IL-6, TNF-α, and IL-10 levels/ polymorphisms and their association with type 2 diabetes mellitus and obesity in Brazilian individuals

Detalhes bibliográficos
Autor(a) principal: Rodrigues, Kathryna Fontana
Data de Publicação: 2017
Outros Autores: Pietrani, Nathalia Teixeira, Bosco, Adriana Aparecida, Campos, Fernanda Magalhães Freire, Sandrim, Valéria Cristina [UNESP], Gomes, Karina Braga
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/2359-3997000000254
http://hdl.handle.net/11449/169701
Resumo: Objective: This study aimed to investigate the association of plasma TNF-α, IL-6, and lL-10 levels and cytokine gene polymorphisms [TNF-α (-308 G→A), IL-6 (-174 C→G) and IL-10 (-1082 A→G, -819 T→C and -592 A→C)] in type 2 diabetes mellitus (T2DM) and obese patients. Subjects and methods: One hundred and two T2DM patients and 62 controls were included in this study. Cytokine plasma levels were measured by the Cytometric Bead Array method. Genotyping was carried out by the polymerase chain reaction. Results: IL-6 levels were significantly different between T2DM patients and controls. Interestingly, IL-6 levels were higher in T2DM patients with BMI > 30 kg/m2 compared with other patients and obese controls. The genotype and allele frequencies were similar between patients and controls. In the T2DM group, the SNP IL-10 -819 T/C showed a difference between the cytokine level and genotypes: IL-10 level in the TT genotype was significantly higher when compared to CC genotype. Conclusions: These results suggest an association between IL-6 levels and obesity, and IL-10 levels and the SNP -819 T/C in T2DM. Knowledge of these variants in T2DM might contribute to a better understanding of the role of inflammation in the etiology and progression of this disease.
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spelling IL-6, TNF-α, and IL-10 levels/ polymorphisms and their association with type 2 diabetes mellitus and obesity in Brazilian individualsInterleukin-10Interleukin-6PolymorphismsTumor necrosis factor-alphaType 2 diabetes mellitusObjective: This study aimed to investigate the association of plasma TNF-α, IL-6, and lL-10 levels and cytokine gene polymorphisms [TNF-α (-308 G→A), IL-6 (-174 C→G) and IL-10 (-1082 A→G, -819 T→C and -592 A→C)] in type 2 diabetes mellitus (T2DM) and obese patients. Subjects and methods: One hundred and two T2DM patients and 62 controls were included in this study. Cytokine plasma levels were measured by the Cytometric Bead Array method. Genotyping was carried out by the polymerase chain reaction. Results: IL-6 levels were significantly different between T2DM patients and controls. Interestingly, IL-6 levels were higher in T2DM patients with BMI > 30 kg/m2 compared with other patients and obese controls. The genotype and allele frequencies were similar between patients and controls. In the T2DM group, the SNP IL-10 -819 T/C showed a difference between the cytokine level and genotypes: IL-10 level in the TT genotype was significantly higher when compared to CC genotype. Conclusions: These results suggest an association between IL-6 levels and obesity, and IL-10 levels and the SNP -819 T/C in T2DM. Knowledge of these variants in T2DM might contribute to a better understanding of the role of inflammation in the etiology and progression of this disease.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)Universidade Federal de Minas GeraisInstituto de Ciências Biológicas Universidade Federal de Minas Gerais (UFMG)Instituto de Ensino e Pesquisa Santa Casa de Belo HorizonteFaculdade de Farmácia UFMGInstituto de Biociências Universidade Estadual Paulista Júlio de Mesquita Filho (Unesp)Instituto de Biociências Universidade Estadual Paulista Júlio de Mesquita Filho (Unesp)Universidade Federal de Minas Gerais (UFMG)Santa Casa de Belo HorizonteUniversidade Estadual Paulista (Unesp)Rodrigues, Kathryna FontanaPietrani, Nathalia TeixeiraBosco, Adriana AparecidaCampos, Fernanda Magalhães FreireSandrim, Valéria Cristina [UNESP]Gomes, Karina Braga2018-12-11T16:47:13Z2018-12-11T16:47:13Z2017-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article438-446application/pdfhttp://dx.doi.org/10.1590/2359-3997000000254Archives of Endocrinology and Metabolism, v. 61, n. 5, p. 438-446, 2017.2359-42922359-3997http://hdl.handle.net/11449/16970110.1590/2359-3997000000254S2359-399720170005004382-s2.0-85018943849S2359-39972017000500438.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArchives of Endocrinology and Metabolism0,5050,505info:eu-repo/semantics/openAccess2023-12-04T06:18:35Zoai:repositorio.unesp.br:11449/169701Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:30:40.048059Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv IL-6, TNF-α, and IL-10 levels/ polymorphisms and their association with type 2 diabetes mellitus and obesity in Brazilian individuals
