Immunogenicity in Oreochromis niloticus vaccinated with sonicated antigens against streptococcosis
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.fsi.2021.04.030 http://hdl.handle.net/11449/228970 |
Resumo: | Streptococcosis causes great economic losses in intensive culture of tilapia. Vaccination is the most effective and safest way to tackle infectious diseases. Thus, this study sought the more effective and safer antigenic fraction after sonication of Streptococcus agalactiae to elaborate a vaccine against streptococcosis in Nile tilapia. For this, twenty-one days after vaccination with different fractions (soluble and insoluble) of S. agalactiae, the fish were challenged with the homologous strain (LD50). Then, samples were taken at zero, 14, 28, 60 and 90 days post-vaccination (DPV, n = 7). Blood and organs (cranial kidney, spleen and liver) were collected from vaccinated and unvaccinated fish. Finally, insoluble fraction vaccine presented the best effect, resulting in a 100% relative percent of survival (RPS) and without clinical manifestations. In view of the results, it was to evaluate the role of the insoluble fraction of the antigen in the protective immunity against streptococcosis. The results indicate that the spleen might be the main organ in the vaccine response in Nile tilapia due to the great morphological and immunological differences in vaccinated fish, evidenced by the greater of melanomacrophage centers (MMC) and IgM + lymphocytes in relation to the non-vaccinated fish. At 60 DPV, it was observed the peak of the protective immunity related to the maximum concentration of proteins, circulating leukocytes, antibody titers in the serum and tissue changes with greater expression of IgM + and MMC number in the spleen and kidney of Oreochromis niloticus. Vaccination with insoluble fraction of S. agalactiae was safe and provided effective protection against streptococcosis with maximum protective response at 60 DPV. |
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Immunogenicity in Oreochromis niloticus vaccinated with sonicated antigens against streptococcosisImmunityImmunopathologyImmunoprophylaxisNile tilapiaStreptococcosisVaccinationStreptococcosis causes great economic losses in intensive culture of tilapia. Vaccination is the most effective and safest way to tackle infectious diseases. Thus, this study sought the more effective and safer antigenic fraction after sonication of Streptococcus agalactiae to elaborate a vaccine against streptococcosis in Nile tilapia. For this, twenty-one days after vaccination with different fractions (soluble and insoluble) of S. agalactiae, the fish were challenged with the homologous strain (LD50). Then, samples were taken at zero, 14, 28, 60 and 90 days post-vaccination (DPV, n = 7). Blood and organs (cranial kidney, spleen and liver) were collected from vaccinated and unvaccinated fish. Finally, insoluble fraction vaccine presented the best effect, resulting in a 100% relative percent of survival (RPS) and without clinical manifestations. In view of the results, it was to evaluate the role of the insoluble fraction of the antigen in the protective immunity against streptococcosis. The results indicate that the spleen might be the main organ in the vaccine response in Nile tilapia due to the great morphological and immunological differences in vaccinated fish, evidenced by the greater of melanomacrophage centers (MMC) and IgM + lymphocytes in relation to the non-vaccinated fish. At 60 DPV, it was observed the peak of the protective immunity related to the maximum concentration of proteins, circulating leukocytes, antibody titers in the serum and tissue changes with greater expression of IgM + and MMC number in the spleen and kidney of Oreochromis niloticus. Vaccination with insoluble fraction of S. agalactiae was safe and provided effective protection against streptococcosis with maximum protective response at 60 DPV.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Canada's Michael Smith Genome Sciences CentreInstitute of Agricultural Sciences Federal University of the Jequitinhonha and Mucuri ValleysDepartment of Pathology Theriogenology and One Health Faculty of Agrarian and Veterinarian Sciences São Paulo State University (UNESP)Institute of Biodiversity and Forests Federal University of Western Pará UFOPAScientific University of the SouthSea Institute of PeruPostgraduated Program in Aquaculture/Aquaculture Center São Paulo State University (UNESP) JaboticabalDepartment of Postgraduate in Health Sciences—PROCISA Federal University of Roraima (UFRR)Institute of Chemistry São Paulo State University (Unesp)Veterinary Pathologist AutonomousState University of Northern Paraná. BandeirantesDepartment of Pathology Theriogenology and One Health Faculty of Agrarian and Veterinarian Sciences São Paulo State University (UNESP)Postgraduated Program in Aquaculture/Aquaculture Center São Paulo State University (UNESP) JaboticabalInstitute of Chemistry São Paulo State University (Unesp)Federal University of the Jequitinhonha and Mucuri ValleysUniversidade Estadual Paulista (UNESP)UFOPAScientific University of the SouthSea Institute of PeruFederal University of Roraima (UFRR)Veterinary Pathologist AutonomousState University of Northern Paraná. BandeirantesMarcusso, Paulo Fernandes [UNESP]da Silva Claudiano, Gustavo [UNESP]Yunis-Aguinaga, Jefferson [UNESP]de Almeida Marinho-Neto, Fausto [UNESP]Eto, Silas Fernandes [UNESP]Fernandes, Dayanne Carla [UNESP]Rosolem, Mayara Caroline [UNESP]Salvador, RogérioEngracia de Moraes, Julieta Rodini [UNESP]Ruas de Moraes, Flávio [UNESP]2022-04-29T08:29:37Z2022-04-29T08:29:37Z2021-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article134-141http://dx.doi.org/10.1016/j.fsi.2021.04.030Fish and Shellfish Immunology, v. 115, p. 134-141.1095-99471050-4648http://hdl.handle.net/11449/22897010.1016/j.fsi.2021.04.0302-s2.0-85107932148Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFish and Shellfish Immunologyinfo:eu-repo/semantics/openAccess2024-04-09T15:43:25Zoai:repositorio.unesp.br:11449/228970Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-04-09T15:43:25Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Immunogenicity in Oreochromis niloticus vaccinated with sonicated antigens against streptococcosis |
