HPLC-PDA and UPLC-ESI-TqD-MS/MS chromatographic analyses of commercial samples of Camellia sinensis (L.) Kuntze (“green tea”)

Detalhes bibliográficos
Autor(a) principal: Silva, J. Z.
Data de Publicação: 2016
Outros Autores: Tangerina, M. M.P. [UNESP], Rocha, C. Q. [UNESP], Amaral, F. P., Bento, C. C., Tahira, L. S. [UNESP], Vilegas, W. [UNESP], Sannomiya, M.
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/1983-084X/0115
http://hdl.handle.net/11449/249665
Resumo: The analysis by HPLC-PDA, PCA and the injection of standard compounds contributed considerably for the evaluation of the secondary metabolites of four commercial samples of Camellia sinensis. All analyzed samples, except sample number 2, when extracted with ethanol-water mixture, presented catechin derivatives, fenolic acids, methylxanthines and flavonoids, which are commonly found in the species under study. It is possible that sample number 2 does not correspond to C. sinensis or may have been collected in a specific time when the main metabolites were not being produced. Quantification by UPLC-ESI-TqD-MS/ MS of the caffeine content the samples of C. sinensis showed that the values obtained in this work vary between 2.72 to 7.02 g/100 g of crude extract. Moreover, all samples analyzed showed a higher content of caffeine than those previously reported in the literature, except sample number 1. Analysis by PCA of the total ions of the samples showed that sample number 3 infusion is quite different from the other samples. These data allow us to infer that caffeine can be a taxonomic marker in the samples of C. sinensis. However, its presence cannot be conclusive in the quality control of a medicinal plant, since the total composition of caffeine may vary significantly and, therefore, and its consumption may not provide the expected efficacy.
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spelling HPLC-PDA and UPLC-ESI-TqD-MS/MS chromatographic analyses of commercial samples of Camellia sinensis (L.) Kuntze (“green tea”)Análises cromatográficas por HPLC-PDA e UPLC–ESI-TqD-MS/MS de amostras comerciais de Camellia sinensis (L.) Kuntze (“chá verde”)Camellia sinensisHPLC-PDATLCUPLC-ESI-TqD-MS/MSThe analysis by HPLC-PDA, PCA and the injection of standard compounds contributed considerably for the evaluation of the secondary metabolites of four commercial samples of Camellia sinensis. All analyzed samples, except sample number 2, when extracted with ethanol-water mixture, presented catechin derivatives, fenolic acids, methylxanthines and flavonoids, which are commonly found in the species under study. It is possible that sample number 2 does not correspond to C. sinensis or may have been collected in a specific time when the main metabolites were not being produced. Quantification by UPLC-ESI-TqD-MS/ MS of the caffeine content the samples of C. sinensis showed that the values obtained in this work vary between 2.72 to 7.02 g/100 g of crude extract. Moreover, all samples analyzed showed a higher content of caffeine than those previously reported in the literature, except sample number 1. Analysis by PCA of the total ions of the samples showed that sample number 3 infusion is quite different from the other samples. These data allow us to infer that caffeine can be a taxonomic marker in the samples of C. sinensis. However, its presence cannot be conclusive in the quality control of a medicinal plant, since the total composition of caffeine may vary significantly and, therefore, and its consumption may not provide the expected efficacy.Universidade de São Paulo School of Arts Science and Humanities, SPUniversidade Estadual Paulista “Júlio de Mesquita Filho”, Campus of São Paulo CoastUniversidade Federal de Rondônia, Campus de Rolim de MouraUniversidade Estadual Paulista “Júlio de Mesquita Filho”, Campus of São Paulo CoastUniversidade de São Paulo (USP)Universidade Estadual Paulista (UNESP)Universidade Federal de RondôniaSilva, J. Z.Tangerina, M. M.P. [UNESP]Rocha, C. Q. [UNESP]Amaral, F. P.Bento, C. C.Tahira, L. S. [UNESP]Vilegas, W. [UNESP]Sannomiya, M.2023-07-29T16:05:56Z2023-07-29T16:05:56Z2016-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article636-643http://dx.doi.org/10.1590/1983-084X/0115Revista Brasileira de Plantas Medicinais, v. 18, n. 3, p. 636-643, 2016.1983-084X1516-0572http://hdl.handle.net/11449/24966510.1590/1983-084X/01152-s2.0-85148291983Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporRevista Brasileira de Plantas Medicinaisinfo:eu-repo/semantics/openAccess2023-07-29T16:05:56Zoai:repositorio.unesp.br:11449/249665Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:00:15.677293Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv HPLC-PDA and UPLC-ESI-TqD-MS/MS chromatographic analyses of commercial samples of Camellia sinensis (L.) Kuntze (“green tea”)
Análises cromatográficas por HPLC-PDA e UPLC–ESI-TqD-MS/MS de amostras comerciais de Camellia sinensis (L.) Kuntze (“chá verde”)
title HPLC-PDA and UPLC-ESI-TqD-MS/MS chromatographic analyses of commercial samples of Camellia sinensis (L.) Kuntze (“green tea”)
spellingShingle HPLC-PDA and UPLC-ESI-TqD-MS/MS chromatographic analyses of commercial samples of Camellia sinensis (L.) Kuntze (“green tea”)
Silva, J. Z.
