Role of 1α,25-Dihydroxyvitamin D3 in Adipogenesis of SGBS Cells: New Insights into Human Preadipocyte Proliferation
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1159/000491770 http://hdl.handle.net/11449/176678 |
Resumo: | Background/Aims: Compared with non-obese individuals, obese individuals commonly store more vitamin D in adipose tissue. VDR expression in adipose tissue can influence adipogenesis and is therefore a target pathway deserving further study. This study aims to assess the role of 1,25(OH)2D3 in human preadipocyte proliferation and differentiation. Methods: RTCA, MTT, and trypan blue assays were used to assess the effects of 1,25(OH)2D3 on the viability, proliferation, and adipogenic differentiation of SGBS cells. Cell cycle and apoptosis analyses were performed with flow cytometry, triglycerides were quantified, and RT-qPCR was used to assess gene expression. Results: We confirmed that the SGBS cell model is suitable for studying adipogenesis and demonstrated that the differentiation protocol induces cell maturation, thereby increasing the lipid content of cells independently of treatment. 1,25(OH)2D3 treatment had different effects according to the cell stage, indicating different modes of action driving proliferation and differentiation. In preadipocytes, 1,25(OH)2D3 induced G1 growth arrest at both tested concentrations without altering CDKN1A gene expression. Treatment with 100 nM 1,25(OH)2D3 also decreased MTT absorbance and the lipid concentration. Moreover, increased normalized cell index values and decreased metabolic activity were not induced by proliferation or apoptosis. Exposure to 100 nM 1,25(OH)2D3 induced VDR, CEBPA, and CEBPB expression, even in the preadipocyte stage. During adipogenesis, 1,25(OH)2D3 had limited effects on processes such as VDR and PPARG gene expression, but it upregulated CEBPA expression. Conclusions: We demonstrated for the first time that 1,25(OH)2D3 induces changes in preadipocytes, including VDR expression and growth arrest, and increases the lipid content in adipocytes treated for 16 days. Preadipocytes are important cells in adipose tissue homeostasis, and understanding the role of 1,25(OH)2D3 in adipogenesis is a crucial step in ensuring adequate vitamin D supplementation, especially for obese individuals. |
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Role of 1α,25-Dihydroxyvitamin D3 in Adipogenesis of SGBS Cells: New Insights into Human Preadipocyte ProliferationAdipogenesisPreadipocyteSGBSVitamin DBackground/Aims: Compared with non-obese individuals, obese individuals commonly store more vitamin D in adipose tissue. VDR expression in adipose tissue can influence adipogenesis and is therefore a target pathway deserving further study. This study aims to assess the role of 1,25(OH)2D3 in human preadipocyte proliferation and differentiation. Methods: RTCA, MTT, and trypan blue assays were used to assess the effects of 1,25(OH)2D3 on the viability, proliferation, and adipogenic differentiation of SGBS cells. Cell cycle and apoptosis analyses were performed with flow cytometry, triglycerides were quantified, and RT-qPCR was used to assess gene expression. Results: We confirmed that the SGBS cell model is suitable for studying adipogenesis and demonstrated that the differentiation protocol induces cell maturation, thereby increasing the lipid content of cells independently of treatment. 