Possible mechanism by which zinc protects the testicular function of rats exposed to cigarette smoke
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/S1734-1140(12)70951-9 http://hdl.handle.net/11449/195765 |
Resumo: | Background: The aim of this study was to evaluate the changes in testicular function of rats due to cigarette smoke exposure and the possible mechanism by which zinc protects against these alterations. Methods: Male Wistar rats (60 days old) were randomly divided into 3 groups: control (G1, n = 10); exposed to cigarette smoke (G2, n = 10; 20 cigarettes/day/9 weeks) and exposed to cigarette smoke and supplemented with zinc (G3, n = 8; 20 cigarettes/day/9 weeks; 20 mg/kg zinc chloride daily for 9 weeks, by gavage). After the treatment period, the animals were euthanized, and materials were collected for analyses. Results: G2 rats showed a reduction in body mass; impaired sperm concentration, motility, morphology and vitality; and increased malonaldehyde and thiol group levels and superoxide dismutase activity as compared to G1. Zinc prevented the reduction of sperm concentration and the excessive increase of lipid peroxidation and induced an increase in plasma testosterone levels, wet weight of testis and thiol group concentration. Conclusions: Exposure to cigarette smoke led to harmful effects on testicular function at least partially due to the exacerbation of oxidative stress. Supplementary zinc had an important modulator/protector effect on certain parameters. The mechanism of zinc protection can be through an increase of SH concentration. Thus, zinc supplementation may be a promising addition to conventional treatments for male infertility related to smoking. |
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Possible mechanism by which zinc protects the testicular function of rats exposed to cigarette smokeoxidative stressratssemensmokingspermatozoatestiszincBackground: The aim of this study was to evaluate the changes in testicular function of rats due to cigarette smoke exposure and the possible mechanism by which zinc protects against these alterations. Methods: Male Wistar rats (60 days old) were randomly divided into 3 groups: control (G1, n = 10); exposed to cigarette smoke (G2, n = 10; 20 cigarettes/day/9 weeks) and exposed to cigarette smoke and supplemented with zinc (G3, n = 8; 20 cigarettes/day/9 weeks; 20 mg/kg zinc chloride daily for 9 weeks, by gavage). After the treatment period, the animals were euthanized, and materials were collected for analyses. Results: G2 rats showed a reduction in body mass; impaired sperm concentration, motility, morphology and vitality; and increased malonaldehyde and thiol group levels and superoxide dismutase activity as compared to G1. Zinc prevented the reduction of sperm concentration and the excessive increase of lipid peroxidation and induced an increase in plasma testosterone levels, wet weight of testis and thiol group concentration. Conclusions: Exposure to cigarette smoke led to harmful effects on testicular function at least partially due to the exacerbation of oxidative stress. Supplementary zinc had an important modulator/protector effect on certain parameters. The mechanism of zinc protection can be through an increase of SH concentration. Thus, zinc supplementation may be a promising addition to conventional treatments for male infertility related to smoking.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)UNESP Univ Estadual Paulista, Botucatu Med Sch, Inst Biosci, Dept Pharmacol, BR-18618970 Sao Paulo, BrazilUNESP Univ Estadual Paulista, Botucatu Med Sch, Dept Gynecol & Obstet, BR-18618970 Sao Paulo, BrazilUNESP Univ Estadual Paulista, Botucatu Med Sch, Inst Biosci, Dept Pharmacol, BR-18618970 Sao Paulo, BrazilUNESP Univ Estadual Paulista, Botucatu Med Sch, Dept Gynecol & Obstet, BR-18618970 Sao Paulo, BrazilFAPESP: 2009/03475-4Polish Acad Sciences Inst PharmacologyUniversidade Estadual Paulista (Unesp)Sankako, Michele K. [UNESP]Garcia, Patricia C. [UNESP]Piffer, Renata C. [UNESP]Dallaqua, Bruna [UNESP]Damasceno, Debora C. [UNESP]Pereira, Oduvaldo C. M. [UNESP]2020-12-10T18:02:44Z2020-12-10T18:02:44Z2012-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1537-1546http://dx.doi.org/10.1016/S1734-1140(12)70951-9Pharmacological Reports. Krakow: Polish Acad Sciences Inst Pharmacology, v. 64, n. 6, p. 1537-1546, 2012.1734-1140http://hdl.handle.net/11449/19576510.1016/S1734-1140(12)70951-9WOS:000208844000026Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPharmacological Reportsinfo:eu-repo/semantics/openAccess2024-08-16T14:13:02Zoai:repositorio.unesp.br:11449/195765Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-16T14:13:02Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Possible mechanism by which zinc protects the testicular function of rats exposed to cigarette smoke |
