IL-6, TNF-α, and IL-10 levels/ polymorphisms and their association with type 2 diabetes mellitus and obesity in Brazilian individuals
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/2359-3997000000254 http://hdl.handle.net/11449/169701 |
Resumo: | Objective: This study aimed to investigate the association of plasma TNF-α, IL-6, and lL-10 levels and cytokine gene polymorphisms [TNF-α (-308 G→A), IL-6 (-174 C→G) and IL-10 (-1082 A→G, -819 T→C and -592 A→C)] in type 2 diabetes mellitus (T2DM) and obese patients. Subjects and methods: One hundred and two T2DM patients and 62 controls were included in this study. Cytokine plasma levels were measured by the Cytometric Bead Array method. Genotyping was carried out by the polymerase chain reaction. Results: IL-6 levels were significantly different between T2DM patients and controls. Interestingly, IL-6 levels were higher in T2DM patients with BMI > 30 kg/m2 compared with other patients and obese controls. The genotype and allele frequencies were similar between patients and controls. In the T2DM group, the SNP IL-10 -819 T/C showed a difference between the cytokine level and genotypes: IL-10 level in the TT genotype was significantly higher when compared to CC genotype. Conclusions: These results suggest an association between IL-6 levels and obesity, and IL-10 levels and the SNP -819 T/C in T2DM. Knowledge of these variants in T2DM might contribute to a better understanding of the role of inflammation in the etiology and progression of this disease. |
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IL-6, TNF-α, and IL-10 levels/ polymorphisms and their association with type 2 diabetes mellitus and obesity in Brazilian individualsInterleukin-10Interleukin-6PolymorphismsTumor necrosis factor-alphaType 2 diabetes mellitusObjective: This study aimed to investigate the association of plasma TNF-α, IL-6, and lL-10 levels and cytokine gene polymorphisms [TNF-α (-308 G→A), IL-6 (-174 C→G) and IL-10 (-1082 A→G, -819 T→C and -592 A→C)] in type 2 diabetes mellitus (T2DM) and obese patients. Subjects and methods: One hundred and two T2DM patients and 62 controls were included in this study. Cytokine plasma levels were measured by the Cytometric Bead Array method. Genotyping was carried out by the polymerase chain reaction. Results: IL-6 levels were significantly different between T2DM patients and controls. Interestingly, IL-6 levels were higher in T2DM patients with BMI > 30 kg/m2 compared with other patients and obese controls. The genotype and allele frequencies were similar between patients and controls. In the T2DM group, the SNP IL-10 -819 T/C showed a difference between the cytokine level and genotypes: IL-10 level in the TT genotype was significantly higher when compared to CC genotype. Conclusions: These results suggest an association between IL-6 levels and obesity, and IL-10 levels and the SNP -819 T/C in T2DM. Knowledge of these variants in T2DM might contribute to a better understanding of the role of inflammation in the etiology and progression of this disease.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)Universidade Federal de Minas GeraisInstituto de Ciências Biológicas Universidade Federal de Minas Gerais (UFMG)Instituto de Ensino e Pesquisa Santa Casa de Belo HorizonteFaculdade de Farmácia UFMGInstituto de Biociências Universidade Estadual Paulista Júlio de Mesquita Filho (Unesp)Instituto de Biociências Universidade Estadual Paulista Júlio de Mesquita Filho (Unesp)Universidade Federal de Minas Gerais (UFMG)Santa Casa de Belo HorizonteUniversidade Estadual Paulista (Unesp)Rodrigues, Kathryna FontanaPietrani, Nathalia TeixeiraBosco, Adriana AparecidaCampos, Fernanda Magalhães FreireSandrim, Valéria Cristina [UNESP]Gomes, Karina Braga2018-12-11T16:47:13Z2018-12-11T16:47:13Z2017-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article438-446application/pdfhttp://dx.doi.org/10.1590/2359-3997000000254Archives of Endocrinology and Metabolism, v. 61, n. 5, p. 438-446, 2017.2359-42922359-3997http://hdl.handle.net/11449/16970110.1590/2359-3997000000254S2359-399720170005004382-s2.0-85018943849S2359-39972017000500438.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArchives of Endocrinology and Metabolism0,5050,505info:eu-repo/semantics/openAccess2023-12-04T06:18:35Zoai:repositorio.unesp.br:11449/169701Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:30:40.048059Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
