Post-artificial insemination supplementation with calcium salts of soybean oil influences pregnancy establishment factors in Bos indicus beef cows
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.2527/jas2016-0916 http://hdl.handle.net/11449/165451 |
Resumo: | The objective of this experiment was to compare hormonal, uterine, and conceptus factors associated with pregnancy establishment in Bos indicus beef cows supplemented or not with Ca salts of soybean oil (CSSO) for 21 d beginning after timed AI. One hundred lactating multiparous Nelore cows were allocated to 20 groups of 5 cows/group and timed inseminated on d 0 of the experiment. After AI, groups were randomly assigned to receive (as-fed basis) 100 g of protein-mineral mix + 100 g of ground corn per cow per day, in addition to 1) 100 g/cow daily of CSSO (n = 10) or 2) 100 g/cow daily of kaolin (CON; rumen-inert indigestible substance; n = 10). Groups were maintained in 4 Panicum maximum pastures (5 groups from the same treatment within each pasture) with ad libitum access to forage. Groups were segregated daily and individually offered treatments from d 0 to 21. Blood samples were collected and transrectal ultrasonography was performed to verify ovulation and corpus luteum (CL) volume immediately before AI (d 0) and on d 7 and 15. After ultrasonography on d 15, 60 cows (30 cows/treatment and 3 cows/group) diagnosed without the presence of a CL on d 0 but with a CL greater than 0.38 cm3 in volume on d 7 and 15 were assigned to conceptus collection via transcervical flushing with PBS followed by endometrial biopsy in the uterine horn ipsilateral to the CL. Additional blood samples were collected for wholeblood RNA extraction (d 20), and pregnancy status was verified by transrectal ultrasonography (d 30) in cows not assigned to conceptus collection. Cows receiving CSSO had greater (P <= 0.04) mean plasma linoleic acid concentration, plasma linoleic: linolenic acid ratio, plasma progesterone (P4) concentration, and CL volume during the experiment compared with CON cows. Moreover, CSSO supplementation increased (P <= 0.04) length and mRNA expression of prostaglandin E synthase and interferon-tau by the conceptus as well as blood mRNA expression of interferon-stimulated genes on d 20 in gestating cows. No treatment differences were detected (P >= 0.30) for endometrial mRNA expression of prostaglandin E synthase and cyclooxygenase-2. In summary, post-AI CSSO supplementation to B. indicus beef cows increased plasma concentration of linoleic acid and enhanced pregnancy establishment factors, which included CL development and plasma P4 concentration, conceptus growth, and mRNA expression of interferon-tau as well as blood mRNA expression of interferon-stimulated genes. |
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Post-artificial insemination supplementation with calcium salts of soybean oil influences pregnancy establishment factors in Bos indicus beef cowsbeef cowscalcium salts of soybean oilgene expressioninterferon-taupregnancyprogesteroneThe objective of this experiment was to compare hormonal, uterine, and conceptus factors associated with pregnancy establishment in Bos indicus beef cows supplemented or not with Ca salts of soybean oil (CSSO) for 21 d beginning after timed AI. One hundred lactating multiparous Nelore cows were allocated to 20 groups of 5 cows/group and timed inseminated on d 0 of the experiment. After AI, groups were randomly assigned to receive (as-fed basis) 100 g of protein-mineral mix + 100 g of ground corn per cow per day, in addition to 1) 100 g/cow daily of CSSO (n = 10) or 2) 100 g/cow daily of kaolin (CON; rumen-inert indigestible substance; n = 10). Groups were maintained in 4 Panicum maximum pastures (5 groups from the same treatment within each pasture) with ad libitum access to forage. Groups were segregated daily and individually offered treatments from d 0 to 21. Blood samples were collected and transrectal ultrasonography was performed to verify ovulation and corpus luteum (CL) volume immediately before AI (d 0) and on d 7 and 15. After ultrasonography on d 15, 60 cows (30 cows/treatment and 3 cows/group) diagnosed without the presence of a CL on d 0 but with a CL greater than 0.38 cm3 in volume on d 7 and 15 were assigned to conceptus collection via transcervical flushing with PBS followed by endometrial biopsy in