Diazinon impairs bioenergetics and induces membrane permeability transition on mitochondria isolated from rat liver

Detalhes bibliográficos
Autor(a) principal: Miranda, Camila Araújo [UNESP]
Data de Publicação: 2020
Outros Autores: Guimarães, Anilda Rufino de Jesus Santos [UNESP], Bizerra, Paulo Francisco Veiga [UNESP], Mingatto, Fábio Erminio [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1080/15287394.2020.1805078
http://hdl.handle.net/11449/202023
Resumo: Diazinon (DZN) is a broad-spectrum insecticide extensively used to control pests in crops and animals. Several investigators demonstrated that DZN produced tissue toxicity especially to the liver. In addition, the mitochondrion was implicated in DZN-induced toxicity, but the precise role of this organelle remains to be determined. The aim of this study was thus to examine the effects of DZN (50 to 150 μM) on the bioenergetics and mitochondrial permeability transition (MPT) associated processes in isolated rat liver mitochondria. DZN inhibited state-3 respiration in mitochondria energized with glutamate plus malate, substrates of complex I, and succinate, substrate of complex II of the respiratory chain and decreased the mitochondrial membrane potential resulting in inhibition of ATP synthesis. MPT was estimated by the extent of mitochondrial swelling, in the presence of 10 µM Ca2+. DZN elicited MPT in a concentration-dependent manner, via a mechanism sensitive to cyclosporine A, EGTA, ruthenium red and N-ethylmaleimide, which was associated with mitochondrial Ca2+ efflux and cytochrome c release. DZN did not result in hydrogen peroxide accumulation or glutathione oxidation, but this insecticide oxidized endogenous NAD(P)H and protein thiol groups. Data suggest the involvement of mitochondria, via apoptosis, in the hepatic cytotoxicity attributed to DZN.
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spelling Diazinon impairs bioenergetics and induces membrane permeability transition on mitochondria isolated from rat liverDiazinonlivermitochondrial membrane permeability transitionoxidative phosphorylationtoxicityDiazinon (DZN) is a broad-spectrum insecticide extensively used to control pests in crops and animals. Several investigators demonstrated that DZN produced tissue toxicity especially to the liver. In addition, the mitochondrion was implicated in DZN-induced toxicity, but the precise role of this organelle remains to be determined. The aim of this study was thus to examine the effects of DZN (50 to 150 μM) on the bioenergetics and mitochondrial permeability transition (MPT) associated processes in isolated rat liver mitochondria. DZN inhibited state-3 respiration in mitochondria energized with glutamate plus malate, substrates of complex I, and succinate, substrate of complex II of the respiratory chain and decreased the mitochondrial membrane potential resulting in inhibition of ATP synthesis. MPT was estimated by the extent of mitochondrial swelling, in the presence of 10 µM Ca2+. DZN elicited MPT in a concentration-dependent manner, via a mechanism sensitive to cyclosporine A, EGTA, ruthenium red and N-ethylmaleimide, which was associated with mitochondrial Ca2+ efflux and cytochrome c release. DZN did not result in hydrogen peroxide accumulation or glutathione oxidation, but this insecticide oxidized endogenous NAD(P)H and protein thiol groups. Data suggest the involvement of mitochondria, via apoptosis, in the hepatic cytotoxicity attributed to DZN.Department of Animal Production College of Agricultural and Technological Sciences São Paulo State University (UnespDepartment of Animal Production College of Agricultural and Technological Sciences São Paulo State University (UnespUniversidade Estadual Paulista (Unesp)Miranda, Camila Araújo [UNESP]Guimarães, Anilda Rufino de Jesus Santos [UNESP]Bizerra, Paulo Francisco Veiga [UNESP]Mingatto, Fábio Erminio [UNESP]2020-12-12T02:47:51Z2020-12-12T02:47:51Z2020-09-16info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article616-629http://dx.doi.org/10.1080/15287394.2020.1805078Journal of Toxicology and Environmental Health - Part A: Current Issues, v. 83, n. 17-18, p. 616-629, 2020.1087-26201528-7394http://hdl.handle.net/11449/20202310.1080/15287394.2020.18050782-s2.0-85089456708Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Toxicology and Environmental Health - Part A: Current Issuesinfo:eu-repo/semantics/openAccess2024-05-07T13:47:36Zoai:repositorio.unesp.br:11449/202023Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:16:37.021160Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Diazinon impairs bioenergetics and induces membrane permeability transition on mitochondria isolated from rat liver
