Anatomical organization of urocortin 3-Synthesizing neurons and immunoreactive terminals in the central nervous system of non-human primates [Sapajus spp.]
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3389/fnana.2017.00057 http://hdl.handle.net/11449/170113 |
Resumo: | Urocortin 3 (UCN3) is a neuropeptide member of the corticotropin-releasing factor (CRF) peptide family that acts as a selective endogenous ligand for the CRF, subtype 2 (CRF2) receptor. Immunohistochemistry and in situ hybridization data from rodents revealed UCN3-containing neurons in discrete regions of the central nervous system (CNS), such as the medial preoptic nucleus, the rostral perifornical area (PFA), the medial nucleus of the amygdala and the superior paraolivary nucleus. UCN3-immunoreactive (UCN3-ir) terminals are distributed throughout regions that mostly overlap with regions of CRF2 messenger RNA (mRNA) expression. Currently, no similar mapping exists for non-human primates. To better understand the role of this neuropeptide, we aimed to study the UCN3 distribution in the brains of New World monkeys of the Sapajus genus. To this end, we analyzed the gene and peptide sequences in these animals and performed immunohistochemistry and in situ hybridization to identify UCN3 synthesis sites and to determine the distribution of UCN3-ir terminals. The sequencing of the Sapajus spp. UCN3-coding gene revealed 88% and 65% identity to the human and rat counterparts, respectively. Additionally, using a probe generated from monkey cDNA and an antiserum raised against human UCN3, we found that labeled cells are mainly located in the hypothalamic and limbic regions. UCN3-ir axons and terminals are primarily distributed in the ventromedial hypothalamic nucleus (VMH) and the lateral septal nucleus (LS). Our results demonstrate that UCN3-producing neurons in the CNS of monkeys are phylogenetically conserved compared to those of the rodent brain, that the distribution of fibers agrees with the distribution of CRF2 in other primates and that there is anatomical evidence for the participation of UCN3 in neuroendocrine control in primates. |
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Anatomical organization of urocortin 3-Synthesizing neurons and immunoreactive terminals in the central nervous system of non-human primates [Sapajus spp.]Autonomic nervous systemCorticotropin-releasing factorCorticotropin-releasing factor receptorsMonkey brainParaventricular nucleus of the hypothalamusStress responseUrocortin 3 (UCN3) is a neuropeptide member of the corticotropin-releasing factor (CRF) peptide family that acts as a selective endogenous ligand for the CRF, subtype 2 (CRF2) receptor. Immunohistochemistry and in situ hybridization data from rodents revealed UCN3-containing neurons in discrete regions of the central nervous system (CNS), such as the medial preoptic nucleus, the rostral perifornical area (PFA), the medial nucleus of the amygdala and the superior paraolivary nucleus. UCN3-immunoreactive (UCN3-ir) terminals are distributed throughout regions that mostly overlap with regions of CRF2 messenger RNA (mRNA) expression. Currently, no similar mapping exists for non-human primates. To better understand the role of this neuropeptide, we aimed to study the UCN3 distribution in the brains of New World monkeys of the Sapajus genus. To this end, we analyzed the gene and peptide sequences in these animals and performed immunohistochemistry and in situ hybridization to identify UCN3 synthesis sites and to determine the distribution of UCN3-ir terminals. The sequencing of the Sapajus spp. UCN3-coding gene revealed 88% and 65% identity to the human and rat counterparts, respectively. Additionally, using a probe generated from monkey cDNA and an antiserum raised against human UCN3, we found that labeled cells are mainly located in the hypothalamic and limbic regions. UCN3-ir axons and terminals are primarily