title IL-6, TNF-α, and IL-10 levels/ polymorphisms and their association with type 2 diabetes mellitus and obesity in Brazilian individuals
spellingShingle IL-6, TNF-α, and IL-10 levels/ polymorphisms and their association with type 2 diabetes mellitus and obesity in Brazilian individuals
Rodrigues, Kathryna Fontana
Interleukin-10
Interleukin-6
Polymorphisms
Tumor necrosis factor-alpha
Type 2 diabetes mellitus
title_short IL-6, TNF-α, and IL-10 levels/ polymorphisms and their association with type 2 diabetes mellitus and obesity in Brazilian individuals
title_full IL-6, TNF-α, and IL-10 levels/ polymorphisms and their association with type 2 diabetes mellitus and obesity in Brazilian individuals
title_fullStr IL-6, TNF-α, and IL-10 levels/ polymorphisms and their association with type 2 diabetes mellitus and obesity in Brazilian individuals
title_full_unstemmed IL-6, TNF-α, and IL-10 levels/ polymorphisms and their association with type 2 diabetes mellitus and obesity in Brazilian individuals
title_sort IL-6, TNF-α, and IL-10 levels/ polymorphisms and their association with type 2 diabetes mellitus and obesity in Brazilian individuals
author Rodrigues, Kathryna Fontana
author_facet Rodrigues, Kathryna Fontana
Pietrani, Nathalia Teixeira
Bosco, Adriana Aparecida
Campos, Fernanda Magalhães Freire
Sandrim, Valéria Cristina [UNESP]
Gomes, Karina Braga
author_role author
author2 Pietrani, Nathalia Teixeira
Bosco, Adriana Aparecida
Campos, Fernanda Magalhães Freire
Sandrim, Valéria Cristina [UNESP]
Gomes, Karina Braga
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de Minas Gerais (UFMG)
Santa Casa de Belo Horizonte
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Rodrigues, Kathryna Fontana
Pietrani, Nathalia Teixeira
Bosco, Adriana Aparecida
Campos, Fernanda Magalhães Freire
Sandrim, Valéria Cristina [UNESP]
Gomes, Karina Braga
dc.subject.por.fl_str_mv Interleukin-10
Interleukin-6
Polymorphisms
Tumor necrosis factor-alpha
Type 2 diabetes mellitus
topic Interleukin-10
Interleukin-6
Polymorphisms
Tumor necrosis factor-alpha
Type 2 diabetes mellitus
description Objective: This study aimed to investigate the association of plasma TNF-α, IL-6, and lL-10 levels and cytokine gene polymorphisms [TNF-α (-308 G→A), IL-6 (-174 C→G) and IL-10 (-1082 A→G, -819 T→C and -592 A→C)] in type 2 diabetes mellitus (T2DM) and obese patients. Subjects and methods: One hundred and two T2DM patients and 62 controls were included in this study. Cytokine plasma levels were measured by the Cytometric Bead Array method. Genotyping was carried out by the polymerase chain reaction. Results: IL-6 levels were significantly different between T2DM patients and controls. Interestingly, IL-6 levels were higher in T2DM patients with BMI > 30 kg/m2 compared with other patients and obese controls. The genotype and allele frequencies were similar between patients and controls. In the T2DM group, the SNP IL-10 -819 T/C showed a difference between the cytokine level and genotypes: IL-10 level in the TT genotype was significantly higher when compared to CC genotype. Conclusions: These results suggest an association between IL-6 levels and obesity, and IL-10 levels and the SNP -819 T/C in T2DM. Knowledge of these variants in T2DM might contribute to a better understanding of the role of inflammation in the etiology and progression of this disease.
publishDate 2017
dc.date.none.fl_str_mv 2017-10-01
2018-12-11T16:47:13Z
2018-12-11T16:47:13Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/2359-3997000000254
Archives of Endocrinology and Metabolism, v. 61, n. 5, p. 438-446, 2017.
2359-4292
2359-3997
http://hdl.handle.net/11449/169701
10.1590/2359-3997000000254
S2359-39972017000500438
2-s2.0-85018943849
S2359-39972017000500438.pdf
url http://dx.doi.org/10.1590/2359-3997000000254
http://hdl.handle.net/11449/169701
identifier_str_mv Archives of Endocrinology and Metabolism, v. 61, n. 5, p. 438-446, 2017.
2359-4292
2359-3997
10.1590/2359-3997000000254
S2359-39972017000500438
2-s2.0-85018943849
S2359-39972017000500438.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Archives of Endocrinology and Metabolism
0,505
0,505
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 438-446
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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