title Diazinon impairs bioenergetics and induces membrane permeability transition on mitochondria isolated from rat liver
spellingShingle Diazinon impairs bioenergetics and induces membrane permeability transition on mitochondria isolated from rat liver
Miranda, Camila Araújo [UNESP]
Diazinon
liver
mitochondrial membrane permeability transition
oxidative phosphorylation
toxicity
title_short Diazinon impairs bioenergetics and induces membrane permeability transition on mitochondria isolated from rat liver
title_full Diazinon impairs bioenergetics and induces membrane permeability transition on mitochondria isolated from rat liver
title_fullStr Diazinon impairs bioenergetics and induces membrane permeability transition on mitochondria isolated from rat liver
title_full_unstemmed Diazinon impairs bioenergetics and induces membrane permeability transition on mitochondria isolated from rat liver
title_sort Diazinon impairs bioenergetics and induces membrane permeability transition on mitochondria isolated from rat liver
author Miranda, Camila Araújo [UNESP]
author_facet Miranda, Camila Araújo [UNESP]
Guimarães, Anilda Rufino de Jesus Santos [UNESP]
Bizerra, Paulo Francisco Veiga [UNESP]
Mingatto, Fábio Erminio [UNESP]
author_role author
author2 Guimarães, Anilda Rufino de Jesus Santos [UNESP]
Bizerra, Paulo Francisco Veiga [UNESP]
Mingatto, Fábio Erminio [UNESP]
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Miranda, Camila Araújo [UNESP]
Guimarães, Anilda Rufino de Jesus Santos [UNESP]
Bizerra, Paulo Francisco Veiga [UNESP]
Mingatto, Fábio Erminio [UNESP]
dc.subject.por.fl_str_mv Diazinon
liver
mitochondrial membrane permeability transition
oxidative phosphorylation
toxicity
topic Diazinon
liver
mitochondrial membrane permeability transition
oxidative phosphorylation
toxicity
description Diazinon (DZN) is a broad-spectrum insecticide extensively used to control pests in crops and animals. Several investigators demonstrated that DZN produced tissue toxicity especially to the liver. In addition, the mitochondrion was implicated in DZN-induced toxicity, but the precise role of this organelle remains to be determined. The aim of this study was thus to examine the effects of DZN (50 to 150 μM) on the bioenergetics and mitochondrial permeability transition (MPT) associated processes in isolated rat liver mitochondria. DZN inhibited state-3 respiration in mitochondria energized with glutamate plus malate, substrates of complex I, and succinate, substrate of complex II of the respiratory chain and decreased the mitochondrial membrane potential resulting in inhibition of ATP synthesis. MPT was estimated by the extent of mitochondrial swelling, in the presence of 10 µM Ca2+. DZN elicited MPT in a concentration-dependent manner, via a mechanism sensitive to cyclosporine A, EGTA, ruthenium red and N-ethylmaleimide, which was associated with mitochondrial Ca2+ efflux and cytochrome c release. DZN did not result in hydrogen peroxide accumulation or glutathione oxidation, but this insecticide oxidized endogenous NAD(P)H and protein thiol groups. Data suggest the involvement of mitochondria, via apoptosis, in the hepatic cytotoxicity attributed to DZN.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-12T02:47:51Z
2020-12-12T02:47:51Z
2020-09-16
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1080/15287394.2020.1805078
Journal of Toxicology and Environmental Health - Part A: Current Issues, v. 83, n. 17-18, p. 616-629, 2020.
1087-2620
1528-7394
http://hdl.handle.net/11449/202023
10.1080/15287394.2020.1805078
2-s2.0-85089456708
url http://dx.doi.org/10.1080/15287394.2020.1805078
http://hdl.handle.net/11449/202023
identifier_str_mv Journal of Toxicology and Environmental Health - Part A: Current Issues, v. 83, n. 17-18, p. 616-629, 2020.
1087-2620
1528-7394
10.1080/15287394.2020.1805078
2-s2.0-85089456708
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Toxicology and Environmental Health - Part A: Current Issues
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 616-629
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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