Genetic alterations in benign lesions: Chronic gastritis and gastric ulcer
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://www.wjgnet.com/1007-9327/abstract_en.asp?f=625&v=12 http://hdl.handle.net/11449/68765 |
Resumo: | Aim: To investigate the occurrence of chromosome 3, 7, 8, 9, and 17 aneuploidies, TP53 gene deletion and p53 protein expression in chronic gastritis, atrophic gastritis and gastric ulcer, and their association with H pylori infection. Methods: Gastric biopsies from normal mucosa (NM, n = 10), chronic gastritis (CG, n = 38), atrophic gastritis (CAG, n = 13) and gastric ulcer (GU, n = 21) were studied using fluorescence in situ hybridization (FISH) and immunohistochemical assay. A modified Giemsa staining technique and PCR were used to detect H pylori. An association of the gastric pathologies and aneuploidies with H pylori infection was assessed. Results: Aneuploidies were increasingly found from CG (21%) to CAG (31%) and to GU (62%), involving mainly monosomy and trisomy 7, trisomies 7 and 8, and trisomies 7, 8 and 17, respectively. A significant association was found between H pylori infection and aneuploidies in CAG (P = 0.0143) and GU (P = 0.0498). No TP53 deletion was found in these gastric lesions, but p53-positive immunoreactivity was detected in 45% (5/11) and 12% (2/17) of CG and GU cases, respectively. However, there was no significant association between p53 expression and H pylori infection. Conclusion: The occurrence of aneuploidies in benign lesions evidences chromosomal instability in early stages of gastric carcinogenesis associated with H pylori infection, which may confer proliferative advantage. The increase of p53 protein expression in CG and GU may be due to overproduction of the wild-type protein related to an inflammatory response in mucosa. © 2006 The WJG Press. All rights reserved. |
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Genetic alterations in benign lesions: Chronic gastritis and gastric ulcerAneuploidiesGastric ulcerGastritisp53 proteinTP53 geneprotein p53adolescentadultagedaneuploidyatrophic gastritisbacterium detectioncell proliferationchildchromosomal instabilitychromosome 17chromosome 3chromosome 7chromosome 8chromosome 9chronic gastritiscontrolled studycorrelation analysisdisease associationfemalefluorescence in situ hybridizationgene deletionGiemsa stainHelicobacter infectionHelicobacter pylorihumanhuman tissueimmunohistochemistryimmunoreactivityinflammationmajor clinical studymalemonosomy 7nonhumanpolymerase chain reactionprotein expressionprotein synthesisstomach biopsystomach carcinogenesisstomach mucosastomach ulcertrisomytrisomy 17trisomy 7trisomy 8wild typeAdultAgedAged, 80 and overAneuploidyChild, PreschoolChronic DiseaseFemaleGene DeletionGenes, p53Helicobacter InfectionsHumansIn Situ Hybridization, FluorescenceMaleMiddle AgedStomach UlcerTumor Suppressor Protein p53Aim: To investigate the occurrence of chromosome 3, 7, 8, 9, and 17 aneuploidies, TP53 gene deletion and p53 protein expression in chronic gastritis, atrophic gastritis and gastric ulcer, and their association with H pylori infection. Methods: Gastric biopsies from normal mucosa (NM, n = 10), chronic gastritis (CG, n = 38), atrophic gastritis (CAG, n = 13) and gastric ulcer (GU, n = 21) were studied using fluorescence in situ hybridization (FISH) and immunohistochemical assay. A modified Giemsa staining technique and PCR were used to detect H pylori. An association of the gastric pathologies and aneuploidies with H pylori infection was assessed. Results: Aneuploidies were increasingly found from CG (21%) to CAG (31%) and to GU (62%), involving mainly monosomy and trisomy 7, trisomies 7 and 8, and trisomies 7, 8 and 17, respectively. A significant association was found between H pylori infection and aneuploidies in CAG (P = 0.0143) and GU (P = 0.0498). No TP53 deletion was found in these gastric lesions, but p53-positive immunoreactivity was detected in 45% (5/11) and 12% (2/17) of CG and GU cases, respectively. However, there was no significant association between p53 expression and H pylori infection. Conclusion: The occurrence of aneuploidies in benign lesions evidences chromosomal instability in early stages of gastric carcinogenesis associated with H pylori infection, which may confer