Area of Rests of Malassez in Young and Adult Rat Molars: Evidences in the Formation of Large Rests
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1002/ar.20826 http://hdl.handle.net/11449/16588 |
Resumo: | Recently, we have demonstrated that the reduction in the number of rests of Malassez (RM) according to age may be due to epithelial cell death by apoptosis. It is known that, under experimental or pathological conditions, the epithelial cells of RM can proliferate, resulting in the formation of odontogenic cysts and tumors. In this study, the area of RM profiles and the number of profiles of epithelial cell nuclei/RM were investigated in young and adult rats. Fragments containing upper molars of rats aged 29, 45, and 120 days were fixed, decalcified, and embedded for light microscopy. In the sections stained by hematoxylin and eosin, the numerical density of RM profiles/mm(2) of periodontal ligament, the area of each RM profile and the number of epithelial cell nuclei profile/RM were obtained. Moreover, detection of cytokeratin by immunohistochemistry and ultrastructural analysis of RM were carried out. Smaller RM were found in all groups, but the very large RM were only observed in the 120-day-old rats. The presence of cells in close juxtaposition exhibiting cytokeratin filaments in their cytoplasm, including in the large RM, characterized their epithelial nature. The number of epithelial cells/RM varied proportionally to the area. Moreover, epithelial cells in mitosis were found in small and large RM, indicating that cellular proliferation can be responsible for the enlargement of RM, under physiological conditions. The results suggest that a possible disequilibrium between the proliferative and apoptotic processes can be related to the enlargement of some RM in the 120-day-old rats. Anat Rec, 292:285291, 2009. (C) 2008 Wiley-Liss, Inc. |
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Area of Rests of Malassez in Young and Adult Rat Molars: Evidences in the Formation of Large Restsrests of Malassezproliferative activityepithelial cellscytokeratinmorphometryRecently, we have demonstrated that the reduction in the number of rests of Malassez (RM) according to age may be due to epithelial cell death by apoptosis. It is known that, under experimental or pathological conditions, the epithelial cells of RM can proliferate, resulting in the formation of odontogenic cysts and tumors. In this study, the area of RM profiles and the number of profiles of epithelial cell nuclei/RM were investigated in young and adult rats. Fragments containing upper molars of rats aged 29, 45, and 120 days were fixed, decalcified, and embedded for light microscopy. In the sections stained by hematoxylin and eosin, the numerical density of RM profiles/mm(2) of periodontal ligament, the area of each RM profile and the number of epithelial cell nuclei profile/RM were obtained. Moreover, detection of cytokeratin by immunohistochemistry and ultrastructural analysis of RM were carried out. Smaller RM were found in all groups, but the very large RM were only observed in the 120-day-old rats. The presence of cells in close juxtaposition exhibiting cytokeratin filaments in their cytoplasm, including in the large RM, characterized their epithelial nature. The number of epithelial cells/RM varied proportionally to the area. Moreover, epithelial cells in mitosis were found in small and large RM, indicating that cellular proliferation can be responsible for the enlargement of RM, under physiological conditions. The results suggest that a possible disequilibrium between the proliferative and apoptotic processes can be related to the enlargement of some RM in the 120-day-old rats. Anat Rec, 292:285291, 2009. (C) 2008 Wiley-Liss, Inc.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)São Paulo State Univ, UNESP, Sch Dent, Dept Morphol,Lab Histol & Embryol, BR-14801903 Araraquara, SP, BrazilSão Paulo State Univ, UNESP, Sch Dent, Dept Morphol,Lab Histol & Embryol, BR-14801903 Araraquara, SP, BrazilFAPESP: 05/57678-2Wiley-lissUniversidade Estadual Paulista (Unesp)Cerri, Paulo Sérgio [UNESP]Goncalves, Jakobe de Souza [UNESP]Cerri, Estela Sasso [UNESP]2014-05-20T13:46:48Z2014-05-20T13:46:48Z2009-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article285-291http://dx.doi.org/10.1002/ar.20826Anatomical Record-advances In Integrative Anatomy and Evolutionary Biology. Hoboken: Wiley-liss, v. 292, n. 2, p. 285-291, 2009.1932-8486http://hdl.handle.net/11449/1658810.1002/ar.20826WOS:000263189900018327849591120788244556300768413020000-0001-5756-5828Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAnatomical Record-advances In Integrative Anatomy and Evolutionary Biology1.3730,766info:eu-repo/semantics/openAccess2021-10-22T21:02:52Zoai:repositorio.unesp.br:11449/16588Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:10:49.789469Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Area of Rests of Malassez in Young and Adult Rat Molars: Evidences in the Formation of Large Rests |
