Effects of ammonia stress in the Amazon river shrimp Macrobrachium amazonicum (Decapoda, Palaemonidae)
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.aquatox.2015.10.021 http://hdl.handle.net/11449/158678 |
Resumo: | We evaluate the effects of total ammonia nitrogen-N (TAN) exposure for 72 h on (Na+,K+)- and V(H+)-ATPase activities and on their subunit expressions in gills of the diadromous freshwater shrimp Macrobrachium amazonicum. Specific (Na+,K+)- and V(H+)-ATPase activities increased roughly 1.5- to 2 fold, respectively, after exposure to 2.0 mmol L-1 TAN. Quantitative RT-PCR analyses revealed a 2.5-fold increase in V(H+)-ATPase B subunit mRNA expression while (Na+,K+)-ATPase alpha-subunit expression was unchanged. Immunohistochemical analyses of the gill lamellae located the (Na+,K+)-ATPase throughout the intralamellar septal cells, independently of TAN concentration, while the V(H+)-ATPase was located in both the apical pillar cell flanges and pillar cell bodies. Systemic stress parameters like total hemocyte count decreased by 30% after exposure to 2.0 mmol L-1 TAN, accompanied by increased activities of the oxidative stress enzymes superoxide dismutase, glutathione reductase and glucose-6-phosphate dehydrogenase in the gills. The stress responses of M. amazonicum to elevated TAN include increases in gill (Na+,K+)- and V(H+)-ATPase activities that are accompanied by changes in oxidative stress enzyme activities, immune system effects and an increase in gill V(H+)-ATPase gene expression. These findings likely underpin physiological effects in a crustacean like M. amazonicum that exploits multiple ecosystems during its life cycle, as well as under culture conditions that may significantly impact shrimp production by the aquaculture industry. (C) 2015 Elsevier B.V. All rights reserved. |
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Effects of ammonia stress in the Amazon river shrimp Macrobrachium amazonicum (Decapoda, Palaemonidae)Ammonia exposureGill microsome(Na+, K+)-ATPaseV(H+)-ATPaseGene expressionOxidative enzymesMacrobrachium amazonicumWe evaluate the effects of total ammonia nitrogen-N (TAN) exposure for 72 h on (Na+,K+)- and V(H+)-ATPase activities and on their subunit expressions in gills of the diadromous freshwater shrimp Macrobrachium amazonicum. Specific (Na+,K+)- and V(H+)-ATPase activities increased roughly 1.5- to 2 fold, respectively, after exposure to 2.0 mmol L-1 TAN. Quantitative RT-PCR analyses revealed a 2.5-fold increase in V(H+)-ATPase B subunit mRNA expression while (Na+,K+)-ATPase alpha-subunit expression was unchanged. Immunohistochemical analyses of the gill lamellae located the (Na+,K+)-ATPase throughout the intralamellar septal cells, independently of TAN concentration, while the V(H+)-ATPase was located in both the apical pillar cell flanges and pillar cell bodies. Systemic stress parameters like total hemocyte count decreased by 30% after exposure to 2.0 mmol L-1 TAN, accompanied by increased activities of the oxidative stress enzymes superoxide dismutase, glutathione reductase and glucose-6-phosphate dehydrogenase in the gills. The stress responses of M. amazonicum to elevated TAN include increases in gill (Na+,K+)- and V(H+)-ATPase activities that are accompanied by changes in oxidative stress enzyme activities, immune system effects and an increase in gill V(H+)-ATPase gene expression. These findings likely underpin physiological effects in a crustacean like M. amazonicum that exploits multiple ecosystems during its life cycle, as well as under culture conditions that may significantly impact shrimp production by the aquaculture industry. (C) 2015 Elsevier B.V. All rights reserved.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Fundacao de Amparo a Pesquisa do Estado do AmazonasUniv Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Quim, Ave Bandeirantes 3900, BR-14040901 Ribeirao Preto, SP, BrazilUniv Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Biol, BR-14040901 Ribeirao Preto, SP, BrazilUniv Estadual Paulista, Campus Sao Jose Rio Preto, Sao Paulo, BrazilUniv Estadual Paulista, Campus Sao Jose Rio Preto, Sao Paulo, BrazilCNPq: CNPq 560501/2101-2FAPESP: 2010/17534-0FAPESP: 2011/022537-0FAPESP: 2013/22605-1Fundacao de Amparo a Pesquisa do Estado do Amazonas: INCT/CNPq/FAPEAM 573976/2008-2CNPq: 560501/2010-2CNPq: 302776/2011-7CNPq: 300564/2013-9CNPq: 307603/2014-8FAPESP: 2013/24252-9FAPESP: 2011/15776-9Elsevier B.V.Universidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Pinto, Marcelo R.Lucena, Malson N.Faleiros, Rogerio OliveiraAlmeida, Eduardo Alves [UNESP]McNamara, John C.Leone, Francisco A.2018-11-26T15:28:35Z2018-11-26T15:28:35Z2016-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article13-23application/pdfhttp://dx.doi.org/10.1016/j.aquatox.2015.10.021Aquatic Toxicology. Amsterdam: Elsevier Science Bv, v. 170, p. 13-23, 2016.0166-445Xhttp://hdl.handle.net/11449/15867810.1016/j.aquatox.2015.10.021WOS:000368564500002WOS000368564500002.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAquatic Toxicologyinfo:eu-repo/semantics/openAccess2023-12-28T06:15:26Zoai:repositorio.unesp.br:11449/158678Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-28T06:15:26Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effects of ammonia stress in the Amazon river shrimp Macrobrachium amazonicum (Decapoda, Palaemonidae) |
