The herbicides trifluralin and tebuthiuron have no genotoxic or mutagenic potential as evidenced by genetic tests
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s11356-017-9966-5 http://hdl.handle.net/11449/170100 |
Resumo: | Brazil has been the largest world consumer of pesticides since 2008, followed by the USA. The herbicides trifluralin and tebuthiuron have been widely applied in agriculture. These herbicides are selective for some plant species, and their use brings various benefits. However, the genotoxic and mutagenic effects of tebuthiuron on non-target organisms are poorly known, and in addition, the effects of trifluralin must be better investigated. Therefore, this study employed genetic tests including the comet assay and micronucleus test to evaluate the genotoxic effects of trifluralin and tebuthiuron on HepG2 cells. In addition, we have used the Ames test to assess the mutagenic effects of the herbicides on the TA97a, TA98, TA100, and TA1535 strains of Salmonella typhimurium. On the basis of the comet assay and the micronucleus test, trifluralin did not cause genetic damage to HepG2 cells. In addition, trifluralin did not impact the tested S. typhimurium strains. Regarding tebuthiuron, literature has shown that this herbicide damaged DNA in Oreochromis niloticus. Nevertheless, we have found that tebuthiuron was not genotoxic to either HepG2 cells or the S. typhimurium strains. Therefore, neither trifluralin nor tebuthiuron exerted genotoxic or mutagenic potential at the tested conditions. |
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The herbicides trifluralin and tebuthiuron have no genotoxic or mutagenic potential as evidenced by genetic testsAmes testComet assayHepG2MicronucleusSalmonella typhimuriumBrazil has been the largest world consumer of pesticides since 2008, followed by the USA. The herbicides trifluralin and tebuthiuron have been widely applied in agriculture. These herbicides are selective for some plant species, and their use brings various benefits. However, the genotoxic and mutagenic effects of tebuthiuron on non-target organisms are poorly known, and in addition, the effects of trifluralin must be better investigated. Therefore, this study employed genetic tests including the comet assay and micronucleus test to evaluate the genotoxic effects of trifluralin and tebuthiuron on HepG2 cells. In addition, we have used the Ames test to assess the mutagenic effects of the herbicides on the TA97a, TA98, TA100, and TA1535 strains of Salmonella typhimurium. On the basis of the comet assay and the micronucleus test, trifluralin did not cause genetic damage to HepG2 cells. In addition, trifluralin did not impact the tested S. typhimurium strains. Regarding tebuthiuron, literature has shown that this herbicide damaged DNA in Oreochromis niloticus. Nevertheless, we have found that tebuthiuron was not genotoxic to either HepG2 cells or the S. typhimurium strains. Therefore, neither trifluralin nor tebuthiuron exerted genotoxic or mutagenic potential at the tested conditions.School of Pharmaceutical Sciences of Ribeirão Preto University of São Paulo, Av. do café, s/nFaculdade de Ciências Agronômicas Fazenda Experimental de Lageado Universidade Estadual Paulista “Júlio de Mesquita Filho”Faculdade de Filosofia Ciências e Letras de Ribeirão Preto (FFCLRP – USP), Av. Bandeirantes, 3900National Institute for Alternative Technologies of Detection Toxicological Evaluation and Removal of Micropollutants and Radioactives (INCT-DATREM) Unesp Institute of Chemistry, P.O. Box 355Faculdade de Ciências Agronômicas Fazenda Experimental de Lageado Universidade Estadual Paulista “Júlio de Mesquita Filho”National Institute for Alternative Technologies of Detection Toxicological Evaluation and Removal of Micropollutants and Radioactives (INCT-DATREM) Unesp Institute of Chemistry, P.O. Box 355Universidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Franco-Bernardes, Mariana FurioRocha, Otávio PelegrinoPereira, Lilian Cristina [UNESP]Tasso, Maria JúliaMeireles, Gabrielade Oliveira, Danielle Palma [UNESP]Dorta, Daniel Junqueira [UNESP]2018-12-11T16:49:16Z2018-12-11T16:49:16Z2017-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article24029-24037application/pdfhttp://dx.doi.org/10.1007/s11356-017-9966-5Environmental Science and Pollution Research, v. 24, n. 30, p. 24029-24037, 2017.1614-74990944-1344http://hdl.handle.net/11449/17010010.1007/s11356-017-9966-52-s2.0-850288042322-s2.0-85028804232.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEnvironmental Science and Pollution Research0,858info:eu-repo/semantics/openAccess2023-11-21T06:11:32Zoai:repositorio.unesp.br:11449/170100Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:20:00.614541Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
