Studies of adsorptive capacity of bacterial β-glucosidases on lignocresol aiming the enzymatic recycling in bioprocesses
Autor(a) principal: | |
---|---|
Data de Publicação: | 2019 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.btre.2019.e00326 http://hdl.handle.net/11449/187492 |
Resumo: | Enzymes are essential in many biological processes, including second-generation ethanol production. However, enzymes are one of the main expenses for the industrial process in these days. Several studies have been done to maximize cost savings, however, many processes are still economically infeasible. In this study, we report the synthesis of a suspension of lignocresol for recycling or reuse of enzymes in bioprocesses. In this way, it was performed the adsorption assays between lignocresol and β-glucosidases from Thermotoga petrophila, belonging to the families GH1 and GH3, for the development of a lignocresol-enzyme complex. Our results show that lignocresol maintains greater adsorptive capacity for β-glucosidases than lignin. This capacity can be explained both by its great hydrophobicity and also by electrostatic characteristics. Therefore, all these results demonstrate good adsorption of the enzymes to the lignocresol, demonstrating great potential for enzymatic recycling. |
id |
UNSP_5a40f69dcddf333c34593c979c17ef62 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/187492 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Studies of adsorptive capacity of bacterial β-glucosidases on lignocresol aiming the enzymatic recycling in bioprocessesAdsorptionLigninLignocresolβ-GlucosidaseEnzymes are essential in many biological processes, including second-generation ethanol production. However, enzymes are one of the main expenses for the industrial process in these days. Several studies have been done to maximize cost savings, however, many processes are still economically infeasible. In this study, we report the synthesis of a suspension of lignocresol for recycling or reuse of enzymes in bioprocesses. In this way, it was performed the adsorption assays between lignocresol and β-glucosidases from Thermotoga petrophila, belonging to the families GH1 and GH3, for the development of a lignocresol-enzyme complex. Our results show that lignocresol maintains greater adsorptive capacity for β-glucosidases than lignin. This capacity can be explained both by its great hydrophobicity and also by electrostatic characteristics. Therefore, all these results demonstrate good adsorption of the enzymes to the lignocresol, demonstrating great potential for enzymatic recycling.São Paulo State University - Unesp Department of Physics and Biophysics Institute of BiosciencesCenter for Natural and Human Sciences (CCNH) Federal University of ABC (UFABC)São Paulo State University - Unesp Department of Physics and Biophysics Institute of BiosciencesUniversidade Estadual Paulista (Unesp)Universidade Federal do ABC (UFABC)Barduco Ferreira, Mariana T. [UNESP]Zuccari, Thiago B.B. [UNESP]Garcia, Waniusde Oliveira Neto, Mario [UNESP]2019-10-06T15:37:45Z2019-10-06T15:37:45Z2019-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.btre.2019.e00326Biotechnology Reports, v. 23.2215-017Xhttp://hdl.handle.net/11449/18749210.1016/j.btre.2019.e003262-s2.0-85063462099Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiotechnology Reportsinfo:eu-repo/semantics/openAccess2021-10-23T20:19:17Zoai:repositorio.unesp.br:11449/187492Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T20:19:17Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Studies of adsorptive capacity of bacterial β-glucosidases on lignocresol aiming the enzymatic recycling in bioprocesses |
title |
Studies of adsorptive capacity of bacterial β-glucosidases on lignocresol aiming the enzymatic recycling in bioprocesses |
spellingShingle |
Studies of adsorptive capacity of bacterial β-glucosidases on lignocresol aiming the enzymatic recycling in bioprocesses Barduco Ferreira, Mariana T. [UNESP] Adsorption Lignin Lignocresol β-Glucosidase |
title_short |
Studies of adsorptive capacity of bacterial β-glucosidases on lignocresol aiming the enzymatic recycling in bioprocesses |
title_full |
Studies of adsorptive capacity of bacterial β-glucosidases on lignocresol aiming the enzymatic recycling in bioprocesses |
title_fullStr |
Studies of adsorptive capacity of bacterial β-glucosidases on lignocresol aiming the enzymatic recycling in bioprocesses |
title_full_unstemmed |
Studies of adsorptive capacity of bacterial β-glucosidases on lignocresol aiming the enzymatic recycling in bioprocesses |
title_sort |
Studies of adsorptive capacity of bacterial β-glucosidases on lignocresol aiming the enzymatic recycling in bioprocesses |
author |
Barduco Ferreira, Mariana T. [UNESP] |
author_facet |
Barduco Ferreira, Mariana T. [UNESP] Zuccari, Thiago B.B. [UNESP] Garcia, Wanius de Oliveira Neto, Mario [UNESP] |
author_role |
author |
author2 |
Zuccari, Thiago B.B. [UNESP] Garcia, Wanius de Oliveira Neto, Mario [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Federal do ABC (UFABC) |
dc.contributor.author.fl_str_mv |
Barduco Ferreira, Mariana T. [UNESP] Zuccari, Thiago B.B. [UNESP] Garcia, Wanius de Oliveira Neto, Mario [UNESP] |
dc.subject.por.fl_str_mv |
Adsorption Lignin Lignocresol β-Glucosidase |
topic |
Adsorption Lignin Lignocresol β-Glucosidase |
description |
Enzymes are essential in many biological processes, including second-generation ethanol production. However, enzymes are one of the main expenses for the industrial process in these days. Several studies have been done to maximize cost savings, however, many processes are still economically infeasible. In this study, we report the synthesis of a suspension of lignocresol for recycling or reuse of enzymes in bioprocesses. In this way, it was performed the adsorption assays between lignocresol and β-glucosidases from Thermotoga petrophila, belonging to the families GH1 and GH3, for the development of a lignocresol-enzyme complex. Our results show that lignocresol maintains greater adsorptive capacity for β-glucosidases than lignin. This capacity can be explained both by its great hydrophobicity and also by electrostatic characteristics. Therefore, all these results demonstrate good adsorption of the enzymes to the lignocresol, demonstrating great potential for enzymatic recycling. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T15:37:45Z 2019-10-06T15:37:45Z 2019-09-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.btre.2019.e00326 Biotechnology Reports, v. 23. 2215-017X http://hdl.handle.net/11449/187492 10.1016/j.btre.2019.e00326 2-s2.0-85063462099 |
url |
http://dx.doi.org/10.1016/j.btre.2019.e00326 http://hdl.handle.net/11449/187492 |
identifier_str_mv |
Biotechnology Reports, v. 23. 2215-017X 10.1016/j.btre.2019.e00326 2-s2.0-85063462099 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Biotechnology Reports |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803649811392495616 |