Molecular characterization of Toxoplasma gondii and Sarcocystis spp. in raw kibbeh and other meat samples commercialized in Botucatu, Southeastern Brazil

Detalhes bibliográficos
Autor(a) principal: Langoni, Helio [UNESP]
Data de Publicação: 2021
Outros Autores: Generoso, Diego [UNESP], Hayasaka, Ênio Yoshinori [UNESP], Mantovan, Karine Bott [UNESP], Menozzi, Benedito Donizete [UNESP], Richini-pereira, Virgínia Bodelão, Silva, Rodrigo Costa Da
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S1984-29612021051
http://hdl.handle.net/11449/213356
Resumo: Toxoplasmosis occurs worldwide causing economic losses to the animal production and problems to the public health. The study aimed to detect Toxoplasma gondii and Sarcocystis spp.in 141 meat products from commercial meat cuts of pork, beef, and kibbeh sold in commercial markets from Botucatu, SP, Brazil. Samples were bioassayed in mice to isolate the parasite, and the parasite DNA detected by PCR targeting the 529 base pairs repeat element region (PCR-529-bp). All samples resulted negative on bioassay, whereas PCR positive for 9 (6,38%), distributed as 5/48 beef, 3/49 pork, and 1/44 kibbeh. PCR-positive were investigated for the the parasite genotype using multiplex-, nested-, and RFLP-PCR for 11 markers (SAG1, 5’-3’SAG2, alt.SAG2, SAG3, B-TUB, GRA6, L358, c22-8, c29-6, PK1, Apico). Complete genotype was determined on just one PCR-positive sample that matched MAS, TgCkBr89 and TgCkBr147 isolates already identified. In addition, nested- and RFLP-PCR targeting 18S rRNA was run for all PCR-positive samples and, the products, sequenced and aligned to the GenBank at NCBI website. Four samples showed 100% homology with T. gondii (GenBank #L37415.1), three with Sarcocystis hominis (GenBank #AF006471.1), two Sarcocystis cruzi (GenBank #AF176934.1), and one Sarcocystis hirsuta (GenBank #AF006469.1), indicating the circulation of T. gondii and Sarcocystis spp.
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spelling Molecular characterization of Toxoplasma gondii and Sarcocystis spp. in raw kibbeh and other meat samples commercialized in Botucatu, Southeastern BrazilCaracterização molecular de Toxoplasma gondii e Sarcocystis spp. em amostras de quibe cru e de outras carnes comercializadas em Botucatu, Região Sudeste do BrasilToxoplasmosissarcocystosismeat productsfood safetypublic healthmolecular techniquesToxoplasmosesarcocistoseprodutos cárneossegurança alimentarsaúde públicatécnicas molecularesToxoplasmosis occurs worldwide causing economic losses to the animal production and problems to the public health. The study aimed to detect Toxoplasma gondii and Sarcocystis spp.in 141 meat products from commercial meat cuts of pork, beef, and kibbeh sold in commercial markets from Botucatu, SP, Brazil. Samples were bioassayed in mice to isolate the parasite, and the parasite DNA detected by PCR targeting the 529 base pairs repeat element region (PCR-529-bp). All samples resulted negative on bioassay, whereas PCR positive for 9 (6,38%), distributed as 5/48 beef, 3/49 pork, and 1/44 kibbeh. PCR-positive were investigated for the the parasite genotype using multiplex-, nested-, and RFLP-PCR for 11 markers (SAG1, 5’-3’SAG2, alt.SAG2, SAG3, B-TUB, GRA6, L358, c22-8, c29-6, PK1, Apico). Complete genotype was determined on just one PCR-positive sample that matched MAS, TgCkBr89 and TgCkBr147 isolates already identified. In addition, nested- and RFLP-PCR targeting 18S rRNA was run for all PCR-positive samples and, the products, sequenced and aligned to the GenBank at NCBI website. Four samples showed 100% homology with T. gondii (GenBank #L37415.1), three with Sarcocystis hominis (GenBank #AF006471.1), two Sarcocystis cruzi (GenBank #AF176934.1), and one Sarcocystis hirsuta (GenBank #AF006469.1), indicating the circulation of T. gondii and Sarcocystis spp.A toxoplasmose está mundialmente distribuída e causa perdas na produção animal e problemas de saúde pública. Objetivou-se detectar Toxoplasma