Bioactivity of the Protein Hydrolysates Obtained from the Most Abundant Crustacean Bycatch
Autor(a) principal: | |
---|---|
Data de Publicação: | 2021 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s10126-021-10072-1 http://hdl.handle.net/11449/229825 |
Resumo: | The animals from bycatch of the shrimp fisheries can be a source of natural products and bioactive compounds. Thus, the present study aimed to evaluate the bioactivity of protein hydrolysates prepared from the two most abundant crabs from the bycatch of shrimp fisheries in Brazil (Callinectes ornatus and Hepatus pudibundus). Samples of C. ornatus and H. pudibundus were collected in the region of Ubatuba, State of São Paulo, Brazil. Muscles with small pieces of exoskeleton of both species were hydrolyzed using two enzymes, Alcalase 2.4 L® or Protamex®. The in vitro antioxidant capacity was analyzed used three methods: DPPH, sulfhydryl groups, and peroxyl radicals. Additionally, the cytotoxicity of the hydrolysates was investigated using pre-osteoblasts cells. The results showed that the degree of hydrolysis (DH) of H. pudibundus was superior to DH of C. ornatus using both enzymes and was higher when using the enzyme Alcalase 2.4 L® (32.0% ± 1.9). The analysis suggested that the hydrolysates have antioxidant activity. Besides that, no cytotoxic effect was observed on cell viability. Thus, protein hydrolysates of C. ornatus and H. pudibundus have bioactivity, which add value to these bycatch species and suggests their potential use as nutraceutical ingredient in the food industry. Graphic Abstract: [Figure not available: see fulltext.] |
id |
UNSP_5b375536e0a200fa1ba77ae2a2b379a9 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/229825 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Bioactivity of the Protein Hydrolysates Obtained from the Most Abundant Crustacean BycatchAntioxidant activityCell viabilityDPPHEnzymatic hydrolysisThe animals from bycatch of the shrimp fisheries can be a source of natural products and bioactive compounds. Thus, the present study aimed to evaluate the bioactivity of protein hydrolysates prepared from the two most abundant crabs from the bycatch of shrimp fisheries in Brazil (Callinectes ornatus and Hepatus pudibundus). Samples of C. ornatus and H. pudibundus were collected in the region of Ubatuba, State of São Paulo, Brazil. Muscles with small pieces of exoskeleton of both species were hydrolyzed using two enzymes, Alcalase 2.4 L® or Protamex®. The in vitro antioxidant capacity was analyzed used three methods: DPPH, sulfhydryl groups, and peroxyl radicals. Additionally, the cytotoxicity of the hydrolysates was investigated using pre-osteoblasts cells. The results showed that the degree of hydrolysis (DH) of H. pudibundus was superior to DH of C. ornatus using both enzymes and was higher when using the enzyme Alcalase 2.4 L® (32.0% ± 1.9). The analysis suggested that the hydrolysates have antioxidant activity. Besides that, no cytotoxic effect was observed on cell viability. Thus, protein hydrolysates of C. ornatus and H. pudibundus have bioactivity, which add value to these bycatch species and suggests their potential use as nutraceutical ingredient in the food industry. Graphic Abstract: [Figure not available: see fulltext.]Aquaculture Center São Paulo State University (UNESP), Campus Jaboticabal, Via de Acesso Prof. Paulo Donato Castellane, s/n 14884-900Marine Station of Aquaculture Oceanography Institute Federal University of Rio Grande (FURG) Rio Grande, Aquaculture postgraduateInstitute of Biological Sciences (ICB) Federal University of Rio Grande (FURG)Department of Chemical and Biological Sciences Bioscience Institute São Paulo State University (UNESP), Campus BotucatuAquaculture Center São Paulo State University (UNESP), Campus Jaboticabal, Via de Acesso Prof. Paulo Donato Castellane, s/n 14884-900Department of Chemical and Biological Sciences Bioscience Institute São Paulo State University (UNESP), Campus BotucatuUniversidade Estadual Paulista (UNESP)Federal University of Rio Grande (FURG) Rio GrandeFederal University of Rio Grande (FURG)Camargo, Tavani R. [UNESP]Mantoan, Paulo [UNESP]Ramos, PatríciaMonserrat, José M.Prentice, CarlosFernandes, Célio C. [UNESP]Zambuzzi, Willian F. [UNESP]Valenti, Wagner C. [UNESP]2022-04-29T08:36:09Z2022-04-29T08:36:09Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1007/s10126-021-10072-1Marine Biotechnology.1436-22361436-2228http://hdl.handle.net/11449/22982510.1007/s10126-021-10072-12-s2.0-85118378797Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMarine Biotechnologyinfo:eu-repo/semantics/openAccess2024-04-09T15:43:35Zoai:repositorio.unesp.br:11449/229825Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-04-09T15:43:35Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Bioactivity of the Protein Hydrolysates Obtained from the Most Abundant Crustacean Bycatch |