title |
Immunogenicity in Oreochromis niloticus vaccinated with sonicated antigens against streptococcosis |
spellingShingle |
Immunogenicity in Oreochromis niloticus vaccinated with sonicated antigens against streptococcosis Marcusso, Paulo Fernandes [UNESP] Immunity Immunopathology Immunoprophylaxis Nile tilapia Streptococcosis Vaccination |
title_short |
Immunogenicity in Oreochromis niloticus vaccinated with sonicated antigens against streptococcosis |
title_full |
Immunogenicity in Oreochromis niloticus vaccinated with sonicated antigens against streptococcosis |
title_fullStr |
Immunogenicity in Oreochromis niloticus vaccinated with sonicated antigens against streptococcosis |
title_full_unstemmed |
Immunogenicity in Oreochromis niloticus vaccinated with sonicated antigens against streptococcosis |
title_sort |
Immunogenicity in Oreochromis niloticus vaccinated with sonicated antigens against streptococcosis |
author |
Marcusso, Paulo Fernandes [UNESP] |
author_facet |
Marcusso, Paulo Fernandes [UNESP] da Silva Claudiano, Gustavo [UNESP] Yunis-Aguinaga, Jefferson [UNESP] de Almeida Marinho-Neto, Fausto [UNESP] Eto, Silas Fernandes [UNESP] Fernandes, Dayanne Carla [UNESP] Rosolem, Mayara Caroline [UNESP] Salvador, Rogério Engracia de Moraes, Julieta Rodini [UNESP] Ruas de Moraes, Flávio [UNESP] |
author_role |
author |
author2 |
da Silva Claudiano, Gustavo [UNESP] Yunis-Aguinaga, Jefferson [UNESP] de Almeida Marinho-Neto, Fausto [UNESP] Eto, Silas Fernandes [UNESP] Fernandes, Dayanne Carla [UNESP] Rosolem, Mayara Caroline [UNESP] Salvador, Rogério Engracia de Moraes, Julieta Rodini [UNESP] Ruas de Moraes, Flávio [UNESP] |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Federal University of the Jequitinhonha and Mucuri Valleys Universidade Estadual Paulista (UNESP) UFOPA Scientific University of the South Sea Institute of Peru Federal University of Roraima (UFRR) Veterinary Pathologist Autonomous State University of Northern Paraná. Bandeirantes |
dc.contributor.author.fl_str_mv |
Marcusso, Paulo Fernandes [UNESP] da Silva Claudiano, Gustavo [UNESP] Yunis-Aguinaga, Jefferson [UNESP] de Almeida Marinho-Neto, Fausto [UNESP] Eto, Silas Fernandes [UNESP] Fernandes, Dayanne Carla [UNESP] Rosolem, Mayara Caroline [UNESP] Salvador, Rogério Engracia de Moraes, Julieta Rodini [UNESP] Ruas de Moraes, Flávio [UNESP] |
dc.subject.por.fl_str_mv |
Immunity Immunopathology Immunoprophylaxis Nile tilapia Streptococcosis Vaccination |
topic |
Immunity Immunopathology Immunoprophylaxis Nile tilapia Streptococcosis Vaccination |
description |
Streptococcosis causes great economic losses in intensive culture of tilapia. Vaccination is the most effective and safest way to tackle infectious diseases. Thus, this study sought the more effective and safer antigenic fraction after sonication of Streptococcus agalactiae to elaborate a vaccine against streptococcosis in Nile tilapia. For this, twenty-one days after vaccination with different fractions (soluble and insoluble) of S. agalactiae, the fish were challenged with the homologous strain (LD50). Then, samples were taken at zero, 14, 28, 60 and 90 days post-vaccination (DPV, n = 7). Blood and organs (cranial kidney, spleen and liver) were collected from vaccinated and unvaccinated fish. Finally, insoluble fraction vaccine presented the best effect, resulting in a 100% relative percent of survival (RPS) and without clinical manifestations. In view of the results, it was to evaluate the role of the insoluble fraction of the antigen in the protective immunity against streptococcosis. The results indicate that the spleen might be the main organ in the vaccine response in Nile tilapia due to the great morphological and immunological differences in vaccinated fish, evidenced by the greater of melanomacrophage centers (MMC) and IgM + lymphocytes in relation to the non-vaccinated fish. At 60 DPV, it was observed the peak of the protective immunity related to the maximum concentration of proteins, circulating leukocytes, antibody titers in the serum and tissue changes with greater expression of IgM + and MMC number in the spleen and kidney of Oreochromis niloticus. Vaccination with insoluble fraction of S. agalactiae was safe and provided effective protection against streptococcosis with maximum protective response at 60 DPV. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-08-01 2022-04-29T08:29:37Z 2022-04-29T08:29:37Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.fsi.2021.04.030 Fish and Shellfish Immunology, v. 115, p. 134-141. 1095-9947 1050-4648 http://hdl.handle.net/11449/228970 10.1016/j.fsi.2021.04.030 2-s2.0-85107932148 |
url |
http://dx.doi.org/10.1016/j.fsi.2021.04.030 http://hdl.handle.net/11449/228970 |
identifier_str_mv |
Fish and Shellfish Immunology, v. 115, p. 134-141. 1095-9947 1050-4648 10.1016/j.fsi.2021.04.030 2-s2.0-85107932148 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Fish and Shellfish Immunology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
134-141 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965565793599488 |