Role of 1α,25-Dihydroxyvitamin D3 in Adipogenesis of SGBS Cells: New Insights into Human Preadipocyte Proliferation

Detalhes bibliográficos
Autor(a) principal: Felicidade, Ingrid [UNESP]
Data de Publicação: 2018
Outros Autores: Sartori, Daniele, Coort, Susan L.M., Semprebon, Simone Cristine, Niwa, Andressa Megumi, D'Epiro, Gláucia Fernanda Rocha, Biazi, Bruna Isabela, Marques, Lilian Areal, Evelo, Chris T., Mantovani, Mário Sérgio, Ribeiro, Lúcia Regina [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1159/000491770
http://hdl.handle.net/11449/176678
Resumo: Background/Aims: Compared with non-obese individuals, obese individuals commonly store more vitamin D in adipose tissue. VDR expression in adipose tissue can influence adipogenesis and is therefore a target pathway deserving further study. This study aims to assess the role of 1,25(OH)2D3 in human preadipocyte proliferation and differentiation. Methods: RTCA, MTT, and trypan blue assays were used to assess the effects of 1,25(OH)2D3 on the viability, proliferation, and adipogenic differentiation of SGBS cells. Cell cycle and apoptosis analyses were performed with flow cytometry, triglycerides were quantified, and RT-qPCR was used to assess gene expression. Results: We confirmed that the SGBS cell model is suitable for studying adipogenesis and demonstrated that the differentiation protocol induces cell maturation, thereby increasing the lipid content of cells independently of treatment. 1,25(OH)2D3 treatment had different effects according to the cell stage, indicating different modes of action driving proliferation and differentiation. In preadipocytes, 1,25(OH)2D3 induced G1 growth arrest at both tested concentrations without altering CDKN1A gene expression. Treatment with 100 nM 1,25(OH)2D3 also decreased MTT absorbance and the lipid concentration. Moreover, increased normalized cell index values and decreased metabolic activity were not induced by proliferation or apoptosis. Exposure to 100 nM 1,25(OH)2D3 induced VDR, CEBPA, and CEBPB expression, even in the preadipocyte stage. During adipogenesis, 1,25(OH)2D3 had limited effects on processes such as VDR and PPARG gene expression, but it upregulated CEBPA expression. Conclusions: We demonstrated for the first time that 1,25(OH)2D3 induces changes in preadipocytes, including VDR expression and growth arrest, and increases the lipid content in adipocytes treated for 16 days. Preadipocytes are important cells in adipose tissue homeostasis, and understanding the role of 1,25(OH)2D3 in adipogenesis is a crucial step in ensuring adequate vitamin D supplementation, especially for obese individuals.
id UNSP_5d2e95c97548c1b8284bc9c8f5045ca4
oai_identifier_str oai:repositorio.unesp.br:11449/176678
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Role of 1α,25-Dihydroxyvitamin D3 in Adipogenesis of SGBS Cells: New Insights into Human Preadipocyte ProliferationAdipogenesisPreadipocyteSGBSVitamin DBackground/Aims: Compared with non-obese individuals, obese individuals commonly store more vitamin D in adipose tissue. VDR expression in adipose tissue can influence adipogenesis and is therefore a target pathway deserving further study. This study aims to assess the role of 1,25(OH)2D3 in human preadipocyte proliferation and differentiation. Methods: RTCA, MTT, and trypan blue assays were used to assess the effects of 1,25(OH)2D3 on the viability, proliferation, and adipogenic differentiation of SGBS cells. Cell cycle and apoptosis analyses were performed with flow cytometry, triglycerides were quantified, and RT-qPCR was used to assess gene expression. Results: We confirmed that the SGBS cell model is suitable for studying adipogenesis and demonstrated that the differentiation protocol induces cell maturation, thereby increasing the lipid content of cells independently of treatment. 