gondii e Sarcocystis spp. em 141 produtos cárneos de origem suína (49), bovina (48) e de quibe cru (44), comercializados em mercados de Botucatu, SP, Brazil. Realizou-se bioensaio das amostras em camundongos para isolamento do parasita, e detecção do DNA pela reação em cadeia pela polimerase, tendo como alvo a região do elemento repetitivo de 529 pares de bases (PCR-529-bp). Todas as amostras foram negativas ao bioensaio e 9 (6,38%) positivas à PCR, sendo 5/48 bovinas, 3/49 suínas e 1/44 quibe. Determinou-se a genotipagem das amostras positivas pela multiplex-, nested- e RFLP-PCR com 11 marcadores (SAG1, 5’-3’SAG2, alt.SAG2, SAG3, B-TUB, GRA6, L358, c22-8, c29-6, PK1, Apico). Obteve-se genótipo completo em uma amostra, semelhante a outros já identificados (MAS, TgCkBr89 e TgCkBr147). Nested- e RFLP-PCR do gene 18S rRNA das amostras positivas à PCR foram realizadas, e os produtos da nested-PCR, sequenciados e alinhados com dados do GenBank no NCBI. Quatro apresentaram 100% de homologia com T. gondii (L37415.1), duas Sarcocystis hominis (AF006471.1), duas Sarcocystis cruzi (AF176934.1), uma Sarcocystis hirsuta (AF006469.1), indicando a circulação de T. gondii e Sarcocystis spp.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Universidade Estadual Paulista, Faculdade de Medicina Veterinária e ZootecniaInstituto Adolfo Lutz, Laboratório Regional de BauruUniversidade do Oeste PaulistaUniversidade Estadual Paulista, Faculdade de Medicina Veterinária e ZootecniaCNPq: 559471/2010-6Colégio Brasileiro de Parasitologia VeterináriaUniversidade Estadual Paulista (Unesp)Instituto Adolfo LutzUniversidade do Oeste PaulistaLangoni, Helio [UNESP]Generoso, Diego [UNESP]Hayasaka, Ênio Yoshinori [UNESP]Mantovan, Karine Bott [UNESP]Menozzi, Benedito Donizete [UNESP]Richini-pereira, Virgínia BodelãoSilva, Rodrigo Costa Da2021-07-14T10:54:05Z2021-07-14T10:54:05Z2021-06-28info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article-application/pdfhttp://dx.doi.org/10.1590/S1984-29612021051Revista Brasileira de Parasitologia Veterinária. Colégio Brasileiro de Parasitologia Veterinária, v. 30, n. 2, p. -, 2021.0103-846X1984-2961http://hdl.handle.net/11449/21335610.1590/S1984-29612021051S1984-29612021000200319S1984-29612021000200319.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista Brasileira de Parasitologia Veterináriainfo:eu-repo/semantics/openAccess2023-10-14T06:09:15Zoai:repositorio.unesp.br:11449/213356Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-14T06:09:15Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Molecular characterization of Toxoplasma gondii and Sarcocystis spp. in raw kibbeh and other meat samples commercialized in Botucatu, Southeastern Brazil
Caracterização molecular de Toxoplasma gondii e Sarcocystis spp. em amostras de quibe cru e de outras carnes comercializadas em Botucatu, Região Sudeste do Brasil
title Molecular characterization of Toxoplasma gondii and Sarcocystis spp. in raw kibbeh and other meat samples commercialized in Botucatu, Southeastern Brazil
spellingShingle Molecular characterization of Toxoplasma gondii and Sarcocystis spp. in raw kibbeh and other meat samples commercialized in Botucatu, Southeastern Brazil
Langoni, Helio [UNESP]
Toxoplasmosis
sarcocystosis
meat products
food safety
public health
molecular techniques
Toxoplasmose
sarcocistose
produtos cárneos
segurança alimentar
saúde pública
técnicas moleculares
title_short Molecular characterization of Toxoplasma gondii and Sarcocystis spp. in raw kibbeh and other meat samples commercialized in Botucatu, Southeastern Brazil
title_full Molecular characterization of Toxoplasma gondii and Sarcocystis spp. in raw kibbeh and other meat samples commercialized in Botucatu, Southeastern Brazil
title_fullStr Molecular characterization of Toxoplasma gondii and Sarcocystis spp. in raw kibbeh and other meat samples commercialized in Botucatu, Southeastern Brazil
title_full_unstemmed Molecular characterization of Toxoplasma gondii and Sarcocystis spp. in raw kibbeh and other meat samples commercialized in Botucatu, Southeastern Brazil