the uterine horn ipsilateral to the CL. Additional blood samples were collected for wholeblood RNA extraction (d 20), and pregnancy status was verified by transrectal ultrasonography (d 30) in cows not assigned to conceptus collection. Cows receiving CSSO had greater (P <= 0.04) mean plasma linoleic acid concentration, plasma linoleic: linolenic acid ratio, plasma progesterone (P4) concentration, and CL volume during the experiment compared with CON cows. Moreover, CSSO supplementation increased (P <= 0.04) length and mRNA expression of prostaglandin E synthase and interferon-tau by the conceptus as well as blood mRNA expression of interferon-stimulated genes on d 20 in gestating cows. No treatment differences were detected (P >= 0.30) for endometrial mRNA expression of prostaglandin E synthase and cyclooxygenase-2. In summary, post-AI CSSO supplementation to B. indicus beef cows increased plasma concentration of linoleic acid and enhanced pregnancy establishment factors, which included CL development and plasma P4 concentration, conceptus growth, and mRNA expression of interferon-tau as well as blood mRNA expression of interferon-stimulated genes.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Unisalesiano Auxilium, BR-16016500 Aracatuba, SP, BrazilOregon State Univ, Eastern Oregon Agr Res Ctr, Burns, OR 97720 USAUniv Estadual Paulista, Fac Med Vet & Zootecnia, BR-18618970 Botucatu, SP, BrazilUniv Sao Paulo, ESALQ, Dept Zootecnia, BR-13418900 Piracicaba, SP, BrazilUniv British Columbia, Fac Land & Food Syst, Vancouver, BC V6T 1Z4, CanadaUniv Estadual Paulista, UNESP, Fac Med Vet & Zootecnia, Programa Posgrad Zootecnia, BR-18618970 Botucatu, SP, BrazilUniv Estadual Paulista, Fac Med Vet & Zootecnia, BR-18618970 Botucatu, SP, BrazilUniv Estadual Paulista, UNESP, Fac Med Vet & Zootecnia, Programa Posgrad Zootecnia, BR-18618970 Botucatu, SP, BrazilFAPESP: 2014/14136-4Amer Soc Animal ScienceUnisalesiano AuxiliumOregon State UnivUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Univ British ColumbiaCipriano, R. S.Cooke, R. F. [UNESP]Rodrigues, A. D. [UNESP]Silva, L. G. T. [UNESP]Bohnert, D. W.Marques, R. S.Vasconcelos, J. L. M. [UNESP]Pires, A. V.Cerri, R. L. A.2018-11-28T03:37:23Z2018-11-28T03:37:23Z2016-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article4892-4902application/pdfhttp://dx.doi.org/10.2527/jas2016-0916Journal Of Animal Science. Champaign: Amer Soc Animal Science, v. 94, n. 11, p. 4892-4902, 2016.0021-8812http://hdl.handle.net/11449/16545110.2527/jas2016-0916WOS:000392643800039WOS000392643800039.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal Of Animal Science0,848info:eu-repo/semantics/openAccess2023-10-29T06:10:24Zoai:repositorio.unesp.br:11449/165451Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-29T06:10:24Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Post-artificial insemination supplementation with calcium salts of soybean oil influences pregnancy establishment factors in Bos indicus beef cows |
title |
Post-artificial insemination supplementation with calcium salts of soybean oil influences pregnancy establishment factors in Bos indicus beef cows |
spellingShingle |
Post-artificial insemination supplementation with calcium salts of soybean oil influences pregnancy establishment factors in Bos indicus beef cows Cipriano, R. S. beef cows calcium salts of soybean oil gene expression interferon-tau pregnancy progesterone |
title_short |
Post-artificial insemination supplementation with calcium salts of soybean oil influences pregnancy establishment factors in Bos indicus beef cows |
title_full |
Post-artificial insemination supplementation with calcium salts of soybean oil influences pregnancy establishment factors in Bos indicus beef cows |
title_fullStr |
Post-artificial insemination supplementation with calcium salts of soybean oil influences pregnancy establishment factors in Bos indicus beef cows |
title_full_unstemmed |
Post-artificial insemination supplementation with calcium salts of soybean oil influences pregnancy establishment factors in Bos indicus beef cows |
title_sort |
Post-artificial insemination supplementation with calcium salts of soybean oil influences pregnancy establishment factors in Bos indicus beef cows |
author |
Cipriano, R. S. |
author_facet |
Cipriano, R. S. Cooke, R. F. [UNESP] Rodrigues, A. D. [UNESP] Silva, L. G. T. [UNESP] Bohnert, D. W. Marques, R. S. Vasconcelos, J. L. M. [UNESP] Pires, A. V. Cerri, R. L. A. |