1,25(OH)2D3 treatment had different effects according to the cell stage, indicating different modes of action driving proliferation and differentiation. In preadipocytes, 1,25(OH)2D3 induced G1 growth arrest at both tested concentrations without altering CDKN1A gene expression. Treatment with 100 nM 1,25(OH)2D3 also decreased MTT absorbance and the lipid concentration. Moreover, increased normalized cell index values and decreased metabolic activity were not induced by proliferation or apoptosis. Exposure to 100 nM 1,25(OH)2D3 induced VDR, CEBPA, and CEBPB expression, even in the preadipocyte stage. During adipogenesis, 1,25(OH)2D3 had limited effects on processes such as VDR and PPARG gene expression, but it upregulated CEBPA expression. Conclusions: We demonstrated for the first time that 1,25(OH)2D3 induces changes in preadipocytes, including VDR expression and growth arrest, and increases the lipid content in adipocytes treated for 16 days. Preadipocytes are important cells in adipose tissue homeostasis, and understanding the role of 1,25(OH)2D3 in adipogenesis is a crucial step in ensuring adequate vitamin D supplementation, especially for obese individuals.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Financiadora de Estudos e ProjetosSão Paulo State University (UNESP) School of Medicine Department of PathologyMaastricht University Department of Bioinformatics - BiGCaT NUTRIM School for Nutrition and Translational Research in MetabolismState University of Londrina (UEL) Department of Biochemistry and BiotechnologyState University of Londrina (UEL) Department of General BiologyMaastricht University Maastricht Centre for Systems Biology (MaCSBio)Centro de Ciências Bio. Dept. de Bio. Geral Lab. de Genética Toxicológica Univ. Estadual de Londrina Rodovia Celso Garcia Cid, PR 445 Km 380, Campus UniversitárioSão Paulo State University (UNESP) School of Medicine Department of PathologyCNPq: 150067/2017-8CAPES: 9933/2014-00Universidade Estadual Paulista (Unesp)NUTRIM School for Nutrition and Translational Research in MetabolismUniversidade Estadual de Londrina (UEL)Maastricht Centre for Systems Biology (MaCSBio)Univ. Estadual de Londrina Rodovia Celso Garcia CidFelicidade, Ingrid [UNESP]Sartori, DanieleCoort, Susan L.M.Semprebon, Simone CristineNiwa, Andressa MegumiD'Epiro, Gláucia Fernanda RochaBiazi, Bruna IsabelaMarques, Lilian ArealEvelo, Chris T.Mantovani, Mário SérgioRibeiro, Lúcia Regina [UNESP]2018-12-11T17:22:02Z2018-12-11T17:22:02Z2018-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article407-418application/pdfhttp://dx.doi.org/10.1159/000491770Cellular Physiology and Biochemistry, v. 48, n. 1, p. 407-418, 2018.1421-97781015-8987http://hdl.handle.net/11449/17667810.1159/0004917702-s2.0-850510692742-s2.0-85051069274.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCellular Physiology and Biochemistry1,5611,561info:eu-repo/semantics/openAccess2024-09-03T13:18:33Zoai:repositorio.unesp.br:11449/176678Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-03T13:18:33Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Role of 1α,25-Dihydroxyvitamin D3 in Adipogenesis of SGBS Cells: New Insights into Human Preadipocyte Proliferation
title Role of 1α,25-Dihydroxyvitamin D3 in Adipogenesis of SGBS Cells: New Insights into Human Preadipocyte Proliferation
spellingShingle Role of 1α,25-Dihydroxyvitamin D3 in Adipogenesis of SGBS Cells: New Insights into Human Preadipocyte Proliferation
Felicidade, Ingrid [UNESP]
Adipogenesis
Preadipocyte
SGBS
Vitamin D
title_short Role of 1α,25-Dihydroxyvitamin D3 in Adipogenesis of SGBS Cells: New Insights into Human Preadipocyte Proliferation
title_full Role of 1α,25-Dihydroxyvitamin D3 in Adipogenesis of SGBS Cells: New Insights into Human Preadipocyte Proliferation
title_fullStr Role of 1α,25-Dihydroxyvitamin D3 in Adipogenesis of SGBS Cells: New Insights into Human Preadipocyte Proliferation
title_full_unstemmed Role of 1α,25-Dihydroxyvitamin D3 in Adipogenesis of SGBS Cells: New Insights into Human Preadipocyte Proliferation
title_sort Role of 1α,25-Dihydroxyvitamin D3 in Adipogenesis of SGBS Cells: New Insights into Human Preadipocyte Proliferation
author Felicidade, Ingrid [UNESP]
author_facet Felicidade, Ingrid [UNESP]
Sartori, Daniele
Coort, Susan L.M.
