Biocompatibility, induction of mineralization and antimicrobial activity of experimental intracanal pastes based on glass and glass-ceramic materials
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1111/iej.13382 http://hdl.handle.net/11449/199323 |
Resumo: | Aim: To evaluate the biocompatibility, induction of mineralization and antimicrobial activity of experimental intracanal pastes based on two glass and glass-ceramic materials. Calcium hydroxide (Ca(OH)2) paste was used as the positive control. Methodology: The glass-ceramic powder [two-phased Biosilicate (BS-2P)] and F18 bioactive glass were mixed with distilled water (ratio 2 : 1), inserted in polyethylene tubes and implanted in the subcutaneous tissues of 16 rats. Empty tubes were used as negative control. After 7 and 30 days (n = 8), the rats were euthanized for haematoxylin–eosin, von Kossa, polarized light and osteopontin (OPN) immunolabeling analysis. Direct contact tests using a suspension of each paste were performed with Enterococcus faecalis planktonic cells to evaluate antimicrobial activity (24 h of contact), in a pilot study. The number of CFU mL−1 was calculated for each group. The antimicrobial analysis data were submitted to one-way anova and Tukey tests, whilst biocompatibility and immunohistochemical data were submitted to the Kruskal–Wallis and Dunn tests (P < 0.05). Results: Most specimens of the control, BS-2P and Ca(OH)2 groups were associated with moderate inflammation seven days following implantation, whilst F18 was associated with moderate to severe inflammation, without differences amongst the groups (P > 0.05). At 30 days, most specimens of control, F18 and BS-2P groups had mild inflammation, whilst Ca(OH)2 had mild to moderate inflammation; however, no differences were determined amongst the groups (P > 0.05). The fibrous capsule was thick at 7 days, becoming thin at 30 days. All pastes induced von Kossa-positive structures and were birefringent to polarized light. At seven days, the BS-2P group had significantly more OPN immunolabeling compared to the control and Ca(OH)2 groups (P < 0.05). At 30 days, the F18 group had significantly more OPN immunolabeling compared to the control and Ca(OH)2 groups (P < 0.05). All pastes reduced the total number of E. faecalis; however, the reduction was only significant when comparing BS-2P and Ca(OH)2 groups to the control (P < 0.05). Only calcium hydroxide eliminated E. faecalis. Conclusions: Experimental BS-2P and F18 pastes were biocompatible, stimulated biomineralization and induced significant OPN immunolabeling compared to Ca(OH)2. Only the BS-2P paste demonstrated antimicrobial activity comparable to Ca(OH)2. |
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Repositório Institucional da UNESP |
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Biocompatibility, induction of mineralization and antimicrobial activity of experimental intracanal pastes based on glass and glass-ceramic materialsbioactive glassbioceramicsbiocompatibilitybiomineralizationosteopontinAim: To evaluate the biocompatibility, induction of mineralization and antimicrobial activity of experimental intracanal pastes based on two glass and glass-ceramic materials. Calcium hydroxide (Ca(OH)2) paste was used as the positive control. Methodology: The glass-ceramic powder [two-phased Biosilicate (BS-2P)] and F18 bioactive glass were mixed with distilled water (ratio 2 : 1), inserted in polyethylene tubes and implanted in the subcutaneous tissues of 16 rats. Empty tubes were used as negative control. After 7 and 30 days (n = 8), the rats were euthanized for haematoxylin–eosin, von Kossa, polarized light and osteopontin (OPN) immunolabeling analysis. Direct contact tests using a suspension of each paste were performed with Enterococcus faecalis planktonic cells to evaluate antimicrobial activity (24 h of contact), in a pilot study. The number of CFU mL−1 was calculated for each group. The antimicrobial analysis data were submitted to one-way anova and Tukey tests, whilst biocompatibility and immunohistochemical data were submitted to the Kruskal–Wallis and Dunn