proliferative advantage. The increase of p53 protein expression in CG and GU may be due to overproduction of the wild-type protein related to an inflammatory response in mucosa. © 2006 The WJG Press. All rights reserved.UNESP - São Paulo State University, Rua Cristovao Colombo 2265, 15054-000 Sao Jose do Rio Preto, SPUNIP - Araçatuba, Araçatuba, SPFaculdades Católicas Salesianas, Araçatuba, SPUNESP - São Paulo State University Department of Biology, Rua Cristovao Colombo 2265, 15054-000 Sao Jose do Rio PretoFAMERP School of Medicine, Sao Jose do Rio Preto, SPUNESP - São Paulo State University, Rua Cristovao Colombo 2265, 15054-000 Sao Jose do Rio Preto, SPUNESP - São Paulo State University Department of Biology, Rua Cristovao Colombo 2265, 15054-000 Sao Jose do Rio PretoUniversidade Estadual Paulista (Unesp)UNIP - AraçatubaFaculdades Católicas SalesianasSchool of MedicineGobbo César, Ana Cristina [UNESP]de Freitas Calmon, Marília [UNESP]Cury, Patrícia MalufCaetano, AlaorBorim, Aldenis AlbanezeSilva, Ana Elizabete [UNESP]2014-05-27T11:21:48Z2014-05-27T11:21:48Z2006-01-28info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article625-629application/pdfhttp://www.wjgnet.com/1007-9327/abstract_en.asp?f=625&v=12World Journal of Gastroenterology, v. 12, n. 4, p. 625-629, 2006.1007-9327http://hdl.handle.net/11449/68765WOS:0002399474000202-s2.0-336449074632-s2.0-33644907463.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengWorld Journal of Gastroenterology3.3001,409info:eu-repo/semantics/openAccess2023-10-06T06:02:52Zoai:repositorio.unesp.br:11449/68765Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-06T06:02:52Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Genetic alterations in benign lesions: Chronic gastritis and gastric ulcer |
title |
Genetic alterations in benign lesions: Chronic gastritis and gastric ulcer |
spellingShingle |
Genetic alterations in benign lesions: Chronic gastritis and gastric ulcer Gobbo César, Ana Cristina [UNESP] Aneuploidies Gastric ulcer Gastritis p53 protein TP53 gene protein p53 adolescent adult aged aneuploidy atrophic gastritis bacterium detection cell proliferation child chromosomal instability chromosome 17 chromosome 3 chromosome 7 chromosome 8 chromosome 9 chronic gastritis controlled study correlation analysis disease association female fluorescence in situ hybridization gene deletion Giemsa stain Helicobacter infection Helicobacter pylori human human tissue immunohistochemistry immunoreactivity inflammation major clinical study male monosomy 7 nonhuman polymerase chain reaction protein expression protein synthesis stomach biopsy stomach carcinogenesis stomach mucosa stomach ulcer trisomy trisomy 17 trisomy 7 trisomy 8 wild type Adult Aged Aged, 80 and over Aneuploidy Child, Preschool Chronic Disease Female Gene Deletion Genes, p53 Helicobacter Infections Humans In Situ Hybridization, Fluorescence Male Middle Aged Stomach Ulcer Tumor Suppressor Protein p53 |
title_short |
Genetic alterations in benign lesions: Chronic gastritis and gastric ulcer |
title_full |
Genetic alterations in benign lesions: Chronic gastritis and gastric ulcer |
title_fullStr |
Genetic alterations in benign lesions: Chronic gastritis and gastric ulcer |
title_full_unstemmed |
Genetic alterations in benign lesions: Chronic gastritis and gastric ulcer |
title_sort |
Genetic alterations in benign lesions: Chronic gastritis and gastric ulcer |
author |
Gobbo César, Ana Cristina [UNESP] |
author_facet |
Gobbo César, Ana Cristina [UNESP] de Freitas Calmon, Marília [UNESP] Cury, Patrícia Maluf Caetano, Alaor Borim, Aldenis Albaneze Silva, Ana Elizabete [UNESP] |
author_role |
author |
author2 |
de Freitas Calmon, Marília [UNESP] Cury, Patrícia Maluf Caetano, Alaor Borim, Aldenis Albaneze Silva, Ana Elizabete [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) UNIP - Araçatuba Faculdades Católicas Salesianas School of Medicine |
dc.contributor.author.fl_str_mv |
Gobbo César, Ana Cristina [UNESP] de Freitas Calmon, Marília [UNESP] Cury, Patrícia Maluf Caetano, Alaor Borim, Aldenis Albaneze Silva, Ana Elizabete [UNESP] |
dc.subject.por.fl_str_mv |
