Influência do exercício físico e da N-acetilcisteína no remodelamento cardíaco e estresse oxidativo de ratos com sobrecarga pressórica crônica

Detalhes bibliográficos
Autor(a) principal: Abreu Reyes, David Rafael [UNESP]
Data de Publicação: 2017
Tipo de documento: Tese
Idioma: por
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://hdl.handle.net/11449/151905
Resumo: Objective. To evaluate the effects of N-acetylcysteine (NAC) on cardiac remodeling in rats during transition from compensated left ventricular (LV) hypertrophy to heart failure. Methods and Results. Four months after inducing aortic stenosis (AS), Wistar rats were assigned into three groups: Sham, AS, and AS treated with NAC (AS-NAC for eight weeks). NAC restored myocardial total glutathione (Sham 20.8±3.00; AS 12.6±2.92; AS-NAC 17.6±2.45 nmol/g tissue; p<0.05 AS vs Sham and AS-NAC). Malondialdehyde serum concentration was lower in AS-NAC and myocardial lipid hydroperoxide was higher in AS (Sham 199±48.1; AS 301±36.0; AS-NAC 181±41.3 nmol/g tissue). Glutathione peroxidase activity was lower in AS than Sham. Echocardiogram showed LV concentric hypertrophy and dysfunction before and after treatment. Tissue Doppler imaging (TDI) of mitral annulus systolic velocity and isovolumetric relaxation time were lower in AS than Sham; in AS-NAC these parameters were between those in Sham and AS and did not differ from either group. NAC reduced p-ERK and p-JNK protein expression, attenuated myocyte hypertrophy and myocardial fibrosis, and decreased lung congestion frequency. Conclusion. N-acetylcysteine restores myocardial total glutathione, reduces oxidative stress, improves MAPK signaling, and attenuates myocyte hypertrophy, myocardial fibrosis, and LV dysfunction in aortic stenosis rats.
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spelling Influência do exercício físico e da N-acetilcisteína no remodelamento cardíaco e estresse oxidativo de ratos com sobrecarga pressórica crônicaN-acetylcysteine influence on oxidative stress and cardiac remodeling in rats during transition from compensated left ventricular hypertrophy to heart failureExercício físicoInsuficiência cardíacaN-AcetilcisteínaVentrículo izquierdoObjective. To evaluate the effects of N-acetylcysteine (NAC) on cardiac remodeling in rats during transition from compensated left ventricular (LV) hypertrophy to heart failure. Methods and Results. Four months after inducing aortic stenosis (AS), Wistar rats were assigned into three groups: Sham, AS, and AS treated with NAC (AS-NAC for eight weeks). NAC restored myocardial total glutathione (Sham 20.8±3.00; AS 12.6±2.92; AS-NAC 17.6±2.45 nmol/g tissue; p<0.05 AS vs Sham and AS-NAC). Malondialdehyde serum concentration was lower in AS-NAC and myocardial lipid hydroperoxide was higher in AS (Sham 199±48.1; AS 301±36.0; AS-NAC 181±41.3 nmol/g tissue). Glutathione peroxidase activity was lower in AS than Sham. Echocardiogram showed LV concentric hypertrophy and dysfunction before and after treatment. Tissue Doppler imaging (TDI) of mitral annulus systolic velocity and isovolumetric relaxation time were lower in AS than Sham; in AS-NAC these parameters were between those in Sham and AS and did not differ from either group. NAC reduced p-ERK and p-JNK protein expression, attenuated myocyte hypertrophy and myocardial fibrosis, and decreased lung congestion frequency. Conclusion. N-acetylcysteine restores myocardial total glutathione, reduces oxidative stress, improves MAPK signaling, and attenuates myocyte hypertrophy, myocardial fibrosis, and LV dysfunction in aortic stenosis rats.Asociación Universitaria Iberoamericana de Postgrado (AIUP)Universidade Estadual Paulista (Unesp)Okoshi, Marina Politi [UNESP]Damatto, Ricardo Luiz [UNESP]Rosa, Camila Moreno [UNESP]Universidade Estadual Paulista (Unesp)Abreu Reyes, David Rafael [UNESP]2017-10-11T19:08:57Z2017-10-11T19:08:57Z2017-08-14info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdfapplication/pdfhttp://hdl.handle.net/11449/15190500089307233004064020P04463138671998432porinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESP2024-09-03T17:27:39Zoai:repositorio.unesp.br:11449/151905Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-03T17:27:39Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Influência do exercício físico e da N-acetilcisteína no remodelamento cardíaco e estresse oxidativo de ratos com sobrecarga pressórica crônica
