Measurement of IL-10 serum levels in BALB/c mice treated with beta-1,3 polyglucose or sulfadiazine and acutely infected by Toxoplasma gondii

Detalhes bibliográficos
Autor(a) principal: Picka, M. C. M. [UNESP]
Data de Publicação: 2005
Outros Autores: Calvi, S. A. [UNESP], Lima, C. R. G. [UNESP], Santos, I. A. T. [UNESP], Marcondes-machado, J. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S1678-91992005000400012
http://hdl.handle.net/11449/211865
Resumo: Acute infection by Toxoplasma gondii leads to suppression of cell-mediated immunity, facilitating chronic infection. One of the causes of immunosuppression is Interleukin-10 (IL-10) production. Glucan is used to stimulate phagocytosis. Our objective was to study IL-10 induction in male BALB/c mice with acute T. gondii BTU-2 strain infection, glucan immunostimulation, and sulfadiazine treatment. Animals were distributed into 7 groups: G1: infected with T. gondii; G2: infected with T. gondii and treated with sulfadiazine; G3: infected with T. gondii and immunostimulated with glucan; G4: infected with T. gondii, immunostimulated with glucan, and treated with sulfadiazine; G5: imunostimulated with glucan; G6: treated with saline; and G7: treated with sulfadiazine. IL-10 levels were determined by ELISA; the highest levels were found in G2, G3 and G4, and the lowest in G1 (p<0.001). Groups G1 to G5 and G7 had substantially higher levels than G6 (p<0.001). In this study, the highest IL-10 levels were found in groups treated with glucan.
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spelling Measurement of IL-10 serum levels in BALB/c mice treated with beta-1,3 polyglucose or sulfadiazine and acutely infected by Toxoplasma gondiiToxoplasma gondiiinterleukin-10glucanimmune responsesulfadiazineAcute infection by Toxoplasma gondii leads to suppression of cell-mediated immunity, facilitating chronic infection. One of the causes of immunosuppression is Interleukin-10 (IL-10) production. Glucan is used to stimulate phagocytosis. Our objective was to study IL-10 induction in male BALB/c mice with acute T. gondii BTU-2 strain infection, glucan immunostimulation, and sulfadiazine treatment. Animals were distributed into 7 groups: G1: infected with T. gondii; G2: infected with T. gondii and treated with sulfadiazine; G3: infected with T. gondii and immunostimulated with glucan; G4: infected with T. gondii, immunostimulated with glucan, and treated with sulfadiazine; G5: imunostimulated with glucan; G6: treated with saline; and G7: treated with sulfadiazine. IL-10 levels were determined by ELISA; the highest levels were found in G2, G3 and G4, and the lowest in G1 (p<0.001). Groups G1 to G5 and G7 had substantially higher levels than G6 (p<0.001). In this study, the highest IL-10 levels were found in groups treated with glucan.Universidade Estadual Paulista, Botucatu School of MedicineUniversidade Estadual Paulista, Botucatu School of MedicineCentro de Estudos de Venenos e Animais PeçonhentosUniversidade Estadual Paulista (Unesp)Picka, M. C. M. [UNESP]Calvi, S. A. [UNESP]Lima, C. R. G. [UNESP]Santos, I. A. T. [UNESP]Marcondes-machado, J. [UNESP]2021-07-14T10:30:52Z2021-07-14T10:30:52Z2005-12info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article540-556application/pdfhttp://dx.doi.org/10.1590/S1678-91992005000400012Journal of Venomous Animals and Toxins including Tropical Diseases. Botucatu, SP, Brazil: Centro de Estudos de Venenos e Animais Peçonhentos, v. 11, n. 4, p. 540-556, 2005.1678-9199http://hdl.handle.net/11449/21186510.1590/S1678-91992005000400012S1678-91992005000400012S1678-91992005000400012.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Venomous Animals and Toxins including Tropical Diseasesinfo:eu-repo/semantics/openAccess2024-01-14T06:21:09Zoai:repositorio.unesp.br:11449/211865Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-14T06:21:09Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Measurement of IL-10 serum levels in BALB/c mice treated with beta-1,3 polyglucose or sulfadiazine and acutely infected by Toxoplasma gondii