Aneuploidies Gastric ulcer Gastritis p53 protein TP53 gene protein p53 adolescent adult aged aneuploidy atrophic gastritis bacterium detection cell proliferation child chromosomal instability chromosome 17 chromosome 3 chromosome 7 chromosome 8 chromosome 9 chronic gastritis controlled study correlation analysis disease association female fluorescence in situ hybridization gene deletion Giemsa stain Helicobacter infection Helicobacter pylori human human tissue immunohistochemistry immunoreactivity inflammation major clinical study male monosomy 7 nonhuman polymerase chain reaction protein expression protein synthesis stomach biopsy stomach carcinogenesis stomach mucosa stomach ulcer trisomy trisomy 17 trisomy 7 trisomy 8 wild type Adult Aged Aged, 80 and over Aneuploidy Child, Preschool Chronic Disease Female Gene Deletion Genes, p53 Helicobacter Infections Humans In Situ Hybridization, Fluorescence Male Middle Aged Stomach Ulcer Tumor Suppressor Protein p53 |
topic |
Aneuploidies Gastric ulcer Gastritis p53 protein TP53 gene protein p53 adolescent adult aged aneuploidy atrophic gastritis bacterium detection cell proliferation child chromosomal instability chromosome 17 chromosome 3 chromosome 7 chromosome 8 chromosome 9 chronic gastritis controlled study correlation analysis disease association female fluorescence in situ hybridization gene deletion Giemsa stain Helicobacter infection Helicobacter pylori human human tissue immunohistochemistry immunoreactivity inflammation major clinical study male monosomy 7 nonhuman polymerase chain reaction protein expression protein synthesis stomach biopsy stomach carcinogenesis stomach mucosa stomach ulcer trisomy trisomy 17 trisomy 7 trisomy 8 wild type Adult Aged Aged, 80 and over Aneuploidy Child, Preschool Chronic Disease Female Gene Deletion Genes, p53 Helicobacter Infections Humans In Situ Hybridization, Fluorescence Male Middle Aged Stomach Ulcer Tumor Suppressor Protein p53 |
description |
Aim: To investigate the occurrence of chromosome 3, 7, 8, 9, and 17 aneuploidies, TP53 gene deletion and p53 protein expression in chronic gastritis, atrophic gastritis and gastric ulcer, and their association with H pylori infection. Methods: Gastric biopsies from normal mucosa (NM, n = 10), chronic gastritis (CG, n = 38), atrophic gastritis (CAG, n = 13) and gastric ulcer (GU, n = 21) were studied using fluorescence in situ hybridization (FISH) and immunohistochemical assay. A modified Giemsa staining technique and PCR were used to detect H pylori. An association of the gastric pathologies and aneuploidies with H pylori infection was assessed. Results: Aneuploidies were increasingly found from CG (21%) to CAG (31%) and to GU (62%), involving mainly monosomy and trisomy 7, trisomies 7 and 8, and trisomies 7, 8 and 17, respectively. A significant association was found between H pylori infection and aneuploidies in CAG (P = 0.0143) and GU (P = 0.0498). No TP53 deletion was found in these gastric lesions, but p53-positive immunoreactivity was detected in 45% (5/11) and 12% (2/17) of CG and GU cases, respectively. However, there was no significant association between p53 expression and H pylori infection. Conclusion: The occurrence of aneuploidies in benign lesions evidences chromosomal instability in early stages of gastric carcinogenesis associated with H pylori infection, which may confer proliferative advantage. The increase of p53 protein expression in CG and GU may be due to overproduction of the wild-type protein related to an inflammatory response in mucosa. © 2006 The WJG Press. All rights reserved. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-01-28 2014-05-27T11:21:48Z 2014-05-27T11:21:48Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.wjgnet.com/1007-9327/abstract_en.asp?f=625&v=12 World Journal of Gastroenterology, v. 12, n. 4, p. 625-629, 2006. 1007-9327 http://hdl.handle.net/11449/68765 WOS:000239947400020 2-s2.0-33644907463 2-s2.0-33644907463.pdf |
url |
http://www.wjgnet.com/1007-9327/abstract_en.asp?f=625&v=12 http://hdl.handle.net/11449/68765 |
identifier_str_mv |
World Journal of Gastroenterology, v. 12, n. 4, p. 625-629, 2006. 1007-9327 WOS:000239947400020 2-s2.0-33644907463 2-s2.0-33644907463.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
World Journal of Gastroenterology 3.300 1,409 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
625-629 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1799964452055941120 |