Immunogenicity in Oreochromis niloticus vaccinated with sonicated antigens against streptococcosis

Detalhes bibliográficos
Autor(a) principal: Marcusso, Paulo Fernandes [UNESP]
Data de Publicação: 2021
Outros Autores: da Silva Claudiano, Gustavo [UNESP], Yunis-Aguinaga, Jefferson [UNESP], de Almeida Marinho-Neto, Fausto [UNESP], Eto, Silas Fernandes [UNESP], Fernandes, Dayanne Carla [UNESP], Rosolem, Mayara Caroline [UNESP], Salvador, Rogério, Engracia de Moraes, Julieta Rodini [UNESP], Ruas de Moraes, Flávio [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.fsi.2021.04.030
http://hdl.handle.net/11449/228970
Resumo: Streptococcosis causes great economic losses in intensive culture of tilapia. Vaccination is the most effective and safest way to tackle infectious diseases. Thus, this study sought the more effective and safer antigenic fraction after sonication of Streptococcus agalactiae to elaborate a vaccine against streptococcosis in Nile tilapia. For this, twenty-one days after vaccination with different fractions (soluble and insoluble) of S. agalactiae, the fish were challenged with the homologous strain (LD50). Then, samples were taken at zero, 14, 28, 60 and 90 days post-vaccination (DPV, n = 7). Blood and organs (cranial kidney, spleen and liver) were collected from vaccinated and unvaccinated fish. Finally, insoluble fraction vaccine presented the best effect, resulting in a 100% relative percent of survival (RPS) and without clinical manifestations. In view of the results, it was to evaluate the role of the insoluble fraction of the antigen in the protective immunity against streptococcosis. The results indicate that the spleen might be the main organ in the vaccine response in Nile tilapia due to the great morphological and immunological differences in vaccinated fish, evidenced by the greater of melanomacrophage centers (MMC) and IgM + lymphocytes in relation to the non-vaccinated fish. At 60 DPV, it was observed the peak of the protective immunity related to the maximum concentration of proteins, circulating leukocytes, antibody titers in the serum and tissue changes with greater expression of IgM + and MMC number in the spleen and kidney of Oreochromis niloticus. Vaccination with insoluble fraction of S. agalactiae was safe and provided effective protection against streptococcosis with maximum protective response at 60 DPV.
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spelling Immunogenicity in Oreochromis niloticus vaccinated with sonicated antigens against streptococcosisImmunityImmunopathologyImmunoprophylaxisNile tilapiaStreptococcosisVaccinationStreptococcosis causes great economic losses in intensive culture of tilapia. Vaccination is the most effective and safest way to tackle infectious diseases. Thus, this study sought the more effective and safer antigenic fraction after sonication of Streptococcus agalactiae to elaborate a vaccine against streptococcosis in Nile tilapia. For this, twenty-one days after vaccination with different fractions (soluble and insoluble) of S. agalactiae, the fish were challenged with the homologous strain (LD50). Then, samples were taken at zero, 14, 28, 60 and 90 days post-vaccination (DPV, n = 7). Blood and organs (cranial kidney, spleen and liver) were collected from vaccinated and unvaccinated fish. Finally, insoluble fraction vaccine presented the best effect, resulting in a 100% relative percent of survival (RPS) and without clinical manifestations. In view of the results, it was to evaluate the role of the insoluble fraction of the antigen in the protective immunity against streptococcosis. The results indicate that the spleen might be the main organ in the vaccine response in Nile tilapia due to the great morphological and immunological differences in vaccinated fish, evidenced by the greater of melanomacrophage centers (MMC) and IgM + lymphocytes in relation to the non-vaccinated fish. At 60 DPV, it was observed the peak of the protective immunity related to the maximum concentration of proteins, circulating leukocytes, antibody titers in the serum and tissue changes with greater expression of IgM + and MMC number in the spleen and kidney of Oreochromis niloticus. Vaccination with insoluble fraction of S. agalactiae was safe and provided effective protection against streptococcosis with maximum protective response at 60 DPV.