tests (P < 0.05). Results: Most specimens of the control, BS-2P and Ca(OH)2 groups were associated with moderate inflammation seven days following implantation, whilst F18 was associated with moderate to severe inflammation, without differences amongst the groups (P > 0.05). At 30 days, most specimens of control, F18 and BS-2P groups had mild inflammation, whilst Ca(OH)2 had mild to moderate inflammation; however, no differences were determined amongst the groups (P > 0.05). The fibrous capsule was thick at 7 days, becoming thin at 30 days. All pastes induced von Kossa-positive structures and were birefringent to polarized light. At seven days, the BS-2P group had significantly more OPN immunolabeling compared to the control and Ca(OH)2 groups (P < 0.05). At 30 days, the F18 group had significantly more OPN immunolabeling compared to the control and Ca(OH)2 groups (P < 0.05). All pastes reduced the total number of E. faecalis; however, the reduction was only significant when comparing BS-2P and Ca(OH)2 groups to the control (P < 0.05). Only calcium hydroxide eliminated E. faecalis. Conclusions: Experimental BS-2P and F18 pastes were biocompatible, stimulated biomineralization and induced significant OPN immunolabeling compared to Ca(OH)2. Only the BS-2P paste demonstrated antimicrobial activity comparable to Ca(OH)2.Endodontics Section Department of Preventive and Restorative Dentistry School of Dentistry São Paulo State University (Unesp)Endodontic Section Department of Restorative Dentistry School of Dentistry Universidade Federal de Minas GeraisVitreous Materials Laboratory (LaMaV) Department of Materials Engineering Federal University of São Carlos (UFSCar)Department of Basic Science School of Dentistry São Paulo State University (Unesp)Endodontics Section Department of Preventive and Restorative Dentistry School of Dentistry São Paulo State University (Unesp)Department of Basic Science School of Dentistry São Paulo State University (Unesp)Universidade Estadual Paulista (Unesp)Universidade Federal de Minas Gerais (UFMG)Universidade Federal de São Carlos (UFSCar)de Araújo Lopes, J. M. [UNESP]Benetti, F. [UNESP]Rezende, G. C. [UNESP]Souza, M. T.Conti, L. C. [UNESP]Ervolino, E. [UNESP]Jacinto, R. C. [UNESP]Zanotto, E. D.Cintra, L. T.A. [UNESP]2020-12-12T01:36:39Z2020-12-12T01:36:39Z2020-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1111/iej.13382International Endodontic Journal.1365-25910143-2885http://hdl.handle.net/11449/19932310.1111/iej.133822-s2.0-85090123199Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Endodontic Journalinfo:eu-repo/semantics/openAccess2021-10-23T07:07:39Zoai:repositorio.unesp.br:11449/199323Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T07:07:39Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Biocompatibility, induction of mineralization and antimicrobial activity of experimental intracanal pastes based on glass and glass-ceramic materials |
title |
Biocompatibility, induction of mineralization and antimicrobial activity of experimental intracanal pastes based on glass and glass-ceramic materials |
spellingShingle |
Biocompatibility, induction of mineralization and antimicrobial activity of experimental intracanal pastes based on glass and glass-ceramic materials de Araújo Lopes, J. M. [UNESP] bioactive glass bioceramics biocompatibility biomineralization osteopontin |
title_short |
Biocompatibility, induction of mineralization and antimicrobial activity of experimental intracanal pastes based on glass and glass-ceramic materials |
title_full |
Biocompatibility, induction of mineralization and antimicrobial activity of experimental intracanal pastes based on glass and glass-ceramic materials |
title_fullStr |
Biocompatibility, induction of mineralization and antimicrobial activity of experimental intracanal pastes based on glass and glass-ceramic materials |
title_full_unstemmed |
Biocompatibility, induction of mineralization and antimicrobial activity of experimental intracanal pastes based on glass and glass-ceramic materials |
title_sort |