title |
Bioactivity of the Protein Hydrolysates Obtained from the Most Abundant Crustacean Bycatch |
spellingShingle |
Bioactivity of the Protein Hydrolysates Obtained from the Most Abundant Crustacean Bycatch Camargo, Tavani R. [UNESP] Antioxidant activity Cell viability DPPH Enzymatic hydrolysis |
title_short |
Bioactivity of the Protein Hydrolysates Obtained from the Most Abundant Crustacean Bycatch |
title_full |
Bioactivity of the Protein Hydrolysates Obtained from the Most Abundant Crustacean Bycatch |
title_fullStr |
Bioactivity of the Protein Hydrolysates Obtained from the Most Abundant Crustacean Bycatch |
title_full_unstemmed |
Bioactivity of the Protein Hydrolysates Obtained from the Most Abundant Crustacean Bycatch |
title_sort |
Bioactivity of the Protein Hydrolysates Obtained from the Most Abundant Crustacean Bycatch |
author |
Camargo, Tavani R. [UNESP] |
author_facet |
Camargo, Tavani R. [UNESP] Mantoan, Paulo [UNESP] Ramos, Patrícia Monserrat, José M. Prentice, Carlos Fernandes, Célio C. [UNESP] Zambuzzi, Willian F. [UNESP] Valenti, Wagner C. [UNESP] |
author_role |
author |
author2 |
Mantoan, Paulo [UNESP] Ramos, Patrícia Monserrat, José M. Prentice, Carlos Fernandes, Célio C. [UNESP] Zambuzzi, Willian F. [UNESP] Valenti, Wagner C. [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Federal University of Rio Grande (FURG) Rio Grande Federal University of Rio Grande (FURG) |
dc.contributor.author.fl_str_mv |
Camargo, Tavani R. [UNESP] Mantoan, Paulo [UNESP] Ramos, Patrícia Monserrat, José M. Prentice, Carlos Fernandes, Célio C. [UNESP] Zambuzzi, Willian F. [UNESP] Valenti, Wagner C. [UNESP] |
dc.subject.por.fl_str_mv |
Antioxidant activity Cell viability DPPH Enzymatic hydrolysis |
topic |
Antioxidant activity Cell viability DPPH Enzymatic hydrolysis |
description |
The animals from bycatch of the shrimp fisheries can be a source of natural products and bioactive compounds. Thus, the present study aimed to evaluate the bioactivity of protein hydrolysates prepared from the two most abundant crabs from the bycatch of shrimp fisheries in Brazil (Callinectes ornatus and Hepatus pudibundus). Samples of C. ornatus and H. pudibundus were collected in the region of Ubatuba, State of São Paulo, Brazil. Muscles with small pieces of exoskeleton of both species were hydrolyzed using two enzymes, Alcalase 2.4 L® or Protamex®. The in vitro antioxidant capacity was analyzed used three methods: DPPH, sulfhydryl groups, and peroxyl radicals. Additionally, the cytotoxicity of the hydrolysates was investigated using pre-osteoblasts cells. The results showed that the degree of hydrolysis (DH) of H. pudibundus was superior to DH of C. ornatus using both enzymes and was higher when using the enzyme Alcalase 2.4 L® (32.0% ± 1.9). The analysis suggested that the hydrolysates have antioxidant activity. Besides that, no cytotoxic effect was observed on cell viability. Thus, protein hydrolysates of C. ornatus and H. pudibundus have bioactivity, which add value to these bycatch species and suggests their potential use as nutraceutical ingredient in the food industry. Graphic Abstract: [Figure not available: see fulltext.] |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-01 2022-04-29T08:36:09Z 2022-04-29T08:36:09Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s10126-021-10072-1 Marine Biotechnology. 1436-2236 1436-2228 http://hdl.handle.net/11449/229825 10.1007/s10126-021-10072-1 2-s2.0-85118378797 |
url |
http://dx.doi.org/10.1007/s10126-021-10072-1 http://hdl.handle.net/11449/229825 |
identifier_str_mv |
Marine Biotechnology. 1436-2236 1436-2228 10.1007/s10126-021-10072-1 2-s2.0-85118378797 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Marine Biotechnology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803047341042696192 |