Bases fisiológicas para a conservação a longo prazo de sementes Cariniana legalis (Mart.) O. Kuntze

• Autor(a) principal: Abreu, Daniela Cleide Azevedo de [UNESP]
• Data de Publicação: 2009
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/105109
• topic: Sementes - Armazenamento; Sementes - Recursos de germoplasma - Criopreservação; Agua - Composição; Jequitibá-rosa; Semente florestal; Soluções salinas saturadas; Storage; Cryopreservation; Forest seed; Saturated saline solutions; Moisture content

The understanding of the physiological behavior of seeds is essential to define the appropriated safe storage technique. However, one of the main problems, for many Brazilian native forest species, is the lack of accurate information about this issue. The seeds moisture content and storage temperature are crucial factors for the preservation of the physiological quality of the seeds. The general objective of the present work was to study the possibility of a long term conservation of Cariniana legalis (Mart.) O. Kuntze seeds, in a germplasm bank. The specific objectives were: (a) study the seeds tolerance to dehydration and hydration using saturated saline solutions; (b) classify the physiological behavior of the seeds regarding the storage and (c) evaluate the physiological quality of the seeds with different moisture contents for 360 days in freezer (-20ºC) and liquid nitrogen (-196ºC). The results showed that (a) the construction of the isotherm sorption with saturated saline solutions was efficient to determine different levels of water balance; (b) seeds tolerate dehydration and negative temperature, presenting an orthodox behavior; (c) the most suitable moisture content for seed storage in freezer and liquid nitrogen was of 3,7% (d) the seeds stored with this moisture content were better preserved in liquid nitrogen; (e) the cryopreservation of C. legalis dried seeds showed the possibility of long-term storage in germplasm banks.