author_role |
author |
author2 |
Cooke, R. F. [UNESP] Rodrigues, A. D. [UNESP] Silva, L. G. T. [UNESP] Bohnert, D. W. Marques, R. S. Vasconcelos, J. L. M. [UNESP] Pires, A. V. Cerri, R. L. A. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Unisalesiano Auxilium Oregon State Univ Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) Univ British Columbia |
dc.contributor.author.fl_str_mv |
Cipriano, R. S. Cooke, R. F. [UNESP] Rodrigues, A. D. [UNESP] Silva, L. G. T. [UNESP] Bohnert, D. W. Marques, R. S. Vasconcelos, J. L. M. [UNESP] Pires, A. V. Cerri, R. L. A. |
dc.subject.por.fl_str_mv |
beef cows calcium salts of soybean oil gene expression interferon-tau pregnancy progesterone |
topic |
beef cows calcium salts of soybean oil gene expression interferon-tau pregnancy progesterone |
description |
The objective of this experiment was to compare hormonal, uterine, and conceptus factors associated with pregnancy establishment in Bos indicus beef cows supplemented or not with Ca salts of soybean oil (CSSO) for 21 d beginning after timed AI. One hundred lactating multiparous Nelore cows were allocated to 20 groups of 5 cows/group and timed inseminated on d 0 of the experiment. After AI, groups were randomly assigned to receive (as-fed basis) 100 g of protein-mineral mix + 100 g of ground corn per cow per day, in addition to 1) 100 g/cow daily of CSSO (n = 10) or 2) 100 g/cow daily of kaolin (CON; rumen-inert indigestible substance; n = 10). Groups were maintained in 4 Panicum maximum pastures (5 groups from the same treatment within each pasture) with ad libitum access to forage. Groups were segregated daily and individually offered treatments from d 0 to 21. Blood samples were collected and transrectal ultrasonography was performed to verify ovulation and corpus luteum (CL) volume immediately before AI (d 0) and on d 7 and 15. After ultrasonography on d 15, 60 cows (30 cows/treatment and 3 cows/group) diagnosed without the presence of a CL on d 0 but with a CL greater than 0.38 cm3 in volume on d 7 and 15 were assigned to conceptus collection via transcervical flushing with PBS followed by endometrial biopsy in the uterine horn ipsilateral to the CL. Additional blood samples were collected for wholeblood RNA extraction (d 20), and pregnancy status was verified by transrectal ultrasonography (d 30) in cows not assigned to conceptus collection. Cows receiving CSSO had greater (P <= 0.04) mean plasma linoleic acid concentration, plasma linoleic: linolenic acid ratio, plasma progesterone (P4) concentration, and CL volume during the experiment compared with CON cows. Moreover, CSSO supplementation increased (P <= 0.04) length and mRNA expression of prostaglandin E synthase and interferon-tau by the conceptus as well as blood mRNA expression of interferon-stimulated genes on d 20 in gestating cows. No treatment differences were detected (P >= 0.30) for endometrial mRNA expression of prostaglandin E synthase and cyclooxygenase-2. In summary, post-AI CSSO supplementation to B. indicus beef cows increased plasma concentration of linoleic acid and enhanced pregnancy establishment factors, which included CL development and plasma P4 concentration, conceptus growth, and mRNA expression of interferon-tau as well as blood mRNA expression of interferon-stimulated genes. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-11-01 2018-11-28T03:37:23Z 2018-11-28T03:37:23Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.2527/jas2016-0916 Journal Of Animal Science. Champaign: Amer Soc Animal Science, v. 94, n. 11, p. 4892-4902, 2016. 0021-8812 http://hdl.handle.net/11449/165451 10.2527/jas2016-0916 WOS:000392643800039 WOS000392643800039.pdf |
url |
http://dx.doi.org/10.2527/jas2016-0916 http://hdl.handle.net/11449/165451 |
identifier_str_mv |
Journal Of Animal Science. Champaign: Amer Soc Animal Science, v. 94, n. 11, p. 4892-4902, 2016. 0021-8812 10.2527/jas2016-0916 WOS:000392643800039 WOS000392643800039.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal Of Animal Science 0,848 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
4892-4902 application/pdf |
dc.publisher.none.fl_str_mv |
Amer Soc Animal Science |
publisher.none.fl_str_mv |
Amer Soc Animal Science |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1799964747180802048 |