IL-6, TNF-α, and IL-10 levels/ polymorphisms and their association with type 2 diabetes mellitus and obesity in Brazilian individuals |
title |
IL-6, TNF-α, and IL-10 levels/ polymorphisms and their association with type 2 diabetes mellitus and obesity in Brazilian individuals |
spellingShingle |
IL-6, TNF-α, and IL-10 levels/ polymorphisms and their association with type 2 diabetes mellitus and obesity in Brazilian individuals Rodrigues, Kathryna Fontana Interleukin-10 Interleukin-6 Polymorphisms Tumor necrosis factor-alpha Type 2 diabetes mellitus |
title_short |
IL-6, TNF-α, and IL-10 levels/ polymorphisms and their association with type 2 diabetes mellitus and obesity in Brazilian individuals |
title_full |
IL-6, TNF-α, and IL-10 levels/ polymorphisms and their association with type 2 diabetes mellitus and obesity in Brazilian individuals |
title_fullStr |
IL-6, TNF-α, and IL-10 levels/ polymorphisms and their association with type 2 diabetes mellitus and obesity in Brazilian individuals |
title_full_unstemmed |
IL-6, TNF-α, and IL-10 levels/ polymorphisms and their association with type 2 diabetes mellitus and obesity in Brazilian individuals |
title_sort |
IL-6, TNF-α, and IL-10 levels/ polymorphisms and their association with type 2 diabetes mellitus and obesity in Brazilian individuals |
author |
Rodrigues, Kathryna Fontana |
author_facet |
Rodrigues, Kathryna Fontana Pietrani, Nathalia Teixeira Bosco, Adriana Aparecida Campos, Fernanda Magalhães Freire Sandrim, Valéria Cristina [UNESP] Gomes, Karina Braga |
author_role |
author |
author2 |
Pietrani, Nathalia Teixeira Bosco, Adriana Aparecida Campos, Fernanda Magalhães Freire Sandrim, Valéria Cristina [UNESP] Gomes, Karina Braga |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de Minas Gerais (UFMG) Santa Casa de Belo Horizonte Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Rodrigues, Kathryna Fontana Pietrani, Nathalia Teixeira Bosco, Adriana Aparecida Campos, Fernanda Magalhães Freire Sandrim, Valéria Cristina [UNESP] Gomes, Karina Braga |
dc.subject.por.fl_str_mv |
Interleukin-10 Interleukin-6 Polymorphisms Tumor necrosis factor-alpha Type 2 diabetes mellitus |
topic |
Interleukin-10 Interleukin-6 Polymorphisms Tumor necrosis factor-alpha Type 2 diabetes mellitus |
description |
Objective: This study aimed to investigate the association of plasma TNF-α, IL-6, and lL-10 levels and cytokine gene polymorphisms [TNF-α (-308 G→A), IL-6 (-174 C→G) and IL-10 (-1082 A→G, -819 T→C and -592 A→C)] in type 2 diabetes mellitus (T2DM) and obese patients. Subjects and methods: One hundred and two T2DM patients and 62 controls were included in this study. Cytokine plasma levels were measured by the Cytometric Bead Array method. Genotyping was carried out by the polymerase chain reaction. Results: IL-6 levels were significantly different between T2DM patients and controls. Interestingly, IL-6 levels were higher in T2DM patients with BMI > 30 kg/m2 compared with other patients and obese controls. The genotype and allele frequencies were similar between patients and controls. In the T2DM group, the SNP IL-10 -819 T/C showed a difference between the cytokine level and genotypes: IL-10 level in the TT genotype was significantly higher when compared to CC genotype. Conclusions: These results suggest an association between IL-6 levels and obesity, and IL-10 levels and the SNP -819 T/C in T2DM. Knowledge of these variants in T2DM might contribute to a better understanding of the role of inflammation in the etiology and progression of this disease. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-10-01 2018-12-11T16:47:13Z 2018-12-11T16:47:13Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/2359-3997000000254 Archives of Endocrinology and Metabolism, v. 61, n. 5, p. 438-446, 2017. 2359-4292 2359-3997 http://hdl.handle.net/11449/169701 10.1590/2359-3997000000254 S2359-39972017000500438 2-s2.0-85018943849 S2359-39972017000500438.pdf |
url |
http://dx.doi.org/10.1590/2359-3997000000254 http://hdl.handle.net/11449/169701 |
identifier_str_mv |
Archives of Endocrinology and Metabolism, v. 61, n. 5, p. 438-446, 2017. 2359-4292 2359-3997 10.1590/2359-3997000000254 S2359-39972017000500438 2-s2.0-85018943849 S2359-39972017000500438.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Archives of Endocrinology and Metabolism 0,505 0,505 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
438-446 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129079447126016 |