N-acetylcysteine influence on oxidative stress and cardiac remodeling in rats during transition from compensated left ventricular hypertrophy to heart failure
title Influência do exercício físico e da N-acetilcisteína no remodelamento cardíaco e estresse oxidativo de ratos com sobrecarga pressórica crônica
spellingShingle Influência do exercício físico e da N-acetilcisteína no remodelamento cardíaco e estresse oxidativo de ratos com sobrecarga pressórica crônica
Abreu Reyes, David Rafael [UNESP]
Exercício físico
Insuficiência cardíaca
N-Acetilcisteína
Ventrículo izquierdo
title_short Influência do exercício físico e da N-acetilcisteína no remodelamento cardíaco e estresse oxidativo de ratos com sobrecarga pressórica crônica
title_full Influência do exercício físico e da N-acetilcisteína no remodelamento cardíaco e estresse oxidativo de ratos com sobrecarga pressórica crônica
title_fullStr Influência do exercício físico e da N-acetilcisteína no remodelamento cardíaco e estresse oxidativo de ratos com sobrecarga pressórica crônica
title_full_unstemmed Influência do exercício físico e da N-acetilcisteína no remodelamento cardíaco e estresse oxidativo de ratos com sobrecarga pressórica crônica
title_sort Influência do exercício físico e da N-acetilcisteína no remodelamento cardíaco e estresse oxidativo de ratos com sobrecarga pressórica crônica
author Abreu Reyes, David Rafael [UNESP]
author_facet Abreu Reyes, David Rafael [UNESP]
author_role author
dc.contributor.none.fl_str_mv Okoshi, Marina Politi [UNESP]
Damatto, Ricardo Luiz [UNESP]
Rosa, Camila Moreno [UNESP]
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Abreu Reyes, David Rafael [UNESP]
dc.subject.por.fl_str_mv Exercício físico
Insuficiência cardíaca
N-Acetilcisteína
Ventrículo izquierdo
topic Exercício físico
Insuficiência cardíaca
N-Acetilcisteína
Ventrículo izquierdo
description Objective. To evaluate the effects of N-acetylcysteine (NAC) on cardiac remodeling in rats during transition from compensated left ventricular (LV) hypertrophy to heart failure. Methods and Results. Four months after inducing aortic stenosis (AS), Wistar rats were assigned into three groups: Sham, AS, and AS treated with NAC (AS-NAC for eight weeks). NAC restored myocardial total glutathione (Sham 20.8±3.00; AS 12.6±2.92; AS-NAC 17.6±2.45 nmol/g tissue; p<0.05 AS vs Sham and AS-NAC). Malondialdehyde serum concentration was lower in AS-NAC and myocardial lipid hydroperoxide was higher in AS (Sham 199±48.1; AS 301±36.0; AS-NAC 181±41.3 nmol/g tissue). Glutathione peroxidase activity was lower in AS than Sham. Echocardiogram showed LV concentric hypertrophy and dysfunction before and after treatment. Tissue Doppler imaging (TDI) of mitral annulus systolic velocity and isovolumetric relaxation time were lower in AS than Sham; in AS-NAC these parameters were between those in Sham and AS and did not differ from either group. NAC reduced p-ERK and p-JNK protein expression, attenuated myocyte hypertrophy and myocardial fibrosis, and decreased lung congestion frequency. Conclusion. N-acetylcysteine restores myocardial total glutathione, reduces oxidative stress, improves MAPK signaling, and attenuates myocyte hypertrophy, myocardial fibrosis, and LV dysfunction in aortic stenosis rats.
publishDate 2017
dc.date.none.fl_str_mv 2017-10-11T19:08:57Z
2017-10-11T19:08:57Z
2017-08-14
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/11449/151905
000893072
33004064020P0
4463138671998432
url http://hdl.handle.net/11449/151905
identifier_str_mv 000893072
33004064020P0
4463138671998432
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual Paulista (Unesp)
publisher.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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