distributed in the ventromedial hypothalamic nucleus (VMH) and the lateral septal nucleus (LS). Our results demonstrate that UCN3-producing neurons in the CNS of monkeys are phylogenetically conserved compared to those of the rodent brain, that the distribution of fibers agrees with the distribution of CRF2 in other primates and that there is anatomical evidence for the participation of UCN3 in neuroendocrine control in primates.Cement Association of CanadaConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Department of Anatomy Institute of Biomedical Sciences University of São PauloCenter for Neuroscience and Behaviour Institute of Psychology University of São PauloDepartment of Anatomy Santa Marcelina Medical SchoolDepartment of Basic Sciences São Paulo State University UNESPLaboratory of Cellular Structure and Function Department of Anatomy Institute of Biomedical Sciences University of São PauloInstitute of Biosciences UNESP-São Paulo State UniversityLaboratory of Neuroendocrinology Department of Cell and Systems Biology University of TorontoDepartment of Basic Sciences São Paulo State University UNESPInstitute of Biosciences UNESP-São Paulo State UniversityCement Association of Canada: 2012/03067-6Universidade de São Paulo (USP)Santa Marcelina Medical SchoolUniversidade Estadual Paulista (Unesp)University of TorontoBattagello, Daniella S.Diniz, Giovanne B.Candido, Paulo L.da Silva, Joelcimar M.de Oliveira, Amanda R.Torres da Silva, Kelly R. [UNESP]Lotfi, Claudimara F. P.de Oliveira, José A. [UNESP]Sita, Luciane V.Casatti, Cláudio A. [UNESP]Lovejoy, David A.Bittencourt, Jackson C.2018-12-11T16:49:20Z2018-12-11T16:49:20Z2017-07-24info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.3389/fnana.2017.00057Frontiers in Neuroanatomy, v. 11.1662-5129http://hdl.handle.net/11449/17011310.3389/fnana.2017.000572-s2.0-850291440072-s2.0-85029144007.pdf84874626269318770000-0001-5650-7343Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFrontiers in Neuroanatomy1,999info:eu-repo/semantics/openAccess2023-12-10T06:21:57Zoai:repositorio.unesp.br:11449/170113Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:58:08.115255Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Anatomical organization of urocortin 3-Synthesizing neurons and immunoreactive terminals in the central nervous system of non-human primates [Sapajus spp.] |
title |
Anatomical organization of urocortin 3-Synthesizing neurons and immunoreactive terminals in the central nervous system of non-human primates [Sapajus spp.] |
spellingShingle |
Anatomical organization of urocortin 3-Synthesizing neurons and immunoreactive terminals in the central nervous system of non-human primates [Sapajus spp.] Battagello, Daniella S. Autonomic nervous system Corticotropin-releasing factor Corticotropin-releasing factor receptors Monkey brain Paraventricular nucleus of the hypothalamus Stress response |
title_short |
Anatomical organization of urocortin 3-Synthesizing neurons and immunoreactive terminals in the central nervous system of non-human primates [Sapajus spp.] |
title_full |
Anatomical organization of urocortin 3-Synthesizing neurons and immunoreactive terminals in the central nervous system of non-human primates [Sapajus spp.] |
title_fullStr |
Anatomical organization of urocortin 3-Synthesizing neurons and immunoreactive terminals in the central nervous system of non-human primates [Sapajus spp.] |
title_full_unstemmed |
Anatomical organization of urocortin 3-Synthesizing neurons and immunoreactive terminals in the central nervous system of non-human primates [Sapajus spp.] |
title_sort |
Anatomical organization of urocortin 3-Synthesizing neurons and immunoreactive terminals in the central nervous system of non-human primates [Sapajus spp.] |
author |
Battagello, Daniella S. |
author_facet |