title_sort Molecular characterization of Toxoplasma gondii and Sarcocystis spp. in raw kibbeh and other meat samples commercialized in Botucatu, Southeastern Brazil
author Langoni, Helio [UNESP]
author_facet Langoni, Helio [UNESP]
Generoso, Diego [UNESP]
Hayasaka, Ênio Yoshinori [UNESP]
Mantovan, Karine Bott [UNESP]
Menozzi, Benedito Donizete [UNESP]
Richini-pereira, Virgínia Bodelão
Silva, Rodrigo Costa Da
author_role author
author2 Generoso, Diego [UNESP]
Hayasaka, Ênio Yoshinori [UNESP]
Mantovan, Karine Bott [UNESP]
Menozzi, Benedito Donizete [UNESP]
Richini-pereira, Virgínia Bodelão
Silva, Rodrigo Costa Da
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Instituto Adolfo Lutz
Universidade do Oeste Paulista
dc.contributor.author.fl_str_mv Langoni, Helio [UNESP]
Generoso, Diego [UNESP]
Hayasaka, Ênio Yoshinori [UNESP]
Mantovan, Karine Bott [UNESP]
Menozzi, Benedito Donizete [UNESP]
Richini-pereira, Virgínia Bodelão
Silva, Rodrigo Costa Da
dc.subject.por.fl_str_mv Toxoplasmosis
sarcocystosis
meat products
food safety
public health
molecular techniques
Toxoplasmose
sarcocistose
produtos cárneos
segurança alimentar
saúde pública
técnicas moleculares
topic Toxoplasmosis
sarcocystosis
meat products
food safety
public health
molecular techniques
Toxoplasmose
sarcocistose
produtos cárneos
segurança alimentar
saúde pública
técnicas moleculares
description Toxoplasmosis occurs worldwide causing economic losses to the animal production and problems to the public health. The study aimed to detect Toxoplasma gondii and Sarcocystis spp.in 141 meat products from commercial meat cuts of pork, beef, and kibbeh sold in commercial markets from Botucatu, SP, Brazil. Samples were bioassayed in mice to isolate the parasite, and the parasite DNA detected by PCR targeting the 529 base pairs repeat element region (PCR-529-bp). All samples resulted negative on bioassay, whereas PCR positive for 9 (6,38%), distributed as 5/48 beef, 3/49 pork, and 1/44 kibbeh. PCR-positive were investigated for the the parasite genotype using multiplex-, nested-, and RFLP-PCR for 11 markers (SAG1, 5’-3’SAG2, alt.SAG2, SAG3, B-TUB, GRA6, L358, c22-8, c29-6, PK1, Apico). Complete genotype was determined on just one PCR-positive sample that matched MAS, TgCkBr89 and TgCkBr147 isolates already identified. In addition, nested- and RFLP-PCR targeting 18S rRNA was run for all PCR-positive samples and, the products, sequenced and aligned to the GenBank at NCBI website. Four samples showed 100% homology with T. gondii (GenBank #L37415.1), three with Sarcocystis hominis (GenBank #AF006471.1), two Sarcocystis cruzi (GenBank #AF176934.1), and one Sarcocystis hirsuta (GenBank #AF006469.1), indicating the circulation of T. gondii and Sarcocystis spp.
publishDate 2021
dc.date.none.fl_str_mv 2021-07-14T10:54:05Z
2021-07-14T10:54:05Z
2021-06-28
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1984-29612021051
Revista Brasileira de Parasitologia Veterinária. Colégio Brasileiro de Parasitologia Veterinária, v. 30, n. 2, p. -, 2021.
0103-846X
1984-2961
http://hdl.handle.net/11449/213356
10.1590/S1984-29612021051
S1984-29612021000200319
S1984-29612021000200319.pdf
url http://dx.doi.org/10.1590/S1984-29612021051
http://hdl.handle.net/11449/213356
identifier_str_mv Revista Brasileira de Parasitologia Veterinária. Colégio Brasileiro de Parasitologia Veterinária, v. 30, n. 2, p. -, 2021.
0103-846X
1984-2961
10.1590/S1984-29612021051
S1984-29612021000200319
S1984-29612021000200319.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Revista Brasileira de Parasitologia Veterinária
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv -
application/pdf
dc.publisher.none.fl_str_mv Colégio Brasileiro de Parasitologia Veterinária
publisher.none.fl_str_mv Colégio Brasileiro de Parasitologia Veterinária
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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