A trans10-18:1 enriched fraction from beef fed a barley grain-based diet induces lipogenic gene expression and reduces viability of HepG2 cells

Detalhes bibliográficos
Autor(a) principal: Vahmani, Payam
Data de Publicação: 2016
Outros Autores: Meadus, William J., da Silva, Maria L.P. [UNESP], Mitchell, Alec D., Mapiye, Cletos, Duff, Pascale, Rolland, David C., Dugan, Michael E.R.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.bbrep.2016.05.018
http://hdl.handle.net/11449/220632
Resumo: Beef fat is a natural source of trans (t) fatty acids, and is typically enriched with either t10-18:1 or t11-18:1. Little is known about the bioactivity of individual t-18:1 isomers, and the present study compared the effects of t9-18:1, cis (c)9-18:1 and trans (t)-18:1 fractions isolated from beef fat enriched with either t10-18:1 (HT10) or t11-18:1 (HT11). All 18:1 isomers resulted in reduced human liver (HepG2) cell viability relative to control. Both c9-18:1 and HT11were the least toxic, t9-18:1had dose response increased toxicity, and HT10 had the greatest toxicity (P<0.05). Incorporation of t18:1 isomers was 1.8-2.5 fold greater in triacylglycerol (TG) than phospholipids (PL), whereas δ9 desaturation products were selectively incorporated into PL. Culturing HepG2 cells with t9-18:1 and HT10 increased (P<0.05) the δ9 desaturation index (c9-16:1/16:0) compared to other fatty acid treatments. HT10 and t9-18:1 also increased expression of lipogenic genes (FAS, SCD1, HMGCR and SREBP2) compared to control (P<0.05), whereas c9-18:1 and HT11 did not affect the expression of these genes. Our results suggest effects of HT11 and c9-18:1 were similar to BSA control, whereas HT10 and t-9 18:1 (i.e. the predominant trans fatty acid isomer found in partially hydrogenated vegetable oils) were more cytotoxic and led to greater expression of lipogenic genes.
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spelling A trans10-18:1 enriched fraction from beef fed a barley grain-based diet induces lipogenic gene expression and reduces viability of HepG2 cellsBeefCell cultureCytotoxicityFatty acid metabolismLiverTrans fatty acidsBeef fat is a natural source of trans (t) fatty acids, and is typically enriched with either t10-18:1 or t11-18:1. Little is known about the bioactivity of individual t-18:1 isomers, and the present study compared the effects of t9-18:1, cis (c)9-18:1 and trans (t)-18:1 fractions isolated from beef fat enriched with either t10-18:1 (HT10) or t11-18:1 (HT11). All 18:1 isomers resulted in reduced human liver (HepG2) cell viability relative to control. Both c9-18:1 and HT11were the least toxic, t9-18:1had dose response increased toxicity, and HT10 had the greatest toxicity (P<0.05). Incorporation of t18:1 isomers was 1.8-2.5 fold greater in triacylglycerol (TG) than phospholipids (PL), whereas δ9 desaturation products were selectively incorporated into PL. Culturing HepG2 cells with t9-18:1 and HT10 increased (P<0.05) the δ9 desaturation index (c9-16:1/16:0) compared to other fatty acid treatments. HT10 and t9-18:1 also increased expression of lipogenic genes (FAS, SCD1, HMGCR and SREBP2) compared to control (P<0.05), whereas c9-18:1 and HT11 did not affect the expression of these genes. Our results suggest effects of HT11 and c9-18:1 were similar to BSA control, whereas HT10 and t-9 18:1 (i.e. the predominant trans fatty acid isomer found in partially hydrogenated vegetable oils) were more cytotoxic and led to greater expression of lipogenic genes.Lacombe Research and Development Centre Agriculture and Agri-Food CanadaFaculty of Veterinary and Agricultural Sciences São Paulo State UniversityAculty of Science University of AlbertaDepartment of Animal Sciences Faculty of AgriSciences Stellenbosch University, P. Bag X1Faculty of Veterinary and Agricultural Sciences São Paulo State UniversityAgriculture and Agri-Food CanadaUniversidade Estadual Paulista (UNESP)University of AlbertaStellenbosch UniversityVahmani, PayamMeadus, William J.da Silva, Maria L.P. [UNESP]Mitchell, Alec D.Mapiye, CletosDuff, PascaleRolland, David C.Dugan, Michael E.R.2022-04-28T19:03:38Z2022-04-28T19:03:38Z2016-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article84-90http://dx.doi.org/10.1016/j.bbrep.2016.05.018Biochemistry and Biophysics Reports, v. 7, p. 84-90.2405-5808http://hdl.handle.net/11449/22063210.1016/j.bbrep.2016.05.0182-s2.0-84973154893Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiochemistry and Biophysics Reportsinfo:eu-repo/semantics/openAccess2022-04-28T19:03:38Zoai:repositorio.unesp.br:11449/220632Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:39:01.175380Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv A trans10-18:1 enriched fraction from beef fed a barley grain-based diet induces lipogenic gene expression and reduces viability of HepG2 cells