title |
Possible mechanism by which zinc protects the testicular function of rats exposed to cigarette smoke |
spellingShingle |
Possible mechanism by which zinc protects the testicular function of rats exposed to cigarette smoke Sankako, Michele K. [UNESP] oxidative stress rats semen smoking spermatozoa testis zinc |
title_short |
Possible mechanism by which zinc protects the testicular function of rats exposed to cigarette smoke |
title_full |
Possible mechanism by which zinc protects the testicular function of rats exposed to cigarette smoke |
title_fullStr |
Possible mechanism by which zinc protects the testicular function of rats exposed to cigarette smoke |
title_full_unstemmed |
Possible mechanism by which zinc protects the testicular function of rats exposed to cigarette smoke |
title_sort |
Possible mechanism by which zinc protects the testicular function of rats exposed to cigarette smoke |
author |
Sankako, Michele K. [UNESP] |
author_facet |
Sankako, Michele K. [UNESP] Garcia, Patricia C. [UNESP] Piffer, Renata C. [UNESP] Dallaqua, Bruna [UNESP] Damasceno, Debora C. [UNESP] Pereira, Oduvaldo C. M. [UNESP] |
author_role |
author |
author2 |
Garcia, Patricia C. [UNESP] Piffer, Renata C. [UNESP] Dallaqua, Bruna [UNESP] Damasceno, Debora C. [UNESP] Pereira, Oduvaldo C. M. [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Sankako, Michele K. [UNESP] Garcia, Patricia C. [UNESP] Piffer, Renata C. [UNESP] Dallaqua, Bruna [UNESP] Damasceno, Debora C. [UNESP] Pereira, Oduvaldo C. M. [UNESP] |
dc.subject.por.fl_str_mv |
oxidative stress rats semen smoking spermatozoa testis zinc |
topic |
oxidative stress rats semen smoking spermatozoa testis zinc |
description |
Background: The aim of this study was to evaluate the changes in testicular function of rats due to cigarette smoke exposure and the possible mechanism by which zinc protects against these alterations. Methods: Male Wistar rats (60 days old) were randomly divided into 3 groups: control (G1, n = 10); exposed to cigarette smoke (G2, n = 10; 20 cigarettes/day/9 weeks) and exposed to cigarette smoke and supplemented with zinc (G3, n = 8; 20 cigarettes/day/9 weeks; 20 mg/kg zinc chloride daily for 9 weeks, by gavage). After the treatment period, the animals were euthanized, and materials were collected for analyses. Results: G2 rats showed a reduction in body mass; impaired sperm concentration, motility, morphology and vitality; and increased malonaldehyde and thiol group levels and superoxide dismutase activity as compared to G1. Zinc prevented the reduction of sperm concentration and the excessive increase of lipid peroxidation and induced an increase in plasma testosterone levels, wet weight of testis and thiol group concentration. Conclusions: Exposure to cigarette smoke led to harmful effects on testicular function at least partially due to the exacerbation of oxidative stress. Supplementary zinc had an important modulator/protector effect on certain parameters. The mechanism of zinc protection can be through an increase of SH concentration. Thus, zinc supplementation may be a promising addition to conventional treatments for male infertility related to smoking. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-11-01 2020-12-10T18:02:44Z 2020-12-10T18:02:44Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/S1734-1140(12)70951-9 Pharmacological Reports. Krakow: Polish Acad Sciences Inst Pharmacology, v. 64, n. 6, p. 1537-1546, 2012. 1734-1140 http://hdl.handle.net/11449/195765 10.1016/S1734-1140(12)70951-9 WOS:000208844000026 |
url |
http://dx.doi.org/10.1016/S1734-1140(12)70951-9 http://hdl.handle.net/11449/195765 |
identifier_str_mv |
Pharmacological Reports. Krakow: Polish Acad Sciences Inst Pharmacology, v. 64, n. 6, p. 1537-1546, 2012. 1734-1140 10.1016/S1734-1140(12)70951-9 WOS:000208844000026 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Pharmacological Reports |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1537-1546 |
dc.publisher.none.fl_str_mv |
Polish Acad Sciences Inst Pharmacology |
publisher.none.fl_str_mv |
Polish Acad Sciences Inst Pharmacology |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128211447447552 |