Comunidades de fungos em jardins de formigas cortadeiras com diferentes hábitos de forrageamento

• Autor(a) principal: Pereira, Jaqueline Silva [UNESP]
• Data de Publicação: 2014
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/110922
• topic: Ants; Formiga; Formiga-cortadeira; Fungos; Animais - Alimentos; Substratos; Mutualismo

Leaf-cutting ants maintain a mutualistic relationship with fungi cultivated for food. In addition to the ant fungal cultivar, several studies reported the presence of other fungi in the fungus gardens of these insects. To understand the factors that drive the interaction of such alien fungi within fungus gardens, we evaluated the fungal communities associated with two leaf-cutting ant species that exhibit distinct foraging behaviors. Using culture-dependent techniques coupled with a polyphasic approach of species identification, we isolated fungi from garden fragments of eight nests of Atta sexdens rubropilosa (a dicot-cutting ant) and eight nests of Atta capiguara (a grass-cutting ant). All nests were sampled in two consecutive periods (2012 and 2013) in a private farm near to Botucatu, SP. Of the 1,014 garden fragments sampled from both ant species, 623 (61.4 %) were positive for fungi other than the ant cultivar. After morphotyping and DNA sequencing, we obtained 624 fungal strains comprising 61 genera, 135 species and 10 unidentified fungi. Trichoderma spirale (18.5%), Trichosporon chiarellii (12.2%) and Penicillium citrinum (11.7%) were the prevalent species regarding the total number of isolates. The remaining species were not abundant as well, including the specialized fungal parasite Escovopsis sp. Based on ecological metrics our analyses revealed similar richness and diversity of fungal species in gardens of both ants, however, conspicuous differences were observed in fungal species composition between fungus gardens of A. sexdens rubropilosa and A. capiguara. Principal coordinate analysis (PCoA) suggests such differences are related to the type of substrate foraged by workers and the distinct behaviors between ant species related to substrate preparation before incorporation to the fungus garden. Besides substrate type, additional factors such as collecting period may also explain the observed variation in community structure...