In vitro propagation of Maytenus ilicifolia (Celastraceae) as potential source for antitumoral and antioxidant quinomethide triterpenes production. A rapid quantitative method for their analysis by reverse-phase high-performance liquid chromatography

Detalhes bibliográficos
Autor(a) principal: Buffa Filho, Waldemar [UNESP]
Data de Publicação: 2004
Outros Autores: Bolzani, Vanderlan da Silva [UNESP], Furlan, Maysa [UNESP], Vaz Pereira, Sarazete Izidia, Pereira, Ana Maria Soares, Françab, Suzelei Castro
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
DOI: 10.3998/ark.5550190.0005.617
Texto Completo: http://www.arkat-usa.org/get-file/19210/
http://dx.doi.org/10.3998/ark.5550190.0005.617
http://hdl.handle.net/11449/67879
Resumo: Cell culture of Maytenus ilicifolia were established in order to produce and to quantify the antitumoral and antioxidant quinonemethide triterpenes. In vitro calli were induced from leaf explants of native plants and cultured in semi-solid medium under controlled conditions of humidity, temperature and photoperiod. The quinonemethide triterpenes showed maximum accumulation in the logarithmic phase growth of the cell culture. A rapid, sensitive and reliable reverse-phase HPLC method was used for quantitative determination of the antitumoral and antioxidant quinonemethide triterpenes, 22β-hydroxymaytenin and maytenin in callus of Maytenus ilicifolia. Well resolved peaks with good detection response and linearity in the range 1.0 - 100 μg/mL were obtained. This quantitative work was performed by an external standard method.
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spelling In vitro propagation of Maytenus ilicifolia (Celastraceae) as potential source for antitumoral and antioxidant quinomethide triterpenes production. A rapid quantitative method for their analysis by reverse-phase high-performance liquid chromatographyin vitro system, CelastraceaeMaytenus ilicifoliaQuantitative determinationQuinonemethide triterpenes, Reverse-phase high-performance liquid chromatography22beta hydroxymayteninantineoplastic agentantioxidantmayteninmaytenus ilicifolia extractplant extractquinone derivativetriterpene derivativeunclassified drugbioaccumulationCelastraceaecell culturedrug potencydrug synthesisexplanthumidityin vitro studyMaytenusphotosynthesisplant cellplant leafquantitative analysisreliabilityreversed phase high performance liquid chromatographystandardtechniquetemperature dependenceCell culture of Maytenus ilicifolia were established in order to produce and to quantify the antitumoral and antioxidant quinonemethide triterpenes. In vitro calli were induced from leaf explants of native plants and cultured in semi-solid medium under controlled conditions of humidity, temperature and photoperiod. The quinonemethide triterpenes showed maximum accumulation in the logarithmic phase growth of the cell culture. A rapid, sensitive and reliable reverse-phase HPLC method was used for quantitative determination of the antitumoral and antioxidant quinonemethide triterpenes, 22β-hydroxymaytenin and maytenin in callus of Maytenus ilicifolia. Well resolved peaks with good detection response and linearity in the range 1.0 - 100 μg/mL were obtained. This quantitative work was performed by an external standard method.Núcleo de Bioensaio Biossintese Ecofisiologia P. Universidade Estadual Paulista, CP. 355, 14801-970, Araraquara-SPDepartamento de Biotecnologia UNAERP, 14096-380, Ribeirão Preto-SPNúcleo de Bioensaio Biossintese Ecofisiologia P. Universidade Estadual Paulista, CP. 355, 14801-970, Araraquara-SPUniversidade Estadual Paulista (Unesp)UNAERPBuffa Filho, Waldemar [UNESP]Bolzani, Vanderlan da Silva [UNESP]Furlan, Maysa [UNESP]Vaz Pereira, Sarazete IzidiaPereira, Ana Maria SoaresFrançab, Suzelei Castro2014-05-27T11:21:09Z2014-05-27T11:21:09Z2004-09-19info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article137-146application/pdfhttp://www.arkat-usa.org/get-file/19210/http://dx.doi.org/10.3998/ark.5550190.0005.617Arkivoc, v. 2004, n. 6, p. 137-146, 2004.1424-6376http://hdl.handle.net/11449/6787910.3998/ark.5550190.0005.617WOS:0002241531000172-s2.0-5444226445WOS000224153100017.pdf1308042794786872Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArkivocinfo:eu-repo/semantics/openAccess2024-09-27T14:05:35Zoai:repositorio.unesp.br:11449/67879Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-27T14:05:35Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv In vitro propagation of Maytenus ilicifolia (Celastraceae) as potential source for antitumoral and antioxidant quinomethide triterpenes production. A rapid quantitative method for their analysis by reverse-phase high-performance liquid chromatography