Battagello, Daniella S. Diniz, Giovanne B. Candido, Paulo L. da Silva, Joelcimar M. de Oliveira, Amanda R. Torres da Silva, Kelly R. [UNESP] Lotfi, Claudimara F. P. de Oliveira, José A. [UNESP] Sita, Luciane V. Casatti, Cláudio A. [UNESP] Lovejoy, David A. Bittencourt, Jackson C. |
author_role |
author |
author2 |
Diniz, Giovanne B. Candido, Paulo L. da Silva, Joelcimar M. de Oliveira, Amanda R. Torres da Silva, Kelly R. [UNESP] Lotfi, Claudimara F. P. de Oliveira, José A. [UNESP] Sita, Luciane V. Casatti, Cláudio A. [UNESP] Lovejoy, David A. Bittencourt, Jackson C. |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Santa Marcelina Medical School Universidade Estadual Paulista (Unesp) University of Toronto |
dc.contributor.author.fl_str_mv |
Battagello, Daniella S. Diniz, Giovanne B. Candido, Paulo L. da Silva, Joelcimar M. de Oliveira, Amanda R. Torres da Silva, Kelly R. [UNESP] Lotfi, Claudimara F. P. de Oliveira, José A. [UNESP] Sita, Luciane V. Casatti, Cláudio A. [UNESP] Lovejoy, David A. Bittencourt, Jackson C. |
dc.subject.por.fl_str_mv |
Autonomic nervous system Corticotropin-releasing factor Corticotropin-releasing factor receptors Monkey brain Paraventricular nucleus of the hypothalamus Stress response |
topic |
Autonomic nervous system Corticotropin-releasing factor Corticotropin-releasing factor receptors Monkey brain Paraventricular nucleus of the hypothalamus Stress response |
description |
Urocortin 3 (UCN3) is a neuropeptide member of the corticotropin-releasing factor (CRF) peptide family that acts as a selective endogenous ligand for the CRF, subtype 2 (CRF2) receptor. Immunohistochemistry and in situ hybridization data from rodents revealed UCN3-containing neurons in discrete regions of the central nervous system (CNS), such as the medial preoptic nucleus, the rostral perifornical area (PFA), the medial nucleus of the amygdala and the superior paraolivary nucleus. UCN3-immunoreactive (UCN3-ir) terminals are distributed throughout regions that mostly overlap with regions of CRF2 messenger RNA (mRNA) expression. Currently, no similar mapping exists for non-human primates. To better understand the role of this neuropeptide, we aimed to study the UCN3 distribution in the brains of New World monkeys of the Sapajus genus. To this end, we analyzed the gene and peptide sequences in these animals and performed immunohistochemistry and in situ hybridization to identify UCN3 synthesis sites and to determine the distribution of UCN3-ir terminals. The sequencing of the Sapajus spp. UCN3-coding gene revealed 88% and 65% identity to the human and rat counterparts, respectively. Additionally, using a probe generated from monkey cDNA and an antiserum raised against human UCN3, we found that labeled cells are mainly located in the hypothalamic and limbic regions. UCN3-ir axons and terminals are primarily distributed in the ventromedial hypothalamic nucleus (VMH) and the lateral septal nucleus (LS). Our results demonstrate that UCN3-producing neurons in the CNS of monkeys are phylogenetically conserved compared to those of the rodent brain, that the distribution of fibers agrees with the distribution of CRF2 in other primates and that there is anatomical evidence for the participation of UCN3 in neuroendocrine control in primates. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-07-24 2018-12-11T16:49:20Z 2018-12-11T16:49:20Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3389/fnana.2017.00057 Frontiers in Neuroanatomy, v. 11. 1662-5129 http://hdl.handle.net/11449/170113 10.3389/fnana.2017.00057 2-s2.0-85029144007 2-s2.0-85029144007.pdf 8487462626931877 0000-0001-5650-7343 |
url |
http://dx.doi.org/10.3389/fnana.2017.00057 http://hdl.handle.net/11449/170113 |
identifier_str_mv |
Frontiers in Neuroanatomy, v. 11. 1662-5129 10.3389/fnana.2017.00057 2-s2.0-85029144007 2-s2.0-85029144007.pdf 8487462626931877 0000-0001-5650-7343 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Frontiers in Neuroanatomy 1,999 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129144657018880 |