title |
Area of Rests of Malassez in Young and Adult Rat Molars: Evidences in the Formation of Large Rests |
spellingShingle |
Area of Rests of Malassez in Young and Adult Rat Molars: Evidences in the Formation of Large Rests Cerri, Paulo Sérgio [UNESP] rests of Malassez proliferative activity epithelial cells cytokeratin morphometry |
title_short |
Area of Rests of Malassez in Young and Adult Rat Molars: Evidences in the Formation of Large Rests |
title_full |
Area of Rests of Malassez in Young and Adult Rat Molars: Evidences in the Formation of Large Rests |
title_fullStr |
Area of Rests of Malassez in Young and Adult Rat Molars: Evidences in the Formation of Large Rests |
title_full_unstemmed |
Area of Rests of Malassez in Young and Adult Rat Molars: Evidences in the Formation of Large Rests |
title_sort |
Area of Rests of Malassez in Young and Adult Rat Molars: Evidences in the Formation of Large Rests |
author |
Cerri, Paulo Sérgio [UNESP] |
author_facet |
Cerri, Paulo Sérgio [UNESP] Goncalves, Jakobe de Souza [UNESP] Cerri, Estela Sasso [UNESP] |
author_role |
author |
author2 |
Goncalves, Jakobe de Souza [UNESP] Cerri, Estela Sasso [UNESP] |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Cerri, Paulo Sérgio [UNESP] Goncalves, Jakobe de Souza [UNESP] Cerri, Estela Sasso [UNESP] |
dc.subject.por.fl_str_mv |
rests of Malassez proliferative activity epithelial cells cytokeratin morphometry |
topic |
rests of Malassez proliferative activity epithelial cells cytokeratin morphometry |
description |
Recently, we have demonstrated that the reduction in the number of rests of Malassez (RM) according to age may be due to epithelial cell death by apoptosis. It is known that, under experimental or pathological conditions, the epithelial cells of RM can proliferate, resulting in the formation of odontogenic cysts and tumors. In this study, the area of RM profiles and the number of profiles of epithelial cell nuclei/RM were investigated in young and adult rats. Fragments containing upper molars of rats aged 29, 45, and 120 days were fixed, decalcified, and embedded for light microscopy. In the sections stained by hematoxylin and eosin, the numerical density of RM profiles/mm(2) of periodontal ligament, the area of each RM profile and the number of epithelial cell nuclei profile/RM were obtained. Moreover, detection of cytokeratin by immunohistochemistry and ultrastructural analysis of RM were carried out. Smaller RM were found in all groups, but the very large RM were only observed in the 120-day-old rats. The presence of cells in close juxtaposition exhibiting cytokeratin filaments in their cytoplasm, including in the large RM, characterized their epithelial nature. The number of epithelial cells/RM varied proportionally to the area. Moreover, epithelial cells in mitosis were found in small and large RM, indicating that cellular proliferation can be responsible for the enlargement of RM, under physiological conditions. The results suggest that a possible disequilibrium between the proliferative and apoptotic processes can be related to the enlargement of some RM in the 120-day-old rats. Anat Rec, 292:285291, 2009. (C) 2008 Wiley-Liss, Inc. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-02-01 2014-05-20T13:46:48Z 2014-05-20T13:46:48Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1002/ar.20826 Anatomical Record-advances In Integrative Anatomy and Evolutionary Biology. Hoboken: Wiley-liss, v. 292, n. 2, p. 285-291, 2009. 1932-8486 http://hdl.handle.net/11449/16588 10.1002/ar.20826 WOS:000263189900018 3278495911207882 4455630076841302 0000-0001-5756-5828 |
url |
http://dx.doi.org/10.1002/ar.20826 http://hdl.handle.net/11449/16588 |
identifier_str_mv |
Anatomical Record-advances In Integrative Anatomy and Evolutionary Biology. Hoboken: Wiley-liss, v. 292, n. 2, p. 285-291, 2009. 1932-8486 10.1002/ar.20826 WOS:000263189900018 3278495911207882 4455630076841302 0000-0001-5756-5828 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Anatomical Record-advances In Integrative Anatomy and Evolutionary Biology 1.373 0,766 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
285-291 |
dc.publisher.none.fl_str_mv |
Wiley-liss |
publisher.none.fl_str_mv |
Wiley-liss |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128614797934592 |