title In vitro propagation of Maytenus ilicifolia (Celastraceae) as potential source for antitumoral and antioxidant quinomethide triterpenes production. A rapid quantitative method for their analysis by reverse-phase high-performance liquid chromatography
spellingShingle In vitro propagation of Maytenus ilicifolia (Celastraceae) as potential source for antitumoral and antioxidant quinomethide triterpenes production. A rapid quantitative method for their analysis by reverse-phase high-performance liquid chromatography
In vitro propagation of Maytenus ilicifolia (Celastraceae) as potential source for antitumoral and antioxidant quinomethide triterpenes production. A rapid quantitative method for their analysis by reverse-phase high-performance liquid chromatography
Buffa Filho, Waldemar [UNESP]
in vitro system, Celastraceae
Maytenus ilicifolia
Quantitative determination
Quinonemethide triterpenes, Reverse-phase high-performance liquid chromatography
22beta hydroxymaytenin
antineoplastic agent
antioxidant
maytenin
maytenus ilicifolia extract
plant extract
quinone derivative
triterpene derivative
unclassified drug
bioaccumulation
Celastraceae
cell culture
drug potency
drug synthesis
explant
humidity
in vitro study
Maytenus
photosynthesis
plant cell
plant leaf
quantitative analysis
reliability
reversed phase high performance liquid chromatography
standard
technique
temperature dependence
Buffa Filho, Waldemar [UNESP]
in vitro system, Celastraceae
Maytenus ilicifolia
Quantitative determination
Quinonemethide triterpenes, Reverse-phase high-performance liquid chromatography
22beta hydroxymaytenin
antineoplastic agent
antioxidant
maytenin
maytenus ilicifolia extract
plant extract
quinone derivative
triterpene derivative
unclassified drug
bioaccumulation
Celastraceae
cell culture
drug potency
drug synthesis
explant
humidity
in vitro study
Maytenus
photosynthesis
plant cell
plant leaf
quantitative analysis
reliability
reversed phase high performance liquid chromatography
standard
technique
temperature dependence
title_short In vitro propagation of Maytenus ilicifolia (Celastraceae) as potential source for antitumoral and antioxidant quinomethide triterpenes production. A rapid quantitative method for their analysis by reverse-phase high-performance liquid chromatography
title_full In vitro propagation of Maytenus ilicifolia (Celastraceae) as potential source for antitumoral and antioxidant quinomethide triterpenes production. A rapid quantitative method for their analysis by reverse-phase high-performance liquid chromatography
title_fullStr In vitro propagation of Maytenus ilicifolia (Celastraceae) as potential source for antitumoral and antioxidant quinomethide triterpenes production. A rapid quantitative method for their analysis by reverse-phase high-performance liquid chromatography
In vitro propagation of Maytenus ilicifolia (Celastraceae) as potential source for antitumoral and antioxidant quinomethide triterpenes production. A rapid quantitative method for their analysis by reverse-phase high-performance liquid chromatography
title_full_unstemmed In vitro propagation of Maytenus ilicifolia (Celastraceae) as potential source for antitumoral and antioxidant quinomethide triterpenes production. A rapid quantitative method for their analysis by reverse-phase high-performance liquid chromatography
In vitro propagation of Maytenus ilicifolia (Celastraceae) as potential source for antitumoral and antioxidant quinomethide triterpenes production. A rapid quantitative method for their analysis by reverse-phase high-performance liquid chromatography
