Antibacterial activity of diacetylcurcumin against Staphylococcus aureus results in decreased biofilm and cellular adhesion

• Autor(a) principal: Orlandi Sardi, Janaina de Cassia
• Data de Publicação: 2017
• Outros Autores: Polaquini, Carlos Roberto [UNESP], Freires, Irlan Almeida, Carvalho Galvao, Livia Camara de, Lazarini, Josy Goldoni, Torrezan, Guilherme Silva [UNESP], Regasini, Luis Octavio [UNESP], Rosalen, Pedro Luiz
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1099/jmm.0.000494; http://hdl.handle.net/11449/162897
• topic: diacetylcurcumin; curcumin; Staphylococcus aureus; anti-adhesion; antibiofilm; toxicity

Purpose. Staphylococcus aureus infections have contributed to the global healthcare burden, particularly with regard to hospital-acquired meticillin-resistant S. aureus (MRSA) infections. Methodology. This study describes the antibacterial activity of diacetylcurcumin (DAC) against meticillin-susceptible S. aureus/MRSA biofilm formation, survival, metabolic activity and structure; its ability to prevent bacterial adhesion to human cells; and toxicity in Galleria mellonella larvae. Results. DAC showed excellent antibacterial activity, with MIC ranging between 17.3 and 34.6 mu mol l(-1), and minimum bactericidal concentration ranging between 69 and 277 mu mol l(-1). It significantly reduced bacterial biofilm survival - by 22-63% (at MIC, 10 x MIC or 100 x MIC) as compared to the 25-42% reduction by vancomycin (P<0.0001) - and severely affected biofilm cell structures, leading to damaged architecture and the formation of amorphous cell clusters. Treatment with DAC (MIC/4) decreased bacterial adhesion to HaCaT keratinocytes from 1 to 5 h (P<0.0001). Finally, DAC demonstrated low toxicity in G. mellonella at its effective anti-biofilm concentrations. Conclusion. These findings open new avenues for the study of this curcumin derivative as an excellent prototype with anti-MRSA activity.