The herbicides trifluralin and tebuthiuron have no genotoxic or mutagenic potential as evidenced by genetic tests |
title |
The herbicides trifluralin and tebuthiuron have no genotoxic or mutagenic potential as evidenced by genetic tests |
spellingShingle |
The herbicides trifluralin and tebuthiuron have no genotoxic or mutagenic potential as evidenced by genetic tests Franco-Bernardes, Mariana Furio Ames test Comet assay HepG2 Micronucleus Salmonella typhimurium |
title_short |
The herbicides trifluralin and tebuthiuron have no genotoxic or mutagenic potential as evidenced by genetic tests |
title_full |
The herbicides trifluralin and tebuthiuron have no genotoxic or mutagenic potential as evidenced by genetic tests |
title_fullStr |
The herbicides trifluralin and tebuthiuron have no genotoxic or mutagenic potential as evidenced by genetic tests |
title_full_unstemmed |
The herbicides trifluralin and tebuthiuron have no genotoxic or mutagenic potential as evidenced by genetic tests |
title_sort |
The herbicides trifluralin and tebuthiuron have no genotoxic or mutagenic potential as evidenced by genetic tests |
author |
Franco-Bernardes, Mariana Furio |
author_facet |
Franco-Bernardes, Mariana Furio Rocha, Otávio Pelegrino Pereira, Lilian Cristina [UNESP] Tasso, Maria Júlia Meireles, Gabriela de Oliveira, Danielle Palma [UNESP] Dorta, Daniel Junqueira [UNESP] |
author_role |
author |
author2 |
Rocha, Otávio Pelegrino Pereira, Lilian Cristina [UNESP] Tasso, Maria Júlia Meireles, Gabriela de Oliveira, Danielle Palma [UNESP] Dorta, Daniel Junqueira [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Franco-Bernardes, Mariana Furio Rocha, Otávio Pelegrino Pereira, Lilian Cristina [UNESP] Tasso, Maria Júlia Meireles, Gabriela de Oliveira, Danielle Palma [UNESP] Dorta, Daniel Junqueira [UNESP] |
dc.subject.por.fl_str_mv |
Ames test Comet assay HepG2 Micronucleus Salmonella typhimurium |
topic |
Ames test Comet assay HepG2 Micronucleus Salmonella typhimurium |
description |
Brazil has been the largest world consumer of pesticides since 2008, followed by the USA. The herbicides trifluralin and tebuthiuron have been widely applied in agriculture. These herbicides are selective for some plant species, and their use brings various benefits. However, the genotoxic and mutagenic effects of tebuthiuron on non-target organisms are poorly known, and in addition, the effects of trifluralin must be better investigated. Therefore, this study employed genetic tests including the comet assay and micronucleus test to evaluate the genotoxic effects of trifluralin and tebuthiuron on HepG2 cells. In addition, we have used the Ames test to assess the mutagenic effects of the herbicides on the TA97a, TA98, TA100, and TA1535 strains of Salmonella typhimurium. On the basis of the comet assay and the micronucleus test, trifluralin did not cause genetic damage to HepG2 cells. In addition, trifluralin did not impact the tested S. typhimurium strains. Regarding tebuthiuron, literature has shown that this herbicide damaged DNA in Oreochromis niloticus. Nevertheless, we have found that tebuthiuron was not genotoxic to either HepG2 cells or the S. typhimurium strains. Therefore, neither trifluralin nor tebuthiuron exerted genotoxic or mutagenic potential at the tested conditions. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-10-01 2018-12-11T16:49:16Z 2018-12-11T16:49:16Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s11356-017-9966-5 Environmental Science and Pollution Research, v. 24, n. 30, p. 24029-24037, 2017. 1614-7499 0944-1344 http://hdl.handle.net/11449/170100 10.1007/s11356-017-9966-5 2-s2.0-85028804232 2-s2.0-85028804232.pdf |
url |
http://dx.doi.org/10.1007/s11356-017-9966-5 http://hdl.handle.net/11449/170100 |
identifier_str_mv |
Environmental Science and Pollution Research, v. 24, n. 30, p. 24029-24037, 2017. 1614-7499 0944-1344 10.1007/s11356-017-9966-5 2-s2.0-85028804232 2-s2.0-85028804232.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Environmental Science and Pollution Research 0,858 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
24029-24037 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128920970592256 |