Semprebon, Simone Cristine
Niwa, Andressa Megumi
D'Epiro, Gláucia Fernanda Rocha
Biazi, Bruna Isabela
Marques, Lilian Areal
Evelo, Chris T.
Mantovani, Mário Sérgio
Ribeiro, Lúcia Regina [UNESP]
author_role author
author2 Sartori, Daniele
Coort, Susan L.M.
Semprebon, Simone Cristine
Niwa, Andressa Megumi
D'Epiro, Gláucia Fernanda Rocha
Biazi, Bruna Isabela
Marques, Lilian Areal
Evelo, Chris T.
Mantovani, Mário Sérgio
Ribeiro, Lúcia Regina [UNESP]
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
NUTRIM School for Nutrition and Translational Research in Metabolism
Universidade Estadual de Londrina (UEL)
Maastricht Centre for Systems Biology (MaCSBio)
Univ. Estadual de Londrina Rodovia Celso Garcia Cid
dc.contributor.author.fl_str_mv Felicidade, Ingrid [UNESP]
Sartori, Daniele
Coort, Susan L.M.
Semprebon, Simone Cristine
Niwa, Andressa Megumi
D'Epiro, Gláucia Fernanda Rocha
Biazi, Bruna Isabela
Marques, Lilian Areal
Evelo, Chris T.
Mantovani, Mário Sérgio
Ribeiro, Lúcia Regina [UNESP]
dc.subject.por.fl_str_mv Adipogenesis
Preadipocyte
SGBS
Vitamin D
topic Adipogenesis
Preadipocyte
SGBS
Vitamin D
description Background/Aims: Compared with non-obese individuals, obese individuals commonly store more vitamin D in adipose tissue. VDR expression in adipose tissue can influence adipogenesis and is therefore a target pathway deserving further study. This study aims to assess the role of 1,25(OH)2D3 in human preadipocyte proliferation and differentiation. Methods: RTCA, MTT, and trypan blue assays were used to assess the effects of 1,25(OH)2D3 on the viability, proliferation, and adipogenic differentiation of SGBS cells. Cell cycle and apoptosis analyses were performed with flow cytometry, triglycerides were quantified, and RT-qPCR was used to assess gene expression. Results: We confirmed that the SGBS cell model is suitable for studying adipogenesis and demonstrated that the differentiation protocol induces cell maturation, thereby increasing the lipid content of cells independently of treatment. 1,25(OH)2D3 treatment had different effects according to the cell stage, indicating different modes of action driving proliferation and differentiation. In preadipocytes, 1,25(OH)2D3 induced G1 growth arrest at both tested concentrations without altering CDKN1A gene expression. Treatment with 100 nM 1,25(OH)2D3 also decreased MTT absorbance and the lipid concentration. Moreover, increased normalized cell index values and decreased metabolic activity were not induced by proliferation or apoptosis. Exposure to 100 nM 1,25(OH)2D3 induced VDR, CEBPA, and CEBPB expression, even in the preadipocyte stage. During adipogenesis, 1,25(OH)2D3 had limited effects on processes such as VDR and PPARG gene expression, but it upregulated CEBPA expression. Conclusions: We demonstrated for the first time that 1,25(OH)2D3 induces changes in preadipocytes, including VDR expression and growth arrest, and increases the lipid content in adipocytes treated for 16 days. Preadipocytes are important cells in adipose tissue homeostasis, and understanding the role of 1,25(OH)2D3 in adipogenesis is a crucial step in ensuring adequate vitamin D supplementation, especially for obese individuals.
publishDate 2018
dc.date.none.fl_str_mv 2018-12-11T17:22:02Z
2018-12-11T17:22:02Z
2018-08-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1159/000491770
Cellular Physiology and Biochemistry, v. 48, n. 1, p. 407-418, 2018.
1421-9778
1015-8987
http://hdl.handle.net/11449/176678
10.1159/000491770
2-s2.0-85051069274
2-s2.0-85051069274.pdf
url http://dx.doi.org/10.1159/000491770
http://hdl.handle.net/11449/176678
identifier_str_mv Cellular Physiology and Biochemistry, v. 48, n. 1, p. 407-418, 2018.
1421-9778
1015-8987
10.1159/000491770
2-s2.0-85051069274
2-s2.0-85051069274.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Cellular Physiology and Biochemistry
1,561
1,561
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 407-418
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
_version_ 1810021419545788416