1,25(OH)2D3 treatment had different effects according to the cell stage, indicating different modes of action driving proliferation and differentiation. In preadipocytes, 1,25(OH)2D3 induced G1 growth arrest at both tested concentrations without altering CDKN1A gene expression. Treatment with 100 nM 1,25(OH)2D3 also decreased MTT absorbance and the lipid concentration. Moreover, increased normalized cell index values and decreased metabolic activity were not induced by proliferation or apoptosis. Exposure to 100 nM 1,25(OH)2D3 induced VDR, CEBPA, and CEBPB expression, even in the preadipocyte stage. During adipogenesis, 1,25(OH)2D3 had limited effects on processes such as VDR and PPARG gene expression, but it upregulated CEBPA expression. Conclusions: We demonstrated for the first time that 1,25(OH)2D3 induces changes in preadipocytes, including VDR expression and growth arrest, and increases the lipid content in adipocytes treated for 16 days. Preadipocytes are important cells in adipose tissue homeostasis, and understanding the role of 1,25(OH)2D3 in adipogenesis is a crucial step in ensuring adequate vitamin D supplementation, especially for obese individuals.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Financiadora de Estudos e ProjetosSão Paulo State University (UNESP) School of Medicine Department of PathologyMaastricht University Department of Bioinformatics - BiGCaT NUTRIM School for Nutrition and Translational Research in MetabolismState University of Londrina (UEL) Department of Biochemistry and BiotechnologyState University of Londrina (UEL) Department of General BiologyMaastricht University Maastricht Centre for Systems Biology (MaCSBio)Centro de Ciências Bio. Dept. de Bio. Geral Lab. de Genética Toxicológica Univ. Estadual de Londrina Rodovia Celso Garcia Cid, PR 445 Km 380, Campus UniversitárioSão Paulo State University (UNESP) School of Medicine Department of PathologyCNPq: 150067/2017-8CAPES: 9933/2014-00Universidade Estadual Paulista (Unesp)NUTRIM School for Nutrition and Translational Research in MetabolismUniversidade Estadual de Londrina (UEL)Maastricht Centre for Systems Biology (MaCSBio)Univ. Estadual de Londrina Rodovia Celso Garcia CidFelicidade, Ingrid [UNESP]Sartori, DanieleCoort, Susan L.M.Semprebon, Simone CristineNiwa, Andressa MegumiD'Epiro, Gláucia Fernanda RochaBiazi, Bruna IsabelaMarques, Lilian ArealEvelo, Chris T.Mantovani, Mário SérgioRibeiro, Lúcia Regina [UNESP]2018-12-11T17:22:02Z2018-12-11T17:22:02Z2018-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article407-418application/pdfhttp://dx.doi.org/10.1159/000491770Cellular Physiology and Biochemistry, v. 48, n. 1, p. 407-418, 2018.1421-97781015-8987http://hdl.handle.net/11449/17667810.1159/0004917702-s2.0-850510692742-s2.0-85051069274.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCellular Physiology and Biochemistry1,5611,561info:eu-repo/semantics/openAccess2024-09-03T13:18:33Zoai:repositorio.unesp.br:11449/176678Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-03T13:18:33Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Role of 1α,25-Dihydroxyvitamin D3 in Adipogenesis of SGBS Cells: New Insights into Human Preadipocyte Proliferation |
title |
Role of 1α,25-Dihydroxyvitamin D3 in Adipogenesis of SGBS Cells: New Insights into Human Preadipocyte Proliferation |
spellingShingle |
Role of 1α,25-Dihydroxyvitamin D3 in Adipogenesis of SGBS Cells: New Insights into Human Preadipocyte Proliferation Felicidade, Ingrid [UNESP] Adipogenesis Preadipocyte SGBS Vitamin D |
title_short |
Role of 1α,25-Dihydroxyvitamin D3 in Adipogenesis of SGBS Cells: New Insights into Human Preadipocyte Proliferation |
title_full |
Role of 1α,25-Dihydroxyvitamin D3 in Adipogenesis of SGBS Cells: New Insights into Human Preadipocyte Proliferation |
title_fullStr |
Role of 1α,25-Dihydroxyvitamin D3 in Adipogenesis of SGBS Cells: New Insights into Human Preadipocyte Proliferation |
title_full_unstemmed |
Role of 1α,25-Dihydroxyvitamin D3 in Adipogenesis of SGBS Cells: New Insights into Human Preadipocyte Proliferation |
title_sort |
Role of 1α,25-Dihydroxyvitamin D3 in Adipogenesis of SGBS Cells: New Insights into Human Preadipocyte Proliferation |
author |
Felicidade, Ingrid [UNESP] |
author_facet |
Felicidade, Ingrid [UNESP] Sartori, Daniele Coort, Susan L.M. Semprebon, Simone Cristine Niwa, Andressa Megumi D'Epiro, Gláucia Fernanda Rocha Biazi, Bruna Isabela Marques, Lilian Areal Evelo, Chris T. Mantovani, Mário Sérgio Ribeiro, Lúcia Regina [UNESP] |