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Canada's Michael Smith Genome Sciences CentreInstitute of Agricultural Sciences Federal University of the Jequitinhonha and Mucuri ValleysDepartment of Pathology Theriogenology and One Health Faculty of Agrarian and Veterinarian Sciences São Paulo State University (UNESP)Institute of Biodiversity and Forests Federal University of Western Pará UFOPAScientific University of the SouthSea Institute of PeruPostgraduated Program in Aquaculture/Aquaculture Center São Paulo State University (UNESP) JaboticabalDepartment of Postgraduate in Health Sciences—PROCISA Federal University of Roraima (UFRR)Institute of Chemistry São Paulo State University (Unesp)Veterinary Pathologist AutonomousState University of Northern Paraná. BandeirantesDepartment of Pathology Theriogenology and One Health Faculty of Agrarian and Veterinarian Sciences São Paulo State University (UNESP)Postgraduated Program in Aquaculture/Aquaculture Center São Paulo State University (UNESP) JaboticabalInstitute of Chemistry São Paulo State University (Unesp)Federal University of the Jequitinhonha and Mucuri ValleysUniversidade Estadual Paulista (UNESP)UFOPAScientific University of the SouthSea Institute of PeruFederal University of Roraima (UFRR)Veterinary Pathologist AutonomousState University of Northern Paraná. BandeirantesMarcusso, Paulo Fernandes [UNESP]da Silva Claudiano, Gustavo [UNESP]Yunis-Aguinaga, Jefferson [UNESP]de Almeida Marinho-Neto, Fausto [UNESP]Eto, Silas Fernandes [UNESP]Fernandes, Dayanne Carla [UNESP]Rosolem, Mayara Caroline [UNESP]Salvador, RogérioEngracia de Moraes, Julieta Rodini [UNESP]Ruas de Moraes, Flávio [UNESP]2022-04-29T08:29:37Z2022-04-29T08:29:37Z2021-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article134-141http://dx.doi.org/10.1016/j.fsi.2021.04.030Fish and Shellfish Immunology, v. 115, p. 134-141.1095-99471050-4648http://hdl.handle.net/11449/22897010.1016/j.fsi.2021.04.0302-s2.0-85107932148Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFish and Shellfish Immunologyinfo:eu-repo/semantics/openAccess2024-04-09T15:43:25Zoai:repositorio.unesp.br:11449/228970Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-04-09T15:43:25Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Immunogenicity in Oreochromis niloticus vaccinated with sonicated antigens against streptococcosis
title Immunogenicity in Oreochromis niloticus vaccinated with sonicated antigens against streptococcosis
spellingShingle Immunogenicity in Oreochromis niloticus vaccinated with sonicated antigens against streptococcosis
Marcusso, Paulo Fernandes [UNESP]
Immunity
Immunopathology
Immunoprophylaxis
Nile tilapia
Streptococcosis
Vaccination
title_short Immunogenicity in Oreochromis niloticus vaccinated with sonicated antigens against streptococcosis
title_full Immunogenicity in Oreochromis niloticus vaccinated with sonicated antigens against streptococcosis
title_fullStr Immunogenicity in Oreochromis niloticus vaccinated with sonicated antigens against streptococcosis
title_full_unstemmed Immunogenicity in Oreochromis niloticus vaccinated with sonicated antigens against streptococcosis
title_sort Immunogenicity in Oreochromis niloticus vaccinated with sonicated antigens against streptococcosis
author Marcusso, Paulo Fernandes [UNESP]
author_facet Marcusso, Paulo Fernandes [UNESP]
da Silva Claudiano, Gustavo [UNESP]
Yunis-Aguinaga, Jefferson [UNESP]
de Almeida Marinho-Neto, Fausto [UNESP]
Eto, Silas Fernandes [UNESP]