title_sort In vitro propagation of Maytenus ilicifolia (Celastraceae) as potential source for antitumoral and antioxidant quinomethide triterpenes production. A rapid quantitative method for their analysis by reverse-phase high-performance liquid chromatography
author Buffa Filho, Waldemar [UNESP]
author_facet Buffa Filho, Waldemar [UNESP]
Buffa Filho, Waldemar [UNESP]
Bolzani, Vanderlan da Silva [UNESP]
Furlan, Maysa [UNESP]
Vaz Pereira, Sarazete Izidia
Pereira, Ana Maria Soares
Françab, Suzelei Castro
Bolzani, Vanderlan da Silva [UNESP]
Furlan, Maysa [UNESP]
Vaz Pereira, Sarazete Izidia
Pereira, Ana Maria Soares
Françab, Suzelei Castro
author_role author
author2 Bolzani, Vanderlan da Silva [UNESP]
Furlan, Maysa [UNESP]
Vaz Pereira, Sarazete Izidia
Pereira, Ana Maria Soares
Françab, Suzelei Castro
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
UNAERP
dc.contributor.author.fl_str_mv Buffa Filho, Waldemar [UNESP]
Bolzani, Vanderlan da Silva [UNESP]
Furlan, Maysa [UNESP]
Vaz Pereira, Sarazete Izidia
Pereira, Ana Maria Soares
Françab, Suzelei Castro
dc.subject.por.fl_str_mv in vitro system, Celastraceae
Maytenus ilicifolia
Quantitative determination
Quinonemethide triterpenes, Reverse-phase high-performance liquid chromatography
22beta hydroxymaytenin
antineoplastic agent
antioxidant
maytenin
maytenus ilicifolia extract
plant extract
quinone derivative
triterpene derivative
unclassified drug
bioaccumulation
Celastraceae
cell culture
drug potency
drug synthesis
explant
humidity
in vitro study
Maytenus
photosynthesis
plant cell
plant leaf
quantitative analysis
reliability
reversed phase high performance liquid chromatography
standard
technique
temperature dependence
topic in vitro system, Celastraceae
Maytenus ilicifolia
Quantitative determination
Quinonemethide triterpenes, Reverse-phase high-performance liquid chromatography
22beta hydroxymaytenin
antineoplastic agent
antioxidant
maytenin
maytenus ilicifolia extract
plant extract
quinone derivative
triterpene derivative
unclassified drug
bioaccumulation
Celastraceae
cell culture
drug potency
drug synthesis
explant
humidity
in vitro study
Maytenus
photosynthesis
plant cell
plant leaf
quantitative analysis
reliability
reversed phase high performance liquid chromatography
standard
technique
temperature dependence
description Cell culture of Maytenus ilicifolia were established in order to produce and to quantify the antitumoral and antioxidant quinonemethide triterpenes. In vitro calli were induced from leaf explants of native plants and cultured in semi-solid medium under controlled conditions of humidity, temperature and photoperiod. The quinonemethide triterpenes showed maximum accumulation in the logarithmic phase growth of the cell culture. A rapid, sensitive and reliable reverse-phase HPLC method was used for quantitative determination of the antitumoral and antioxidant quinonemethide triterpenes, 22β-hydroxymaytenin and maytenin in callus of Maytenus ilicifolia. Well resolved peaks with good detection response and linearity in the range 1.0 - 100 μg/mL were obtained. This quantitative work was performed by an external standard method.
publishDate 2004
dc.date.none.fl_str_mv 2004-09-19
2014-05-27T11:21:09Z
2014-05-27T11:21:09Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.arkat-usa.org/get-file/19210/
http://dx.doi.org/10.3998/ark.5550190.0005.617
Arkivoc, v. 2004, n. 6, p. 137-146, 2004.
1424-6376
http://hdl.handle.net/11449/67879
10.3998/ark.5550190.0005.617
WOS:000224153100017
2-s2.0-5444226445
WOS000224153100017.pdf
1308042794786872
url http://www.arkat-usa.org/get-file/19210/
http://dx.doi.org/10.3998/ark.5550190.0005.617
http://hdl.handle.net/11449/67879
identifier_str_mv Arkivoc, v. 2004, n. 6, p. 137-146, 2004.
1424-6376
10.3998/ark.5550190.0005.617
WOS:000224153100017
2-s2.0-5444226445
WOS000224153100017.pdf
1308042794786872
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Arkivoc
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 137-146
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
_version_ 1822219166992564224
dc.identifier.doi.none.fl_str_mv 10.3998/ark.5550190.0005.617

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