and Animal Science FAMEZ Mato Grosso do Sul Federal University – UFMSCAUNESPUNESP Fishery Engineering CourseAquam Research Group Animal Science Research Program Federal University of Rio Grande do Sul – UFRGSPiraí Fish-farmingCAUNESPUNESP Fishery Engineering CourseCAPES: 001Universidade Federal de Mato Grosso do Sul (UFMS)Universidade Estadual Paulista (UNESP)Federal University of Rio Grande do Sul – UFRGSPiraí Fish-farmingSpica, Louise NexSanches, Eduardo Antônio [UNESP]Junior, Danilo Pedro StreitFilho, Ruy Alberto Caetano Corrêade Oliveira Brasileiro, LucasRodrigues, Rômulo BatistaKasai, Rodrigo Yutaka DichoffPovh, Jayme Aparecido2022-04-28T19:44:55Z2022-04-28T19:44:55Z2021-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.anireprosci.2021.106852Animal Reproduction Science, v. 234.0378-4320http://hdl.handle.net/11449/22248810.1016/j.anireprosci.2021.1068522-s2.0-85115770787Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAnimal Reproduction Scienceinfo:eu-repo/semantics/openAccess2022-04-28T19:44:55Zoai:repositorio.unesp.br:11449/222488Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:21:21.436226Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Successive seminal collections from Leiarius marmoratus during the reproductive period |
title |
Successive seminal collections from Leiarius marmoratus during the reproductive period |
spellingShingle |
Successive seminal collections from Leiarius marmoratus during the reproductive period Spica, Louise Nex Carp pituitary homogenate Computer-assisted sperm analysis Fish reproduction Rheophilic fish Successive seminal collections |
title_short |
Successive seminal collections from Leiarius marmoratus during the reproductive period |
title_full |
Successive seminal collections from Leiarius marmoratus during the reproductive period |
title_fullStr |
Successive seminal collections from Leiarius marmoratus during the reproductive period |
title_full_unstemmed |
Successive seminal collections from Leiarius marmoratus during the reproductive period |
title_sort |
Successive seminal collections from Leiarius marmoratus during the reproductive period |
author |
Spica, Louise Nex |
author_facet |
Spica, Louise Nex Sanches, Eduardo Antônio [UNESP] Junior, Danilo Pedro Streit Filho, Ruy Alberto Caetano Corrêa de Oliveira Brasileiro, Lucas Rodrigues, Rômulo Batista Kasai, Rodrigo Yutaka Dichoff Povh, Jayme Aparecido |
author_role |
author |
author2 |
Sanches, Eduardo Antônio [UNESP] Junior, Danilo Pedro Streit Filho, Ruy Alberto Caetano Corrêa de Oliveira Brasileiro, Lucas Rodrigues, Rômulo Batista Kasai, Rodrigo Yutaka Dichoff Povh, Jayme Aparecido |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de Mato Grosso do Sul (UFMS) Universidade Estadual Paulista (UNESP) Federal University of Rio Grande do Sul – UFRGS Piraí Fish-farming |
dc.contributor.author.fl_str_mv |
Spica, Louise Nex Sanches, Eduardo Antônio [UNESP] Junior, Danilo Pedro Streit Filho, Ruy Alberto Caetano Corrêa de Oliveira Brasileiro, Lucas Rodrigues, Rômulo Batista Kasai, Rodrigo Yutaka Dichoff Povh, Jayme Aparecido |
dc.subject.por.fl_str_mv |
Carp pituitary homogenate Computer-assisted sperm analysis Fish reproduction Rheophilic fish Successive seminal collections |
topic |
Carp pituitary homogenate Computer-assisted sperm analysis Fish reproduction Rheophilic fish Successive seminal collections |
description |
This study aim was to verify whether milt quality of male Leiarius marmoratus is maintained among successive samples collected during the same reproductive period. Ten reproductively mature males were used to evaluate four successive sperm samples collected at 10-day intervals. For these collections, seven males were injected with carp pituitary homogenate (CPH) at a dosage of 3.0 mg/kg body weight, in two applications (30% and 70%), at an interval of 10 h. The other three males were administered only saline (control). Injection with CPH or saline occurred prior to each of the four collections. Only one male from the control group released a small volume of milt (0.33 mL), and only during the first collection period. Of the seven males treated with CPH, five released milt during all four collections. Milt volume of the first sample collected (0.63 mL) did not differ from that of other samples (0.59–1.38 mL; P > 0.05). Sperm concentration was greater in the first samples collected (1.98 × 109 spermatozoa/mL) compared to the other samples (0.35 × 109 at 0.92 × 109 spermatozoa/mL; P < 0.05). Sperm motility, curvilinear velocity, straightness, and morphological normality did not differ among the consecutive samples (P > 0.05). Average path velocity, straight-line velocity, oscillation, linearity, progression, and membrane integrity decreased slightly in the samples collected subsequent to the first sample (P > 0.05). In conclusion, milt quality decreased among successive collections; however, quality of all samples from all collections was sufficient for use for fertilization of oocytes. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-11-01 2022-04-28T19:44:55Z 2022-04-28T19:44:55Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.anireprosci.2021.106852 Animal Reproduction Science, v. 234. 0378-4320 http://hdl.handle.net/11449/222488 10.1016/j.anireprosci.2021.106852 2-s2.0-85115770787 |
url |
http://dx.doi.org/10.1016/j.anireprosci.2021.106852 http://hdl.handle.net/11449/222488 |
identifier_str_mv |
Animal Reproduction Science, v. 234. 0378-4320 10.1016/j.anireprosci.2021.106852 2-s2.0-85115770787 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Animal Reproduction Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129192248737792 |