Biocompatibility, induction of mineralization and antimicrobial activity of experimental intracanal pastes based on glass and glass-ceramic materials |
author |
de Araújo Lopes, J. M. [UNESP] |
author_facet |
de Araújo Lopes, J. M. [UNESP] Benetti, F. [UNESP] Rezende, G. C. [UNESP] Souza, M. T. Conti, L. C. [UNESP] Ervolino, E. [UNESP] Jacinto, R. C. [UNESP] Zanotto, E. D. Cintra, L. T.A. [UNESP] |
author_role |
author |
author2 |
Benetti, F. [UNESP] Rezende, G. C. [UNESP] Souza, M. T. Conti, L. C. [UNESP] Ervolino, E. [UNESP] Jacinto, R. C. [UNESP] Zanotto, E. D. Cintra, L. T.A. [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Federal de Minas Gerais (UFMG) Universidade Federal de São Carlos (UFSCar) |
dc.contributor.author.fl_str_mv |
de Araújo Lopes, J. M. [UNESP] Benetti, F. [UNESP] Rezende, G. C. [UNESP] Souza, M. T. Conti, L. C. [UNESP] Ervolino, E. [UNESP] Jacinto, R. C. [UNESP] Zanotto, E. D. Cintra, L. T.A. [UNESP] |
dc.subject.por.fl_str_mv |
bioactive glass bioceramics biocompatibility biomineralization osteopontin |
topic |
bioactive glass bioceramics biocompatibility biomineralization osteopontin |
description |
Aim: To evaluate the biocompatibility, induction of mineralization and antimicrobial activity of experimental intracanal pastes based on two glass and glass-ceramic materials. Calcium hydroxide (Ca(OH)2) paste was used as the positive control. Methodology: The glass-ceramic powder [two-phased Biosilicate (BS-2P)] and F18 bioactive glass were mixed with distilled water (ratio 2 : 1), inserted in polyethylene tubes and implanted in the subcutaneous tissues of 16 rats. Empty tubes were used as negative control. After 7 and 30 days (n = 8), the rats were euthanized for haematoxylin–eosin, von Kossa, polarized light and osteopontin (OPN) immunolabeling analysis. Direct contact tests using a suspension of each paste were performed with Enterococcus faecalis planktonic cells to evaluate antimicrobial activity (24 h of contact), in a pilot study. The number of CFU mL−1 was calculated for each group. The antimicrobial analysis data were submitted to one-way anova and Tukey tests, whilst biocompatibility and immunohistochemical data were submitted to the Kruskal–Wallis and Dunn tests (P < 0.05). Results: Most specimens of the control, BS-2P and Ca(OH)2 groups were associated with moderate inflammation seven days following implantation, whilst F18 was associated with moderate to severe inflammation, without differences amongst the groups (P > 0.05). At 30 days, most specimens of control, F18 and BS-2P groups had mild inflammation, whilst Ca(OH)2 had mild to moderate inflammation; however, no differences were determined amongst the groups (P > 0.05). The fibrous capsule was thick at 7 days, becoming thin at 30 days. All pastes induced von Kossa-positive structures and were birefringent to polarized light. At seven days, the BS-2P group had significantly more OPN immunolabeling compared to the control and Ca(OH)2 groups (P < 0.05). At 30 days, the F18 group had significantly more OPN immunolabeling compared to the control and Ca(OH)2 groups (P < 0.05). All pastes reduced the total number of E. faecalis; however, the reduction was only significant when comparing BS-2P and Ca(OH)2 groups to the control (P < 0.05). Only calcium hydroxide eliminated E. faecalis. Conclusions: Experimental BS-2P and F18 pastes were biocompatible, stimulated biomineralization and induced significant OPN immunolabeling compared to Ca(OH)2. Only the BS-2P paste demonstrated antimicrobial activity comparable to Ca(OH)2. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T01:36:39Z 2020-12-12T01:36:39Z 2020-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1111/iej.13382 International Endodontic Journal. 1365-2591 0143-2885 http://hdl.handle.net/11449/199323 10.1111/iej.13382 2-s2.0-85090123199 |
url |
http://dx.doi.org/10.1111/iej.13382 http://hdl.handle.net/11449/199323 |
identifier_str_mv |
International Endodontic Journal. 1365-2591 0143-2885 10.1111/iej.13382 2-s2.0-85090123199 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
International Endodontic Journal |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965725904863232 |