author_role |
author |
author2 |
Sartori, Daniele Coort, Susan L.M. Semprebon, Simone Cristine Niwa, Andressa Megumi D'Epiro, Gláucia Fernanda Rocha Biazi, Bruna Isabela Marques, Lilian Areal Evelo, Chris T. Mantovani, Mário Sérgio Ribeiro, Lúcia Regina [UNESP] |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) NUTRIM School for Nutrition and Translational Research in Metabolism Universidade Estadual de Londrina (UEL) Maastricht Centre for Systems Biology (MaCSBio) Univ. Estadual de Londrina Rodovia Celso Garcia Cid |
dc.contributor.author.fl_str_mv |
Felicidade, Ingrid [UNESP] Sartori, Daniele Coort, Susan L.M. Semprebon, Simone Cristine Niwa, Andressa Megumi D'Epiro, Gláucia Fernanda Rocha Biazi, Bruna Isabela Marques, Lilian Areal Evelo, Chris T. Mantovani, Mário Sérgio Ribeiro, Lúcia Regina [UNESP] |
dc.subject.por.fl_str_mv |
Adipogenesis Preadipocyte SGBS Vitamin D |
topic |
Adipogenesis Preadipocyte SGBS Vitamin D |
description |
Background/Aims: Compared with non-obese individuals, obese individuals commonly store more vitamin D in adipose tissue. VDR expression in adipose tissue can influence adipogenesis and is therefore a target pathway deserving further study. This study aims to assess the role of 1,25(OH)2D3 in human preadipocyte proliferation and differentiation. Methods: RTCA, MTT, and trypan blue assays were used to assess the effects of 1,25(OH)2D3 on the viability, proliferation, and adipogenic differentiation of SGBS cells. Cell cycle and apoptosis analyses were performed with flow cytometry, triglycerides were quantified, and RT-qPCR was used to assess gene expression. Results: We confirmed that the SGBS cell model is suitable for studying adipogenesis and demonstrated that the differentiation protocol induces cell maturation, thereby increasing the lipid content of cells independently of treatment. 1,25(OH)2D3 treatment had different effects according to the cell stage, indicating different modes of action driving proliferation and differentiation. In preadipocytes, 1,25(OH)2D3 induced G1 growth arrest at both tested concentrations without altering CDKN1A gene expression. Treatment with 100 nM 1,25(OH)2D3 also decreased MTT absorbance and the lipid concentration. Moreover, increased normalized cell index values and decreased metabolic activity were not induced by proliferation or apoptosis. Exposure to 100 nM 1,25(OH)2D3 induced VDR, CEBPA, and CEBPB expression, even in the preadipocyte stage. During adipogenesis, 1,25(OH)2D3 had limited effects on processes such as VDR and PPARG gene expression, but it upregulated CEBPA expression. Conclusions: We demonstrated for the first time that 1,25(OH)2D3 induces changes in preadipocytes, including VDR expression and growth arrest, and increases the lipid content in adipocytes treated for 16 days. Preadipocytes are important cells in adipose tissue homeostasis, and understanding the role of 1,25(OH)2D3 in adipogenesis is a crucial step in ensuring adequate vitamin D supplementation, especially for obese individuals. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T17:22:02Z 2018-12-11T17:22:02Z 2018-08-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1159/000491770 Cellular Physiology and Biochemistry, v. 48, n. 1, p. 407-418, 2018. 1421-9778 1015-8987 http://hdl.handle.net/11449/176678 10.1159/000491770 2-s2.0-85051069274 2-s2.0-85051069274.pdf |
url |
http://dx.doi.org/10.1159/000491770 http://hdl.handle.net/11449/176678 |
identifier_str_mv |
Cellular Physiology and Biochemistry, v. 48, n. 1, p. 407-418, 2018. 1421-9778 1015-8987 10.1159/000491770 2-s2.0-85051069274 2-s2.0-85051069274.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Cellular Physiology and Biochemistry 1,561 1,561 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
407-418 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1810021419545788416 |