title A trans10-18:1 enriched fraction from beef fed a barley grain-based diet induces lipogenic gene expression and reduces viability of HepG2 cells
spellingShingle A trans10-18:1 enriched fraction from beef fed a barley grain-based diet induces lipogenic gene expression and reduces viability of HepG2 cells
Vahmani, Payam
Beef
Cell culture
Cytotoxicity
Fatty acid metabolism
Liver
Trans fatty acids
title_short A trans10-18:1 enriched fraction from beef fed a barley grain-based diet induces lipogenic gene expression and reduces viability of HepG2 cells
title_full A trans10-18:1 enriched fraction from beef fed a barley grain-based diet induces lipogenic gene expression and reduces viability of HepG2 cells
title_fullStr A trans10-18:1 enriched fraction from beef fed a barley grain-based diet induces lipogenic gene expression and reduces viability of HepG2 cells
title_full_unstemmed A trans10-18:1 enriched fraction from beef fed a barley grain-based diet induces lipogenic gene expression and reduces viability of HepG2 cells
title_sort A trans10-18:1 enriched fraction from beef fed a barley grain-based diet induces lipogenic gene expression and reduces viability of HepG2 cells
author Vahmani, Payam
author_facet Vahmani, Payam
Meadus, William J.
da Silva, Maria L.P. [UNESP]
Mitchell, Alec D.
Mapiye, Cletos
Duff, Pascale
Rolland, David C.
Dugan, Michael E.R.
author_role author
author2 Meadus, William J.
da Silva, Maria L.P. [UNESP]
Mitchell, Alec D.
Mapiye, Cletos
Duff, Pascale
Rolland, David C.
Dugan, Michael E.R.
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Agriculture and Agri-Food Canada
Universidade Estadual Paulista (UNESP)
University of Alberta
Stellenbosch University
dc.contributor.author.fl_str_mv Vahmani, Payam
Meadus, William J.
da Silva, Maria L.P. [UNESP]
Mitchell, Alec D.
Mapiye, Cletos
Duff, Pascale
Rolland, David C.
Dugan, Michael E.R.
dc.subject.por.fl_str_mv Beef
Cell culture
Cytotoxicity
Fatty acid metabolism
Liver
Trans fatty acids
topic Beef
Cell culture
Cytotoxicity
Fatty acid metabolism
Liver
Trans fatty acids
description Beef fat is a natural source of trans (t) fatty acids, and is typically enriched with either t10-18:1 or t11-18:1. Little is known about the bioactivity of individual t-18:1 isomers, and the present study compared the effects of t9-18:1, cis (c)9-18:1 and trans (t)-18:1 fractions isolated from beef fat enriched with either t10-18:1 (HT10) or t11-18:1 (HT11). All 18:1 isomers resulted in reduced human liver (HepG2) cell viability relative to control. Both c9-18:1 and HT11were the least toxic, t9-18:1had dose response increased toxicity, and HT10 had the greatest toxicity (P<0.05). Incorporation of t18:1 isomers was 1.8-2.5 fold greater in triacylglycerol (TG) than phospholipids (PL), whereas δ9 desaturation products were selectively incorporated into PL. Culturing HepG2 cells with t9-18:1 and HT10 increased (P<0.05) the δ9 desaturation index (c9-16:1/16:0) compared to other fatty acid treatments. HT10 and t9-18:1 also increased expression of lipogenic genes (FAS, SCD1, HMGCR and SREBP2) compared to control (P<0.05), whereas c9-18:1 and HT11 did not affect the expression of these genes. Our results suggest effects of HT11 and c9-18:1 were similar to BSA control, whereas HT10 and t-9 18:1 (i.e. the predominant trans fatty acid isomer found in partially hydrogenated vegetable oils) were more cytotoxic and led to greater expression of lipogenic genes.
publishDate 2016
dc.date.none.fl_str_mv 2016-09-01
2022-04-28T19:03:38Z
2022-04-28T19:03:38Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.bbrep.2016.05.018
Biochemistry and Biophysics Reports, v. 7, p. 84-90.
2405-5808
http://hdl.handle.net/11449/220632
10.1016/j.bbrep.2016.05.018
2-s2.0-84973154893
url http://dx.doi.org/10.1016/j.bbrep.2016.05.018
http://hdl.handle.net/11449/220632
identifier_str_mv Biochemistry and Biophysics Reports, v. 7, p. 84-90.
2405-5808
10.1016/j.bbrep.2016.05.018
2-s2.0-84973154893
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Biochemistry and Biophysics Reports
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 84-90
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129231898542080

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