Camellia sinensis
HPLC-PDA
TLC
UPLC-ESI-TqD-MS/MS
title_short HPLC-PDA and UPLC-ESI-TqD-MS/MS chromatographic analyses of commercial samples of Camellia sinensis (L.) Kuntze (“green tea”)
title_full HPLC-PDA and UPLC-ESI-TqD-MS/MS chromatographic analyses of commercial samples of Camellia sinensis (L.) Kuntze (“green tea”)
title_fullStr HPLC-PDA and UPLC-ESI-TqD-MS/MS chromatographic analyses of commercial samples of Camellia sinensis (L.) Kuntze (“green tea”)
title_full_unstemmed HPLC-PDA and UPLC-ESI-TqD-MS/MS chromatographic analyses of commercial samples of Camellia sinensis (L.) Kuntze (“green tea”)
title_sort HPLC-PDA and UPLC-ESI-TqD-MS/MS chromatographic analyses of commercial samples of Camellia sinensis (L.) Kuntze (“green tea”)
author Silva, J. Z.
author_facet Silva, J. Z.
Tangerina, M. M.P. [UNESP]
Rocha, C. Q. [UNESP]
Amaral, F. P.
Bento, C. C.
Tahira, L. S. [UNESP]
Vilegas, W. [UNESP]
Sannomiya, M.
author_role author
author2 Tangerina, M. M.P. [UNESP]
Rocha, C. Q. [UNESP]
Amaral, F. P.
Bento, C. C.
Tahira, L. S. [UNESP]
Vilegas, W. [UNESP]
Sannomiya, M.
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
Universidade Estadual Paulista (UNESP)
Universidade Federal de Rondônia
dc.contributor.author.fl_str_mv Silva, J. Z.
Tangerina, M. M.P. [UNESP]
Rocha, C. Q. [UNESP]
Amaral, F. P.
Bento, C. C.
Tahira, L. S. [UNESP]
Vilegas, W. [UNESP]
Sannomiya, M.
dc.subject.por.fl_str_mv Camellia sinensis
HPLC-PDA
TLC
UPLC-ESI-TqD-MS/MS
topic Camellia sinensis
HPLC-PDA
TLC
UPLC-ESI-TqD-MS/MS
description The analysis by HPLC-PDA, PCA and the injection of standard compounds contributed considerably for the evaluation of the secondary metabolites of four commercial samples of Camellia sinensis. All analyzed samples, except sample number 2, when extracted with ethanol-water mixture, presented catechin derivatives, fenolic acids, methylxanthines and flavonoids, which are commonly found in the species under study. It is possible that sample number 2 does not correspond to C. sinensis or may have been collected in a specific time when the main metabolites were not being produced. Quantification by UPLC-ESI-TqD-MS/ MS of the caffeine content the samples of C. sinensis showed that the values obtained in this work vary between 2.72 to 7.02 g/100 g of crude extract. Moreover, all samples analyzed showed a higher content of caffeine than those previously reported in the literature, except sample number 1. Analysis by PCA of the total ions of the samples showed that sample number 3 infusion is quite different from the other samples. These data allow us to infer that caffeine can be a taxonomic marker in the samples of C. sinensis. However, its presence cannot be conclusive in the quality control of a medicinal plant, since the total composition of caffeine may vary significantly and, therefore, and its consumption may not provide the expected efficacy.
publishDate 2016
dc.date.none.fl_str_mv 2016-01-01
2023-07-29T16:05:56Z
2023-07-29T16:05:56Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/1983-084X/0115
Revista Brasileira de Plantas Medicinais, v. 18, n. 3, p. 636-643, 2016.
1983-084X
1516-0572
http://hdl.handle.net/11449/249665
10.1590/1983-084X/0115
2-s2.0-85148291983
url http://dx.doi.org/10.1590/1983-084X/0115
http://hdl.handle.net/11449/249665
identifier_str_mv Revista Brasileira de Plantas Medicinais, v. 18, n. 3, p. 636-643, 2016.
1983-084X
1516-0572
10.1590/1983-084X/0115
2-s2.0-85148291983
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv Revista Brasileira de Plantas Medicinais
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 636-643
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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