Fernandes, Dayanne Carla [UNESP]
Rosolem, Mayara Caroline [UNESP]
Salvador, Rogério
Engracia de Moraes, Julieta Rodini [UNESP]
Ruas de Moraes, Flávio [UNESP]
author_role author
author2 da Silva Claudiano, Gustavo [UNESP]
Yunis-Aguinaga, Jefferson [UNESP]
de Almeida Marinho-Neto, Fausto [UNESP]
Eto, Silas Fernandes [UNESP]
Fernandes, Dayanne Carla [UNESP]
Rosolem, Mayara Caroline [UNESP]
Salvador, Rogério
Engracia de Moraes, Julieta Rodini [UNESP]
Ruas de Moraes, Flávio [UNESP]
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Federal University of the Jequitinhonha and Mucuri Valleys
Universidade Estadual Paulista (UNESP)
UFOPA
Scientific University of the South
Sea Institute of Peru
Federal University of Roraima (UFRR)
Veterinary Pathologist Autonomous
State University of Northern Paraná. Bandeirantes
dc.contributor.author.fl_str_mv Marcusso, Paulo Fernandes [UNESP]
da Silva Claudiano, Gustavo [UNESP]
Yunis-Aguinaga, Jefferson [UNESP]
de Almeida Marinho-Neto, Fausto [UNESP]
Eto, Silas Fernandes [UNESP]
Fernandes, Dayanne Carla [UNESP]
Rosolem, Mayara Caroline [UNESP]
Salvador, Rogério
Engracia de Moraes, Julieta Rodini [UNESP]
Ruas de Moraes, Flávio [UNESP]
dc.subject.por.fl_str_mv Immunity
Immunopathology
Immunoprophylaxis
Nile tilapia
Streptococcosis
Vaccination
topic Immunity
Immunopathology
Immunoprophylaxis
Nile tilapia
Streptococcosis
Vaccination
description Streptococcosis causes great economic losses in intensive culture of tilapia. Vaccination is the most effective and safest way to tackle infectious diseases. Thus, this study sought the more effective and safer antigenic fraction after sonication of Streptococcus agalactiae to elaborate a vaccine against streptococcosis in Nile tilapia. For this, twenty-one days after vaccination with different fractions (soluble and insoluble) of S. agalactiae, the fish were challenged with the homologous strain (LD50). Then, samples were taken at zero, 14, 28, 60 and 90 days post-vaccination (DPV, n = 7). Blood and organs (cranial kidney, spleen and liver) were collected from vaccinated and unvaccinated fish. Finally, insoluble fraction vaccine presented the best effect, resulting in a 100% relative percent of survival (RPS) and without clinical manifestations. In view of the results, it was to evaluate the role of the insoluble fraction of the antigen in the protective immunity against streptococcosis. The results indicate that the spleen might be the main organ in the vaccine response in Nile tilapia due to the great morphological and immunological differences in vaccinated fish, evidenced by the greater of melanomacrophage centers (MMC) and IgM + lymphocytes in relation to the non-vaccinated fish. At 60 DPV, it was observed the peak of the protective immunity related to the maximum concentration of proteins, circulating leukocytes, antibody titers in the serum and tissue changes with greater expression of IgM + and MMC number in the spleen and kidney of Oreochromis niloticus. Vaccination with insoluble fraction of S. agalactiae was safe and provided effective protection against streptococcosis with maximum protective response at 60 DPV.
publishDate 2021
dc.date.none.fl_str_mv 2021-08-01
2022-04-29T08:29:37Z
2022-04-29T08:29:37Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.fsi.2021.04.030
Fish and Shellfish Immunology, v. 115, p. 134-141.
1095-9947
1050-4648
http://hdl.handle.net/11449/228970
10.1016/j.fsi.2021.04.030
2-s2.0-85107932148
url http://dx.doi.org/10.1016/j.fsi.2021.04.030
http://hdl.handle.net/11449/228970
identifier_str_mv Fish and Shellfish Immunology, v. 115, p. 134-141.
1095-9947
1050-4648
10.1016/j.fsi.2021.04.030
2-s2.0-85107932148
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Fish and Shellfish Immunology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 134-141
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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