title |
Effects of ammonia stress in the Amazon river shrimp Macrobrachium amazonicum (Decapoda, Palaemonidae) |
spellingShingle |
Effects of ammonia stress in the Amazon river shrimp Macrobrachium amazonicum (Decapoda, Palaemonidae) Pinto, Marcelo R. Ammonia exposure Gill microsome (Na+, K+)-ATPase V(H+)-ATPase Gene expression Oxidative enzymes Macrobrachium amazonicum |
title_short |
Effects of ammonia stress in the Amazon river shrimp Macrobrachium amazonicum (Decapoda, Palaemonidae) |
title_full |
Effects of ammonia stress in the Amazon river shrimp Macrobrachium amazonicum (Decapoda, Palaemonidae) |
title_fullStr |
Effects of ammonia stress in the Amazon river shrimp Macrobrachium amazonicum (Decapoda, Palaemonidae) |
title_full_unstemmed |
Effects of ammonia stress in the Amazon river shrimp Macrobrachium amazonicum (Decapoda, Palaemonidae) |
title_sort |
Effects of ammonia stress in the Amazon river shrimp Macrobrachium amazonicum (Decapoda, Palaemonidae) |
author |
Pinto, Marcelo R. |
author_facet |
Pinto, Marcelo R. Lucena, Malson N. Faleiros, Rogerio Oliveira Almeida, Eduardo Alves [UNESP] McNamara, John C. Leone, Francisco A. |
author_role |
author |
author2 |
Lucena, Malson N. Faleiros, Rogerio Oliveira Almeida, Eduardo Alves [UNESP] McNamara, John C. Leone, Francisco A. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Pinto, Marcelo R. Lucena, Malson N. Faleiros, Rogerio Oliveira Almeida, Eduardo Alves [UNESP] McNamara, John C. Leone, Francisco A. |
dc.subject.por.fl_str_mv |
Ammonia exposure Gill microsome (Na+, K+)-ATPase V(H+)-ATPase Gene expression Oxidative enzymes Macrobrachium amazonicum |
topic |
Ammonia exposure Gill microsome (Na+, K+)-ATPase V(H+)-ATPase Gene expression Oxidative enzymes Macrobrachium amazonicum |
description |
We evaluate the effects of total ammonia nitrogen-N (TAN) exposure for 72 h on (Na+,K+)- and V(H+)-ATPase activities and on their subunit expressions in gills of the diadromous freshwater shrimp Macrobrachium amazonicum. Specific (Na+,K+)- and V(H+)-ATPase activities increased roughly 1.5- to 2 fold, respectively, after exposure to 2.0 mmol L-1 TAN. Quantitative RT-PCR analyses revealed a 2.5-fold increase in V(H+)-ATPase B subunit mRNA expression while (Na+,K+)-ATPase alpha-subunit expression was unchanged. Immunohistochemical analyses of the gill lamellae located the (Na+,K+)-ATPase throughout the intralamellar septal cells, independently of TAN concentration, while the V(H+)-ATPase was located in both the apical pillar cell flanges and pillar cell bodies. Systemic stress parameters like total hemocyte count decreased by 30% after exposure to 2.0 mmol L-1 TAN, accompanied by increased activities of the oxidative stress enzymes superoxide dismutase, glutathione reductase and glucose-6-phosphate dehydrogenase in the gills. The stress responses of M. amazonicum to elevated TAN include increases in gill (Na+,K+)- and V(H+)-ATPase activities that are accompanied by changes in oxidative stress enzyme activities, immune system effects and an increase in gill V(H+)-ATPase gene expression. These findings likely underpin physiological effects in a crustacean like M. amazonicum that exploits multiple ecosystems during its life cycle, as well as under culture conditions that may significantly impact shrimp production by the aquaculture industry. (C) 2015 Elsevier B.V. All rights reserved. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-01-01 2018-11-26T15:28:35Z 2018-11-26T15:28:35Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.aquatox.2015.10.021 Aquatic Toxicology. Amsterdam: Elsevier Science Bv, v. 170, p. 13-23, 2016. 0166-445X http://hdl.handle.net/11449/158678 10.1016/j.aquatox.2015.10.021 WOS:000368564500002 WOS000368564500002.pdf |
url |
http://dx.doi.org/10.1016/j.aquatox.2015.10.021 http://hdl.handle.net/11449/158678 |
identifier_str_mv |
Aquatic Toxicology. Amsterdam: Elsevier Science Bv, v. 170, p. 13-23, 2016. 0166-445X 10.1016/j.aquatox.2015.10.021 WOS:000368564500002 WOS000368564500002.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Aquatic Toxicology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
13-23 application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1797790129714102272 |