title Measurement of IL-10 serum levels in BALB/c mice treated with beta-1,3 polyglucose or sulfadiazine and acutely infected by Toxoplasma gondii
spellingShingle Measurement of IL-10 serum levels in BALB/c mice treated with beta-1,3 polyglucose or sulfadiazine and acutely infected by Toxoplasma gondii
Picka, M. C. M. [UNESP]
Toxoplasma gondii
interleukin-10
glucan
immune response
sulfadiazine
title_short Measurement of IL-10 serum levels in BALB/c mice treated with beta-1,3 polyglucose or sulfadiazine and acutely infected by Toxoplasma gondii
title_full Measurement of IL-10 serum levels in BALB/c mice treated with beta-1,3 polyglucose or sulfadiazine and acutely infected by Toxoplasma gondii
title_fullStr Measurement of IL-10 serum levels in BALB/c mice treated with beta-1,3 polyglucose or sulfadiazine and acutely infected by Toxoplasma gondii
title_full_unstemmed Measurement of IL-10 serum levels in BALB/c mice treated with beta-1,3 polyglucose or sulfadiazine and acutely infected by Toxoplasma gondii
title_sort Measurement of IL-10 serum levels in BALB/c mice treated with beta-1,3 polyglucose or sulfadiazine and acutely infected by Toxoplasma gondii
author Picka, M. C. M. [UNESP]
author_facet Picka, M. C. M. [UNESP]
Calvi, S. A. [UNESP]
Lima, C. R. G. [UNESP]
Santos, I. A. T. [UNESP]
Marcondes-machado, J. [UNESP]
author_role author
author2 Calvi, S. A. [UNESP]
Lima, C. R. G. [UNESP]
Santos, I. A. T. [UNESP]
Marcondes-machado, J. [UNESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Picka, M. C. M. [UNESP]
Calvi, S. A. [UNESP]
Lima, C. R. G. [UNESP]
Santos, I. A. T. [UNESP]
Marcondes-machado, J. [UNESP]
dc.subject.por.fl_str_mv Toxoplasma gondii
interleukin-10
glucan
immune response
sulfadiazine
topic Toxoplasma gondii
interleukin-10
glucan
immune response
sulfadiazine
description Acute infection by Toxoplasma gondii leads to suppression of cell-mediated immunity, facilitating chronic infection. One of the causes of immunosuppression is Interleukin-10 (IL-10) production. Glucan is used to stimulate phagocytosis. Our objective was to study IL-10 induction in male BALB/c mice with acute T. gondii BTU-2 strain infection, glucan immunostimulation, and sulfadiazine treatment. Animals were distributed into 7 groups: G1: infected with T. gondii; G2: infected with T. gondii and treated with sulfadiazine; G3: infected with T. gondii and immunostimulated with glucan; G4: infected with T. gondii, immunostimulated with glucan, and treated with sulfadiazine; G5: imunostimulated with glucan; G6: treated with saline; and G7: treated with sulfadiazine. IL-10 levels were determined by ELISA; the highest levels were found in G2, G3 and G4, and the lowest in G1 (p<0.001). Groups G1 to G5 and G7 had substantially higher levels than G6 (p<0.001). In this study, the highest IL-10 levels were found in groups treated with glucan.
publishDate 2005
dc.date.none.fl_str_mv 2005-12
2021-07-14T10:30:52Z
2021-07-14T10:30:52Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1678-91992005000400012
Journal of Venomous Animals and Toxins including Tropical Diseases. Botucatu, SP, Brazil: Centro de Estudos de Venenos e Animais Peçonhentos, v. 11, n. 4, p. 540-556, 2005.
1678-9199
http://hdl.handle.net/11449/211865
10.1590/S1678-91992005000400012
S1678-91992005000400012
S1678-91992005000400012.pdf
url http://dx.doi.org/10.1590/S1678-91992005000400012
http://hdl.handle.net/11449/211865
identifier_str_mv Journal of Venomous Animals and Toxins including Tropical Diseases. Botucatu, SP, Brazil: Centro de Estudos de Venenos e Animais Peçonhentos, v. 11, n. 4, p. 540-556, 2005.
1678-9199
10.1590/S1678-91992005000400012
S1678-91992005000400012
S1678-91992005000400012.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Venomous Animals and Toxins including Tropical Diseases
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 540-556
application/pdf
dc.publisher.none.fl_str_mv Centro de Estudos de Venenos e Animais Peçonhentos
publisher.none.fl_str_mv Centro de Estudos de Venenos e Animais Peçonhentos
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1799965613914849280

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